Restriction map for
pFN21ASDA1036
(Circular) (Six-base) MAPSORT of: pFN21ASDA1036.seq Check: 1698 from: 1 to: 5824
pFN21ASDA1036 5824 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2366 3117 3167 1937
Size: 429 751 50 4594
Fragments arranged by size:
4594 751 429 50
Acc65I G'GTAC_C
Cuts at: 3102 3145 3102
Size: 43 5781
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3887 5271 5539 1632
Size: 2255 1384 268 1917
Fragments arranged by size:
2255 1917 1384 268
AfeI AGC'GCT
Cuts at: 2402 2585 2402
Size: 183 5641
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 2260 4723 5700 1203
Size: 1057 2463 977 1327
Fragments arranged by size:
2463 1327 1057 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2954 3007 2954
Size: 53 5771
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 2550 3133 3359 3511 5620 1493
Size: 1057 583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1057 583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 3052 3149 1870
Size: 73 1109 97 4545
Fragments arranged by size:
4545 1109 97 73
BamHI G'GATC_C
Cuts at: 2304 3154 3521 2304
Size: 850 367 4607
Fragments arranged by size:
4607 850 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 3102 3145 4600 618
Size: 325 217 627 276 1039 43 1455 1842
Fragments arranged by size:
1842 1455 1039 627 325 276 217 43
BanII G_rGCy'C
Cuts at: 1391 2739 2954 3007 3078 3143 1391
Size: 1348 215 53 71 65 4072
Fragments arranged by size:
4072 1348 215 71 65 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3071 928
Size: 971 1172 3681
Fragments arranged by size:
3681 1172 971
BbvCI CC'TCA_GC
Cuts at: 2134 2134
Size: 5824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4015 4049 1903
Size: 34 2078 34 3678
Fragments arranged by size:
3678 2078 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 2729 3241 2729
Size: 512 5312
Bme1580I G_kGCm'C
Cuts at: 1790 2954 3007 3885 5041 1790
Size: 1164 53 878 1156 2573
Fragments arranged by size:
2573 1164 1156 878 53
BmgBI CAC'GTC
Cuts at: 1318 2866 1318
Size: 1548 4276
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3001 3555 4513
Size: 416 53 83 399 24 1584 554 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1584 958 554 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2484 2484
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 2924 4484 1239
Size: 1361 324 1560 2579
Fragments arranged by size:
2579 1560 1361 324
Bpu10I CC'TnA_GC
Cuts at: 2134 2379 2134
Size: 245 5579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2768 2915 3949 4814 5112 2768
Size: 147 1034 865 298 3480
Fragments arranged by size:
3480 1034 865 298 147
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 4487 882
Size: 617 612 2376 2219
Fragments arranged by size:
2376 2219 617 612
BsaAI yAC'GTr
Cuts at: 493 1204 2263 2779 3121 3615 493
Size: 711 1059 516 342 494 2702
Fragments arranged by size:
2702 1059 711 516 494 342
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 3631 4013 4648
Size: 53 83 186 936 660 1438 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1451 1438 936 660 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2973 4257 4655 4929 5076 5487 2973
Size: 1284 398 274 147 411 3310
Fragments arranged by size:
3310 1284 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 2938 2968 1178
Size: 30 165 30 874 30 631 30 4034
Fragments arranged by size:
4034 874 631 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 1186
Size: 979 4845
BseYI C'CCAG_C
Cuts at: 2045 2641 2674 2797 5027 5523 2045
Size: 596 33 123 2230 496 2346
Fragments arranged by size:
2346 2230 596 496 123 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2417 1163
Size: 573 681 4570
Fragments arranged by size:
4570 681 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2767 3274 4035 5063 1540
Size: 275 186 766 507 761 1028 2301
Fragments arranged by size:
2301 1028 766 761 507 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 2784 3885 3970 5041 2739
Size: 45 1101 85 1071 3522
Fragments arranged by size:
3522 1101 1071 85 45
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
BsmBI CGTCTCn'nnnn_
Cuts at: 2178 2178
Size: 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2739 2784 2954 3007 3078 3143 3885
Size: 399 949 45 170 53 71 65 742
Cuts at: 3885 3970 5041 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 949 742 399 170 85 71
65 53 45
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 2331 3179 844
Size: 1343 144 848 3489
Fragments arranged by size:
3489 1343 848 144
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 2703 96
Size: 2607 3217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 2738 3884 4896 2738
Size: 1146 1012 3666
Fragments arranged by size:
3666 1146 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2857 794
Size: 2063 3761
BstBI TT'CG_AA
Cuts at: 2007 4628 2007
Size: 2621 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2282 2282
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 2271 2304 2331 3108 3154 3521 3906 3923
Size: 299 33 27 777 46 367 385 17
Cuts at: 3923 5375 5461 5473 5819 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 777 385 367 346 299 86
46 33 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2308 513
Size: 552 1243 4029
Fragments arranged by size:
4029 1243 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2898 487
Size: 683 56 1672 3413
Fragments arranged by size:
3413 1672 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3340 4134 4154 3043
Size: 297 794 20 4713
Fragments arranged by size:
4713 794 297 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2197 2232 529
Size: 35 1633 35 4121
Fragments arranged by size:
4121 1633 35 35
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DraIII CAC_nnn'GTG
Cuts at: 2638 2830 2857 2638
Size: 192 27 5605
Fragments arranged by size:
5605 192 27
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2192 4831 809
Size: 507 876 2639 1802
Fragments arranged by size:
2639 1802 876 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2085 2265 2490
Size: 54 1425 50 275 18 255 180 225
Cuts at: 2490 2764 3271 4160 5789 8
Size: 274 507 889 1629 43
Fragments arranged by size:
1629 1425 889 507 275 274 255 225
180 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2764 3271 1537
Size: 275 952 507 4090
Fragments arranged by size:
4090 952 507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2166 2453 3754 1353
Size: 801 12 287 1301 3423
Fragments arranged by size:
3423 1301 801 287 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2618 4411 4785 4931 387
Size: 1064 138 1029 1793 374 146 1280
Fragments arranged by size:
1793 1280 1064 1029 374 146 138
EcoICRI GAG'CTC
Cuts at: 2737 2737
Size: 5824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 2924 3887 4484 5271 5539 1239
Size: 393 968 324 963 597 787 268 1524
Fragments arranged by size:
1524 968 963 787 597 393 324 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2245 2472 2492 2245
Size: 227 20 5577
Fragments arranged by size:
5577 227 20
EcoO109I rG'GnC_Cy
Cuts at: 2176 2512 2695 2951 2176
Size: 336 183 256 5049
Fragments arranged by size:
5049 336 256 183
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 5824
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2386 2404 2587 2875 2911 4971 1678
Size: 708 18 183 288 36 2060 2531
Fragments arranged by size:
2531 2060 708 288 183 36 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2938 2970 4465 4497 4539
Size: 32 135 32 1366 32 1495 32 42
Cuts at: 4539 4571 1373
Size: 32 2626
Fragments arranged by size:
2626 1495 1366 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 3168 3414 669
Size: 988 281 271 959 246 3079
Fragments arranged by size:
3079 988 959 281 271 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3106 3149 3106
Size: 43 5781
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2096 4938 5122 1305
Size: 791 2842 184 2007
Fragments arranged by size:
2842 2007 791 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2267 2492 10
Size: 54 1425 343 435 225 3342
Fragments arranged by size:
3342 1425 435 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2280 3782 4141 4300 518
Size: 648 1114 1502 359 159 2042
Fragments arranged by size:
2042 1502 1114 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2081 2769 3001 3240 3917 5065 5310
Size: 455 20 688 232 239 677 1148 245
Cuts at: 5310 1606
Size: 2120
Fragments arranged by size:
2120 1148 688 677 455 245 239 232
20
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5272
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2572 2926 3182 4727 1132
Size: 97 1343 354 256 1545 2229
Fragments arranged by size:
2229 1545 1343 354 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PmlI CAC'GTG
Cuts at: 2263 2779 2263
Size: 516 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 2695 2176
Size: 519 5305
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2950 3003 2950
Size: 53 5771
PstI C_TGCA'G
Cuts at: 830 2421 3176 830
Size: 1591 755 3478
Fragments arranged by size:
3478 1591 755
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
SacI G_AGCT'C
Cuts at: 2739 2739
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SapI GCTCTTCn'nnn_
Cuts at: 2453 2453
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2417 3172 4307 4988 5179 826
Size: 219 1372 755 1135 681 191 1471
Fragments arranged by size:
1471 1372 1135 755 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2783 2930 3928 4829 5091 820
Size: 197 926 840 147 998 901 262 1553
Fragments arranged by size:
1553 998 926 901 840 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3121 3615 493
Size: 2628 494 2702
Fragments arranged by size:
2702 2628 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 2572 3182 1132
Size: 1440 610 3774
Fragments arranged by size:
3774 1440 610
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2493 2967 3263 513
Size: 552 1428 474 296 3074
Fragments arranged by size:
3074 1428 552 474 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 2703 4070
Size: 274 80 33 51 1443 444 282 1367
Cuts at: 4070 5782 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1367 444 282 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 2277 2309 5784 5816 2237
Size: 32 8 32 3475 32 2245
Fragments arranged by size:
3475 2245 32 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 2624 3953 2624
Size: 1329 4495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI