Restriction map for
pFN21ASDA1029
(Circular) (Six-base) MAPSORT of: pFN21ASDA1029.seq Check: 6386 from: 1 to: 7438
pFN21ASDA1029 7438 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2685 4781 1937
Size: 748 2096 4594
Fragments arranged by size:
4594 2096 748
Acc65I G'GTAC_C
Cuts at: 2092 3367 4759 2092
Size: 1275 1392 4771
Fragments arranged by size:
4771 1392 1275
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3104 5501 6885 7153 1632
Size: 1472 2397 1384 268 1917
Fragments arranged by size:
2397 1917 1472 1384 268
AfeI AGC'GCT
Cuts at: 4226 4226
Size: 7438
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 4599 6337 7314 1203
Size: 3396 1738 977 1327
Fragments arranged by size:
3396 1738 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 3070 6167 3070
Size: 3097 4341
AleI CACnn'nnGTG
Cuts at: 1166 2624 3929 1166
Size: 1458 1305 4675
Fragments arranged by size:
4675 1458 1305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3942 1184
Size: 2758 4680
AlwNI CAG_nnn'CTG
Cuts at: 2347 2411 5181 6753 2347
Size: 64 2770 1572 3032
Fragments arranged by size:
3032 2770 1572 64
ApaI G_GGCC'C
Cuts at: 4142 4142
Size: 7438
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 2371 4747 4973 5125 7234 1493
Size: 878 2376 226 152 2109 1697
Fragments arranged by size:
2376 2109 1697 878 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 3599 3990 4135 4763 1870
Size: 73 1656 391 145 628 4545
Fragments arranged by size:
4545 1656 628 391 145 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3359 3392 3359
Size: 33 7405
BamHI G'GATC_C
Cuts at: 2754 4768 5135 2754
Size: 2014 367 5057
Fragments arranged by size:
5057 2014 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 2092 2455 2761 2771
Size: 325 217 627 271 34 363 306 10
Cuts at: 2771 3367 3817 4349 4384 4759 6214 618
Size: 596 450 532 35 375 1455 1842
Fragments arranged by size:
1842 1455 627 596 532 450 375 363
325 306 271 217 35 34 10
BanII G_rGCy'C
Cuts at: 1391 2795 2867 3282 3471 3699 3841 3938 4136
Size: 1404 72 415 189 228 142 97 198
Cuts at: 4136 4142 4502 4544 4757 1391
Size: 6 360 42 213 4072
Fragments arranged by size:
4072 1404 415 360 228 213 198 189
142 97 72 42 6
BbeI G_GCGC'C
Cuts at: 2765 4353 4388 2765
Size: 1588 35 5815
Fragments arranged by size:
5815 1588 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3066 3507 928
Size: 971 1167 441 4859
Fragments arranged by size:
4859 1167 971 441
BbvCI CC'TCA_GC
Cuts at: 2202 2463 2202
Size: 261 7177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5629 5663 1903
Size: 34 3692 34 3678
Fragments arranged by size:
3692 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3063 5330 6540 3063
Size: 2267 1210 3961
Fragments arranged by size:
3961 2267 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3011 3602 4001
Size: 107 122 71 631 605 1339 591 399
Cuts at: 4001 4122 4561 6049 136
Size: 121 439 1488 1525
Fragments arranged by size:
1525 1488 1339 631 605 591 439 399
122 121 107 71
BglII A'GATC_T
Cuts at: 7433 7433
Size: 7438
BlpI GC'TnA_GC
Cuts at: 3465 4855 3465
Size: 1390 6048
Bme1580I G_kGCm'C
Cuts at: 1790 2774 3822 4142 5499 6655 1790
Size: 984 1048 320 1357 1156 2573
Fragments arranged by size:
2573 1357 1156 1048 984 320
BmgBI CAC'GTC
Cuts at: 1318 4600 1318
Size: 3282 4156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2244 2841 3299
Size: 416 53 83 399 24 827 597 458
Cuts at: 3299 5169 6127 442
Size: 1870 958 1753
Fragments arranged by size:
1870 1753 958 827 597 458 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2049 1056
Size: 993 6445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 3048 3397 3485 2050
Size: 998 349 88 6003
Fragments arranged by size:
6003 998 349 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2181 2493 2518 2545 3143 3958 4620
Size: 845 97 312 25 27 598 815 662
Cuts at: 4620 6098 1239
Size: 1478 2579
Fragments arranged by size:
2579 1478 845 815 662 598 312 97
27 25
Bpu10I CC'TnA_GC
Cuts at: 2202 2463 4047 2202
Size: 261 1584 5593
Fragments arranged by size:
5593 1584 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3374 4676 5563 6428 6726 3374
Size: 1302 887 865 298 4086
Fragments arranged by size:
4086 1302 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 3033 3775 3990 4244 6101 882
Size: 617 1485 49 742 215 254 1857 2219
Fragments arranged by size:
2219 1857 1485 742 617 254 215 49
BsaAI yAC'GTr
Cuts at: 493 1204 4735 5229 493
Size: 711 3531 494 2702
Fragments arranged by size:
3531 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2310 7432 2310
Size: 5122 2316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2762 2950 4088 4350
Size: 53 83 186 936 1229 188 1138 262
Cuts at: 4350 4385 5245 5627 6262 275
Size: 35 860 382 635 1451
Fragments arranged by size:
1451 1229 1138 936 860 635 382 262
188 186 83 53 35
BsaWI w'CCGG_w
Cuts at: 5871 6269 6543 6690 7101 5871
Size: 398 274 147 411 6208
Fragments arranged by size:
6208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7213
Fragments arranged by size:
7213 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2096 2480 2803 3021 3140 3287 3290
Size: 868 42 384 323 218 119 147 3
Cuts at: 3290 3374 3868 1186
Size: 84 494 4756
Fragments arranged by size:
4756 868 494 384 323 218 147 119
84 42 3
BseYI C'CCAG_C
Cuts at: 2172 2215 2423 2555 2569 2838 3056 3628 3800
Size: 43 208 132 14 269 218 572 172
Cuts at: 3800 4109 4125 4268 6641 7137 2172
Size: 309 16 143 2373 496 2473
Fragments arranged by size:
2473 2373 572 496 309 269 218 208
172 143 132 43 16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2955 4510 4708 1163
Size: 573 396 823 1555 198 3893
Fragments arranged by size:
3893 1555 823 573 396 198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4343 4888 5649 6677 1540
Size: 275 186 2342 545 761 1028 2301
Fragments arranged by size:
2342 2301 1028 761 545 275 186
BsiHKAI G_wGCw'C
Cuts at: 3471 5499 5584 6655 3471
Size: 2028 85 1071 4254
Fragments arranged by size:
4254 2028 1071 85
BsmI GAATG_Cn'
Cuts at: 2125 3117 4949 5042 2125
Size: 992 1832 93 4521
Fragments arranged by size:
4521 1832 992 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2800 2941 2800
Size: 141 7297
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2774 2795 2867 3282 3471 3699 3822
Size: 399 984 21 72 415 189 228 123
Cuts at: 3822 3841 3938 4136 4142 4502 4544 4757 5499
Size: 19 97 198 6 360 42 213 742
Cuts at: 5499 5584 6655 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 984 742 415 399 360 228
213 198 189 123 97 85 72 42
21 19 6
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3675 3834 4793 844
Size: 2831 159 959 3489
Fragments arranged by size:
3489 2831 959 159
BsrBI CCG'CTC
Cuts at: 1394 1823 4581 5325 1394
Size: 429 2758 744 3507
Fragments arranged by size:
3507 2758 744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5933 6107 65
Size: 1183 4685 174 1396
Fragments arranged by size:
4685 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2185 2323 2458 4511 6082 6269 1700
Size: 110 375 138 135 2053 1571 187 2869
Fragments arranged by size:
2869 2053 1571 375 187 138 135 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 3330 4213 5498 6510 3330
Size: 883 1285 1012 4258
Fragments arranged by size:
4258 1285 1012 883
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7438
BstBI TT'CG_AA
Cuts at: 2007 6242 2007
Size: 4235 3203
BstEII G'GTnAC_C
Cuts at: 1184 3187 1184
Size: 2003 5435
BstXI CCAn_nnnn'nTGG
Cuts at: 2782 3808 2782
Size: 1026 6412
BstYI r'GATC_y
Cuts at: 1972 2754 3211 4768 5135 5520 5537 6989 7075
Size: 782 457 1557 367 385 17 1452 86
Cuts at: 7075 7087 7433 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1557 1452 782 457 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2901 3348 3658 4142 4337 4361
Size: 552 957 879 447 310 484 195 24
Cuts at: 4361 4493 4587 4666 513
Size: 132 94 79 3285
Fragments arranged by size:
3285 957 879 552 484 447 310 195
132 94 79 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6699
Fragments arranged by size:
6699 683 56
BtsI GCAGTG_nn'
Cuts at: 2126 2967 3924 4954 5748 5768 2126
Size: 841 957 1030 794 20 3796
Fragments arranged by size:
3796 1030 957 841 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7403
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DraIII CAC_nnn'GTG
Cuts at: 2433 3332 2433
Size: 899 6539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6445 809
Size: 507 5129 1802
Fragments arranged by size:
5129 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2709 2739
Size: 54 1425 50 275 18 243 636 30
Cuts at: 2739 2870 3904 4885 5774 7403 8
Size: 131 1034 981 889 1629 43
Fragments arranged by size:
1629 1425 1034 981 889 636 275 243
131 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 5368 1353
Size: 729 3286 3423
Fragments arranged by size:
3423 3286 729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2283 3371 6025 6399 6545 387
Size: 1064 138 694 1088 2654 374 146 1280
Fragments arranged by size:
2654 1280 1088 1064 694 374 146 138
EcoICRI GAG'CTC
Cuts at: 3469 3469
Size: 7438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2181 2493 2518 2545 3104 3143
Size: 393 452 97 312 25 27 559 39
Cuts at: 3143 3958 4620 5501 6098 6885 7153 1239
Size: 815 662 881 597 787 268 1524
Fragments arranged by size:
1524 881 815 787 662 597 559 452
393 312 268 97 39 27 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 3022 3443 3830 2066
Size: 956 421 387 5674
Fragments arranged by size:
5674 956 421 387
EcoO109I rG'GnC_Cy
Cuts at: 2408 2565 2827 3603 3682 4003 4011 4563 2408
Size: 157 262 776 79 321 8 552 5283
Fragments arranged by size:
5283 776 552 321 262 157 79 8
EcoRI G'AATT_C
Cuts at: 2371 4747 5125 2371
Size: 2376 378 4684
Fragments arranged by size:
4684 2376 378
FspI TGC'GCA
Cuts at: 5944 5944
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 2765 4228 4289 4332 4353 4388 6585 1678
Size: 1087 1463 61 43 21 35 2197 2531
Fragments arranged by size:
2531 2197 1463 1087 61 43 35 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2486 2518 2570 2602 2981
Size: 32 135 32 914 32 52 32 379
Cuts at: 2981 3013 3056 3088 3181 3213 4030 4062 6079
Size: 32 43 32 93 32 817 32 2017
Cuts at: 6079 6111 6153 6185 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2017 914 817 379 135 93 52
43 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2230 2731 4782 5028 669
Size: 988 281 292 501 2051 246 3079
Fragments arranged by size:
3079 2051 988 501 292 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7438
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KasI G'GCGC_C
Cuts at: 2761 4349 4384 2761
Size: 1588 35 5815
Fragments arranged by size:
5815 1588 35
KpnI G_GTAC'C
Cuts at: 2096 3371 4763 2096
Size: 1275 1392 4771
Fragments arranged by size:
4771 1392 1275
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2389 3087 3330 4620 6552 6736 1305
Size: 1084 698 243 1290 1932 184 2007
Fragments arranged by size:
2007 1932 1290 1084 698 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2711 10
Size: 54 1425 343 243 636 4737
Fragments arranged by size:
4737 1425 636 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 3929 5396 5755 5914 518
Size: 648 1458 1305 1467 359 159 2042
Fragments arranged by size:
2042 1467 1458 1305 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2201 2219 2663 3227 3350 3543 3721 4186
Size: 595 18 444 564 123 193 178 465
Cuts at: 4186 4240 4279 4339 4363 4495 4546 4668 4854
Size: 54 39 60 24 132 51 122 186
Cuts at: 4854 5531 6679 6924 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 564 465 444 245
193 186 178 132 123 122 60 54
51 39 24 18
NarI GG'CG_CC
Cuts at: 2762 4350 4385 2762
Size: 1588 35 5815
Fragments arranged by size:
5815 1588 35
NcoI C'CATG_G
Cuts at: 513 1065 2022 2901 3658 513
Size: 552 957 879 757 4293
Fragments arranged by size:
4293 957 879 757 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NheI G'CTAG_C
Cuts at: 1052 2045 1052
Size: 993 6445
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 4429 4796 6341 1132
Size: 97 3200 367 1545 2229
Fragments arranged by size:
3200 2229 1545 367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7438
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2399 3981 1108
Size: 1291 1582 4565
Fragments arranged by size:
4565 1582 1291
PfoI T'CCnGG_A
Cuts at: 3575 3575
Size: 7438
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3682 3682
Size: 7438
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PspOMI G'GGCC_C
Cuts at: 4138 4138
Size: 7438
PstI C_TGCA'G
Cuts at: 830 2224 3921 4284 4790 830
Size: 1394 1697 363 506 3478
Fragments arranged by size:
3478 1697 1394 506 363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 2201 2219 2663 3721 4279 2201
Size: 18 444 1058 558 5360
Fragments arranged by size:
5360 1058 558 444 18
SacI G_AGCT'C
Cuts at: 3471 3471
Size: 7438
SacII CC_GC'GG
Cuts at: 3351 4340 4364 4496 4669 3351
Size: 989 24 132 173 6120
Fragments arranged by size:
6120 989 173 132 24
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SexAI A'CCwGG_T
Cuts at: 3813 3813
Size: 7438
SfcI C'TryA_G
Cuts at: 826 1045 2220 2686 3917 4280 4786 5921 6602
Size: 219 1175 466 1231 363 506 1135 681
Cuts at: 6602 6793 826
Size: 191 1471
Fragments arranged by size:
1471 1231 1175 1135 681 506 466 363
219 191
SfoI GGC'GCC
Cuts at: 2763 4351 4386 2763
Size: 1588 35 5815
Fragments arranged by size:
5815 1588 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 3601 4137 4765 1872
Size: 1729 536 628 4545
Fragments arranged by size:
4545 1729 628 536
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3389 4691 5542 6443 6705 820
Size: 197 926 1446 1302 851 901 262 1553
Fragments arranged by size:
1553 1446 1302 926 901 851 262 197
SnaBI TAC'GTA
Cuts at: 493 4735 5229 493
Size: 4242 494 2702
Fragments arranged by size:
4242 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4429 4796 1132
Size: 3297 367 3774
Fragments arranged by size:
3774 3297 367
SrfI GCCC'GGGC
Cuts at: 4137 4137
Size: 7438
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 2746 3302 3449 4718 1517
Size: 1229 556 147 1269 4237
Fragments arranged by size:
4237 1269 1229 556 147
StyI C'CwwG_G
Cuts at: 513 1065 2022 2679 2901 2955 3183 3658 3765
Size: 552 957 657 222 54 228 475 107
Cuts at: 3765 3807 3831 4877 513
Size: 42 24 1046 3074
Fragments arranged by size:
3074 1046 957 657 552 475 228 222
107 54 42 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3010 4117 4268 5465 5482 5635 5820
Size: 1255 1430 1107 151 1197 17 153 185
Cuts at: 5820 6265 325
Size: 445 1498
Fragments arranged by size:
1498 1430 1255 1197 1107 445 185 153
151 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5684 7396 96
Size: 274 80 33 51 1443 3707 1712 138
Fragments arranged by size:
3707 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 3387 3419 7398 7430 2873
Size: 32 482 32 3979 32 2881
Fragments arranged by size:
3979 2881 482 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2995 1316
Size: 1679 5759
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2623 3007 2623
Size: 384 7054
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 3599 4135 4763 1870
Size: 1729 536 628 4545
Fragments arranged by size:
4545 1729 628 536
XmnI GAAnn'nnTTC
Cuts at: 5567 5567
Size: 7438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SanDI SapI SfiI
SwaI