Restriction map for
pFN21ASDA1014
(Circular) (Six-base) MAPSORT of: pFN21ASDA1014.seq Check: 9325 from: 1 to: 7780
pFN21ASDA1014 7780 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4636 4636
Size: 7780
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5590 278
Size: 53 83 186 936 4054 2468
Fragments arranged by size:
4054 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5123 1937
Size: 3186 4594
Acc65I G'GTAC_C
Cuts at: 2044 5101 2044
Size: 3057 4723
AclI AA'CG_TT
Cuts at: 1563 5907 6280 1563
Size: 4344 373 3063
Fragments arranged by size:
4344 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 3752 4094 4296 5066 5843 7227 7495
Size: 582 1538 342 202 770 777 1384 268
Cuts at: 7495 1632
Size: 1917
Fragments arranged by size:
1917 1538 1384 777 770 582 342 268
202
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7583
AflIII A'CryG_T
Cuts at: 1203 6679 7656 1203
Size: 5476 977 1327
Fragments arranged by size:
5476 1327 977
AgeI A'CCGG_T
Cuts at: 6611 6611
Size: 7780
AhdI GACnn_n'nnGTC
Cuts at: 6509 6509
Size: 7780
AleI CACnn'nnGTG
Cuts at: 1166 2195 4418 1166
Size: 1029 2223 4528
Fragments arranged by size:
4528 2223 1029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7780
AlwNI CAG_nnn'CTG
Cuts at: 2510 2822 4628 4978 5523 7095 2510
Size: 312 1806 350 545 1572 3195
Fragments arranged by size:
3195 1806 1572 545 350 312
ApaLI G'TGCA_C
Cuts at: 5837 6993 5837
Size: 1156 6624
ApoI r'AATT_y
Cuts at: 1493 2943 3493 3514 3602 3641 4000 5089 5315
Size: 1450 550 21 88 39 359 1089 226
Cuts at: 5315 5467 7576 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1450 1089 550 359 226 152
88 39 21
AseI AT'TA_AT
Cuts at: 160 1032 3660 6334 160
Size: 872 2628 2674 1606
Fragments arranged by size:
2674 2628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7780
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2088 4701 5105 1870
Size: 73 94 51 2613 404 4545
Fragments arranged by size:
4545 2613 404 94 73 51
BamHI G'GATC_C
Cuts at: 5110 5477 5110
Size: 367 7413
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 5101 6556 618
Size: 325 217 627 257 3057 1455 1842
Fragments arranged by size:
3057 1842 1455 627 325 257 217
BanII G_rGCy'C
Cuts at: 1391 3739 4272 5099 1391
Size: 2348 533 827 4072
Fragments arranged by size:
4072 2348 827 533
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3168 928
Size: 971 1269 5540
Fragments arranged by size:
5540 1269 971
BbvCI CC'TCA_GC
Cuts at: 2496 2496
Size: 7780
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5971 6005 1903
Size: 34 4034 34 3678
Fragments arranged by size:
4034 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4889 5672 6882 4889
Size: 783 1210 5787
Fragments arranged by size:
5787 1210 783
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7780
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2177 2210 6391
Size: 107 122 71 631 605 505 33 4181
Cuts at: 6391 136
Size: 1525
Fragments arranged by size:
4181 1525 631 605 505 122 107 71
33
BglII A'GATC_T
Cuts at: 7775 7775
Size: 7780
BlpI GC'TnA_GC
Cuts at: 5197 5197
Size: 7780
Bme1580I G_kGCm'C
Cuts at: 1790 5841 6997 1790
Size: 4051 1156 2573
Fragments arranged by size:
4051 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7780
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2799 3844 5511
Size: 416 53 83 399 24 1382 1045 1667
Cuts at: 5511 6469 442
Size: 958 1753
Fragments arranged by size:
1753 1667 1382 1045 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7780
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 7780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3222 3263 3444 3531 4161 4437 6440 1239
Size: 1983 41 181 87 630 276 2003 2579
Fragments arranged by size:
2579 2003 1983 630 276 181 87 41
Bpu10I CC'TnA_GC
Cuts at: 2496 2496
Size: 7780
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3333 5905 6770 7068 3333
Size: 2572 865 298 4045
Fragments arranged by size:
4045 2572 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6443 882
Size: 617 4944 2219
Fragments arranged by size:
4944 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5077 5571 493
Size: 711 3873 494 2702
Fragments arranged by size:
3873 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7774 7774
Size: 7780
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5587 5969 6604 275
Size: 53 83 186 936 4054 382 635 1451
Fragments arranged by size:
4054 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6213 6611 6885 7032 7443 6213
Size: 398 274 147 411 6550
Fragments arranged by size:
6550 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3424 3454 1178
Size: 30 165 30 2021 30 5504
Fragments arranged by size:
5504 2021 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2484 2981 2984 3083 3227 4188 4200 4280
Size: 1298 497 3 99 144 961 12 80
Cuts at: 4280 4740 4743 4746 4749 4752 1186
Size: 460 3 3 3 3 4214
Fragments arranged by size:
4214 1298 961 497 460 144 99 80
12 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2159 6983 7479 2159
Size: 4824 496 2460
Fragments arranged by size:
4824 2460 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 4926 1163
Size: 573 543 2647 4017
Fragments arranged by size:
4017 2647 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2053 5230 5991 7019 1540
Size: 275 186 52 3177 761 1028 2301
Fragments arranged by size:
3177 2301 1028 761 275 186 52
BsiHKAI G_wGCw'C
Cuts at: 5841 5926 6997 5841
Size: 85 1071 6624
Fragments arranged by size:
6624 1071 85
BsmI GAATG_Cn'
Cuts at: 3363 5291 5384 3363
Size: 1928 93 5759
Fragments arranged by size:
5759 1928 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3739 4272 5099 5841 5926 6997 1391
Size: 399 1949 533 827 742 85 1071 2174
Fragments arranged by size:
2174 1949 1071 827 742 533 399 85
BspEI T'CCGG_A
Cuts at: 7443 7443
Size: 7780
BspHI T'CATG_A
Cuts at: 2301 5669 7399 2301
Size: 3368 1730 2682
Fragments arranged by size:
3368 2682 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4636 5135 844
Size: 3792 499 3489
Fragments arranged by size:
3792 3489 499
BsrBI CCG'CTC
Cuts at: 1394 1823 5667 1394
Size: 429 3844 3507
Fragments arranged by size:
3844 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 6275 6449 65
Size: 1183 3434 1593 174 1396
Fragments arranged by size:
3434 1593 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6424 6611 1700
Size: 110 4614 187 2869
Fragments arranged by size:
4614 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7780
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7780
BssSI C'ACGA_G
Cuts at: 5840 6852 5840
Size: 1012 6768
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3485 4628 794
Size: 2691 1143 3946
Fragments arranged by size:
3946 2691 1143
BstBI TT'CG_AA
Cuts at: 2007 6584 2007
Size: 4577 3203
BstEII G'GTnAC_C
Cuts at: 1184 4487 1184
Size: 3303 4477
BstXI CCAn_nnnn'nTGG
Cuts at: 2621 2621
Size: 7780
BstYI r'GATC_y
Cuts at: 1972 3178 4230 5110 5477 5862 5879 7331 7417
Size: 1206 1052 880 367 385 17 1452 86
Cuts at: 7417 7429 7775 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1206 1052 880 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3015 5025 3015
Size: 2010 5770
BtgI C'CryG_G
Cuts at: 513 1065 2022 2148 2193 2208 513
Size: 552 957 126 45 15 6085
Fragments arranged by size:
6085 957 552 126 45 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2976 487
Size: 683 56 1750 5291
Fragments arranged by size:
5291 1750 683 56
BtsI GCAGTG_nn'
Cuts at: 3193 4413 4425 5296 6090 6110 3193
Size: 1220 12 871 794 20 4863
Fragments arranged by size:
4863 1220 871 794 20 12
ClaI AT'CG_AT
Cuts at: 1554 2478 1554
Size: 924 6856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7745
DraI TTT'AAA
Cuts at: 5084 5084
Size: 7780
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6787 809
Size: 507 5471 1802
Fragments arranged by size:
5471 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2457 5227
Size: 54 1425 50 275 18 220 407 2770
Cuts at: 5227 6116 7745 8
Size: 889 1629 43
Fragments arranged by size:
2770 1629 1425 889 407 275 220 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2050 5227 1537
Size: 275 238 3177 4090
Fragments arranged by size:
4090 3177 275 238
EarI CTCTTCn'nnn_
Cuts at: 1353 3030 3213 3906 4829 4893 4917 4953 5042
Size: 1677 183 693 923 64 24 36 89
Cuts at: 5042 5710 1353
Size: 668 3423
Fragments arranged by size:
3423 1677 923 693 668 183 89 64
36 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2067 6367 6741 6887 387
Size: 1064 138 478 4300 374 146 1280
Fragments arranged by size:
4300 1280 1064 478 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 3222 3263 3444 3531 3752 4094
Size: 393 582 1008 41 181 87 221 342
Cuts at: 4094 4161 4296 4437 5066 5843 6440 7227 7495
Size: 67 135 141 629 777 597 787 268
Cuts at: 7495 1239
Size: 1524
Fragments arranged by size:
1524 1008 787 777 629 597 582 393
342 268 221 181 141 135 87 67
41
EcoNI CCTnn'n_nnAGG
Cuts at: 2232 4278 5024 2232
Size: 2046 746 4988
Fragments arranged by size:
4988 2046 746
EcoO109I rG'GnC_Cy
Cuts at: 2084 2110 2084
Size: 26 7754
EcoRI G'AATT_C
Cuts at: 2943 3514 5089 5467 2943
Size: 571 1575 378 5256
Fragments arranged by size:
5256 1575 571 378
EcoRV GAT'ATC
Cuts at: 3331 3331
Size: 7780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3251 3755 3251
Size: 504 7276
FspI TGC'GCA
Cuts at: 6286 6286
Size: 7780
HaeII r_GCGC'y
Cuts at: 1678 6927 1678
Size: 5249 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3657 3689 3694 3726 4919
Size: 32 135 32 2085 32 5 32 1193
Cuts at: 4919 4951 6421 6453 6495 6527 1373
Size: 32 1470 32 42 32 2626
Fragments arranged by size:
2626 2085 1470 1193 135 42 32 32
32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2436 5124 5370 669
Size: 988 281 498 2688 246 3079
Fragments arranged by size:
3079 2688 988 498 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7780
HpaI GTT'AAC
Cuts at: 5370 5370
Size: 7780
KpnI G_GTAC'C
Cuts at: 2048 5105 2048
Size: 3057 4723
MfeI C'AATT_G
Cuts at: 3480 4662 5379 3480
Size: 1182 717 5881
Fragments arranged by size:
5881 1182 717
MluI A'CGCG_T
Cuts at: 7656 7656
Size: 7780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2558 2641 3129 3702 6894 7078 1305
Size: 1253 83 488 573 3192 184 2007
Fragments arranged by size:
3192 2007 1253 573 488 184 83
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 10
Size: 54 1425 343 627 5331
Fragments arranged by size:
5331 1425 627 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 4418 5738 6097 6256 518
Size: 648 1029 2223 1320 359 159 2042
Fragments arranged by size:
2223 2042 1320 1029 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2150 2195 2210 2321 4978 5196 5873 7021
Size: 544 45 15 111 2657 218 677 1148
Cuts at: 7021 7266 1606
Size: 245 2120
Fragments arranged by size:
2657 2120 1148 677 544 245 218 111
45 15
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6271
Fragments arranged by size:
6271 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7780
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7780
NotI GC'GGCC_GC
Cuts at: 5227 5227
Size: 7780
NspI r_CATG'y
Cuts at: 1132 1229 5138 6683 1132
Size: 97 3909 1545 2229
Fragments arranged by size:
3909 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7780
PciI A'CATG_T
Cuts at: 6679 6679
Size: 7780
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3467 1108
Size: 2359 5421
PfoI T'CCnGG_A
Cuts at: 4233 4233
Size: 7780
PmeI GTTT'AAAC
Cuts at: 5084 5084
Size: 7780
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5849 5881 7362 7394 5849
Size: 32 1481 32 6235
Fragments arranged by size:
6235 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2084 2110 2084
Size: 26 7754
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7780
PsiI TTA'TAA
Cuts at: 5350 5350
Size: 7780
PstI C_TGCA'G
Cuts at: 830 2506 4420 4599 5132 830
Size: 1676 1914 179 533 3478
Fragments arranged by size:
3478 1914 1676 533 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7780
PvuII CAG'CTG
Cuts at: 2321 4978 2321
Size: 2657 5123
SacII CC_GC'GG
Cuts at: 2151 2196 2211 2151
Size: 45 15 7720
Fragments arranged by size:
7720 45 15
SalI G'TCGA_C
Cuts at: 1936 5122 1936
Size: 3186 4594
SanDI GG'GwC_CC
Cuts at: 2110 2110
Size: 7780
SbfI CC_TGCA'GG
Cuts at: 5132 5132
Size: 7780
ScaI AGT'ACT
Cuts at: 6028 7740 6028
Size: 1712 6068
SfcI C'TryA_G
Cuts at: 826 1045 2502 2511 4401 4416 4462 4476 4482
Size: 219 1457 9 1890 15 46 14 6
Cuts at: 4482 4595 5128 6263 6944 7135 826
Size: 113 533 1135 681 191 1471
Fragments arranged by size:
1890 1471 1457 1135 681 533 219 191
113 46 15 14 9 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7780
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7780
SmaI CCC'GGG
Cuts at: 1872 2039 5107 1872
Size: 167 3068 4545
Fragments arranged by size:
4545 3068 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3348 5884 6785 7047 820
Size: 197 926 1405 2536 901 262 1553
Fragments arranged by size:
2536 1553 1405 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5077 5571 493
Size: 4584 494 2702
Fragments arranged by size:
4584 2702 494
SpeI A'CTAG_T
Cuts at: 152 2828 152
Size: 2676 5104
SphI G_CATG'C
Cuts at: 1132 5138 1132
Size: 4006 3774
SspI AAT'ATT
Cuts at: 5 52 5704 5
Size: 47 5652 2081
Fragments arranged by size:
5652 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7780
StyI C'CwwG_G
Cuts at: 513 1065 2022 3282 4146 4609 5219 513
Size: 552 957 1260 864 463 610 3074
Fragments arranged by size:
3074 1260 957 864 610 552 463
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2627 5807 5824 5977 6162 6607 325
Size: 1255 1047 3180 17 153 185 445 1498
Fragments arranged by size:
3180 1498 1255 1047 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2450 3098 3562
Size: 274 80 33 51 1443 473 648 464
Cuts at: 3562 6026 7738 96
Size: 2464 1712 138
Fragments arranged by size:
2464 1712 1443 648 473 464 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7740 7772 7740
Size: 32 7748
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7780
XbaI T'CTAG_A
Cuts at: 5116 5116
Size: 7780
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7780
XmaI C'CCGG_G
Cuts at: 1870 2037 5105 1870
Size: 167 3068 4545
Fragments arranged by size:
4545 3068 167
XmnI GAAnn'nnTTC
Cuts at: 2943 5019 5909 2943
Size: 2076 890 4814
Fragments arranged by size:
4814 2076 890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5588 276
Size: 53 83 186 936 4054 2468
Fragments arranged by size:
4054 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI
SrfI SwaI XcmI