Restriction map for
pFN21ASDA1004
(Circular) (Six-base) MAPSORT of: pFN21ASDA1004.seq Check: 4673 from: 1 to: 8215
pFN21ASDA1004 8215 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6025 278
Size: 53 83 186 936 4489 2468
Fragments arranged by size:
4489 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 2882 5558 1937
Size: 592 353 2676 4594
Fragments arranged by size:
4594 2676 592 353
Acc65I G'GTAC_C
Cuts at: 2069 2672 5536 2069
Size: 603 2864 4748
Fragments arranged by size:
4748 2864 603
AclI AA'CG_TT
Cuts at: 1563 6342 6715 1563
Size: 4779 373 3063
Fragments arranged by size:
4779 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4404 6278 7662 7930 1632
Size: 1661 1111 1874 1384 268 1917
Fragments arranged by size:
1917 1874 1661 1384 1111 268
AfeI AGC'GCT
Cuts at: 3038 4289 3038
Size: 1251 6964
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8018
AflIII A'CryG_T
Cuts at: 1203 2535 3878 7114 8091 1203
Size: 1332 1343 3236 977 1327
Fragments arranged by size:
3236 1343 1332 1327 977
AgeI A'CCGG_T
Cuts at: 7046 7046
Size: 8215
AhdI GACnn_n'nnGTC
Cuts at: 6944 6944
Size: 8215
AleI CACnn'nnGTG
Cuts at: 1166 2900 4269 1166
Size: 1734 1369 5112
Fragments arranged by size:
5112 1734 1369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2936 1184
Size: 1752 6463
AlwNI CAG_nnn'CTG
Cuts at: 3689 4309 5000 5241 5356 5958 7530 3689
Size: 620 691 241 115 602 1572 4374
Fragments arranged by size:
4374 1572 691 620 602 241 115
ApaLI G'TGCA_C
Cuts at: 6272 7428 6272
Size: 1156 7059
ApoI r'AATT_y
Cuts at: 1493 2260 3080 3590 5076 5524 5750 5902 8011
Size: 767 820 510 1486 448 226 152 2109
Cuts at: 8011 1493
Size: 1697
Fragments arranged by size:
2109 1697 1486 820 767 510 448 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6769 160
Size: 872 5737 1606
Fragments arranged by size:
5737 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8215
AvaI C'yCGr_G
Cuts at: 1870 1943 2459 4218 5540 1870
Size: 73 516 1759 1322 4545
Fragments arranged by size:
4545 1759 1322 516 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2664 2697 2664
Size: 33 8182
BamHI G'GATC_C
Cuts at: 2290 3340 5545 5912 2290
Size: 1050 2205 367 4593
Fragments arranged by size:
4593 2205 1050 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2672 3853 5536 6991
Size: 325 217 627 282 603 1181 1683 1455
Cuts at: 6991 618
Size: 1842
Fragments arranged by size:
1842 1683 1455 1181 627 603 325 282
217
BanII G_rGCy'C
Cuts at: 1391 2832 3320 4452 4678 5319 5534 1391
Size: 1441 488 1132 226 641 215 4072
Fragments arranged by size:
4072 1441 1132 641 488 226 215
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2704 3254 4717 5283 928
Size: 971 805 550 1463 566 3860
Fragments arranged by size:
3860 1463 971 805 566 550
BbvCI CC'TCA_GC
Cuts at: 3098 4736 5006 3098
Size: 1638 270 6307
Fragments arranged by size:
6307 1638 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5402 5412 5436 5446 6406 6440 1903
Size: 34 3465 10 24 10 960 34 3678
Fragments arranged by size:
3678 3465 960 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 6107 7317 6107
Size: 1210 7005
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4490 6826 136
Size: 107 122 71 631 605 2818 2336 1525
Fragments arranged by size:
2818 2336 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8210 8210
Size: 8215
BlpI GC'TnA_GC
Cuts at: 5632 5632
Size: 8215
Bme1580I G_kGCm'C
Cuts at: 1790 4473 4851 6276 7432 1790
Size: 2683 378 1425 1156 2573
Fragments arranged by size:
2683 2573 1425 1156 378
BmgBI CAC'GTC
Cuts at: 1318 2359 4779 1318
Size: 1041 2420 4754
Fragments arranged by size:
4754 2420 1041
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2533 3251 3340
Size: 416 53 83 399 24 1116 718 89
Cuts at: 3340 5946 6904 442
Size: 2606 958 1753
Fragments arranged by size:
2606 1753 1116 958 718 416 399 89
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2262 2456 4078 2262
Size: 194 1622 6399
Fragments arranged by size:
6399 1622 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2765 3530 3570 3968 4999 6875 1239
Size: 1262 264 765 40 398 1031 1876 2579
Fragments arranged by size:
2579 1876 1262 1031 765 398 264 40
Bpu10I CC'TnA_GC
Cuts at: 3098 4677 4736 5006 3098
Size: 1579 59 270 6307
Fragments arranged by size:
6307 1579 270 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2257 2816 3196 4846 6340 7205 7503 2257
Size: 559 380 1650 1494 865 298 2969
Fragments arranged by size:
2969 1650 1494 865 559 380 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 6878 882
Size: 617 909 4470 2219
Fragments arranged by size:
4470 2219 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 5512 6006 493
Size: 711 4308 494 2702
Fragments arranged by size:
4308 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8209 8209
Size: 8215
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6022 6404 7039 275
Size: 53 83 186 936 4489 382 635 1451
Fragments arranged by size:
4489 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5136 6648 7046 7320 7467 7878 5136
Size: 1512 398 274 147 411 5473
Fragments arranged by size:
5473 1512 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2582 2612 1178
Size: 30 165 30 1179 30 6781
Fragments arranged by size:
6781 1179 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 2441 3527 4486 4586 4604 4607 4616
Size: 1252 3 1086 959 100 18 3 9
Cuts at: 4616 4619 4622 4625 4628 4649 4958 1186
Size: 3 3 3 3 21 309 4443
Fragments arranged by size:
4443 1252 1086 959 309 100 21 18
9 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3287 4029 4256 4361 4543 4557 4579 7418 7914
Size: 742 227 105 182 14 22 2839 496
Cuts at: 7914 3287
Size: 3588
Fragments arranged by size:
3588 2839 742 496 227 182 105 22
14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2513 2630 3425 4553 4650 4665
Size: 573 402 375 117 795 1128 97 15
Cuts at: 4665 1163
Size: 4713
Fragments arranged by size:
4713 1128 795 573 402 375 117 97
15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5665 6426 7454 1540
Size: 275 186 3664 761 1028 2301
Fragments arranged by size:
3664 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2832 3355 4452 5319 5439 6276 6361 7432 2832
Size: 523 1097 867 120 837 85 1071 3615
Fragments arranged by size:
3615 1097 1071 867 837 523 120 85
BsmI GAATG_Cn'
Cuts at: 3950 5726 5819 3950
Size: 1776 93 6346
Fragments arranged by size:
6346 1776 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3715 4680 3715
Size: 965 7250
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2832 3320 3355 4452 4473 4678 4851
Size: 399 1042 488 35 1097 21 205 173
Cuts at: 4851 5319 5439 5534 6276 6361 7432 1391
Size: 468 120 95 742 85 1071 2174
Fragments arranged by size:
2174 1097 1071 1042 742 488 468 399
205 173 120 95 85 35 21
BspEI T'CCGG_A
Cuts at: 5136 7878 5136
Size: 2742 5473
BspHI T'CATG_A
Cuts at: 6104 7834 6104
Size: 1730 6485
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 5318 5570 844
Size: 2416 2058 252 3489
Fragments arranged by size:
3489 2416 2058 252
BsrBI CCG'CTC
Cuts at: 1394 1823 6102 1394
Size: 429 4279 3507
Fragments arranged by size:
4279 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3486 3964 5373 6710 6884 65
Size: 1183 2238 478 1409 1337 174 1396
Fragments arranged by size:
2238 1409 1396 1337 1183 478 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5098 6859 7046 1700
Size: 110 3288 1761 187 2869
Fragments arranged by size:
3288 2869 1761 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8215
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8215
BssSI C'ACGA_G
Cuts at: 6275 7287 6275
Size: 1012 7203
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3252 4309 5000 794
Size: 2458 1057 691 4009
Fragments arranged by size:
4009 2458 1057 691
BstBI TT'CG_AA
Cuts at: 2007 7019 2007
Size: 5012 3203
BstEII G'GTnAC_C
Cuts at: 1184 2798 1184
Size: 1614 6601
BstXI CCAn_nnnn'nTGG
Cuts at: 4029 4029
Size: 8215
BstYI r'GATC_y
Cuts at: 1972 2242 2290 3340 4401 5545 5912 6297 6314
Size: 270 48 1050 1061 1144 367 385 17
Cuts at: 6314 7766 7852 7864 8210 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1144 1061 1050 385 367 346
270 86 48 17 12
BstZ17I GTA'TAC
Cuts at: 2883 2883
Size: 8215
Bsu36I CC'TnA_GG
Cuts at: 2860 2860
Size: 8215
BtgI C'CryG_G
Cuts at: 513 1065 2022 2388 2505 3662 4335 4453 513
Size: 552 957 366 117 1157 673 118 4275
Fragments arranged by size:
4275 1157 957 673 552 366 118 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3745 4161 4296 487
Size: 683 56 2519 416 135 4406
Fragments arranged by size:
4406 2519 683 416 135 56
BtsI GCAGTG_nn'
Cuts at: 3159 3437 4537 5239 5277 5731 6525 6545 3159
Size: 278 1100 702 38 454 794 20 4829
Fragments arranged by size:
4829 1100 794 702 454 278 38 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8180
DraI TTT'AAA
Cuts at: 5519 5519
Size: 8215
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7222 809
Size: 507 5906 1802
Fragments arranged by size:
5906 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2540 3569 3602
Size: 54 1425 50 275 18 710 1029 33
Cuts at: 3602 4660 5662 6551 8180 8
Size: 1058 1002 889 1629 43
Fragments arranged by size:
1629 1425 1058 1029 1002 889 710 275
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 5662 1537
Size: 275 3850 4090
Fragments arranged by size:
4090 3850 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 3906 4086 4593 6145 1353
Size: 786 1767 180 507 1552 3423
Fragments arranged by size:
3423 1767 1552 786 507 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6802 7176 7322 387
Size: 1064 138 5213 374 146 1280
Fragments arranged by size:
5213 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2830 4450 5317 2830
Size: 1620 867 5728
Fragments arranged by size:
5728 1620 867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2765 3293 3530 3570 3968 4404
Size: 393 869 264 528 237 40 398 436
Cuts at: 4404 4999 6278 6875 7662 7930 1239
Size: 595 1279 597 787 268 1524
Fragments arranged by size:
1524 1279 869 787 597 595 528 436
398 393 268 264 237 40
EcoNI CCTnn'n_nnAGG
Cuts at: 3074 3074
Size: 8215
EcoO109I rG'GnC_Cy
Cuts at: 2499 3809 4855 5034 5068 2499
Size: 1310 1046 179 34 5646
Fragments arranged by size:
5646 1310 1046 179 34
EcoRI G'AATT_C
Cuts at: 2260 5524 5902 2260
Size: 3264 378 4573
Fragments arranged by size:
4573 3264 378
FspI TGC'GCA
Cuts at: 6721 6721
Size: 8215
HaeII r_GCGC'y
Cuts at: 1678 3040 4291 7362 1678
Size: 1362 1251 3071 2531
Fragments arranged by size:
3071 2531 1362 1251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 2792 2824 3955
Size: 32 135 32 805 32 383 32 1131
Cuts at: 3955 3987 4401 4433 5026 5058 5217 5249 5281
Size: 32 414 32 593 32 159 32 32
Cuts at: 5281 6856 6888 6930 6962 1373
Size: 1575 32 42 32 2626
Fragments arranged by size:
2626 1575 1131 805 593 414 383 159
135 42 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3223 3669 5559 5805 669
Size: 988 281 1285 446 1890 246 3079
Fragments arranged by size:
3079 1890 1285 988 446 281 246
HindIII A'AGCT_T
Cuts at: 748 3584 748
Size: 2836 5379
HpaI GTT'AAC
Cuts at: 3669 5805 3669
Size: 2136 6079
KpnI G_GTAC'C
Cuts at: 2073 2676 5540 2073
Size: 603 2864 4748
Fragments arranged by size:
4748 2864 603
MfeI C'AATT_G
Cuts at: 5814 5814
Size: 8215
MluI A'CGCG_T
Cuts at: 8091 8091
Size: 8215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3012 3105 3405 3865 4883 7329 7513 1305
Size: 1707 93 300 460 1018 2446 184 2007
Fragments arranged by size:
2446 2007 1707 1018 460 300 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2542 3571 10
Size: 54 1425 343 710 1029 4654
Fragments arranged by size:
4654 1425 1029 710 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2381 2900 2939 4184 4269 6173
Size: 648 1191 24 519 39 1245 85 1904
Cuts at: 6173 6532 6691 518
Size: 359 159 2042
Fragments arranged by size:
2042 1904 1245 1191 648 519 359 159
85 39 24
MspA1I CmG'CkG
Cuts at: 1606 2227 3686 4365 4705 5019 5631 6308 7456
Size: 621 1459 679 340 314 612 677 1148
Cuts at: 7456 7701 1606
Size: 245 2120
Fragments arranged by size:
2120 1459 1148 679 677 621 612 340
314 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2388 4335 513
Size: 552 957 366 1947 4393
Fragments arranged by size:
4393 1947 957 552 366
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8215
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8215
NotI GC'GGCC_GC
Cuts at: 5662 5662
Size: 8215
NspI r_CATG'y
Cuts at: 1132 1229 2539 3882 5212 5573 7118 1132
Size: 97 1310 1343 1330 361 1545 2229
Fragments arranged by size:
2229 1545 1343 1330 1310 361 97
PasI CC'CwG_GG
Cuts at: 1348 4244 1348
Size: 2896 5319
PciI A'CATG_T
Cuts at: 2535 3878 7114 2535
Size: 1343 3236 3636
Fragments arranged by size:
3636 3236 1343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2743 1108
Size: 1635 6580
PmeI GTTT'AAAC
Cuts at: 5519 5519
Size: 8215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6284 6316 7797 7829 6284
Size: 32 1481 32 6670
Fragments arranged by size:
6670 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5034 5068 5034
Size: 34 8181
PshAI GACnn'nnGTC
Cuts at: 1613 5235 1613
Size: 3622 4593
PsiI TTA'TAA
Cuts at: 5785 5785
Size: 8215
PspXI vC'TCGA_Gb
Cuts at: 2459 2459
Size: 8215
PstI C_TGCA'G
Cuts at: 830 3788 4194 4320 4482 4996 5246 5567 830
Size: 2958 406 126 162 514 250 321 3478
Fragments arranged by size:
3478 2958 514 406 321 250 162 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8215
PvuII CAG'CTG
Cuts at: 3686 5019 3686
Size: 1333 6882
SacI G_AGCT'C
Cuts at: 2832 4452 5319 2832
Size: 1620 867 5728
Fragments arranged by size:
5728 1620 867
SalI G'TCGA_C
Cuts at: 1936 5557 1936
Size: 3621 4594
SbfI CC_TGCA'GG
Cuts at: 5567 5567
Size: 8215
ScaI AGT'ACT
Cuts at: 2618 6463 8175 2618
Size: 3845 1712 2658
Fragments arranged by size:
3845 2658 1712
SexAI A'CCwGG_T
Cuts at: 5110 5110
Size: 8215
SfcI C'TryA_G
Cuts at: 826 1045 2730 2889 3207 3307 3558 3784 4190
Size: 219 1685 159 318 100 251 226 406
Cuts at: 4190 4316 4430 4478 4992 5242 5563 6698 7379
Size: 126 114 48 514 250 321 1135 681
Cuts at: 7379 7570 826
Size: 191 1471
Fragments arranged by size:
1685 1471 1135 681 514 406 321 318
251 250 226 219 191 159 126 114
100 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 4490 4490
Size: 8215
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8215
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8215
SmaI CCC'GGG
Cuts at: 1872 4220 5542 1872
Size: 2348 1322 4545
Fragments arranged by size:
4545 2348 1322
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2236 2459 2831 3175 4825 6319
Size: 197 926 293 223 372 344 1650 1494
Cuts at: 6319 7220 7482 820
Size: 901 262 1553
Fragments arranged by size:
1650 1553 1494 926 901 372 344 293
262 223 197
SnaBI TAC'GTA
Cuts at: 493 5512 6006 493
Size: 5019 494 2702
Fragments arranged by size:
5019 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8215
SphI G_CATG'C
Cuts at: 1132 5573 1132
Size: 4441 3774
SspI AAT'ATT
Cuts at: 5 52 6139 5
Size: 47 6087 2081
Fragments arranged by size:
6087 2081 47
StuI AGG'CCT
Cuts at: 1517 3402 5166 1517
Size: 1885 1764 4566
Fragments arranged by size:
4566 1885 1764
StyI C'CwwG_G
Cuts at: 513 1065 2022 2388 2543 3075 4335 5654 513
Size: 552 957 366 155 532 1260 1319 3074
Fragments arranged by size:
3074 1319 1260 957 552 532 366 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2786 3871 4192 4224 5162 6242 6259
Size: 1255 1206 1085 321 32 938 1080 17
Cuts at: 6259 6412 6597 7042 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1206 1085 1080 938 445 321
185 153 32 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 3045 3562
Size: 274 80 33 51 1443 639 429 517
Cuts at: 3562 6461 8173 96
Size: 2899 1712 138
Fragments arranged by size:
2899 1712 1443 639 517 429 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3332 3364 3538 3570 4445 4477 8175 8207 3332
Size: 32 174 32 875 32 3698 32 3340
Fragments arranged by size:
3698 3340 875 174 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4234 1316
Size: 2918 5297
XbaI T'CTAG_A
Cuts at: 5551 5551
Size: 8215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2063 2663 4859 2063
Size: 600 2196 5419
Fragments arranged by size:
5419 2196 600
XhoI C'TCGA_G
Cuts at: 1943 2459 1943
Size: 516 7699
XmaI C'CCGG_G
Cuts at: 1870 4218 5540 1870
Size: 2348 1322 4545
Fragments arranged by size:
4545 2348 1322
XmnI GAAnn'nnTTC
Cuts at: 2043 3402 6344 2043
Size: 1359 2942 3914
Fragments arranged by size:
3914 2942 1359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6023 276
Size: 53 83 186 936 4489 2468
Fragments arranged by size:
4489 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI
RsrII SacII SanDI SapI SfoI SrfI SwaI