Restriction map for
pFN21ASDA0980
(Circular) (Six-base) MAPSORT of: pFN21ASDA0980.seq Check: 4054 from: 1 to: 8875
pFN21ASDA0980 8875 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3187 4218 3187
Size: 1031 7844
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5226 6685 278
Size: 53 83 186 936 3690 1459 2468
Fragments arranged by size:
3690 2468 1459 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 6218 1937
Size: 130 4151 4594
Fragments arranged by size:
4594 4151 130
Acc65I G'GTAC_C
Cuts at: 3348 6196 3348
Size: 2848 6027
AclI AA'CG_TT
Cuts at: 1563 7002 7375 1563
Size: 5439 373 3063
Fragments arranged by size:
5439 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2794 3049 3524 3599 3629 3695 4397 5313
Size: 1162 255 475 75 30 66 702 916
Cuts at: 5313 5810 5948 5990 6032 6038 6938 8322 8590
Size: 497 138 42 42 6 900 1384 268
Cuts at: 8590 1632
Size: 1917
Fragments arranged by size:
1917 1384 1162 916 900 702 497 475
268 255 138 75 66 42 42 30
6
AfeI AGC'GCT
Cuts at: 5172 5172
Size: 8875
AflII C'TTAA_G
Cuts at: 820 1017 2132 820
Size: 197 1115 7563
Fragments arranged by size:
7563 1115 197
AflIII A'CryG_T
Cuts at: 1203 2398 4550 5245 7774 8751 1203
Size: 1195 2152 695 2529 977 1327
Fragments arranged by size:
2529 2152 1327 1195 977 695
AgeI A'CCGG_T
Cuts at: 7706 7706
Size: 8875
AhdI GACnn_n'nnGTC
Cuts at: 7604 7604
Size: 8875
AleI CACnn'nnGTG
Cuts at: 1166 4891 1166
Size: 3725 5150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4180 4407 5157 1184
Size: 2996 227 750 4902
Fragments arranged by size:
4902 2996 750 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 5981 6013 3644
Size: 32 2305 32 6506
Fragments arranged by size:
6506 2305 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2076 2116 2155 2683 3004 3058 3178 3241 4177
Size: 40 39 528 321 54 120 63 936
Cuts at: 4177 4507 5572 5665 6618 8190 2076
Size: 330 1065 93 953 1572 2761
Fragments arranged by size:
2761 1572 1065 953 936 528 330 321
120 93 63 54 40 39
ApaI G_GGCC'C
Cuts at: 3109 4482 3109
Size: 1373 7502
ApaLI G'TGCA_C
Cuts at: 6932 8088 6932
Size: 1156 7719
ApoI r'AATT_y
Cuts at: 1493 2216 2792 6184 6410 6562 8671 1493
Size: 723 576 3392 226 152 2109 1697
Fragments arranged by size:
3392 2109 1697 723 576 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7429 160
Size: 872 6397 1606
Fragments arranged by size:
6397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
AvaI C'yCGr_G
Cuts at: 1870 1943 2720 2847 4133 4433 4836 5019 6200
Size: 73 777 127 1286 300 403 183 1181
Cuts at: 6200 1870
Size: 4545
Fragments arranged by size:
4545 1286 1181 777 403 300 183 127
73
BamHI G'GATC_C
Cuts at: 6205 6572 6205
Size: 367 8508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3348 3453 4156 4988 5030
Size: 325 217 627 1561 105 703 832 42
Cuts at: 5030 5782 5847 6080 6196 7651 618
Size: 752 65 233 116 1455 1842
Fragments arranged by size:
1842 1561 1455 832 752 703 627 325
233 217 116 105 65 42
BanII G_rGCy'C
Cuts at: 1391 2579 2712 2823 3109 3123 3642 3690 3791
Size: 1188 133 111 286 14 519 48 101
Cuts at: 3791 4068 4273 4482 4694 4837 5236 5731 6194
Size: 277 205 209 212 143 399 495 463
Cuts at: 6194 1391
Size: 4072
Fragments arranged by size:
4072 1188 519 495 463 399 286 277
212 209 205 143 133 111 101 48
14
BbeI G_GCGC'C
Cuts at: 3457 5034 5851 6084 3457
Size: 1577 817 233 6248
Fragments arranged by size:
6248 1577 817 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 2283 2745 2832 5226 928
Size: 971 339 45 462 87 2394 4577
Fragments arranged by size:
4577 2394 971 462 339 87 45
BbvCI CC'TCA_GC
Cuts at: 3465 4233 5741 3465
Size: 768 1508 6599
Fragments arranged by size:
6599 1508 768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7066 7100 1903
Size: 34 5129 34 3678
Fragments arranged by size:
5129 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6767 7977 6767
Size: 1210 7665
BclI T'GATC_A
Cuts at: 1548 2995 1548
Size: 1447 7428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3831 4545 4604
Size: 107 122 71 631 605 2159 714 59
Cuts at: 4604 7486 136
Size: 2882 1525
Fragments arranged by size:
2882 2159 1525 714 631 605 122 107
71 59
BglII A'GATC_T
Cuts at: 3045 8870 3045
Size: 5825 3050
BlpI GC'TnA_GC
Cuts at: 2449 3179 3362 3732 5819 6292 2449
Size: 730 183 370 2087 473 5032
Fragments arranged by size:
5032 2087 730 473 370 183
Bme1580I G_kGCm'C
Cuts at: 1790 3109 4375 4482 4550 4991 5787 6936 8092
Size: 1319 1266 107 68 441 796 1149 1156
Cuts at: 8092 1790
Size: 2573
Fragments arranged by size:
2573 1319 1266 1156 1149 796 441 107
68
BmgBI CAC'GTC
Cuts at: 1318 3448 1318
Size: 2130 6745
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5507 6606 7564
Size: 416 53 83 399 24 4090 1099 958
Cuts at: 7564 442
Size: 1753
Fragments arranged by size:
4090 1753 1099 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5020 5020
Size: 8875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2290 2480 2696 2926 2944 3113 3263
Size: 926 125 190 216 230 18 169 150
Cuts at: 3263 3287 3326 3359 3458 3617 3641 3843 3950
Size: 24 39 33 99 159 24 202 107
Cuts at: 3950 4058 4079 4325 4379 4385 4841 4851 4999
Size: 108 21 246 54 6 456 10 148
Cuts at: 4999 5057 5084 5195 5258 5294 5594 7535 1239
Size: 58 27 111 63 36 300 1941 2579
Fragments arranged by size:
2579 1941 926 456 300 246 230 216
202 190 169 159 150 148 125 111
108 107 99 63 58 54 39 36
33 27 24 24 21 18 10 6
Bpu10I CC'TnA_GC
Cuts at: 2057 2367 2669 3465 4140 4233 5741 2057
Size: 310 302 796 675 93 1508 5191
Fragments arranged by size:
5191 1508 796 675 310 302 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2807 3674 5720 7000 7865 8163 2807
Size: 867 2046 1280 865 298 3519
Fragments arranged by size:
3519 2046 1280 867 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2877 3926 4281 5153 7538 882
Size: 617 1378 1049 355 872 2385 2219
Fragments arranged by size:
2385 2219 1378 1049 872 617 355
BsaAI yAC'GTr
Cuts at: 493 1204 4553 6025 6172 6666 493
Size: 711 3349 1472 147 494 2702
Fragments arranged by size:
3349 2702 1472 711 494 147
BsaBI GATnn'nnATC
Cuts at: 8869 8869
Size: 8875
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 4869 5031 5223
Size: 53 83 186 936 1921 1415 162 192
Cuts at: 5223 5848 6081 6682 7064 7699 275
Size: 625 233 601 382 635 1451
Fragments arranged by size:
1921 1451 1415 936 635 625 601 382
233 192 186 162 83 53
BsaWI w'CCGG_w
Cuts at: 7308 7706 7980 8127 8538 7308
Size: 398 274 147 411 7645
Fragments arranged by size:
7645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3281 3311 3332 3362 3644
Size: 30 165 30 1878 30 21 30 282
Cuts at: 3644 3674 3795 3825 4430 4460 4466 4496 4542
Size: 30 121 30 605 30 6 30 46
Cuts at: 4542 4572 5030 5060 1178
Size: 30 458 30 4993
Fragments arranged by size:
4993 1878 605 458 282 165 121 46
30 30 30 30 30 30 30 30
30 30 21 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2906 3482 3668 4076 4322 4451 4454 4457
Size: 1720 576 186 408 246 129 3 3
Cuts at: 4457 4646 5549 5591 5936 6080 1186
Size: 189 903 42 345 144 3981
Fragments arranged by size:
3981 1720 903 576 408 345 246 189
186 144 129 42 3 3
BseYI C'CCAG_C
Cuts at: 2121 2644 3826 3924 4264 4852 4881 5472 5757
Size: 523 1182 98 340 588 29 591 285
Cuts at: 5757 8078 8574 2121
Size: 2321 496 2422
Fragments arranged by size:
2422 2321 1182 591 588 523 496 340
285 98 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3654 4150 4945 5741 1163
Size: 573 1918 496 795 796 4297
Fragments arranged by size:
4297 1918 796 795 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4917 6325 7086 8114 1540
Size: 275 186 2916 1408 761 1028 2301
Fragments arranged by size:
2916 2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2504 2942 3123 3642 3690 4068 4972 5436 5731
Size: 438 181 519 48 378 904 464 295
Cuts at: 5731 6052 6936 7021 8092 2504
Size: 321 884 85 1071 3287
Fragments arranged by size:
3287 1071 904 884 519 464 438 378
321 295 181 85 48
BsmI GAATG_Cn'
Cuts at: 5645 6386 6479 5645
Size: 741 93 8041
Fragments arranged by size:
8041 741 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2460 2763 4860 2460
Size: 303 2097 6475
Fragments arranged by size:
6475 2097 303
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2504 2579 2712 2823 2942 3109 3123
Size: 399 714 75 133 111 119 167 14
Cuts at: 3123 3642 3690 3791 4068 4273 4375 4482 4550
Size: 519 48 101 277 205 102 107 68
Cuts at: 4550 4694 4837 4972 4991 5236 5436 5731 5787
Size: 144 143 135 19 245 200 295 56
Cuts at: 5787 6052 6194 6936 7021 8092 1391
Size: 265 142 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 714 519 399 295 277
265 245 205 200 167 144 143 142
135 133 119 111 107 102 101 85
75 68 56 48 19 14
BspEI T'CCGG_A
Cuts at: 8538 8538
Size: 8875
BspHI T'CATG_A
Cuts at: 2815 3123 6764 8494 2815
Size: 308 3641 1730 3196
Fragments arranged by size:
3641 3196 1730 308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3187 3711 3883 4218 6012 6230 844
Size: 2343 524 172 335 1794 218 3489
Fragments arranged by size:
3489 2343 1794 524 335 218 172
BsrBI CCG'CTC
Cuts at: 1394 1823 2898 3658 4280 4588 5902 6762 1394
Size: 429 1075 760 622 308 1314 860 3507
Fragments arranged by size:
3507 1314 1075 860 760 622 429 308
BsrDI GCAATG_nn'
Cuts at: 65 1248 7370 7544 65
Size: 1183 6122 174 1396
Fragments arranged by size:
6122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2361 3768 4912 7519 7706 1700
Size: 110 551 1407 1144 2607 187 2869
Fragments arranged by size:
2869 2607 1407 1144 551 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8875
BssHII G'CGCG_C
Cuts at: 1925 4405 1925
Size: 2480 6395
BssSI C'ACGA_G
Cuts at: 2927 4070 6935 7947 2927
Size: 1143 2865 1012 3855
Fragments arranged by size:
3855 2865 1143 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 4507 5815 794
Size: 1361 2352 1308 3854
Fragments arranged by size:
3854 2352 1361 1308
BstBI TT'CG_AA
Cuts at: 2007 7679 2007
Size: 5672 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8875
BstXI CCAn_nnnn'nTGG
Cuts at: 4599 4893 4599
Size: 294 8581
BstYI r'GATC_y
Cuts at: 1972 2622 3045 6205 6572 6957 6974 8426 8512
Size: 650 423 3160 367 385 17 1452 86
Cuts at: 8512 8524 8870 1972
Size: 12 346 1977
Fragments arranged by size:
3160 1977 1452 650 423 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3917 4907 5735 3917
Size: 990 828 7057
Fragments arranged by size:
7057 990 828
BtgI C'CryG_G
Cuts at: 513 1065 2022 2085 4126 4459 4720 4886 5675
Size: 552 957 63 2041 333 261 166 789
Cuts at: 5675 513
Size: 3713
Fragments arranged by size:
3713 2041 957 789 552 333 261 166
63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5795 487
Size: 683 56 4569 3567
Fragments arranged by size:
4569 3567 683 56
BtsI GCAGTG_nn'
Cuts at: 4565 5596 6391 7185 7205 4565
Size: 1031 795 794 20 6235
Fragments arranged by size:
6235 1031 795 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8840
DraI TTT'AAA
Cuts at: 5394 6179 5394
Size: 785 8090
DraIII CAC_nnn'GTG
Cuts at: 3632 4181 5062 6043 3632
Size: 549 881 981 6464
Fragments arranged by size:
6464 981 881 549
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3140 7882 809
Size: 507 1824 4742 1802
Fragments arranged by size:
4742 1824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2350 3006 4537
Size: 54 1425 50 275 18 520 656 1531
Cuts at: 4537 4589 4914 5799 6322 7211 8840 8
Size: 52 325 885 523 889 1629 43
Fragments arranged by size:
1629 1531 1425 889 885 656 523 520
325 275 54 52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4914 6322 1537
Size: 275 3102 1408 4090
Fragments arranged by size:
4090 3102 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2484 2634 2813 2960 3251 4956 5145
Size: 675 456 150 179 147 291 1705 189
Cuts at: 5145 5892 5904 6805 1353
Size: 747 12 901 3423
Fragments arranged by size:
3423 1705 901 747 675 456 291 189
179 150 147 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3404 4253 5930 7462 7836 7982
Size: 1064 138 1815 849 1677 1532 374 146
Cuts at: 7982 387
Size: 1280
Fragments arranged by size:
1815 1677 1532 1280 1064 849 374 146
138
EcoICRI GAG'CTC
Cuts at: 3121 3640 3688 4066 5729 3121
Size: 519 48 378 1663 6267
Fragments arranged by size:
6267 1663 519 378 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2290 2480 2696 2794 2926 2944
Size: 393 533 125 190 216 98 132 18
Cuts at: 2944 3049 3113 3263 3287 3326 3359 3458 3524
Size: 105 64 150 24 39 33 99 66
Cuts at: 3524 3599 3617 3629 3641 3695 3843 3950 4058
Size: 75 18 12 12 54 148 107 108
Cuts at: 4058 4079 4325 4379 4385 4397 4841 4851 4999
Size: 21 246 54 6 12 444 10 148
Cuts at: 4999 5057 5084 5195 5258 5294 5313 5594 5810
Size: 58 27 111 63 36 19 281 216
Cuts at: 5810 5948 5990 6032 6038 6938 7535 8322 8590
Size: 138 42 42 6 900 597 787 268
Cuts at: 8590 1239
Size: 1524
Fragments arranged by size:
1524 900 787 597 533 444 393 281
268 246 216 216 190 150 148 148
138 132 125 111 108 107 105 99
98 75 66 64 63 58 54 54
42 42 39 36 33 27 24 21
19 18 18 12 12 12 10 6
6
EcoNI CCTnn'n_nnAGG
Cuts at: 3524 3647 4436 4732 5576 5648 3524
Size: 123 789 296 844 72 6751
Fragments arranged by size:
6751 844 789 296 123 72
EcoO109I rG'GnC_Cy
Cuts at: 2884 3001 3106 3238 3592 3700 3921 4096 4322
Size: 117 105 132 354 108 221 175 226
Cuts at: 4322 4479 4504 5082 2884
Size: 157 25 578 6677
Fragments arranged by size:
6677 578 354 226 221 175 157 132
117 108 105 25
EcoRI G'AATT_C
Cuts at: 2792 6184 6562 2792
Size: 3392 378 5105
Fragments arranged by size:
5105 3392 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2801 2801
Size: 8875
FseI GG_CCGG'CC
Cuts at: 4916 4916
Size: 8875
FspI TGC'GCA
Cuts at: 7381 7381
Size: 8875
HaeII r_GCGC'y
Cuts at: 1678 2449 3457 5034 5174 5851 6084 8022 1678
Size: 771 1008 1577 140 677 233 1938 2531
Fragments arranged by size:
2531 1938 1577 1008 771 677 233 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3281 3313 3795 3827 4466
Size: 32 135 32 1709 32 482 32 639
Cuts at: 4466 4498 4724 4756 5453 5485 7516 7548 7590
Size: 32 226 32 697 32 2031 32 42
Cuts at: 7590 7622 1373
Size: 32 2626
Fragments arranged by size:
2626 2031 1709 697 639 482 226 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 5562 6219 6465 669
Size: 988 281 268 3356 657 246 3079
Fragments arranged by size:
3356 3079 988 657 281 268 246
HindIII A'AGCT_T
Cuts at: 748 2135 3551 748
Size: 1387 1416 6072
Fragments arranged by size:
6072 1416 1387
HpaI GTT'AAC
Cuts at: 2206 5562 6465 2206
Size: 3356 903 4616
Fragments arranged by size:
4616 3356 903
KasI G'GCGC_C
Cuts at: 3453 5030 5847 6080 3453
Size: 1577 817 233 6248
Fragments arranged by size:
6248 1577 817 233
KpnI G_GTAC'C
Cuts at: 3352 6200 3352
Size: 2848 6027
MfeI C'AATT_G
Cuts at: 6474 6474
Size: 8875
MluI A'CGCG_T
Cuts at: 2398 8751 2398
Size: 6353 2522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2336 4329 4428 6024 7989 8173 1305
Size: 1031 1993 99 1596 1965 184 2007
Fragments arranged by size:
2007 1993 1965 1596 1031 184 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3008 4539 10
Size: 54 1425 343 1176 1531 4346
Fragments arranged by size:
4346 1531 1425 1176 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3128 4891 5555 6833 7192 7351 518
Size: 648 1962 1763 664 1278 359 159 2042
Fragments arranged by size:
2042 1962 1763 1278 664 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 2155 2300 2545 2661 3208 3928 4111
Size: 470 79 145 245 116 547 720 183
Cuts at: 4111 4128 4357 4461 4814 5671 5863 5884 6291
Size: 17 229 104 353 857 192 21 407
Cuts at: 6291 6968 8116 8361 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 857 720 677 547 470 407
353 245 245 229 192 183 145 116
104 79 21 17
NaeI GCC'GGC
Cuts at: 2363 4914 2363
Size: 2551 6324
NarI GG'CG_CC
Cuts at: 3454 5031 5848 6081 3454
Size: 1577 817 233 6248
Fragments arranged by size:
6248 1577 817 233
NcoI C'CATG_G
Cuts at: 513 1065 2022 5675 513
Size: 552 957 3653 3713
Fragments arranged by size:
3713 3653 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8875
NgoMIV G'CCGG_C
Cuts at: 2361 4912 2361
Size: 2551 6324
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8875
NotI GC'GGCC_GC
Cuts at: 6322 6322
Size: 8875
NspI r_CATG'y
Cuts at: 1132 1229 4750 5249 6233 7778 1132
Size: 97 3521 499 984 1545 2229
Fragments arranged by size:
3521 2229 1545 984 499 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8875
PciI A'CATG_T
Cuts at: 5245 7774 5245
Size: 2529 6346
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4616 1108
Size: 3508 5367
PmeI GTTT'AAAC
Cuts at: 6179 6179
Size: 8875
PmlI CAC'GTG
Cuts at: 4553 4553
Size: 8875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 6944 6976 8457 8489 3644
Size: 32 3268 32 1481 32 4030
Fragments arranged by size:
4030 3268 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 3001 3238 3592 3700 3921 4322 5082 2884
Size: 117 237 354 108 221 401 760 6677
Fragments arranged by size:
6677 760 401 354 237 221 117 108
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8875
PsiI TTA'TAA
Cuts at: 6445 6445
Size: 8875
PspOMI G'GGCC_C
Cuts at: 3105 4478 3105
Size: 1373 7502
PspXI vC'TCGA_Gb
Cuts at: 4433 4433
Size: 8875
PstI C_TGCA'G
Cuts at: 830 2172 2550 2594 3462 3588 3708 4104 4116
Size: 1342 378 44 868 126 120 396 12
Cuts at: 4116 4215 4386 5353 5670 6227 830
Size: 99 171 967 317 557 3478
Fragments arranged by size:
3478 1342 967 868 557 396 378 317
171 126 120 99 44 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8875
PvuII CAG'CTG
Cuts at: 2076 2155 2300 2545 3208 3928 4111 4357 5671
Size: 79 145 245 663 720 183 246 1314
Cuts at: 5671 5884 2076
Size: 213 5067
Fragments arranged by size:
5067 1314 720 663 246 245 213 183
145 79
SacI G_AGCT'C
Cuts at: 3123 3642 3690 4068 5731 3123
Size: 519 48 378 1663 6267
Fragments arranged by size:
6267 1663 519 378 48
SacII CC_GC'GG
Cuts at: 4129 4462 4129
Size: 333 8542
SalI G'TCGA_C
Cuts at: 1936 6217 1936
Size: 4281 4594
SanDI GG'GwC_CC
Cuts at: 4322 4322
Size: 8875
SapI GCTCTTCn'nnn_
Cuts at: 2634 4956 5892 2634
Size: 2322 936 5617
Fragments arranged by size:
5617 2322 936
SbfI CC_TGCA'GG
Cuts at: 3462 3708 4104 6227 3462
Size: 246 396 2123 6110
Fragments arranged by size:
6110 2123 396 246
ScaI AGT'ACT
Cuts at: 5359 7123 8835 5359
Size: 1764 1712 5399
Fragments arranged by size:
5399 1764 1712
SexAI A'CCwGG_T
Cuts at: 2880 5715 6039 2880
Size: 2835 324 5716
Fragments arranged by size:
5716 2835 324
SfcI C'TryA_G
Cuts at: 826 1045 2068 2168 2546 2590 3458 3521 3584
Size: 219 1023 100 378 44 868 63 63
Cuts at: 3584 3704 4100 4112 4211 4382 4946 5349 5666
Size: 120 396 12 99 171 564 403 317
Cuts at: 5666 6223 7358 8039 8230 826
Size: 557 1135 681 191 1471
Fragments arranged by size:
1471 1135 1023 868 681 564 557 403
396 378 317 219 191 171 120 100
99 63 63 44 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 4604 4604
Size: 8875
SfoI GGC'GCC
Cuts at: 3455 5032 5849 6082 3455
Size: 1577 817 233 6248
Fragments arranged by size:
6248 1577 817 233
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8875
SmaI CCC'GGG
Cuts at: 1872 4838 5021 6202 1872
Size: 2966 183 1181 4545
Fragments arranged by size:
4545 2966 1181 183
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 2720 2786 3689 4433 5699
Size: 197 926 189 588 66 903 744 1266
Cuts at: 5699 6979 7880 8142 820
Size: 1280 901 262 1553
Fragments arranged by size:
1553 1280 1266 926 903 901 744 588
262 197 189 66
SnaBI TAC'GTA
Cuts at: 493 6172 6666 493
Size: 5679 494 2702
Fragments arranged by size:
5679 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8875
SphI G_CATG'C
Cuts at: 1132 4750 6233 1132
Size: 3618 1483 3774
Fragments arranged by size:
3774 3618 1483
SrfI GCCC'GGGC
Cuts at: 5021 5021
Size: 8875
SspI AAT'ATT
Cuts at: 5 52 6799 5
Size: 47 6747 2081
Fragments arranged by size:
6747 2081 47
StuI AGG'CCT
Cuts at: 1517 3744 5003 5414 1517
Size: 2227 1259 411 4978
Fragments arranged by size:
4978 2227 1259 411
StyI C'CwwG_G
Cuts at: 513 1065 2022 3936 4296 5675 6314 513
Size: 552 957 1914 360 1379 639 3074
Fragments arranged by size:
3074 1914 1379 957 639 552 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4184 5006 5034 6902 6919 7072 7257
Size: 1255 2604 822 28 1868 17 153 185
Cuts at: 7257 7702 325
Size: 445 1498
Fragments arranged by size:
2604 1868 1498 1255 822 445 185 153
28 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5357 7121 8833
Size: 274 80 33 51 1443 3380 1764 1712
Cuts at: 8833 96
Size: 138
Fragments arranged by size:
3380 1764 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3731 3763 4542 4574 5030 5062 8835 8867 3731
Size: 32 779 32 456 32 3773 32 3739
Fragments arranged by size:
3773 3739 779 456 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5225 1316
Size: 3909 4966
XbaI T'CTAG_A
Cuts at: 2833 6211 2833
Size: 3378 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4178 5170 5848 4178
Size: 992 678 7205
Fragments arranged by size:
7205 992 678
XhoI C'TCGA_G
Cuts at: 1943 2720 4433 1943
Size: 777 1713 6385
Fragments arranged by size:
6385 1713 777
XmaI C'CCGG_G
Cuts at: 1870 4836 5019 6200 1870
Size: 2966 183 1181 4545
Fragments arranged by size:
4545 2966 1181 183
XmnI GAAnn'nnTTC
Cuts at: 6114 7004 6114
Size: 890 7985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5224 6683 276
Size: 53 83 186 936 3690 1459 2468
Fragments arranged by size:
3690 2468 1459 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PfoI PsrI RsrII SwaI