Restriction map for
pFN21ASDA0965
(Circular) (Six-base) MAPSORT of: pFN21ASDA0965.seq Check: 8776 from: 1 to: 6121
pFN21ASDA0965 6121 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 2404
Size: 6121
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3931 278
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 3464 1937
Size: 1243 284 4594
Fragments arranged by size:
4594 1243 284
Acc65I G'GTAC_C
Cuts at: 3064 3442 3064
Size: 378 5743
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4184 5568 5836 1632
Size: 2552 1384 268 1917
Fragments arranged by size:
2552 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 2293 2941 4850 2293
Size: 648 1909 3564
Fragments arranged by size:
3564 1909 648
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6121
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 2579
Size: 32 6089
AlwNI CAG_nnn'CTG
Cuts at: 2612 3864 5436 2612
Size: 1252 1572 3297
Fragments arranged by size:
3297 1572 1252
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 2110 2525 2756 3250 3430 3656 3808 5917
Size: 617 415 231 494 180 226 152 2109
Cuts at: 5917 1493
Size: 1697
Fragments arranged by size:
2109 1697 617 494 415 231 226 180
152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 3446 1870
Size: 73 130 1373 4545
Fragments arranged by size:
4545 1373 130 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 3064 3442 4897 618
Size: 325 217 627 675 602 378 1455 1842
Fragments arranged by size:
1842 1455 675 627 602 378 325 217
BanII G_rGCy'C
Cuts at: 1391 3440 1391
Size: 2049 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3538 3538
Size: 6121
Bme1580I G_kGCm'C
Cuts at: 1790 4182 5338 1790
Size: 2392 1156 2573
Fragments arranged by size:
2573 2392 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6121
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3193 3337 3852
Size: 416 53 83 399 24 1776 144 515
Cuts at: 3852 4810 442
Size: 958 1753
Fragments arranged by size:
1776 1753 958 515 416 399 144 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2556 2880 3042 4781 1239
Size: 1317 324 162 1739 2579
Fragments arranged by size:
2579 1739 1317 324 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2991 4246 5111 5409 2991
Size: 1255 865 298 3703
Fragments arranged by size:
3703 1255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4784 882
Size: 617 3285 2219
Fragments arranged by size:
3285 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3418 3912 493
Size: 711 2214 494 2702
Fragments arranged by size:
2702 2214 711 494
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3928 4310 4945 275
Size: 53 83 186 936 2395 382 635 1451
Fragments arranged by size:
2395 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3307 4554 4952 5226 5373 5784 3307
Size: 1247 398 274 147 411 3644
Fragments arranged by size:
3644 1247 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5896
Fragments arranged by size:
5896 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2331 1186
Size: 1145 4976
BseYI C'CCAG_C
Cuts at: 3404 5324 5820 3404
Size: 1920 496 3705
Fragments arranged by size:
3705 1920 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5548
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3571 4332 5360 1540
Size: 275 186 1570 761 1028 2301
Fragments arranged by size:
2301 1570 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3192 4182 4267 5338 3192
Size: 990 85 1071 3975
Fragments arranged by size:
3975 1071 990 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3192 3440 4182 4267 5338 1391
Size: 399 1402 248 742 85 1071 2174
Fragments arranged by size:
2174 1402 1071 742 399 248 85
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2404 3476 844
Size: 1560 1072 3489
Fragments arranged by size:
3489 1560 1072
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2034 2379 2552 2889 4616 4790 65
Size: 1183 786 345 173 337 1727 174 1396
Fragments arranged by size:
1727 1396 1183 786 345 337 174 173
BsrFI r'CCGG_y
Cuts at: 1700 1810 4765 4952 1700
Size: 110 2955 187 2869
Fragments arranged by size:
2955 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2462 2627 794
Size: 1668 165 4288
Fragments arranged by size:
4288 1668 165
BstBI TT'CG_AA
Cuts at: 2007 4925 2007
Size: 2918 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstYI r'GATC_y
Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116
Size: 1479 367 385 17 1452 86 12 346
Cuts at: 6116 1972
Size: 1977
Fragments arranged by size:
1977 1479 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2178 513
Size: 552 957 156 4456
Fragments arranged by size:
4456 957 552 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 3637 4431 4451 3637
Size: 794 20 5307
Fragments arranged by size:
5307 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DraIII CAC_nnn'GTG
Cuts at: 2789 2789
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 3568 4457
Size: 54 1425 50 275 18 345 1393 889
Cuts at: 4457 6086 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1393 889 345 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2199 2308 2577 4051 1353
Size: 783 63 109 269 1474 3423
Fragments arranged by size:
3423 1474 783 269 109 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2556 2880 3042 4184 4781 5568 5836
Size: 393 924 324 162 1142 597 787 268
Cuts at: 5836 1239
Size: 1524
Fragments arranged by size:
1524 1142 924 787 597 393 324 268
162
EcoNI CCTnn'n_nnAGG
Cuts at: 3352 3352
Size: 6121
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
EcoRV GAT'ATC
Cuts at: 2981 2981
Size: 6121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2337 2337
Size: 6121
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 5268 1678
Size: 3590 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 2927 2959 3302
Size: 32 135 32 707 32 616 32 343
Cuts at: 3302 3334 4762 4794 4836 4868 1373
Size: 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 707 616 343 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3181 3369 3465 3711 669
Size: 988 281 1243 188 96 246 3079
Fragments arranged by size:
3079 1243 988 281 246 188 96
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3068 3446 3068
Size: 378 5743
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 3954
Fragments arranged by size:
3954 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2367 3395 4079 4438 4597 518
Size: 648 861 340 1028 684 359 159 2042
Fragments arranged by size:
2042 1028 861 684 648 359 340 159
MspA1I CmG'CkG
Cuts at: 1606 3537 4214 5362 5607 1606
Size: 1931 677 1148 245 2120
Fragments arranged by size:
2120 1931 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2178 513
Size: 552 957 156 4456
Fragments arranged by size:
4456 957 552 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3479 5024 1132
Size: 97 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6121
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2641 3327 1108
Size: 1533 686 3902
Fragments arranged by size:
3902 1533 686
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2798 2830 4190 4222 5703 5735 2798
Size: 32 1360 32 1481 32 3184
Fragments arranged by size:
3184 1481 1360 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PstI C_TGCA'G
Cuts at: 830 3473 830
Size: 2643 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3179 3463 1936
Size: 1243 284 4594
Fragments arranged by size:
4594 1243 284
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2088 3219 3469 4604 5285 5476 826
Size: 219 1043 1131 250 1135 681 191 1471
Fragments arranged by size:
1471 1135 1131 1043 681 250 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 2075 3448 1872
Size: 203 1373 4545
Fragments arranged by size:
4545 1373 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3006 4225 5126 5388 820
Size: 197 926 1063 1219 901 262 1553
Fragments arranged by size:
1553 1219 1063 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3418 3912 493
Size: 2925 494 2702
Fragments arranged by size:
2925 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6121
StyI C'CwwG_G
Cuts at: 513 1065 2022 2178 2691 3084 3560 513
Size: 552 957 156 513 393 476 3074
Fragments arranged by size:
3074 957 552 513 476 393 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2458 4148 4165 4318 4503 4948 325
Size: 1255 878 1690 17 153 185 445 1498
Fragments arranged by size:
1690 1498 1255 878 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4367 6079 96
Size: 274 80 33 51 1443 2390 1712 138
Fragments arranged by size:
2390 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6081 6113 6081
Size: 32 6089
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6121
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2101 2278 2101
Size: 177 5944
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 2073 3446 1870
Size: 203 1373 4545
Fragments arranged by size:
4545 1373 203
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3929 276
Size: 53 83 186 936 2395 2468
Fragments arranged by size:
2468 2395 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI