Restriction map for
pFN21ASDA0964
(Circular) (Six-base) MAPSORT of: pFN21ASDA0964.seq Check: 5236 from: 1 to: 7696
pFN21ASDA0964 7696 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2858 3010 2858
Size: 152 7544
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5506 278
Size: 53 83 186 936 3970 2468
Fragments arranged by size:
3970 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5039 1937
Size: 3102 4594
Acc65I G'GTAC_C
Cuts at: 5017 5017
Size: 7696
AclI AA'CG_TT
Cuts at: 1563 5823 6196 1563
Size: 4260 373 3063
Fragments arranged by size:
4260 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2362 4539 5759 7143 7411 1632
Size: 730 2177 1220 1384 268 1917
Fragments arranged by size:
2177 1917 1384 1220 730 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7499
AflIII A'CryG_T
Cuts at: 1203 6595 7572 1203
Size: 5392 977 1327
Fragments arranged by size:
5392 1327 977
AgeI A'CCGG_T
Cuts at: 3709 6527 3709
Size: 2818 4878
AhdI GACnn_n'nnGTC
Cuts at: 6425 6425
Size: 7696
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3071 1184
Size: 1887 5809
AlwNI CAG_nnn'CTG
Cuts at: 2696 3238 3911 4567 4654 4916 5439 7011 2696
Size: 542 673 656 87 262 523 1572 3381
Fragments arranged by size:
3381 1572 673 656 542 523 262 87
ApaI G_GGCC'C
Cuts at: 3095 3095
Size: 7696
ApaLI G'TGCA_C
Cuts at: 5753 6909 5753
Size: 1156 6540
ApoI r'AATT_y
Cuts at: 1493 4580 5005 5231 5383 7492 1493
Size: 3087 425 226 152 2109 1697
Fragments arranged by size:
3087 2109 1697 425 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6250 160
Size: 872 5218 1606
Fragments arranged by size:
5218 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7696
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 3989 4364 4760 5021 1870
Size: 73 219 1827 375 396 261 4545
Fragments arranged by size:
4545 1827 396 375 261 219 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3850 3883 3850
Size: 33 7663
BamHI G'GATC_C
Cuts at: 5026 5393 5026
Size: 367 7329
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 2482 2669 3022 3204
Size: 325 217 627 479 216 187 353 182
Cuts at: 3204 4099 5017 6472 618
Size: 895 918 1455 1842
Fragments arranged by size:
1842 1455 918 895 627 479 353 325
217 216 187 182
BanII G_rGCy'C
Cuts at: 1391 2088 2282 2429 2601 2642 2900 3095 5015
Size: 697 194 147 172 41 258 195 1920
Cuts at: 5015 1391
Size: 4072
Fragments arranged by size:
4072 1920 697 258 195 194 172 147
41
BbeI G_GCGC'C
Cuts at: 2673 3208 2673
Size: 535 7161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2951 3704 928
Size: 971 1052 753 4920
Fragments arranged by size:
4920 1052 971 753
BbvCI CC'TCA_GC
Cuts at: 2918 2950 3647 2918
Size: 32 697 6967
Fragments arranged by size:
6967 697 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3059 3093 3567 3601 4690 4724 5887
Size: 34 1122 34 474 34 1089 34 1163
Cuts at: 5887 5921 1903
Size: 34 3678
Fragments arranged by size:
3678 1163 1122 1089 474 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4156 5588 6798 4156
Size: 1432 1210 5054
Fragments arranged by size:
5054 1432 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7696
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 2605 3575
Size: 107 122 71 631 605 478 455 970
Cuts at: 3575 3831 3914 4331 4422 4907 6307 136
Size: 256 83 417 91 485 1400 1525
Fragments arranged by size:
1525 1400 970 631 605 485 478 455
417 256 122 107 91 83 71
BglII A'GATC_T
Cuts at: 7691 7691
Size: 7696
BlpI GC'TnA_GC
Cuts at: 5113 5113
Size: 7696
Bme1580I G_kGCm'C
Cuts at: 1790 2501 2778 2873 3027 3095 4561 5757 6913
Size: 711 277 95 154 68 1466 1196 1156
Cuts at: 6913 1790
Size: 2573
Fragments arranged by size:
2573 1466 1196 1156 711 277 154 95
68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7696
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3400 3627 5427
Size: 416 53 83 399 24 1983 227 1800
Cuts at: 5427 6385 442
Size: 958 1753
Fragments arranged by size:
1983 1800 1753 958 416 399 227 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7696
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2207 4354 4390 2207
Size: 2147 36 5513
Fragments arranged by size:
5513 2147 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 3346 4360 6356 1239
Size: 1274 833 1014 1996 2579
Fragments arranged by size:
2579 1996 1274 1014 833
Bpu10I CC'TnA_GC
Cuts at: 2918 2950 3647 3836 2918
Size: 32 697 189 6778
Fragments arranged by size:
6778 697 189 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2960 4168 4712 5821 6686 6984 2960
Size: 1208 544 1109 865 298 3672
Fragments arranged by size:
3672 1208 1109 865 544 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 4490 4748 6359 882
Size: 617 2862 129 258 1611 2219
Fragments arranged by size:
2862 2219 1611 617 258 129
BsaAI yAC'GTr
Cuts at: 493 1204 4993 5487 493
Size: 711 3789 494 2702
Fragments arranged by size:
3789 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7690 7690
Size: 7696
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2670 3205 4334 4854
Size: 53 83 186 936 1137 535 1129 520
Cuts at: 4854 5503 5885 6520 275
Size: 649 382 635 1451
Fragments arranged by size:
1451 1137 1129 936 649 635 535 520
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3709 4795 6129 6527 6801 6948 7359 3709
Size: 1086 1334 398 274 147 411 4046
Fragments arranged by size:
4046 1334 1086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 3527 3557 3590
Size: 30 165 30 834 30 1260 30 33
Cuts at: 3590 3620 4220 4250 1178
Size: 30 600 30 4624
Fragments arranged by size:
4624 1260 834 600 165 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 3608 4096 4219 4363 1186
Size: 1102 1320 488 123 144 4519
Fragments arranged by size:
4519 1320 1102 488 144 123
BseYI C'CCAG_C
Cuts at: 2222 2288 2591 2689 2842 2855 3238 3318 3530
Size: 66 303 98 153 13 383 80 212
Cuts at: 3530 3878 3904 4384 4560 6899 7395 2222
Size: 348 26 480 176 2339 496 2523
Fragments arranged by size:
2523 2339 496 480 383 348 303 212
176 153 98 80 66 26 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 3087 3875 4089 5146 5907
Size: 275 186 106 980 788 214 1057 761
Cuts at: 5907 6935 1540
Size: 1028 2301
Fragments arranged by size:
2301 1057 1028 980 788 761 275 214
186 106
BsiHKAI G_wGCw'C
Cuts at: 2642 3050 5757 5842 6913 2642
Size: 408 2707 85 1071 3425
Fragments arranged by size:
3425 2707 1071 408 85
BsmI GAATG_Cn'
Cuts at: 5207 5300 5207
Size: 93 7603
BsmBI CGTCTCn'nnnn_
Cuts at: 3065 3503 4413 3065
Size: 438 910 6348
Fragments arranged by size:
6348 910 438
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2282 2429 2501 2601 2642 2778
Size: 399 298 194 147 72 100 41 136
Cuts at: 2778 2873 2900 3027 3050 3095 4561 5015 5757
Size: 95 27 127 23 45 1466 454 742
Cuts at: 5757 5842 6913 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1466 1071 742 454 399 298 194
147 136 127 100 95 85 72 45
41 27 23
BspEI T'CCGG_A
Cuts at: 7359 7359
Size: 7696
BspHI T'CATG_A
Cuts at: 5585 7315 5585
Size: 1730 5966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2858 3010 3024 3418 3570 4680 5051
Size: 1283 731 152 14 394 152 1110 371
Cuts at: 5051 844
Size: 3489
Fragments arranged by size:
3489 1283 1110 731 394 371 152 152
14
BsrBI CCG'CTC
Cuts at: 1394 1823 2581 2616 3543 4462 5583 1394
Size: 429 758 35 927 919 1121 3507
Fragments arranged by size:
3507 1121 927 919 758 429 35
BsrDI GCAATG_nn'
Cuts at: 65 1248 2527 6191 6365 65
Size: 1183 1279 3664 174 1396
Fragments arranged by size:
3664 1396 1279 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2316 2666 3105 3638 3709 4253 4393
Size: 110 506 350 439 533 71 544 140
Cuts at: 4393 4825 6340 6527 1700
Size: 432 1515 187 2869
Fragments arranged by size:
2869 1515 544 533 506 439 432 350
187 140 110 71
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7696
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7696
BssSI C'ACGA_G
Cuts at: 2081 2930 5756 6768 2081
Size: 849 2826 1012 3009
Fragments arranged by size:
3009 2826 1012 849
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7696
BstBI TT'CG_AA
Cuts at: 2007 6500 2007
Size: 4493 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7696
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2372 3383 3413 2335
Size: 37 1011 30 6618
Fragments arranged by size:
6618 1011 37 30
BstYI r'GATC_y
Cuts at: 1972 5026 5393 5778 5795 7247 7333 7345 7691
Size: 3054 367 385 17 1452 86 12 346
Cuts at: 7691 1972
Size: 1977
Fragments arranged by size:
3054 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3912 3912
Size: 7696
BtgI C'CryG_G
Cuts at: 513 1065 2130 2398 2416 3081 3138 3195 513
Size: 552 1065 268 18 665 57 57 5014
Fragments arranged by size:
5014 1065 665 552 268 57 57 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6957
Fragments arranged by size:
6957 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3490 3524 4561 4836 5212 6006 6026 2689
Size: 801 34 1037 275 376 794 20 4359
Fragments arranged by size:
4359 1037 801 794 376 275 34 20
ClaI AT'CG_AT
Cuts at: 1554 4019 4089 4248 1554
Size: 2465 70 159 5002
Fragments arranged by size:
5002 2465 159 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2398 2433 2497 2518 2532 2553 529
Size: 35 1834 35 64 21 14 21 5672
Fragments arranged by size:
5672 1834 64 35 35 21 21 14
DraI TTT'AAA
Cuts at: 5000 5000
Size: 7696
DraIII CAC_nnn'GTG
Cuts at: 3893 3893
Size: 7696
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3826 6703 809
Size: 507 2510 2877 1802
Fragments arranged by size:
2877 2510 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2431 3084 3415
Size: 54 1425 50 275 18 601 653 331
Cuts at: 3415 4899 5143 6032 7661 8
Size: 1484 244 889 1629 43
Fragments arranged by size:
1629 1484 1425 889 653 601 331 275
244 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3084 5143 1537
Size: 275 1272 2059 4090
Fragments arranged by size:
4090 2059 1272 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3318 4760 4770 5626 1353
Size: 1965 1442 10 856 3423
Fragments arranged by size:
3423 1965 1442 856 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2429 6283 6657 6803 387
Size: 1064 138 840 3854 374 146 1280
Fragments arranged by size:
3854 1280 1064 840 374 146 138
EcoICRI GAG'CTC
Cuts at: 2640 2640
Size: 7696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2362 2513 3346 4360 4539 5759 6356
Size: 393 730 151 833 1014 179 1220 597
Cuts at: 6356 7143 7411 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1220 1014 833 787 730 597 393
268 179 151
EcoNI CCTnn'n_nnAGG
Cuts at: 4064 4064
Size: 7696
EcoO109I rG'GnC_Cy
Cuts at: 3091 3092 3916 4267 4407 4597 4651 4669 3091
Size: 1 824 351 140 190 54 18 6118
Fragments arranged by size:
6118 824 351 190 140 54 18 1
EcoRI G'AATT_C
Cuts at: 5005 5383 5005
Size: 378 7318
EcoRV GAT'ATC
Cuts at: 4696 4696
Size: 7696
FspI TGC'GCA
Cuts at: 3116 6202 3116
Size: 3086 4610
HaeII r_GCGC'y
Cuts at: 1678 2673 3208 4873 6843 1678
Size: 995 535 1665 1970 2531
Fragments arranged by size:
2531 1970 1665 995 535
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3590 3622 4142 4174 4832
Size: 32 135 32 2018 32 520 32 658
Cuts at: 4832 4864 6337 6369 6411 6443 1373
Size: 32 1473 32 42 32 2626
Fragments arranged by size:
2626 2018 1473 658 520 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5040 5286 669
Size: 988 281 3102 246 3079
Fragments arranged by size:
3102 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7696
HpaI GTT'AAC
Cuts at: 5286 5286
Size: 7696
KasI G'GCGC_C
Cuts at: 2669 3204 2669
Size: 535 7161
KpnI G_GTAC'C
Cuts at: 5021 5021
Size: 7696
MfeI C'AATT_G
Cuts at: 5295 5295
Size: 7696
MluI A'CGCG_T
Cuts at: 7572 7572
Size: 7696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 2643 3828 6810 6994 1305
Size: 780 558 1185 2982 184 2007
Fragments arranged by size:
2982 2007 1185 780 558 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3417 10
Size: 54 1425 343 1585 4289
Fragments arranged by size:
4289 1585 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2370 5654 6013 6172 518
Size: 648 1204 3284 359 159 2042
Fragments arranged by size:
3284 2042 1204 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2356 2464 2504 3083 3120 3197 3366
Size: 606 144 108 40 579 37 77 169
Cuts at: 3366 3503 4681 4740 4747 5112 5789 6937 7182
Size: 137 1178 59 7 365 677 1148 245
Cuts at: 7182 1606
Size: 2120
Fragments arranged by size:
2120 1178 1148 677 606 579 365 245
169 144 137 108 77 59 40 37
7
NaeI GCC'GGC
Cuts at: 2668 3107 4395 4827 2668
Size: 439 1288 432 5537
Fragments arranged by size:
5537 1288 439 432
NarI GG'CG_CC
Cuts at: 2670 3205 2670
Size: 535 7161
NcoI C'CATG_G
Cuts at: 513 1065 3138 513
Size: 552 2073 5071
Fragments arranged by size:
5071 2073 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7696
NgoMIV G'CCGG_C
Cuts at: 2666 3105 4393 4825 2666
Size: 439 1288 432 5537
Fragments arranged by size:
5537 1288 439 432
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7696
NotI GC'GGCC_GC
Cuts at: 5143 5143
Size: 7696
NspI r_CATG'y
Cuts at: 1132 1229 2782 3115 3295 3941 5054 6599 1132
Size: 97 1553 333 180 646 1113 1545 2229
Fragments arranged by size:
2229 1553 1545 1113 646 333 180 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7696
PciI A'CATG_T
Cuts at: 6595 6595
Size: 7696
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2186 2696 4654 4740 1108
Size: 1078 510 1958 86 4064
Fragments arranged by size:
4064 1958 1078 510 86
PfoI T'CCnGG_A
Cuts at: 2334 4212 4968 2334
Size: 1878 756 5062
Fragments arranged by size:
5062 1878 756
PmeI GTTT'AAAC
Cuts at: 5000 5000
Size: 7696
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 5765 5797 7278 7310 4499
Size: 32 1234 32 1481 32 4885
Fragments arranged by size:
4885 1481 1234 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4651 4669 4651
Size: 18 7678
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7696
PsiI TTA'TAA
Cuts at: 5266 5266
Size: 7696
PspOMI G'GGCC_C
Cuts at: 3091 3091
Size: 7696
PstI C_TGCA'G
Cuts at: 830 3021 3485 3615 4449 5048 830
Size: 2191 464 130 834 599 3478
Fragments arranged by size:
3478 2191 834 599 464 130
PvuI CG_AT'CG
Cuts at: 2001 4089 2001
Size: 2088 5608
PvuII CAG'CTG
Cuts at: 2212 2356 3366 4681 4740 4747 2212
Size: 144 1010 1315 59 7 5161
Fragments arranged by size:
5161 1315 1010 144 59 7
SacI G_AGCT'C
Cuts at: 2642 2642
Size: 7696
SacII CC_GC'GG
Cuts at: 3084 3198 3084
Size: 114 7582
SalI G'TCGA_C
Cuts at: 1936 5038 1936
Size: 3102 4594
SbfI CC_TGCA'GG
Cuts at: 3021 5048 3021
Size: 2027 5669
ScaI AGT'ACT
Cuts at: 3766 5944 7656 3766
Size: 2178 1712 3806
Fragments arranged by size:
3806 2178 1712
SexAI A'CCwGG_T
Cuts at: 2445 2445
Size: 7696
SfcI C'TryA_G
Cuts at: 826 1045 3017 3481 3611 4445 4676 5044 6179
Size: 219 1972 464 130 834 231 368 1135
Cuts at: 6179 6860 7051 826
Size: 681 191 1471
Fragments arranged by size:
1972 1471 1135 834 681 464 368 231
219 191 130
SfoI GGC'GCC
Cuts at: 2671 3206 2671
Size: 535 7161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7696
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7696
SmaI CCC'GGG
Cuts at: 1872 2164 5023 1872
Size: 292 2859 4545
Fragments arranged by size:
4545 2859 292
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2975 4183 4364 4727 5800 6701
Size: 197 926 1032 1208 181 363 1073 901
Cuts at: 6701 6963 820
Size: 262 1553
Fragments arranged by size:
1553 1208 1073 1032 926 901 363 262
197 181
SnaBI TAC'GTA
Cuts at: 493 4993 5487 493
Size: 4500 494 2702
Fragments arranged by size:
4500 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7696
SphI G_CATG'C
Cuts at: 1132 3115 5054 1132
Size: 1983 1939 3774
Fragments arranged by size:
3774 1983 1939
SspI AAT'ATT
Cuts at: 5 52 5620 5
Size: 47 5568 2081
Fragments arranged by size:
5568 2081 47
StuI AGG'CCT
Cuts at: 1517 2031 2138 3392 4847 1517
Size: 514 107 1254 1455 4366
Fragments arranged by size:
4366 1455 1254 514 107
StyI C'CwwG_G
Cuts at: 513 1065 2409 2478 2586 2860 2958 3138 5135
Size: 552 1344 69 108 274 98 180 1997
Cuts at: 5135 513
Size: 3074
Fragments arranged by size:
3074 1997 1344 552 274 180 108 98
69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 3202 5723 5740 5893 6078 6523
Size: 1255 537 1085 2521 17 153 185 445
Cuts at: 6523 325
Size: 1498
Fragments arranged by size:
2521 1498 1255 1085 537 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3764 5942 7654
Size: 274 80 33 51 1443 1787 2178 1712
Cuts at: 7654 96
Size: 138
Fragments arranged by size:
2178 1787 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 4121 4153 4220 4252 4460 4492 7656
Size: 32 1852 32 67 32 208 32 3164
Cuts at: 7656 7688 2237
Size: 32 2245
Fragments arranged by size:
3164 2245 1852 208 67 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3602 1316
Size: 2286 5410
XbaI T'CTAG_A
Cuts at: 5032 5032
Size: 7696
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2396 2863 4356 2396
Size: 467 1493 5736
Fragments arranged by size:
5736 1493 467
XhoI C'TCGA_G
Cuts at: 1943 4364 1943
Size: 2421 5275
XmaI C'CCGG_G
Cuts at: 1870 2162 5021 1870
Size: 292 2859 4545
Fragments arranged by size:
4545 2859 292
XmnI GAAnn'nnTTC
Cuts at: 5825 5825
Size: 7696
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5504 276
Size: 53 83 186 936 3970 2468
Fragments arranged by size:
3970 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I FalI
FseI FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SanDI SapI SfiI SrfI SwaI