Restriction map for
pFN21ASDA0963
(Circular) (Six-base) MAPSORT of: pFN21ASDA0963.seq Check: 3673 from: 1 to: 8827
pFN21ASDA0963 8827 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2919 5083 5119 2919
Size: 2164 36 6627
Fragments arranged by size:
6627 2164 36
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5577 6637 278
Size: 53 83 186 936 4041 1060 2468
Fragments arranged by size:
4041 2468 1060 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3273 3528 6170 1937
Size: 1336 255 2642 4594
Fragments arranged by size:
4594 2642 1336 255
Acc65I G'GTAC_C
Cuts at: 6148 6148
Size: 8827
AclI AA'CG_TT
Cuts at: 1563 6954 7327 1563
Size: 5391 373 3063
Fragments arranged by size:
5391 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3843 4184 5186 5630 6890 8274 8542 1632
Size: 2211 341 1002 444 1260 1384 268 1917
Fragments arranged by size:
2211 1917 1384 1260 1002 444 341 268
AfeI AGC'GCT
Cuts at: 3647 4382 3647
Size: 735 8092
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8630
AflIII A'CryG_T
Cuts at: 1203 3567 7726 8703 1203
Size: 2364 4159 977 1327
Fragments arranged by size:
4159 2364 1327 977
AgeI A'CCGG_T
Cuts at: 4548 7658 4548
Size: 3110 5717
AhdI GACnn_n'nnGTC
Cuts at: 2362 7556 2362
Size: 5194 3633
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3026 3248 5609 1184
Size: 1842 222 2361 4402
Fragments arranged by size:
4402 2361 1842 222
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4598 4630 4598
Size: 32 8795
AlwNI CAG_nnn'CTG
Cuts at: 3139 4174 4345 4846 5445 5497 5683 6570 8142
Size: 1035 171 501 599 52 186 887 1572
Cuts at: 8142 3139
Size: 3824
Fragments arranged by size:
3824 1572 1035 887 599 501 186 171
52
ApaI G_GGCC'C
Cuts at: 2040 2761 4657 4738 5210 2040
Size: 721 1896 81 472 5657
Fragments arranged by size:
5657 1896 721 472 81
ApaLI G'TGCA_C
Cuts at: 3011 4484 4952 6884 8040 3011
Size: 1473 468 1932 1156 3798
Fragments arranged by size:
3798 1932 1473 1156 468
ApoI r'AATT_y
Cuts at: 1493 6136 6362 6514 8623 1493
Size: 4643 226 152 2109 1697
Fragments arranged by size:
4643 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7381 160
Size: 872 6349 1606
Fragments arranged by size:
6349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8827
AvaI C'yCGr_G
Cuts at: 1870 1943 3702 3915 4034 4136 4432 4731 5108
Size: 73 1759 213 119 102 296 299 377
Cuts at: 5108 5651 6062 6152 1870
Size: 543 411 90 4545
Fragments arranged by size:
4545 1759 543 411 377 299 296 213
119 102 90 73
BamHI G'GATC_C
Cuts at: 4100 6157 6524 4100
Size: 2057 367 6403
Fragments arranged by size:
6403 2057 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3290 3404 3795 4476 4727
Size: 325 217 627 1503 114 391 681 251
Cuts at: 4727 5112 5508 5719 5854 5897 6104 6148 7603
Size: 385 396 211 135 43 207 44 1455
Cuts at: 7603 618
Size: 1842
Fragments arranged by size:
1842 1503 1455 681 627 396 391 385
325 251 217 211 207 135 114 44
43
BanII G_rGCy'C
Cuts at: 1391 2040 2204 2761 4083 4233 4657 4738 5210
Size: 649 164 557 1322 150 424 81 472
Cuts at: 5210 5397 6146 1391
Size: 187 749 4072
Fragments arranged by size:
4072 1322 749 649 557 472 424 187
164 150 81
BbeI G_GCGC'C
Cuts at: 3408 4480 5723 5858 6108 3408
Size: 1072 1243 135 250 6127
Fragments arranged by size:
6127 1243 1072 250 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4573 5128 5618 928
Size: 971 2674 555 490 4137
Fragments arranged by size:
4137 2674 971 555 490
BbvCI CC'TCA_GC
Cuts at: 2586 4697 2586
Size: 2111 6716
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2423 2457 3104 3138 3433 3467 3708
Size: 34 486 34 647 34 295 34 241
Cuts at: 3708 3742 7018 7052 1903
Size: 34 3276 34 3678
Fragments arranged by size:
3678 3276 647 486 295 241 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4793 6719 7929 4793
Size: 1926 1210 5691
Fragments arranged by size:
5691 1926 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 4197 5567
Size: 107 122 71 631 605 583 1942 1370
Cuts at: 5567 7438 136
Size: 1871 1525
Fragments arranged by size:
1942 1871 1525 1370 631 605 583 122
107 71
BglII A'GATC_T
Cuts at: 5073 8822 5073
Size: 3749 5078
BlpI GC'TnA_GC
Cuts at: 2474 3020 3464 6244 2474
Size: 546 444 2780 5057
Fragments arranged by size:
5057 2780 546 444
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2616 2761 3015 3350 3798 4488 4657
Size: 250 576 145 254 335 448 690 169
Cuts at: 4657 4738 4794 4956 5115 5210 5900 6888 8044
Size: 81 56 162 159 95 690 988 1156
Cuts at: 8044 1790
Size: 2573
Fragments arranged by size:
2573 1156 988 690 690 576 448 335
254 250 169 162 159 145 95 81
56
BmgBI CAC'GTC
Cuts at: 1318 4897 5704 1318
Size: 3579 807 4441
Fragments arranged by size:
4441 3579 807
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4080 6558 7516
Size: 416 53 83 399 24 2663 2478 958
Cuts at: 7516 442
Size: 1753
Fragments arranged by size:
2663 2478 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8827
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2341
Size: 8827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2161 2251 2921 3620 4166 4411 4661 4827
Size: 922 90 670 699 546 245 250 166
Cuts at: 4827 4967 5354 5990 7487 1239
Size: 140 387 636 1497 2579
Fragments arranged by size:
2579 1497 922 699 670 636 546 387
250 245 166 140 90
Bpu10I CC'TnA_GC
Cuts at: 2298 2586 4697 4763 2298
Size: 288 2111 66 6362
Fragments arranged by size:
6362 2111 288 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2942 6952 7817 8115 2942
Size: 4010 865 298 3654
Fragments arranged by size:
4010 3654 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2615 2681 4116 4567 4662 7490 882
Size: 617 1116 66 1435 451 95 2828 2219
Fragments arranged by size:
2828 2219 1435 1116 617 451 95 66
BsaAI yAC'GTr
Cuts at: 493 1204 2638 4360 6124 6618 493
Size: 711 1434 1722 1764 494 2702
Fragments arranged by size:
2702 1764 1722 1434 711 494
BsaBI GATnn'nnATC
Cuts at: 8821 8821
Size: 8827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3060 3168 3405 3477
Size: 53 83 186 936 1527 108 237 72
Cuts at: 3477 3963 4477 5178 5574 5720 5855 5871 5883
Size: 486 514 701 396 146 135 16 12
Cuts at: 5883 5907 6105 6634 7016 7651 275
Size: 24 198 529 382 635 1451
Fragments arranged by size:
1527 1451 936 701 635 529 514 486
396 382 237 198 186 146 135 108
83 72 53 24 16 12
BsaWI w'CCGG_w
Cuts at: 3357 4548 7260 7658 7932 8079 8490 3357
Size: 1191 2712 398 274 147 411 3694
Fragments arranged by size:
3694 2712 1191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4010 4040 4490 4520 4547
Size: 30 165 30 2607 30 450 30 27
Cuts at: 4547 4577 4598 4628 1178
Size: 30 21 30 5377
Fragments arranged by size:
5377 2607 450 165 30 30 30 30
30 30 27 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 2335 2585 2588 2603 2606 2609 2618
Size: 1110 39 250 3 15 3 3 9
Cuts at: 2618 3383 3509 3722 3900 4018 5084 5096 5396
Size: 765 126 213 178 118 1066 12 300
Cuts at: 5396 6105 1186
Size: 709 3908
Fragments arranged by size:
3908 1110 1066 765 709 300 250 213
178 126 118 39 15 12 9 3
3 3
BseYI C'CCAG_C
Cuts at: 2608 2822 3511 3778 4195 4243 4254 5255 5893
Size: 214 689 267 417 48 11 1001 638
Cuts at: 5893 6019 8030 8526 2608
Size: 126 2011 496 2909
Fragments arranged by size:
2909 2011 1001 689 638 496 417 267
214 126 48 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 2576 3362 4724 5975 1163
Size: 573 375 465 786 1362 1251 4015
Fragments arranged by size:
4015 1362 1251 786 573 465 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2920 3585 3819 4096 4456 4519
Size: 275 186 919 665 234 277 360 63
Cuts at: 4519 4540 5320 5817 6277 7038 8066 1540
Size: 21 780 497 460 761 1028 2301
Fragments arranged by size:
2301 1028 919 780 761 665 497 460
360 277 275 234 186 63 21
BsiHKAI G_wGCw'C
Cuts at: 2204 3015 4233 4488 4956 6888 6973 8044 2204
Size: 811 1218 255 468 1932 85 1071 2987
Fragments arranged by size:
2987 1932 1218 1071 811 468 255 85
BsmI GAATG_Cn'
Cuts at: 3208 4969 6338 6431 3208
Size: 1761 1369 93 5604
Fragments arranged by size:
5604 1761 1369 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2950 3817 2950
Size: 867 7960
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2204 2616 2761 3015 3350 3798
Size: 399 250 164 412 145 254 335 448
Cuts at: 3798 4083 4233 4488 4657 4738 4794 4956 5115
Size: 285 150 255 169 81 56 162 159
Cuts at: 5115 5210 5397 5900 6146 6888 6973 8044 1391
Size: 95 187 503 246 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 503 448 412 399 335
285 255 254 250 246 187 169 164
162 159 150 145 95 85 81 56
BspEI T'CCGG_A
Cuts at: 3357 8490 3357
Size: 5133 3694
BspHI T'CATG_A
Cuts at: 2196 4386 6716 8446 2196
Size: 2190 2330 1730 2577
Fragments arranged by size:
2577 2330 2190 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 2919 2988 3249 5083 5119 5601 6182
Size: 1450 625 69 261 1834 36 482 581
Cuts at: 6182 844
Size: 3489
Fragments arranged by size:
3489 1834 1450 625 581 482 261 69
36
BsrBI CCG'CTC
Cuts at: 1394 1823 3890 4150 4159 4392 6067 6714 1394
Size: 429 2067 260 9 233 1675 647 3507
Fragments arranged by size:
3507 2067 1675 647 429 260 233 9
BsrDI GCAATG_nn'
Cuts at: 65 1248 7322 7496 65
Size: 1183 6074 174 1396
Fragments arranged by size:
6074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 2395 2861 3104 3206 3684 4191
Size: 110 267 318 466 243 102 478 507
Cuts at: 4191 4274 4548 4611 5319 5451 5460 5873 7471
Size: 83 274 63 708 132 9 413 1598
Cuts at: 7471 7658 1700
Size: 187 2869
Fragments arranged by size:
2869 1598 708 507 478 466 413 318
274 267 243 187 132 110 102 83
63 9
BsrGI T'GTAC_A
Cuts at: 96 2094 3447 5301 96
Size: 1998 1353 1854 3622
Fragments arranged by size:
3622 1998 1854 1353
BssHII G'CGCG_C
Cuts at: 1925 2833 3761 5529 6015 1925
Size: 908 928 1768 486 4737
Fragments arranged by size:
4737 1768 928 908 486
BssSI C'ACGA_G
Cuts at: 2807 4403 6887 7899 2807
Size: 1596 2484 1012 3735
Fragments arranged by size:
3735 2484 1596 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2554 4174 4846 5497 5602 5683 794
Size: 1760 1620 672 651 105 81 3938
Fragments arranged by size:
3938 1760 1620 672 651 105 81
BstBI TT'CG_AA
Cuts at: 2007 7631 2007
Size: 5624 3203
BstEII G'GTnAC_C
Cuts at: 1184 5371 1184
Size: 4187 4640
BstXI CCAn_nnnn'nTGG
Cuts at: 3107 3220 4639 5194 3107
Size: 113 1419 555 6740
Fragments arranged by size:
6740 1419 555 113
BstYI r'GATC_y
Cuts at: 1972 3135 3498 4100 4171 5073 5646 6157 6524
Size: 1163 363 602 71 902 573 511 367
Cuts at: 6524 6909 6926 8378 8464 8476 8822 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1163 902 602 573 511 385
367 363 346 86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 4367 4367
Size: 8827
BtgI C'CryG_G
Cuts at: 513 1065 2043 2253 2352 2997 3408 3423 3750
Size: 552 978 210 99 645 411 15 327
Cuts at: 3750 4505 4663 4680 5158 5242 5281 5376 513
Size: 755 158 17 478 84 39 95 3964
Fragments arranged by size:
3964 978 755 645 552 478 411 327
210 158 99 95 84 39 17 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2868 2989 3436 3652 5539 487
Size: 683 56 1642 121 447 216 1887 3775
Fragments arranged by size:
3775 1887 1642 683 447 216 121 56
BtsI GCAGTG_nn'
Cuts at: 2037 2128 5428 5455 6343 7137 7157 2037
Size: 91 3300 27 888 794 20 3707
Fragments arranged by size:
3707 3300 888 794 91 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8792
DraI TTT'AAA
Cuts at: 6131 6131
Size: 8827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3434 4058 4460 7834 809
Size: 507 2118 624 402 3374 1802
Fragments arranged by size:
3374 2118 1802 624 507 402
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2886 2917
Size: 54 1425 50 275 18 426 630 31
Cuts at: 2917 3109 3420 3582 3816 4407 4537 4599 4629
Size: 192 311 162 234 591 130 62 30
Cuts at: 4629 4867 5284 5317 5568 5814 6274 7163 8792
Size: 238 417 33 251 246 460 889 1629
Cuts at: 8792 8
Size: 43
Fragments arranged by size:
1629 1425 889 630 591 460 426 417
311 275 251 246 238 234 192 162
130 62 54 50 43 33 31 30
18
EagI C'GGCC_G
Cuts at: 1537 1812 2917 3582 3816 4537 5317 5814 6274
Size: 275 1105 665 234 721 780 497 460
Cuts at: 6274 1537
Size: 4090
Fragments arranged by size:
4090 1105 780 721 665 497 460 275
234
EarI CTCTTCn'nnn_
Cuts at: 1353 3071 3386 6757 1353
Size: 1718 315 3371 3423
Fragments arranged by size:
3423 3371 1718 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2194 2615 3070 4280 5285 5342
Size: 1064 138 605 421 455 1210 1005 57
Cuts at: 5342 7414 7788 7934 387
Size: 2072 374 146 1280
Fragments arranged by size:
2072 1280 1210 1064 1005 605 455 421
374 146 138 57
EcoICRI GAG'CTC
Cuts at: 2202 4231 2202
Size: 2029 6798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 2251 2921 3620 3843 4166 4184
Size: 393 529 90 670 699 223 323 18
Cuts at: 4184 4411 4661 4827 4967 5186 5354 5630 5990
Size: 227 250 166 140 219 168 276 360
Cuts at: 5990 6890 7487 8274 8542 1239
Size: 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 699 670 597 529 393
360 323 276 268 250 227 223 219
168 166 140 90 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2411 2411
Size: 8827
EcoO109I rG'GnC_Cy
Cuts at: 2036 2757 2758 2812 3559 4141 4653 4654 4735
Size: 721 1 54 747 582 512 1 81
Cuts at: 4735 4813 4843 5207 5338 2036
Size: 78 30 364 131 5525
Fragments arranged by size:
5525 747 721 582 512 364 131 81
78 54 30 1 1
EcoRI G'AATT_C
Cuts at: 6136 6514 6136
Size: 378 8449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4399 4726 4399
Size: 327 8500
FspI TGC'GCA
Cuts at: 7333 7333
Size: 8827
HaeII r_GCGC'y
Cuts at: 1678 2121 3285 3408 3649 4384 4480 4515 4612
Size: 443 1164 123 241 735 96 35 97
Cuts at: 4612 4639 5201 5445 5723 5768 5858 6108 7974
Size: 27 562 244 278 45 90 250 1866
Cuts at: 7974 1678
Size: 2531
Fragments arranged by size:
2531 1866 1164 735 562 443 278 250
244 241 123 97 96 90 45 35
27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2348 2380 4010 4042 4085
Size: 32 135 32 776 32 1630 32 43
Cuts at: 4085 4117 7468 7500 7542 7574 1373
Size: 32 3351 32 42 32 2626
Fragments arranged by size:
3351 2626 1630 776 135 43 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4216 6171 6417 669
Size: 988 281 2278 1955 246 3079
Fragments arranged by size:
3079 2278 1955 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8827
HpaI GTT'AAC
Cuts at: 6417 6417
Size: 8827
KasI G'GCGC_C
Cuts at: 3404 4476 5719 5854 6104 3404
Size: 1072 1243 135 250 6127
Fragments arranged by size:
6127 1243 1072 250 135
KpnI G_GTAC'C
Cuts at: 6152 6152
Size: 8827
MfeI C'AATT_G
Cuts at: 6426 6426
Size: 8827
MluI A'CGCG_T
Cuts at: 8703 8703
Size: 8827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 2352 2973 3978 4578 7941 8125 1305
Size: 1041 6 621 1005 600 3363 184 2007
Fragments arranged by size:
3363 2007 1041 1005 621 600 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 3111 3422 4409 4631
Size: 54 1425 343 426 853 311 987 222
Cuts at: 4631 10
Size: 4206
Fragments arranged by size:
4206 1425 987 853 426 343 311 222
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3008 3218 3428 5009 6785 7144 7303
Size: 648 1842 210 210 1581 1776 359 159
Cuts at: 7303 518
Size: 2042
Fragments arranged by size:
2042 1842 1776 1581 648 359 210 210
159
MspA1I CmG'CkG
Cuts at: 1606 2754 2826 3441 3505 3821 3959 4243 4258
Size: 1148 72 615 64 316 138 284 15
Cuts at: 4258 4474 5160 5244 5259 5283 5332 5378 5761
Size: 216 686 84 15 24 49 46 383
Cuts at: 5761 5860 5893 6243 6920 8068 8313 1606
Size: 99 33 350 677 1148 245 2120
Fragments arranged by size:
2120 1148 1148 686 677 615 383 350
316 284 245 216 138 99 84 72
64 49 46 33 24 15 15
NaeI GCC'GGC
Cuts at: 2079 2397 2863 3208 3686 4193 5321 5462 5875
Size: 318 466 345 478 507 1128 141 413
Cuts at: 5875 2079
Size: 5031
Fragments arranged by size:
5031 1128 507 478 466 413 345 318
141
NarI GG'CG_CC
Cuts at: 3405 4477 5720 5855 6105 3405
Size: 1072 1243 135 250 6127
Fragments arranged by size:
6127 1243 1072 250 135
NcoI C'CATG_G
Cuts at: 513 1065 2043 3408 3423 513
Size: 552 978 1365 15 5917
Fragments arranged by size:
5917 1365 978 552 15
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8827
NgoMIV G'CCGG_C
Cuts at: 2077 2395 2861 3206 3684 4191 5319 5460 5873
Size: 318 466 345 478 507 1128 141 413
Cuts at: 5873 2077
Size: 5031
Fragments arranged by size:
5031 1128 507 478 466 413 345 318
141
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8827
NotI GC'GGCC_GC
Cuts at: 6274 6274
Size: 8827
NspI r_CATG'y
Cuts at: 1132 1229 3448 5521 5980 6185 7730 1132
Size: 97 2219 2073 459 205 1545 2229
Fragments arranged by size:
2229 2219 2073 1545 459 205 97
PasI CC'CwG_GG
Cuts at: 1348 4795 5397 5728 1348
Size: 3447 602 331 4447
Fragments arranged by size:
4447 3447 602 331
PciI A'CATG_T
Cuts at: 7726 7726
Size: 8827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2427 4261 1108
Size: 1319 1834 5674
Fragments arranged by size:
5674 1834 1319
PfoI T'CCnGG_A
Cuts at: 2898 3138 4143 4827 4877 5046 2898
Size: 240 1005 684 50 169 6679
Fragments arranged by size:
6679 1005 684 240 169 50
PmeI GTTT'AAAC
Cuts at: 6131 6131
Size: 8827
PmlI CAC'GTG
Cuts at: 4360 4360
Size: 8827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2167 4598 4630 6896 6928 8409 8441 2135
Size: 32 2431 32 2266 32 1481 32 2521
Fragments arranged by size:
2521 2431 2266 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2812 4141 4813 2812
Size: 1329 672 6826
Fragments arranged by size:
6826 1329 672
PshAI GACnn'nnGTC
Cuts at: 1613 3186 1613
Size: 1573 7254
PsiI TTA'TAA
Cuts at: 6397 6397
Size: 8827
PspOMI G'GGCC_C
Cuts at: 2036 2757 4653 4734 5206 2036
Size: 721 1896 81 472 5657
Fragments arranged by size:
5657 1896 721 472 81
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 8795
PstI C_TGCA'G
Cuts at: 830 2115 2775 3246 3747 4119 5435 5493 5598
Size: 1285 660 471 501 372 1316 58 105
Cuts at: 5598 5679 6179 830
Size: 81 500 3478
Fragments arranged by size:
3478 1316 1285 660 501 500 471 372
105 81 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8827
PvuII CAG'CTG
Cuts at: 4243 5259 5332 4243
Size: 1016 73 7738
Fragments arranged by size:
7738 1016 73
SacI G_AGCT'C
Cuts at: 2204 4233 2204
Size: 2029 6798
SacII CC_GC'GG
Cuts at: 5161 5245 5284 5379 5161
Size: 84 39 95 8609
Fragments arranged by size:
8609 95 84 39
SalI G'TCGA_C
Cuts at: 1936 6169 1936
Size: 4233 4594
SanDI GG'GwC_CC
Cuts at: 4813 4813
Size: 8827
SbfI CC_TGCA'GG
Cuts at: 5493 6179 5493
Size: 686 8141
ScaI AGT'ACT
Cuts at: 4979 7075 8787 4979
Size: 2096 1712 5019
Fragments arranged by size:
5019 2096 1712
SexAI A'CCwGG_T
Cuts at: 2412 4557 2412
Size: 2145 6682
SfcI C'TryA_G
Cuts at: 826 1045 2111 2771 3242 3743 4115 5431 5489
Size: 219 1066 660 471 501 372 1316 58
Cuts at: 5489 5594 5675 5803 6175 7310 7991 8182 826
Size: 105 81 128 372 1135 681 191 1471
Fragments arranged by size:
1471 1316 1135 1066 681 660 501 471
372 372 219 191 128 105 81 58
SfoI GGC'GCC
Cuts at: 3406 4478 5721 5856 6106 3406
Size: 1072 1243 135 250 6127
Fragments arranged by size:
6127 1243 1072 250 135
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8827
SgrAI Cr'CCGG_yG
Cuts at: 1700 2077 1700
Size: 377 8450
SmaI CCC'GGG
Cuts at: 1872 4138 4733 5110 6154 1872
Size: 2266 595 377 1044 4545
Fragments arranged by size:
4545 2266 1044 595 377
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2957 6931 7832 8094 820
Size: 197 926 1014 3974 901 262 1553
Fragments arranged by size:
3974 1553 1014 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6124 6618 493
Size: 5631 494 2702
Fragments arranged by size:
5631 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8827
SphI G_CATG'C
Cuts at: 1132 6185 1132
Size: 5053 3774
SspI AAT'ATT
Cuts at: 5 52 6751 5
Size: 47 6699 2081
Fragments arranged by size:
6699 2081 47
StuI AGG'CCT
Cuts at: 1517 3158 4424 5822 1517
Size: 1641 1266 1398 4522
Fragments arranged by size:
4522 1641 1398 1266
StyI C'CwwG_G
Cuts at: 513 1065 2043 3408 3423 3675 4494 5388 5616
Size: 552 978 1365 15 252 819 894 228
Cuts at: 5616 6266 513
Size: 650 3074
Fragments arranged by size:
3074 1365 978 894 819 650 552 252
228 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2468 2685 4559 6854 6871 7024 7209
Size: 1255 888 217 1874 2295 17 153 185
Cuts at: 7209 7654 325
Size: 445 1498
Fragments arranged by size:
2295 1874 1498 1255 888 445 217 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3447 4704
Size: 274 80 33 51 1443 117 1353 1257
Cuts at: 4704 4977 5301 7073 8785 96
Size: 273 324 1772 1712 138
Fragments arranged by size:
1772 1712 1443 1353 1257 324 274 273
138 117 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3845 3877 4490 4522 8787 8819 3845
Size: 32 613 32 4265 32 3853
Fragments arranged by size:
4265 3853 613 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4058 1316
Size: 2742 6085
XbaI T'CTAG_A
Cuts at: 6163 6163
Size: 8827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 3415 4802 5263 2029
Size: 1386 1387 461 5593
Fragments arranged by size:
5593 1387 1386 461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8827
XmaI C'CCGG_G
Cuts at: 1870 4136 4731 5108 6152 1870
Size: 2266 595 377 1044 4545
Fragments arranged by size:
4545 2266 1044 595 377
XmnI GAAnn'nnTTC
Cuts at: 2147 3569 6956 2147
Size: 1422 3387 4018
Fragments arranged by size:
4018 3387 1422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5575 6635 276
Size: 53 83 186 936 4041 1060 2468
Fragments arranged by size:
4041 2468 1060 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV
FseI FspAI NruI NsiI PacI PspXI RsrII SapI
SfiI SrfI SwaI