Restriction map for
pFN21ASDA0959
(Circular) (Six-base) MAPSORT of: pFN21ASDA0959.seq Check: 4811 from: 1 to: 7033
pFN21ASDA0959 7033 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3977 3977
Size: 7033
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3917 4843 278
Size: 53 83 186 936 2381 926 2468
Fragments arranged by size:
2468 2381 936 926 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2980 4376 1937
Size: 1043 1396 4594
Fragments arranged by size:
4594 1396 1043
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 5160 5533 1563
Size: 3597 373 3063
Fragments arranged by size:
3597 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2641 3008 3308 4094 5096 6480 6748 1632
Size: 1009 367 300 786 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1009 1002 786 367 300 268
AfeI AGC'GCT
Cuts at: 3899 3899
Size: 7033
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6836
AflIII A'CryG_T
Cuts at: 1203 4008 5932 6909 1203
Size: 2805 1924 977 1327
Fragments arranged by size:
2805 1924 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 2192 5762 2192
Size: 3570 3463
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7033
AlwNI CAG_nnn'CTG
Cuts at: 2533 3478 3527 3719 4776 6348 2533
Size: 945 49 192 1057 1572 3218
Fragments arranged by size:
3218 1572 1057 945 192 49
ApaLI G'TGCA_C
Cuts at: 5090 6246 5090
Size: 1156 5877
ApoI r'AATT_y
Cuts at: 1493 2702 2992 3176 3374 3820 4342 4568 4720
Size: 1209 290 184 198 446 522 226 152
Cuts at: 4720 6829 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1209 522 446 290 226 198
184 152
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 4358 1870
Size: 73 2415 4545
Fragments arranged by size:
4545 2415 73
BamHI G'GATC_C
Cuts at: 4363 4730 4363
Size: 367 6666
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2237 3284 4354 5809 618
Size: 325 217 627 450 1047 1070 1455 1842
Fragments arranged by size:
1842 1455 1070 1047 627 450 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2508 2567 3662 3731 4352 1391
Size: 663 454 59 1095 69 621 4072
Fragments arranged by size:
4072 1095 663 621 454 69 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2499 3828 3969 3999 928
Size: 971 600 1329 141 30 3962
Fragments arranged by size:
3962 1329 971 600 141 30
BbvCI CC'TCA_GC
Cuts at: 3720 3720
Size: 7033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 5224 5258 1903
Size: 34 1979 34 1274 34 3678
Fragments arranged by size:
3678 1979 1274 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4925 6135 4925
Size: 1210 5823
BclI T'GATC_A
Cuts at: 1548 2439 3606 1548
Size: 891 1167 4975
Fragments arranged by size:
4975 1167 891
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5644 136
Size: 107 122 71 631 605 3972 1525
Fragments arranged by size:
3972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2722 7028 2722
Size: 4306 2727
BlpI GC'TnA_GC
Cuts at: 4450 4450
Size: 7033
Bme1580I G_kGCm'C
Cuts at: 1790 3276 3287 5094 6250 1790
Size: 1486 11 1807 1156 2573
Fragments arranged by size:
2573 1807 1486 1156 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2074 4764 5722
Size: 416 53 83 399 24 657 2690 958
Cuts at: 5722 442
Size: 1753
Fragments arranged by size:
2690 1753 958 657 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3745 3802 3745
Size: 57 6976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 2702 3224 4318 5693 1239
Size: 927 536 522 1094 1375 2579
Fragments arranged by size:
2579 1375 1094 927 536 522
Bpu10I CC'TnA_GC
Cuts at: 3720 3720
Size: 7033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 3025 5158 6023 6321 2618
Size: 407 2133 865 298 3330
Fragments arranged by size:
3330 2133 865 407 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2811 5696 882
Size: 617 1312 2885 2219
Fragments arranged by size:
2885 2219 1312 617
BsaAI yAC'GTr
Cuts at: 493 1204 2709 4330 4824 493
Size: 711 1505 1621 494 2702
Fragments arranged by size:
2702 1621 1505 711 494
BsaBI GATnn'nnATC
Cuts at: 2963 7027 2963
Size: 4064 2969
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3531 3914 4840 5222
Size: 53 83 186 936 1998 383 926 382
Cuts at: 5222 5857 275
Size: 635 1451
Fragments arranged by size:
1998 1451 936 926 635 383 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5466 5864 6138 6285 6696 5466
Size: 398 274 147 411 5803
Fragments arranged by size:
5803 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2636 2666 3649 3679 3839
Size: 30 165 30 1233 30 983 30 160
Cuts at: 3839 3869 1178
Size: 30 4342
Fragments arranged by size:
4342 1233 983 165 160 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3500 3503 3737 3775 3847 4118 1186
Size: 2314 3 234 38 72 271 4101
Fragments arranged by size:
4101 2314 271 234 72 38 3
BseYI C'CCAG_C
Cuts at: 6236 6732 6236
Size: 496 6537
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2508 4139 1163
Size: 573 772 1631 4057
Fragments arranged by size:
4057 1631 772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4483 5244 6272 1540
Size: 275 186 2482 761 1028 2301
Fragments arranged by size:
2482 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 2587 3662 5094 5179 6250 2054
Size: 533 1075 1432 85 1071 2837
Fragments arranged by size:
2837 1432 1075 1071 533 85
BsmI GAATG_Cn'
Cuts at: 2452 3090 4544 4637 2452
Size: 638 1454 93 4848
Fragments arranged by size:
4848 1454 638 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2508 2567 2587 3276 3287 3662
Size: 399 264 454 59 20 689 11 375
Cuts at: 3662 3731 4352 5094 5179 6250 1391
Size: 69 621 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 689 621 454 399 375
264 85 69 59 20 11
BspEI T'CCGG_A
Cuts at: 6696 6696
Size: 7033
BspHI T'CATG_A
Cuts at: 2971 4922 6652 2971
Size: 1951 1730 3352
Fragments arranged by size:
3352 1951 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 3977 4111 4388 844
Size: 1427 1706 134 277 3489
Fragments arranged by size:
3489 1706 1427 277 134
BsrBI CCG'CTC
Cuts at: 1394 1823 4920 1394
Size: 429 3097 3507
Fragments arranged by size:
3507 3097 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5528 5702 65
Size: 1183 4280 174 1396
Fragments arranged by size:
4280 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5677 5864 1700
Size: 110 3867 187 2869
Fragments arranged by size:
3867 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4011 96
Size: 3915 3118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 2726 5093 6105 2726
Size: 2367 1012 3654
Fragments arranged by size:
3654 2367 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7033
BstBI TT'CG_AA
Cuts at: 2007 5837 2007
Size: 3830 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstYI r'GATC_y
Cuts at: 1972 2722 4363 4730 5115 5132 6584 6670 6682
Size: 750 1641 367 385 17 1452 86 12
Cuts at: 6682 7028 1972
Size: 346 1977
Fragments arranged by size:
1977 1641 1452 750 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3826 3826
Size: 7033
BtgI C'CryG_G
Cuts at: 513 1065 3741 3810 4227 513
Size: 552 2676 69 417 3319
Fragments arranged by size:
3319 2676 552 417 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2950 487
Size: 683 56 1724 4570
Fragments arranged by size:
4570 1724 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 3023 3202 3315 3645 4549 5343 5363 2785
Size: 238 179 113 330 904 794 20 4455
Fragments arranged by size:
4455 904 794 330 238 179 113 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 4337 4337
Size: 7033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4480 5369 6998
Size: 54 1425 50 275 18 2650 889 1629
Cuts at: 6998 8
Size: 43
Fragments arranged by size:
2650 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4480 1537
Size: 275 2668 4090
Fragments arranged by size:
4090 2668 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2575 2661 2667 2768 3554 3566 4963
Size: 714 508 86 6 101 786 12 1397
Cuts at: 4963 1353
Size: 3423
Fragments arranged by size:
3423 1397 786 714 508 101 86 12
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2052 3660 2052
Size: 1608 5425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2641 2702 3008 3224 3308 4094
Size: 393 534 475 61 306 216 84 786
Cuts at: 4094 4318 5096 5693 6480 6748 1239
Size: 224 778 597 787 268 1524
Fragments arranged by size:
1524 787 786 778 597 534 475 393
306 268 224 216 84 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2666 3004 3482 2666
Size: 338 478 6217
Fragments arranged by size:
6217 478 338
EcoO109I rG'GnC_Cy
Cuts at: 2469 2469
Size: 7033
EcoRI G'AATT_C
Cuts at: 2702 4342 4720 2702
Size: 1640 378 5015
Fragments arranged by size:
5015 1640 378
EcoRV GAT'ATC
Cuts at: 3112 3112
Size: 7033
FspI TGC'GCA
Cuts at: 4214 5539 4214
Size: 1325 5708
HaeII r_GCGC'y
Cuts at: 1678 3901 6180 1678
Size: 2223 2279 2531
Fragments arranged by size:
2531 2279 2223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2178 2210 2928 2960 3101
Size: 32 135 32 606 32 718 32 141
Cuts at: 3101 3133 3598 3630 5674 5706 5748 5780 1373
Size: 32 465 32 2044 32 42 32 2626
Fragments arranged by size:
2626 2044 718 606 465 141 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4029 4377 4623 669
Size: 988 281 2091 348 246 3079
Fragments arranged by size:
3079 2091 988 348 281 246
HindIII A'AGCT_T
Cuts at: 748 2249 748
Size: 1501 5532
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6147 6331 1305
Size: 4842 184 2007
Fragments arranged by size:
4842 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5211
Fragments arranged by size:
5211 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3254 3986 4991 5350 5509 518
Size: 648 1386 702 732 1005 359 159 2042
Fragments arranged by size:
2042 1386 1005 732 702 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2176 2740 3524 3654 4052 4449 5126 6274
Size: 570 564 784 130 398 397 677 1148
Cuts at: 6274 6519 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 784 677 570 564 398 397
245 130
NcoI C'CATG_G
Cuts at: 513 1065 3741 3810 4227 513
Size: 552 2676 69 417 3319
Fragments arranged by size:
3319 2676 552 417 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 4012 4391 5936 1132
Size: 97 2783 379 1545 2229
Fragments arranged by size:
2783 2229 1545 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7033
PciI A'CATG_T
Cuts at: 4008 5932 4008
Size: 1924 5109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2791 3811 1108
Size: 1683 1020 4330
Fragments arranged by size:
4330 1683 1020
PfoI T'CCnGG_A
Cuts at: 2182 2182
Size: 7033
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PmlI CAC'GTG
Cuts at: 2709 2709
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 4603 4603
Size: 7033
PstI C_TGCA'G
Cuts at: 830 3030 3237 4385 830
Size: 2200 207 1148 3478
Fragments arranged by size:
3478 2200 1148 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 2176 2740 3524 2176
Size: 564 784 5685
Fragments arranged by size:
5685 784 564
SacI G_AGCT'C
Cuts at: 2054 3662 2054
Size: 1608 5425
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SapI GCTCTTCn'nnn_
Cuts at: 2575 3554 2575
Size: 979 6054
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 5281 6993 5281
Size: 1712 5321
SfcI C'TryA_G
Cuts at: 826 1045 3026 3233 4381 5516 6197 6388 826
Size: 219 1981 207 1148 1135 681 191 1471
Fragments arranged by size:
1981 1471 1148 1135 681 219 207 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 4360 1872
Size: 2488 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3004 5137 6038 6300 820
Size: 197 926 654 407 2133 901 262 1553
Fragments arranged by size:
2133 1553 926 901 654 407 262 197
SnaBI TAC'GTA
Cuts at: 493 4330 4824 493
Size: 3837 494 2702
Fragments arranged by size:
3837 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SspI AAT'ATT
Cuts at: 5 52 4957 5
Size: 47 4905 2081
Fragments arranged by size:
4905 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 2241 3074 3741 3810 4227 4472 513
Size: 552 1176 833 667 69 417 245 3074
Fragments arranged by size:
3074 1176 833 667 552 417 245 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3096 3242 5060 5077 5230 5415 5860
Size: 1255 1516 146 1818 17 153 185 445
Cuts at: 5860 325
Size: 1498
Fragments arranged by size:
1818 1516 1498 1255 445 185 153 146
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4011 5279 6991
Size: 274 80 33 51 1443 2034 1268 1712
Cuts at: 6991 96
Size: 138
Fragments arranged by size:
2034 1712 1443 1268 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3649 3681 6993 7025 3649
Size: 32 3312 32 3657
Fragments arranged by size:
3657 3312 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3680 1316
Size: 2364 4669
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3739 3739
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 4358 1870
Size: 2488 4545
XmnI GAAnn'nnTTC
Cuts at: 5162 5162
Size: 7033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3915 4841 276
Size: 53 83 186 936 2381 926 2468
Fragments arranged by size:
2468 2381 936 926 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI