Restriction map for
pFN21ASDA0955
(Circular) (Six-base) MAPSORT of: pFN21ASDA0955.seq Check: 7332 from: 1 to: 6022
pFN21ASDA0955 6022 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2493 3832 278
Size: 53 83 186 936 957 1339 2468
Fragments arranged by size:
2468 1339 957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3365 1937
Size: 1428 4594
Acc65I G'GTAC_C
Cuts at: 3343 3343
Size: 6022
AclI AA'CG_TT
Cuts at: 1563 4149 4522 1563
Size: 2586 373 3063
Fragments arranged by size:
3063 2586 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 3241 4085 5469 5737 1632
Size: 588 1021 844 1384 268 1917
Fragments arranged by size:
1917 1384 1021 844 588 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5825
AflIII A'CryG_T
Cuts at: 1203 4921 5898 1203
Size: 3718 977 1327
Fragments arranged by size:
3718 1327 977
AgeI A'CCGG_T
Cuts at: 4853 4853
Size: 6022
AhdI GACnn_n'nnGTC
Cuts at: 4751 4751
Size: 6022
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6022
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2627 1184
Size: 1443 4579
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 5990
AlwNI CAG_nnn'CTG
Cuts at: 2191 2359 2392 3765 5337 2191
Size: 168 33 1373 1572 2876
Fragments arranged by size:
2876 1572 1373 168 33
ApaLI G'TGCA_C
Cuts at: 4079 5235 4079
Size: 1156 4866
ApoI r'AATT_y
Cuts at: 1493 2898 2915 3310 3331 3557 3709 5818 1493
Size: 1405 17 395 21 226 152 2109 1697
Fragments arranged by size:
2109 1697 1405 395 226 152 21 17
AseI AT'TA_AT
Cuts at: 160 1032 4576 160
Size: 872 3544 1606
Fragments arranged by size:
3544 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
AvaI C'yCGr_G
Cuts at: 1870 1943 2550 3347 1870
Size: 73 607 797 4545
Fragments arranged by size:
4545 797 607 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2982 3015 2982
Size: 33 5989
BamHI G'GATC_C
Cuts at: 3352 3719 3352
Size: 367 5655
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3343 4798 618
Size: 325 217 627 1556 1455 1842
Fragments arranged by size:
1842 1556 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2891 3341 1391
Size: 1500 450 4072
Fragments arranged by size:
4072 1500 450
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4213 4247 1903
Size: 34 2276 34 3678
Fragments arranged by size:
3678 2276 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2983 3914 5124 2983
Size: 931 1210 3881
Fragments arranged by size:
3881 1210 931
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4633 136
Size: 107 122 71 631 605 2961 1525
Fragments arranged by size:
2961 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6017 6017
Size: 6022
BlpI GC'TnA_GC
Cuts at: 3212 3439 3212
Size: 227 5795
Bme1580I G_kGCm'C
Cuts at: 1790 4083 5239 1790
Size: 2293 1156 2573
Fragments arranged by size:
2573 2293 1156
BmgBI CAC'GTC
Cuts at: 1318 3250 1318
Size: 1932 4090
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3753 4711 442
Size: 416 53 83 399 24 2336 958 1753
Fragments arranged by size:
2336 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2368 2558 2913 2999 3113 4682 1239
Size: 1129 190 355 86 114 1569 2579
Fragments arranged by size:
2579 1569 1129 355 190 114 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2972 4147 5012 5310 2972
Size: 1175 865 298 3684
Fragments arranged by size:
3684 1175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4685 882
Size: 617 3186 2219
Fragments arranged by size:
3186 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3319 3813 493
Size: 711 2115 494 2702
Fragments arranged by size:
2702 2115 711 494
BsaBI GATnn'nnATC
Cuts at: 2603 6016 2603
Size: 3413 2609
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2490 2724 3829 4211
Size: 53 83 186 936 957 234 1105 382
Cuts at: 4211 4846 275
Size: 635 1451
Fragments arranged by size:
1451 1105 957 936 635 382 234 186
83 53
BsaWI w'CCGG_w
Cuts at: 4455 4853 5127 5274 5685 4455
Size: 398 274 147 411 4792
Fragments arranged by size:
4792 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 1178
Size: 30 165 30 1050 30 4717
Fragments arranged by size:
4717 1050 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 3030 1186
Size: 1261 583 4178
Fragments arranged by size:
4178 1261 583
BseYI C'CCAG_C
Cuts at: 2585 2717 2924 5225 5721 2585
Size: 132 207 2301 496 2886
Fragments arranged by size:
2886 2301 496 207 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3075 1163
Size: 573 1339 4110
Fragments arranged by size:
4110 1339 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3472 4233 5261 1540
Size: 275 186 1471 761 1028 2301
Fragments arranged by size:
2301 1471 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 3120 3255 4083 4168 5239 2237
Size: 883 135 828 85 1071 3020
Fragments arranged by size:
3020 1071 883 828 135 85
BsmI GAATG_Cn'
Cuts at: 3533 3626 3533
Size: 93 5929
BsmBI CGTCTCn'nnnn_
Cuts at: 2497 2497
Size: 6022
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2891 3120 3255 3341 4083 4168
Size: 399 447 654 229 135 86 742 85
Cuts at: 4168 5239 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 654 447 399 229 135
86 85
BspEI T'CCGG_A
Cuts at: 5685 5685
Size: 6022
BspHI T'CATG_A
Cuts at: 3911 5641 3911
Size: 1730 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 3377 844
Size: 1535 998 3489
Fragments arranged by size:
3489 1535 998
BsrBI CCG'CTC
Cuts at: 1394 1823 3909 1394
Size: 429 2086 3507
Fragments arranged by size:
3507 2086 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4517 4691 65
Size: 1183 3269 174 1396
Fragments arranged by size:
3269 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3078 4666 4853 1700
Size: 110 1268 1588 187 2869
Fragments arranged by size:
2869 1588 1268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6022
BssSI C'ACGA_G
Cuts at: 3287 4082 5094 3287
Size: 795 1012 4215
Fragments arranged by size:
4215 1012 795
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6022
BstBI TT'CG_AA
Cuts at: 2007 4826 2007
Size: 2819 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6022
BstYI r'GATC_y
Cuts at: 1972 3010 3352 3719 4104 4121 5573 5659 5671
Size: 1038 342 367 385 17 1452 86 12
Cuts at: 5671 6017 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 342 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2767 513
Size: 552 1702 3768
Fragments arranged by size:
3768 1702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5283
Fragments arranged by size:
5283 683 56
BtsI GCAGTG_nn'
Cuts at: 2262 2418 3538 4332 4352 2262
Size: 156 1120 794 20 3932
Fragments arranged by size:
3932 1120 794 156 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6022
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5987
DraI TTT'AAA
Cuts at: 3326 3326
Size: 6022
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5029 809
Size: 507 3713 1802
Fragments arranged by size:
3713 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3469 4358 5987
Size: 54 1425 50 275 18 1639 889 1629
Cuts at: 5987 8
Size: 43
Fragments arranged by size:
1639 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3469 1537
Size: 275 1657 4090
Fragments arranged by size:
4090 1657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 3260 3952 1353
Size: 756 1151 692 3423
Fragments arranged by size:
3423 1151 756 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4609 4983 5129 387
Size: 1064 138 3020 374 146 1280
Fragments arranged by size:
3020 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2368 2558 2913 2999 3113 3241
Size: 393 588 148 190 355 86 114 128
Cuts at: 3241 4085 4682 5469 5737 1239
Size: 844 597 787 268 1524
Fragments arranged by size:
1524 844 787 597 588 393 355 268
190 148 128 114 86
EcoNI CCTnn'n_nnAGG
Cuts at: 3048 3048
Size: 6022
EcoO109I rG'GnC_Cy
Cuts at: 2195 3103 3235 3277 2195
Size: 908 132 42 4940
Fragments arranged by size:
4940 908 132 42
EcoRI G'AATT_C
Cuts at: 3331 3709 3331
Size: 378 5644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 3297 2719
Size: 578 5444
FspI TGC'GCA
Cuts at: 4528 4528
Size: 6022
HaeII r_GCGC'y
Cuts at: 1678 5169 1678
Size: 3491 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2656 2688 4663
Size: 32 135 32 521 32 531 32 1975
Cuts at: 4663 4695 4737 4769 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1975 531 521 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2074 3366 3612 669
Size: 988 281 136 1292 246 3079
Fragments arranged by size:
3079 1292 988 281 246 136
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6022
HpaI GTT'AAC
Cuts at: 3612 3612
Size: 6022
KpnI G_GTAC'C
Cuts at: 3347 3347
Size: 6022
MfeI C'AATT_G
Cuts at: 3621 3621
Size: 6022
MluI A'CGCG_T
Cuts at: 5898 5898
Size: 6022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2679 5136 5320 1305
Size: 1374 2457 184 2007
Fragments arranged by size:
2457 2007 1374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4200
Fragments arranged by size:
4200 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3980 4339 4498 518
Size: 648 2814 359 159 2042
Fragments arranged by size:
2814 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3438 4115 5263 5508 1606
Size: 1832 677 1148 245 2120
Fragments arranged by size:
2120 1832 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2767 513
Size: 552 1702 3768
Fragments arranged by size:
3768 1702 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6022
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6022
NotI GC'GGCC_GC
Cuts at: 3469 3469
Size: 6022
NspI r_CATG'y
Cuts at: 1132 1229 3380 4925 1132
Size: 97 2151 1545 2229
Fragments arranged by size:
2229 2151 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6022
PciI A'CATG_T
Cuts at: 4921 4921
Size: 6022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6022
PfoI T'CCnGG_A
Cuts at: 2345 2535 2574 3129 2345
Size: 190 39 555 5238
Fragments arranged by size:
5238 555 190 39
PmeI GTTT'AAAC
Cuts at: 3326 3326
Size: 6022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4091 4123 5604 5636 4091
Size: 32 1481 32 4477
Fragments arranged by size:
4477 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3103 3235 3277 3103
Size: 132 42 5848
Fragments arranged by size:
5848 132 42
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6022
PsiI TTA'TAA
Cuts at: 2175 3592 2175
Size: 1417 4605
PstI C_TGCA'G
Cuts at: 830 2376 2425 2784 3374 830
Size: 1546 49 359 590 3478
Fragments arranged by size:
3478 1546 590 359 49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6022
SalI G'TCGA_C
Cuts at: 1936 3364 1936
Size: 1428 4594
SanDI GG'GwC_CC
Cuts at: 3277 3277
Size: 6022
SapI GCTCTTCn'nnn_
Cuts at: 3260 3260
Size: 6022
SbfI CC_TGCA'GG
Cuts at: 3374 3374
Size: 6022
ScaI AGT'ACT
Cuts at: 4270 5982 4270
Size: 1712 4310
SfcI C'TryA_G
Cuts at: 826 1045 2372 2421 2780 2881 3370 4505 5186
Size: 219 1327 49 359 101 489 1135 681
Cuts at: 5186 5377 826
Size: 191 1471
Fragments arranged by size:
1471 1327 1135 681 489 359 219 191
101 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6022
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6022
SmaI CCC'GGG
Cuts at: 1872 2552 3349 1872
Size: 680 797 4545
Fragments arranged by size:
4545 797 680
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2951 4126 5027 5289 820
Size: 197 926 1008 1175 901 262 1553
Fragments arranged by size:
1553 1175 1008 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3319 3813 493
Size: 2826 494 2702
Fragments arranged by size:
2826 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6022
SphI G_CATG'C
Cuts at: 1132 3380 1132
Size: 2248 3774
SspI AAT'ATT
Cuts at: 5 52 3946 5
Size: 47 3894 2081
Fragments arranged by size:
3894 2081 47
StuI AGG'CCT
Cuts at: 1517 2295 3206 1517
Size: 778 911 4333
Fragments arranged by size:
4333 911 778
StyI C'CwwG_G
Cuts at: 513 1065 2476 2767 2862 3461 513
Size: 552 1411 291 95 599 3074
Fragments arranged by size:
3074 1411 599 552 291 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4049 4066 4219 4404 4849 325
Size: 1255 2469 17 153 185 445 1498
Fragments arranged by size:
2469 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4268 5980 96
Size: 274 80 33 51 1443 2291 1712 138
Fragments arranged by size:
2291 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2453 2485 2608 2640 5982 6014 2453
Size: 32 123 32 3342 32 2461
Fragments arranged by size:
3342 2461 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6022
XbaI T'CTAG_A
Cuts at: 3358 3358
Size: 6022
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6022
XmaI C'CCGG_G
Cuts at: 1870 2550 3347 1870
Size: 680 797 4545
Fragments arranged by size:
4545 797 680
XmnI GAAnn'nnTTC
Cuts at: 2943 4151 2943
Size: 1208 4814
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2491 3830 276
Size: 53 83 186 936 957 1339 2468
Fragments arranged by size:
2468 1339 957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI