Restriction map for
pFN21ASDA0952
(Circular) (Six-base) MAPSORT of: pFN21ASDA0952.seq Check: 8952 from: 1 to: 6295
pFN21ASDA0952 6295 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2518 3077 3638 1937
Size: 581 559 561 4594
Fragments arranged by size:
4594 581 561 559
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2922 3086 4358 5742 6010 1632
Size: 1290 164 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1290 1272 268 164
AfeI AGC'GCT
Cuts at: 3143 3143
Size: 6295
AflII C'TTAA_G
Cuts at: 820 1017 3305 820
Size: 197 2288 3810
Fragments arranged by size:
3810 2288 197
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2171 1184
Size: 987 5308
AlwNI CAG_nnn'CTG
Cuts at: 2655 4038 5610 2655
Size: 1383 1572 3340
Fragments arranged by size:
3340 1572 1383
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 3604 3830 3982 6091 1493
Size: 2111 226 152 2109 1697
Fragments arranged by size:
2111 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 3620 1870
Size: 73 1677 4545
Fragments arranged by size:
4545 1677 73
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3616 5071 618
Size: 325 217 627 1829 1455 1842
Fragments arranged by size:
1842 1829 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2462 3614 1391
Size: 1071 1152 4072
Fragments arranged by size:
4072 1152 1071
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2195 928
Size: 971 296 5028
Fragments arranged by size:
5028 971 296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2519 2553 2993 3027 4486 4520 1903
Size: 34 582 34 440 34 1459 34 3678
Fragments arranged by size:
3678 1459 582 440 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 136
Size: 107 122 71 631 605 3234 1525
Fragments arranged by size:
3234 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2923 6290 2923
Size: 3367 2928
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 4356 5512 1790
Size: 2566 1156 2573
Fragments arranged by size:
2573 2566 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2905 3389 3555
Size: 416 53 83 399 24 1488 484 166
Cuts at: 3555 4026 4984 442
Size: 471 958 1753
Fragments arranged by size:
1753 1488 958 484 471 416 399 166
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2230 2690 3250 4955 1239
Size: 991 460 560 1705 2579
Fragments arranged by size:
2579 1705 991 560 460
Bpu10I CC'TnA_GC
Cuts at: 3096 3096
Size: 6295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2774 3358 4420 5285 5583 2774
Size: 584 1062 865 298 3486
Fragments arranged by size:
3486 1062 865 584 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 3047 4958 882
Size: 617 1167 381 1911 2219
Fragments arranged by size:
2219 1911 1167 617 381
BsaAI yAC'GTr
Cuts at: 493 1204 3592 4086 493
Size: 711 2388 494 2702
Fragments arranged by size:
2702 2388 711 494
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 4484 5119 275
Size: 53 83 186 936 2569 382 635 1451
Fragments arranged by size:
2569 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6070
Fragments arranged by size:
6070 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2696 2744 3514 1186
Size: 1510 48 770 3967
Fragments arranged by size:
3967 1510 770 48
BseYI C'CCAG_C
Cuts at: 2745 2754 3138 5498 5994 2745
Size: 9 384 2360 496 3046
Fragments arranged by size:
3046 2360 496 384 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3009 3419 1163
Size: 573 1273 410 4039
Fragments arranged by size:
4039 1273 573 410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2462 2706 3524 4356 4441 5512 2462
Size: 244 818 832 85 1071 3245
Fragments arranged by size:
3245 1071 832 818 244 85
BsmI GAATG_Cn'
Cuts at: 2698 2863 2915 3806 3899 2698
Size: 165 52 891 93 5094
Fragments arranged by size:
5094 891 165 93 52
BsmBI CGTCTCn'nnnn_
Cuts at: 2075 2075
Size: 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2462 2706 3524 3614 4356 4441 5512
Size: 399 672 244 818 90 742 85 1071
Cuts at: 5512 1391
Size: 2174
Fragments arranged by size:
2174 1071 818 742 672 399 244 90
85
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2542 3650 844
Size: 1698 1108 3489
Fragments arranged by size:
3489 1698 1108
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3240 3454 4790 4964 65
Size: 1183 1992 214 1336 174 1396
Fragments arranged by size:
1992 1396 1336 1183 214 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2386 96
Size: 2290 4005
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3020 794
Size: 2226 4069
BstBI TT'CG_AA
Cuts at: 2007 5099 2007
Size: 3092 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 2923 3564 3625 3992 4377 4394 5846 5932
Size: 951 641 61 367 385 17 1452 86
Cuts at: 5932 5944 6290 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 951 641 385 367 346 86
61 17 12
BstZ17I GTA'TAC
Cuts at: 2519 3078 2519
Size: 559 5736
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4786
Fragments arranged by size:
4786 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2369 2489 487
Size: 683 56 1143 120 4293
Fragments arranged by size:
4293 1143 683 120 56
BtsI GCAGTG_nn'
Cuts at: 3811 4605 4625 3811
Size: 794 20 5481
Fragments arranged by size:
5481 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DraIII CAC_nnn'GTG
Cuts at: 2411 2411
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2604 3742 4631
Size: 54 1425 50 275 18 774 1138 889
Cuts at: 4631 6260 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1138 889 774 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4225 1353
Size: 2872 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2460 2460
Size: 6295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2690 2922 3086 3250 4358 4955
Size: 393 598 460 232 164 164 1108 597
Cuts at: 4955 5742 6010 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1108 787 598 597 460 393 268
232 164 164
EcoNI CCTnn'n_nnAGG
Cuts at: 2726 2726
Size: 6295
EcoO109I rG'GnC_Cy
Cuts at: 3127 3127
Size: 6295
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 3145 3190 5442 1678
Size: 1467 45 2252 2531
Fragments arranged by size:
2531 2252 1467 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3549 3581 4936 4968 5010
Size: 32 135 32 1977 32 1355 32 42
Cuts at: 5010 5042 1373
Size: 32 2626
Fragments arranged by size:
2626 1977 1355 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6295
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2606 10
Size: 54 1425 343 774 3699
Fragments arranged by size:
3699 1425 774 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 4253 4612 4771 518
Size: 648 1273 1814 359 159 2042
Fragments arranged by size:
2042 1814 1273 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3270 3711 4388 5536 5781 1606
Size: 1664 441 677 1148 245 2120
Fragments arranged by size:
2120 1664 1148 677 441 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4786
Fragments arranged by size:
4786 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 3653 5198 1132
Size: 97 2424 1545 2229
Fragments arranged by size:
2424 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2411 1108
Size: 1303 4992
PfoI T'CCnGG_A
Cuts at: 2667 2667
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PstI C_TGCA'G
Cuts at: 830 3085 3283 3647 830
Size: 2255 198 364 3478
Fragments arranged by size:
3478 2255 364 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 3270 3270
Size: 6295
SacI G_AGCT'C
Cuts at: 2462 2462
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 3347 4543 6255 3347
Size: 1196 1712 3387
Fragments arranged by size:
3387 1712 1196
SexAI A'CCwGG_T
Cuts at: 2407 2407
Size: 6295
SfcI C'TryA_G
Cuts at: 826 1045 3081 3279 3643 4778 5459 5650 826
Size: 219 2036 198 364 1135 681 191 1471
Fragments arranged by size:
2036 1471 1135 681 364 219 198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2789 3305 3337 4399 5300 5562
Size: 197 926 846 516 32 1062 901 262
Cuts at: 5562 820
Size: 1553
Fragments arranged by size:
1553 1062 926 901 846 516 262 197
32
SnaBI TAC'GTA
Cuts at: 493 3592 4086 493
Size: 3099 494 2702
Fragments arranged by size:
3099 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 2022 3734 513
Size: 552 957 1712 3074
Fragments arranged by size:
3074 1712 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4322 4339 4492 4677 5122 325
Size: 1255 2742 17 153 185 445 1498
Fragments arranged by size:
2742 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 2628 3345
Size: 274 80 33 51 1443 409 242 717
Cuts at: 3345 4541 6253 96
Size: 1196 1712 138
Fragments arranged by size:
1712 1443 1196 717 409 274 242 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6255 6287 6255
Size: 32 6263
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2869 3436 2869
Size: 567 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 2835 4424 2835
Size: 1589 4706
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI Bsu36I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI