Restriction map for
pFN21ASDA0948
(Circular) (Six-base) MAPSORT of: pFN21ASDA0948.seq Check: 8747 from: 1 to: 6292
pFN21ASDA0948 6292 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2224 2908 2224
Size: 684 5608
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3489 4102 278
Size: 53 83 186 936 1953 613 2468
Fragments arranged by size:
2468 1953 936 613 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 2453 3613 2453
Size: 1160 5132
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2845 3212 4355 5739 6007 1632
Size: 1213 367 1143 1384 268 1917
Fragments arranged by size:
1917 1384 1213 1143 367 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 2693 1166
Size: 1527 4765
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3440 1184
Size: 2256 4036
AlwNI CAG_nnn'CTG
Cuts at: 2629 3052 4035 5607 2629
Size: 423 983 1572 3314
Fragments arranged by size:
3314 1572 983 423
ApaI G_GGCC'C
Cuts at: 2938 3138 2938
Size: 200 6092
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 2993 3601 3827 3979 6088 1493
Size: 1500 608 226 152 2109 1697
Fragments arranged by size:
2109 1697 1500 608 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3089 3097 3227 3617 1870
Size: 73 1146 8 130 390 4545
Fragments arranged by size:
4545 1146 390 130 73 8
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 3316 3533 3613 5068
Size: 325 217 627 666 863 217 80 1455
Cuts at: 5068 618
Size: 1842
Fragments arranged by size:
1842 1455 863 666 627 325 217 217
80
BanII G_rGCy'C
Cuts at: 1391 2277 2938 3138 3611 1391
Size: 886 661 200 473 4072
Fragments arranged by size:
4072 886 661 473 200
BbeI G_GCGC'C
Cuts at: 3537 3537
Size: 6292
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2757 928
Size: 971 858 4463
Fragments arranged by size:
4463 971 858
BbvCI CC'TCA_GC
Cuts at: 2146 3139 3264 3570 2146
Size: 993 125 306 4868
Fragments arranged by size:
4868 993 306 125
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2034 2068 4483 4517 1903
Size: 34 97 34 2415 34 3678
Fragments arranged by size:
3678 2415 97 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2582 4903 136
Size: 107 122 71 631 605 910 2321 1525
Fragments arranged by size:
2321 1525 910 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 2637 3709 2637
Size: 1072 5220
Bme1580I G_kGCm'C
Cuts at: 1790 2938 3138 4353 5509 1790
Size: 1148 200 1215 1156 2573
Fragments arranged by size:
2573 1215 1156 1148 200
BmgBI CAC'GTC
Cuts at: 1318 2761 1318
Size: 1443 4849
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4023 4981
Size: 416 53 83 399 24 1931 675 958
Cuts at: 4981 442
Size: 1753
Fragments arranged by size:
1931 1753 958 675 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3377 1056
Size: 2321 3971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2079 2300 2924 3101 3342 4952 1239
Size: 840 221 624 177 241 1610 2579
Fragments arranged by size:
2579 1610 840 624 241 221 177
Bpu10I CC'TnA_GC
Cuts at: 2146 3139 3264 3570 2146
Size: 993 125 306 4868
Fragments arranged by size:
4868 993 306 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3497 3527 4417 5282 5580 2543
Size: 954 30 890 865 298 3255
Fragments arranged by size:
3255 954 890 865 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4955 882
Size: 617 3456 2219
Fragments arranged by size:
3456 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2052 3589 4083 493
Size: 711 848 1537 494 2702
Fragments arranged by size:
2702 1537 848 711 494
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3486 3534 4099 4481
Size: 53 83 186 936 1953 48 565 382
Cuts at: 4481 5116 275
Size: 635 1451
Fragments arranged by size:
1953 1451 936 635 565 382 186 83
53 48
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 1178
Size: 30 165 30 1101 30 4936
Fragments arranged by size:
4936 1101 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 3587 1186
Size: 1099 1302 3891
Fragments arranged by size:
3891 1302 1099
BseYI C'CCAG_C
Cuts at: 2470 2881 3202 3249 3330 3427 5495 5991 2470
Size: 411 321 47 81 97 2068 496 2771
Fragments arranged by size:
2771 2068 496 411 321 97 81 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2821 1163
Size: 573 1085 4634
Fragments arranged by size:
4634 1085 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 4353 4438 5509 2277
Size: 2076 85 1071 3060
Fragments arranged by size:
3060 2076 1071 85
BsmI GAATG_Cn'
Cuts at: 3803 3896 3803
Size: 93 6199
BsmBI CGTCTCn'nnnn_
Cuts at: 3179 3179
Size: 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2938 3138 3611 4353 4438 5509
Size: 399 487 661 200 473 742 85 1071
Cuts at: 5509 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 661 487 473 399 200
85
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 2703 4181 5911 2703
Size: 1478 1730 3084
Fragments arranged by size:
3084 1730 1478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 2908 3647 844
Size: 1380 684 739 3489
Fragments arranged by size:
3489 1380 739 684
BsrBI CCG'CTC
Cuts at: 1394 1823 4179 1394
Size: 429 2356 3507
Fragments arranged by size:
3507 2356 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2331 4787 4961 65
Size: 1183 1083 2456 174 1396
Fragments arranged by size:
2456 1396 1183 1083 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2191 4936 5123 1700
Size: 110 381 2745 187 2869
Fragments arranged by size:
2869 2745 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2629 794
Size: 1835 4457
BstBI TT'CG_AA
Cuts at: 2007 5096 2007
Size: 3089 3203
BstEII G'GTnAC_C
Cuts at: 1184 3157 1184
Size: 1973 4319
BstXI CCAn_nnnn'nTGG
Cuts at: 3338 3338
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287
Size: 1650 367 385 17 1452 86 12 346
Cuts at: 6287 1972
Size: 1977
Fragments arranged by size:
1977 1650 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2088 513
Size: 552 957 66 4717
Fragments arranged by size:
4717 957 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2059 487
Size: 683 56 833 4720
Fragments arranged by size:
4720 833 683 56
BtsI GCAGTG_nn'
Cuts at: 3069 3808 4602 4622 3069
Size: 739 794 20 4739
Fragments arranged by size:
4739 794 739 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DraIII CAC_nnn'GTG
Cuts at: 3460 3460
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2759 5299 809
Size: 507 1443 2540 1802
Fragments arranged by size:
2540 1802 1443 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 3234 3579
Size: 54 1425 50 275 18 271 1133 345
Cuts at: 3579 3739 4628 6257 8
Size: 160 889 1629 43
Fragments arranged by size:
1629 1425 1133 889 345 275 271 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4222 1353
Size: 2869 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 6292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2300 2845 2924 3101 3212 3342
Size: 393 447 221 545 79 177 111 130
Cuts at: 3342 4355 4952 5739 6007 1239
Size: 1013 597 787 268 1524
Fragments arranged by size:
1524 1013 787 597 545 447 393 268
221 177 130 111 79
EcoNI CCTnn'n_nnAGG
Cuts at: 2606 3170 2606
Size: 564 5728
EcoO109I rG'GnC_Cy
Cuts at: 2080 2935 3101 3135 2080
Size: 855 166 34 5237
Fragments arranged by size:
5237 855 166 34
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FspI TGC'GCA
Cuts at: 3557 4798 3557
Size: 1241 5051
HaeII r_GCGC'y
Cuts at: 1678 3213 3537 5439 1678
Size: 1535 324 1902 2531
Fragments arranged by size:
2531 1902 1535 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2504 2536 4933 4965 5007
Size: 32 135 32 932 32 2397 32 42
Cuts at: 5007 5039 1373
Size: 32 2626
Fragments arranged by size:
2626 2397 932 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KasI G'GCGC_C
Cuts at: 3533 3533
Size: 6292
KpnI G_GTAC'C
Cuts at: 2457 3617 2457
Size: 1160 5132
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2645 5406 5590 1305
Size: 1340 2761 184 2007
Fragments arranged by size:
2761 2007 1340 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3236 3581 10
Size: 54 1425 343 1404 345 2721
Fragments arranged by size:
2721 1425 1404 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 4250 4609 4768 518
Size: 648 1527 1557 359 159 2042
Fragments arranged by size:
2042 1557 1527 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2541 3708 4385 5533 5778 1606
Size: 935 1167 677 1148 245 2120
Fragments arranged by size:
2120 1167 1148 935 677 245
NarI GG'CG_CC
Cuts at: 3534 3534
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 2022 2088 513
Size: 552 957 66 4717
Fragments arranged by size:
4717 957 552 66
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 3373 1052
Size: 2321 3971
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 2551 3650 5195 1132
Size: 97 1322 1099 1545 2229
Fragments arranged by size:
2229 1545 1322 1099 97
PasI CC'CwG_GG
Cuts at: 1348 3131 1348
Size: 1783 4509
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6292
PfoI T'CCnGG_A
Cuts at: 2277 2277
Size: 6292
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PmlI CAC'GTG
Cuts at: 2052 2052
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 4361 4393 5874 5906 2840
Size: 32 1489 32 1481 32 3226
Fragments arranged by size:
3226 1489 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3101 3101
Size: 6292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PspOMI G'GGCC_C
Cuts at: 2934 3134 2934
Size: 200 6092
PspXI vC'TCGA_Gb
Cuts at: 3227 3227
Size: 6292
PstI C_TGCA'G
Cuts at: 830 2520 2919 2952 3183 3469 3644 830
Size: 1690 399 33 231 286 175 3478
Fragments arranged by size:
3478 1690 399 286 231 175 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SanDI GG'GwC_CC
Cuts at: 3101 3101
Size: 6292
SbfI CC_TGCA'GG
Cuts at: 2919 3644 2919
Size: 725 5567
ScaI AGT'ACT
Cuts at: 3095 4540 6252 3095
Size: 1445 1712 3135
Fragments arranged by size:
3135 1712 1445
SexAI A'CCwGG_T
Cuts at: 2034 2925 2034
Size: 891 5401
SfcI C'TryA_G
Cuts at: 826 1045 2516 2915 2948 3179 3465 3640 4775
Size: 219 1471 399 33 231 286 175 1135
Cuts at: 4775 5456 5647 826
Size: 681 191 1471
Fragments arranged by size:
1471 1471 1135 681 399 286 231 219
191 175 33
SfoI GGC'GCC
Cuts at: 3535 3535
Size: 6292
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3227 3512 3542 4396 5297
Size: 197 926 615 669 285 30 854 901
Cuts at: 5297 5559 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 854 669 615 285 262
197 30
SnaBI TAC'GTA
Cuts at: 493 3589 4083 493
Size: 3096 494 2702
Fragments arranged by size:
3096 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 2551 3650 1132
Size: 1419 1099 3774
Fragments arranged by size:
3774 1419 1099
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 2504 2901 1517
Size: 987 397 4908
Fragments arranged by size:
4908 987 397
StyI C'CwwG_G
Cuts at: 513 1065 2022 2088 2499 2508 3731 513
Size: 552 957 66 411 9 1223 3074
Fragments arranged by size:
3074 1223 957 552 411 66 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 2431 3241 4319 4336 4489 4674
Size: 1255 593 258 810 1078 17 153 185
Cuts at: 4674 5119 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1078 810 593 445 258 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 3093 4538
Size: 274 80 33 51 1443 585 531 1445
Cuts at: 4538 6250 96
Size: 1712 138
Fragments arranged by size:
1712 1445 1443 585 531 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2915 2947 6252 6284 2915
Size: 32 3305 32 2923
Fragments arranged by size:
3305 2923 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3498 3076
Size: 422 5870
XhoI C'TCGA_G
Cuts at: 1943 3227 1943
Size: 1284 5008
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 3041 4421 3041
Size: 1380 4912
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3487 4100 276
Size: 53 83 186 936 1953 613 2468
Fragments arranged by size:
2468 1953 936 613 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PsrI PvuII RsrII SacII SapI SfiI
SrfI SwaI