Restriction map for
pFN21ASDA0945
(Circular) (Six-base) MAPSORT of: pFN21ASDA0945.seq Check: 8655 from: 1 to: 7066
pFN21ASDA0945 7066 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2970 3037 3565 2970
Size: 67 528 6471
Fragments arranged by size:
6471 528 67
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4876 278
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4409 1937
Size: 2472 4594
Acc65I G'GTAC_C
Cuts at: 3793 4387 3793
Size: 594 6472
AclI AA'CG_TT
Cuts at: 1563 5193 5566 1563
Size: 3630 373 3063
Fragments arranged by size:
3630 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2415 3014 3566 5129 6513 6781 1632
Size: 783 599 552 1563 1384 268 1917
Fragments arranged by size:
1917 1563 1384 783 599 552 268
AflII C'TTAA_G
Cuts at: 820 1017 4346 820
Size: 197 3329 3540
Fragments arranged by size:
3540 3329 197
AflIII A'CryG_T
Cuts at: 1203 3739 5965 6942 1203
Size: 2536 2226 977 1327
Fragments arranged by size:
2536 2226 1327 977
AgeI A'CCGG_T
Cuts at: 5897 5897
Size: 7066
AhdI GACnn_n'nnGTC
Cuts at: 5795 5795
Size: 7066
AleI CACnn'nnGTG
Cuts at: 1166 3356 1166
Size: 2190 4876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2255 3472 1184
Size: 1071 1217 4778
Fragments arranged by size:
4778 1217 1071
AlwNI CAG_nnn'CTG
Cuts at: 3046 3245 3375 4176 4809 6381 3046
Size: 199 130 801 633 1572 3731
Fragments arranged by size:
3731 1572 801 633 199 130
ApaLI G'TGCA_C
Cuts at: 5123 6279 5123
Size: 1156 5910
ApoI r'AATT_y
Cuts at: 1493 3479 4375 4601 4753 6862 1493
Size: 1986 896 226 152 2109 1697
Fragments arranged by size:
2109 1986 1697 896 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5620 160
Size: 872 4588 1606
Fragments arranged by size:
4588 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 2755 3986 4391 1870
Size: 73 204 608 1231 405 4545
Fragments arranged by size:
4545 1231 608 405 204 73
BamHI G'GATC_C
Cuts at: 2470 4396 4763 2470
Size: 1926 367 4773
Fragments arranged by size:
4773 1926 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2373 2447 3009 3296
Size: 325 217 627 385 201 74 562 287
Cuts at: 3296 3534 3793 4387 5842 618
Size: 238 259 594 1455 1842
Fragments arranged by size:
1842 1455 627 594 562 385 325 287
259 238 217 201 74
BanII G_rGCy'C
Cuts at: 1391 2088 2156 2739 2942 3141 3267 3284 3326
Size: 697 68 583 203 199 126 17 42
Cuts at: 3326 3570 3603 4074 4385 1391
Size: 244 33 471 311 4072
Fragments arranged by size:
4072 697 583 471 311 244 203 199
126 68 42 33 17
BbeI G_GCGC'C
Cuts at: 2176 2377 3013 2176
Size: 201 636 6229
Fragments arranged by size:
6229 636 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 2349 928
Size: 971 207 243 5645
Fragments arranged by size:
5645 971 243 207
BbvCI CC'TCA_GC
Cuts at: 3072 3072
Size: 7066
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3268 3302 5257 5291 1903
Size: 34 1331 34 1955 34 3678
Fragments arranged by size:
3678 1955 1331 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4958 6168 4958
Size: 1210 5856
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7066
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2073 2445 2767
Size: 107 122 71 631 605 401 372 322
Cuts at: 2767 2841 5677 136
Size: 74 2836 1525
Fragments arranged by size:
2836 1525 631 605 401 372 322 122
107 74 71
BglII A'GATC_T
Cuts at: 3963 7061 3963
Size: 3098 3968
BlpI GC'TnA_GC
Cuts at: 4483 4483
Size: 7066
Bme1580I G_kGCm'C
Cuts at: 1790 2707 3291 3539 3626 5127 6283 1790
Size: 917 584 248 87 1501 1156 2573
Fragments arranged by size:
2573 1501 1156 917 584 248 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2514 3233 3540
Size: 416 53 83 399 24 1097 719 307
Cuts at: 3540 4351 4797 5755 442
Size: 811 446 958 1753
Fragments arranged by size:
1753 1097 958 811 719 446 416 399
307 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2640 1056
Size: 1584 5482
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2946 2946
Size: 7066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2438 2559 2954 3375 3661 5726 1239
Size: 1034 165 121 395 421 286 2065 2579
Fragments arranged by size:
2579 2065 1034 421 395 286 165 121
Bpu10I CC'TnA_GC
Cuts at: 2921 3036 3072 3135 4032 4293 2921
Size: 115 36 63 897 261 5694
Fragments arranged by size:
5694 897 261 115 63 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5191 6056 6354 5191
Size: 865 298 5903
Fragments arranged by size:
5903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2607 5729 882
Size: 617 1108 3122 2219
Fragments arranged by size:
3122 2219 1108 617
BsaAI yAC'GTr
Cuts at: 493 1204 3740 4363 4857 493
Size: 711 2536 623 494 2702
Fragments arranged by size:
2702 2536 711 623 494
BsaBI GATnn'nnATC
Cuts at: 7060 7060
Size: 7066
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2173 2374 3010 4873
Size: 53 83 186 936 640 201 636 1863
Cuts at: 4873 5255 5890 275
Size: 382 635 1451
Fragments arranged by size:
1863 1451 936 640 636 635 382 201
186 83 53
BsaWI w'CCGG_w
Cuts at: 5499 5897 6171 6318 6729 5499
Size: 398 274 147 411 5836
Fragments arranged by size:
5836 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6841
Fragments arranged by size:
6841 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2509 2568 3035 3257 3272 3316 4148 1186
Size: 1323 59 467 222 15 44 832 4104
Fragments arranged by size:
4104 1323 832 467 222 59 44 15
BseYI C'CCAG_C
Cuts at: 2321 2358 2539 2617 2740 3505 3644 3789 4058
Size: 37 181 78 123 765 139 145 269
Cuts at: 4058 4211 6269 6765 2321
Size: 153 2058 496 2622
Fragments arranged by size:
2622 2058 765 496 269 181 153 145
139 123 78 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2298 3129 3231 1163
Size: 573 562 831 102 4998
Fragments arranged by size:
4998 831 573 562 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4516 5277 6305 1540
Size: 275 186 2515 761 1028 2301
Fragments arranged by size:
2515 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3141 3284 4038 5127 5212 6283 3141
Size: 143 754 1089 85 1071 3924
Fragments arranged by size:
3924 1089 1071 754 143 85
BsmI GAATG_Cn'
Cuts at: 4577 4670 4577
Size: 93 6973
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2156 2707 2739 2942 3141 3267
Size: 399 298 68 551 32 203 199 126
Cuts at: 3267 3284 3291 3326 3539 3570 3603 3626 4038
Size: 17 7 35 213 31 33 23 412
Cuts at: 4038 4074 4385 5127 5212 6283 1391
Size: 36 311 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 551 412 399 311 298
213 203 199 126 85 68 36 35
33 32 31 23 17 7
BspEI T'CCGG_A
Cuts at: 6729 6729
Size: 7066
BspHI T'CATG_A
Cuts at: 4955 6685 4955
Size: 1730 5336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2418 2970 3037 3565 4161 4421 844
Size: 1472 102 552 67 528 596 260 3489
Fragments arranged by size:
3489 1472 596 552 528 260 102 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2677 3880 4953 1394
Size: 429 854 1203 1073 3507
Fragments arranged by size:
3507 1203 1073 854 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5561 5735 65
Size: 1183 4313 174 1396
Fragments arranged by size:
4313 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2264 5710 5897 1700
Size: 110 454 3446 187 2869
Fragments arranged by size:
3446 2869 454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7066
BssSI C'ACGA_G
Cuts at: 2700 3254 4067 5126 6138 2700
Size: 554 813 1059 1012 3628
Fragments arranged by size:
3628 1059 1012 813 554
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3046 794
Size: 2252 4814
BstBI TT'CG_AA
Cuts at: 2007 5870 2007
Size: 3863 3203
BstEII G'GTnAC_C
Cuts at: 1184 2159 1184
Size: 975 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 2440 2632 2844 2440
Size: 192 212 6662
Fragments arranged by size:
6662 212 192
BstYI r'GATC_y
Cuts at: 1972 2470 3461 3963 4396 4763 5148 5165 6617
Size: 498 991 502 433 367 385 17 1452
Cuts at: 6617 6703 6715 7061 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 991 502 498 433 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3222 3292 3222
Size: 70 6996
BtgI C'CryG_G
Cuts at: 513 1065 2867 4182 4333 513
Size: 552 1802 1315 151 3246
Fragments arranged by size:
3246 1802 1315 552 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4080 487
Size: 683 56 2854 3473
Fragments arranged by size:
3473 2854 683 56
BtsI GCAGTG_nn'
Cuts at: 3141 3194 3690 4582 5376 5396 3141
Size: 53 496 892 794 20 4811
Fragments arranged by size:
4811 892 794 496 53 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7031
DraI TTT'AAA
Cuts at: 4370 4370
Size: 7066
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6073 809
Size: 507 4757 1802
Fragments arranged by size:
4757 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2262 2870
Size: 54 1425 50 275 18 244 188 608
Cuts at: 2870 3172 3499 3957 4185 4513 5402 7031 8
Size: 302 327 458 228 328 889 1629 43
Fragments arranged by size:
1629 1425 889 608 458 328 327 302
275 244 228 188 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4513 1537
Size: 275 2701 4090
Fragments arranged by size:
4090 2701 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4996 1353
Size: 3643 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4319 5653 6027 6173 387
Size: 1064 138 2730 1334 374 146 1280
Fragments arranged by size:
2730 1334 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3139 3282 3139
Size: 143 6923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2415 2438 2559 2954 3014 3375
Size: 393 641 142 23 121 395 60 361
Cuts at: 3375 3566 3661 5129 5726 6513 6781 1239
Size: 191 95 1468 597 787 268 1524
Fragments arranged by size:
1524 1468 787 641 597 395 393 361
268 191 142 121 95 60 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2924 2924
Size: 7066
EcoO109I rG'GnC_Cy
Cuts at: 2545 2566 2597 2752 2545
Size: 21 31 155 6859
Fragments arranged by size:
6859 155 31 21
EcoRI G'AATT_C
Cuts at: 4375 4753 4375
Size: 378 6688
FspI TGC'GCA
Cuts at: 3668 5572 3668
Size: 1904 5162
HaeII r_GCGC'y
Cuts at: 1678 2176 2203 2377 2728 3013 3636 3751 4195
Size: 498 27 174 351 285 623 115 444
Cuts at: 4195 6213 1678
Size: 2018 2531
Fragments arranged by size:
2531 2018 623 498 444 351 285 174
115 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2143 2175 2711
Size: 32 135 32 445 32 94 32 536
Cuts at: 2711 2743 5707 5739 5781 5813 1373
Size: 32 2964 32 42 32 2626
Fragments arranged by size:
2964 2626 536 445 135 94 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4410 4656 669
Size: 988 281 2472 246 3079
Fragments arranged by size:
3079 2472 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7066
HpaI GTT'AAC
Cuts at: 4656 4656
Size: 7066
KasI G'GCGC_C
Cuts at: 2172 2373 3009 2172
Size: 201 636 6229
Fragments arranged by size:
6229 636 201
KpnI G_GTAC'C
Cuts at: 3797 4391 3797
Size: 594 6472
MfeI C'AATT_G
Cuts at: 4665 4665
Size: 7066
MluI A'CGCG_T
Cuts at: 6942 6942
Size: 7066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3456 6180 6364 1305
Size: 2151 2724 184 2007
Fragments arranged by size:
2724 2151 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2872 3174 3501 3959 4187
Size: 54 1425 343 1040 302 327 458 228
Cuts at: 4187 10
Size: 2889
Fragments arranged by size:
2889 1425 1040 458 343 327 302 228
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3356 4320 5024 5383 5542 518
Size: 648 2190 964 704 359 159 2042
Fragments arranged by size:
2190 2042 964 704 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2651 3076 3085 3648 3681 4482 5159 6307
Size: 1045 425 9 563 33 801 677 1148
Cuts at: 6307 6552 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1045 801 677 563 425 245
33 9
NarI GG'CG_CC
Cuts at: 2173 2374 3010 2173
Size: 201 636 6229
Fragments arranged by size:
6229 636 201
NcoI C'CATG_G
Cuts at: 513 1065 2867 4182 513
Size: 552 1802 1315 3397
Fragments arranged by size:
3397 1802 1315 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7066
NheI G'CTAG_C
Cuts at: 1052 2636 1052
Size: 1584 5482
NotI GC'GGCC_GC
Cuts at: 4513 4513
Size: 7066
NruI TCG'CGA
Cuts at: 4099 4099
Size: 7066
NspI r_CATG'y
Cuts at: 1132 1229 2146 4066 4424 5969 1132
Size: 97 917 1920 358 1545 2229
Fragments arranged by size:
2229 1920 1545 917 358 97
PasI CC'CwG_GG
Cuts at: 1348 3318 3466 1348
Size: 1970 148 4948
Fragments arranged by size:
4948 1970 148
PciI A'CATG_T
Cuts at: 5965 5965
Size: 7066
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2802 1108
Size: 1694 5372
PfoI T'CCnGG_A
Cuts at: 3489 3903 3489
Size: 414 6652
PmeI GTTT'AAAC
Cuts at: 4370 4370
Size: 7066
PmlI CAC'GTG
Cuts at: 3740 3740
Size: 7066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5135 5167 6648 6680 4286
Size: 32 817 32 1481 32 4672
Fragments arranged by size:
4672 1481 817 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2545 2566 2597 2545
Size: 21 31 7014
Fragments arranged by size:
7014 31 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7066
PsiI TTA'TAA
Cuts at: 4636 4636
Size: 7066
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4157 4189 4343 4375 4157
Size: 32 154 32 6848
Fragments arranged by size:
6848 154 32 32
PstI C_TGCA'G
Cuts at: 830 2094 2833 3189 3461 3562 3686 4172 4246
Size: 1264 739 356 272 101 124 486 74
Cuts at: 4246 4418 830
Size: 172 3478
Fragments arranged by size:
3478 1264 739 486 356 272 172 124
101 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7066
PvuII CAG'CTG
Cuts at: 2651 3076 3681 2651
Size: 425 605 6036
Fragments arranged by size:
6036 605 425
SacI G_AGCT'C
Cuts at: 3141 3284 3141
Size: 143 6923
SalI G'TCGA_C
Cuts at: 1936 4408 1936
Size: 2472 4594
SbfI CC_TGCA'GG
Cuts at: 4418 4418
Size: 7066
ScaI AGT'ACT
Cuts at: 4166 5314 7026 4166
Size: 1148 1712 4206
Fragments arranged by size:
4206 1712 1148
SfcI C'TryA_G
Cuts at: 826 1045 2090 2829 3185 3367 3457 3558 3682
Size: 219 1045 739 356 182 90 101 124
Cuts at: 3682 4168 4242 4414 5549 6230 6421 826
Size: 486 74 172 1135 681 191 1471
Fragments arranged by size:
1471 1135 1045 739 681 486 356 219
191 182 172 124 101 90 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 2073 2767 2073
Size: 694 6372
SfoI GGC'GCC
Cuts at: 2174 2375 3011 2174
Size: 201 636 6229
Fragments arranged by size:
6229 636 201
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7066
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7066
SmaI CCC'GGG
Cuts at: 1872 4393 1872
Size: 2521 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4346 5170 6071 6333 820
Size: 197 926 2403 824 901 262 1553
Fragments arranged by size:
2403 1553 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 4363 4857 493
Size: 3870 494 2702
Fragments arranged by size:
3870 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7066
SphI G_CATG'C
Cuts at: 1132 2146 4066 4424 1132
Size: 1014 1920 358 3774
Fragments arranged by size:
3774 1920 1014 358
SspI AAT'ATT
Cuts at: 5 52 4990 5
Size: 47 4938 2081
Fragments arranged by size:
4938 2081 47
StuI AGG'CCT
Cuts at: 1517 2228 3718 4206 1517
Size: 711 1490 488 4377
Fragments arranged by size:
4377 1490 711 488
StyI C'CwwG_G
Cuts at: 513 1065 2223 2548 2748 2772 2796 2867 4182
Size: 552 1158 325 200 24 24 71 1315
Cuts at: 4182 4505 513
Size: 323 3074
Fragments arranged by size:
3074 1315 1158 552 325 323 200 71
24 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2761 2966 3519 3537 3604 5093 5110
Size: 1255 1181 205 553 18 67 1489 17
Cuts at: 5110 5263 5448 5893 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1489 1255 1181 553 445 205 185
153 67 18 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3165 4164 5312
Size: 274 80 33 51 1443 1188 999 1148
Cuts at: 5312 7024 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1188 1148 999 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7026 7058 7026
Size: 32 7034
Tth111I GACn'n_nGTC
Cuts at: 1316 3911 1316
Size: 2595 4471
XbaI T'CTAG_A
Cuts at: 4402 4402
Size: 7066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3952 3952
Size: 7066
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7066
XmaI C'CCGG_G
Cuts at: 1870 4391 1870
Size: 2521 4545
XmnI GAAnn'nnTTC
Cuts at: 5195 5195
Size: 7066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4874 276
Size: 53 83 186 936 3340 2468
Fragments arranged by size:
3340 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NsiI PacI PspOMI PspXI RsrII SacII SanDI
SapI SexAI SrfI SwaI