Restriction map for
pFN21ASDA0932
(Circular) (Six-base) MAPSORT of: pFN21ASDA0932.seq Check: 8641 from: 1 to: 7774
pFN21ASDA0932 7774 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3958 4281 4949 3958
Size: 323 668 6783
Fragments arranged by size:
6783 668 323
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4038 5584 278
Size: 53 83 186 936 2502 1546 2468
Fragments arranged by size:
2502 2468 1546 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5117 1937
Size: 3180 4594
Acc65I G'GTAC_C
Cuts at: 3132 5095 3132
Size: 1963 5811
AclI AA'CG_TT
Cuts at: 1563 5901 6274 1563
Size: 4338 373 3063
Fragments arranged by size:
4338 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3986 4180 4721 4728 5837 7221 7489 1632
Size: 2354 194 541 7 1109 1384 268 1917
Fragments arranged by size:
2354 1917 1384 1109 541 268 194 7
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7577
AflIII A'CryG_T
Cuts at: 1203 2806 6673 7650 1203
Size: 1603 3867 977 1327
Fragments arranged by size:
3867 1603 1327 977
AgeI A'CCGG_T
Cuts at: 6605 6605
Size: 7774
AhdI GACnn_n'nnGTC
Cuts at: 6503 6503
Size: 7774
AleI CACnn'nnGTG
Cuts at: 1166 2595 2597 3636 1166
Size: 1429 2 1039 5304
Fragments arranged by size:
5304 1429 1039 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4166 1184
Size: 2982 4792
AlwNI CAG_nnn'CTG
Cuts at: 2167 2268 2655 4104 5517 7089 2167
Size: 101 387 1449 1413 1572 2852
Fragments arranged by size:
2852 1572 1449 1413 387 101
ApaI G_GGCC'C
Cuts at: 2050 4204 2050
Size: 2154 5620
ApaLI G'TGCA_C
Cuts at: 4350 4673 5831 6987 4350
Size: 323 1158 1156 5137
Fragments arranged by size:
5137 1158 1156 323
ApoI r'AATT_y
Cuts at: 1493 5083 5309 5461 7570 1493
Size: 3590 226 152 2109 1697
Fragments arranged by size:
3590 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6328 160
Size: 872 5296 1606
Fragments arranged by size:
5296 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2099 2457 4762 5099 1870
Size: 73 85 71 358 2305 337 4545
Fragments arranged by size:
4545 2305 358 337 85 73 71
BamHI G'GATC_C
Cuts at: 5104 5471 5104
Size: 367 7407
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2366 2679 3132 5095
Size: 325 217 627 300 279 313 453 1963
Cuts at: 5095 6550 618
Size: 1455 1842
Fragments arranged by size:
1963 1842 1455 627 453 325 313 300
279 217
BanII G_rGCy'C
Cuts at: 1391 2050 2885 3321 3674 4204 4391 4728 5093
Size: 659 835 436 353 530 187 337 365
Cuts at: 5093 1391
Size: 4072
Fragments arranged by size:
4072 835 659 530 436 365 353 337
187
BbeI G_GCGC'C
Cuts at: 2091 2683 2091
Size: 592 7182
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6803
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3554 3588 5965 5999 1903
Size: 34 1617 34 2377 34 3678
Fragments arranged by size:
3678 2377 1617 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3951 5666 6876 3951
Size: 1715 1210 4849
Fragments arranged by size:
4849 1715 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7774
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6385 136
Size: 107 122 71 631 605 4713 1525
Fragments arranged by size:
4713 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7769 7769
Size: 7774
BlpI GC'TnA_GC
Cuts at: 4900 5191 4900
Size: 291 7483
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2156 3622 4204 4354 4677 5835 6991
Size: 260 106 1466 582 150 323 1158 1156
Cuts at: 6991 1790
Size: 2573
Fragments arranged by size:
2573 1466 1158 1156 582 323 260 150
106
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7774
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2550 2590 4735
Size: 416 53 83 399 24 1133 40 2145
Cuts at: 4735 4792 5505 6463 442
Size: 57 713 958 1753
Fragments arranged by size:
2145 1753 1133 958 713 416 399 83
57 53 40 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4484
Size: 7774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2523 3178 3289 3517 6434 1239
Size: 950 334 655 111 228 2917 2579
Fragments arranged by size:
2917 2579 950 655 334 228 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2464 2926 3027 5899 6764 7062 2464
Size: 462 101 2872 865 298 3176
Fragments arranged by size:
3176 2872 865 462 298 101
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2793 3074 6437 882
Size: 617 1294 281 3363 2219
Fragments arranged by size:
3363 2219 1294 617 281
BsaAI yAC'GTr
Cuts at: 493 1204 3247 3526 5071 5565 493
Size: 711 2043 279 1545 494 2702
Fragments arranged by size:
2702 2043 1545 711 494 279
BsaBI GATnn'nnATC
Cuts at: 7768 7768
Size: 7774
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2115 2680 2991
Size: 53 83 186 936 555 27 565 311
Cuts at: 2991 4035 4083 4118 5581 5963 6598 275
Size: 1044 48 35 1463 382 635 1451
Fragments arranged by size:
1463 1451 1044 936 635 565 555 382
311 186 83 53 48 35 27
BsaWI w'CCGG_w
Cuts at: 3463 3990 4827 6207 6605 6879 7026 7437 3463
Size: 527 837 1380 398 274 147 411 3800
Fragments arranged by size:
3800 1380 837 527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2437 2467 3980 4010 4151
Size: 30 165 30 1034 30 1513 30 141
Cuts at: 4151 4166 4181 4196 4411 4441 1178
Size: 15 15 15 215 30 4511
Fragments arranged by size:
4511 1513 1034 215 165 141 30 30
30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2520 2700 2703 2961 4859 4862 1186
Size: 976 358 180 3 258 1898 3 4098
Fragments arranged by size:
4098 1898 976 358 258 180 3 3
BseYI C'CCAG_C
Cuts at: 2752 2870 3067 3808 4412 6977 7473 2752
Size: 118 197 741 604 2565 496 3053
Fragments arranged by size:
3053 2565 741 604 496 197 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2022 2396 3096 4076 4309 4315 4670
Size: 573 286 374 700 980 233 6 355
Cuts at: 4670 4733 5039 1163
Size: 63 306 3898
Fragments arranged by size:
3898 980 700 573 374 355 306 286
233 63 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4918 5224 5985 7013 1540
Size: 275 186 2917 306 761 1028 2301
Fragments arranged by size:
2917 2301 1028 761 306 275 186
BsiHKAI G_wGCw'C
Cuts at: 2885 3674 4354 4677 4728 5835 5920 6991 2885
Size: 789 680 323 51 1107 85 1071 3668
Fragments arranged by size:
3668 1107 1071 789 680 323 85 51
BsiWI C'GTAC_G
Cuts at: 3399 3399
Size: 7774
BsmI GAATG_Cn'
Cuts at: 5285 5378 5285
Size: 93 7681
BsmBI CGTCTCn'nnnn_
Cuts at: 3331 4109 4186 3331
Size: 778 77 6919
Fragments arranged by size:
6919 778 77
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2156 2885 3321 3622 3674 4204
Size: 399 260 106 729 436 301 52 530
Cuts at: 4204 4354 4391 4677 4728 5093 5835 5920 6991
Size: 150 37 286 51 365 742 85 1071
Cuts at: 6991 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 729 530 436 399 365
301 286 260 150 106 85 52 51
37
BspEI T'CCGG_A
Cuts at: 3463 4827 7437 3463
Size: 1364 2610 3800
Fragments arranged by size:
3800 2610 1364
BspHI T'CATG_A
Cuts at: 5663 7393 5663
Size: 1730 6044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3282 3419 3958 4089 4281 4949 5129 844
Size: 2438 137 539 131 192 668 180 3489
Fragments arranged by size:
3489 2438 668 539 192 180 137 131
BsrBI CCG'CTC
Cuts at: 1394 1823 3983 5661 1394
Size: 429 2160 1678 3507
Fragments arranged by size:
3507 2160 1678 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2214 4036 6269 6443 65
Size: 1183 966 1822 2233 174 1396
Fragments arranged by size:
2233 1822 1396 1183 966 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 3437 6418 6605 1700
Size: 110 1529 98 2981 187 2869
Fragments arranged by size:
2981 2869 1529 187 110 98
BsrGI T'GTAC_A
Cuts at: 96 3057 96
Size: 2961 4813
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7774
BssSI C'ACGA_G
Cuts at: 2747 4316 5834 6846 2747
Size: 1569 1518 1012 3675
Fragments arranged by size:
3675 1569 1518 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4104 4686 794
Size: 3310 582 3882
Fragments arranged by size:
3882 3310 582
BstBI TT'CG_AA
Cuts at: 2007 6578 2007
Size: 4571 3203
BstEII G'GTnAC_C
Cuts at: 1184 3169 1184
Size: 1985 5789
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2765 3638 4322 2356
Size: 409 873 684 5808
Fragments arranged by size:
5808 873 684 409
BstYI r'GATC_y
Cuts at: 1972 3162 3309 3993 5104 5471 5856 5873 7325
Size: 1190 147 684 1111 367 385 17 1452
Cuts at: 7325 7411 7423 7769 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1190 1111 684 385 367 346
147 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2355 3207 3330 3606 4596 513
Size: 552 1290 852 123 276 990 3691
Fragments arranged by size:
3691 1290 990 852 552 276 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3776 487
Size: 683 56 2550 4485
Fragments arranged by size:
4485 2550 683 56
BtsI GCAGTG_nn'
Cuts at: 2057 3148 4372 4677 5290 6084 6104 2057
Size: 1091 1224 305 613 794 20 3727
Fragments arranged by size:
3727 1224 1091 794 613 305 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7774
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2168 2203 529
Size: 35 1604 35 6100
Fragments arranged by size:
6100 1604 35 35
DraI TTT'AAA
Cuts at: 3220 5078 3220
Size: 1858 5916
DraIII CAC_nnn'GTG
Cuts at: 4462 4686 4462
Size: 224 7550
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2807 2990 4178 6781 809
Size: 507 1491 183 1188 2603 1802
Fragments arranged by size:
2603 1802 1491 1188 507 183
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2389 2789 3007
Size: 54 1425 50 275 18 559 400 218
Cuts at: 3007 3628 3767 3849 3929 4915 5221 6110 7739
Size: 621 139 82 80 986 306 889 1629
Cuts at: 7739 8
Size: 43
Fragments arranged by size:
1629 1425 986 889 621 559 400 306
275 218 139 82 80 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4915 5221 1537
Size: 275 3103 306 4090
Fragments arranged by size:
4090 3103 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 2461 3606 4447 5704 1353
Size: 960 148 1145 841 1257 3423
Fragments arranged by size:
3423 1257 1145 960 841 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6361 6735 6881 387
Size: 1064 138 4772 374 146 1280
Fragments arranged by size:
4772 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2883 3672 4726 2883
Size: 789 1054 5931
Fragments arranged by size:
5931 1054 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2523 3178 3289 3517 3986 4180
Size: 393 557 334 655 111 228 469 194
Cuts at: 4180 4721 4728 5837 6434 7221 7489 1239
Size: 541 7 1109 597 787 268 1524
Fragments arranged by size:
1524 1109 787 655 597 557 541 469
393 334 268 228 194 111 7
EcoO109I rG'GnC_Cy
Cuts at: 2046 2047 2424 4200 4377 2046
Size: 1 377 1776 177 5443
Fragments arranged by size:
5443 1776 377 177 1
EcoRI G'AATT_C
Cuts at: 5083 5461 5083
Size: 378 7396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3299 3406 4066 4210 3299
Size: 107 660 144 6863
Fragments arranged by size:
6863 660 144 107
FspI TGC'GCA
Cuts at: 4064 6280 4064
Size: 2216 5558
HaeII r_GCGC'y
Cuts at: 1678 2091 2110 2683 4907 6921 1678
Size: 413 19 573 2224 2014 2531
Fragments arranged by size:
2531 2224 2014 573 413 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 2437 2469 2496
Size: 32 135 32 740 32 93 32 27
Cuts at: 2496 2528 3436 3468 3650 3682 4151 4183 6415
Size: 32 908 32 182 32 469 32 2232
Cuts at: 6415 6447 6489 6521 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2232 908 740 469 182 135 93
42 32 32 32 32 32 32 32
32 32 32 27
HincII GTy'rAC
Cuts at: 669 1657 1938 4267 5118 5364 669
Size: 988 281 2329 851 246 3079
Fragments arranged by size:
3079 2329 988 851 281 246
HindIII A'AGCT_T
Cuts at: 748 2629 3543 748
Size: 1881 914 4979
Fragments arranged by size:
4979 1881 914
HpaI GTT'AAC
Cuts at: 5364 5364
Size: 7774
KasI G'GCGC_C
Cuts at: 2087 2679 2087
Size: 592 7182
KpnI G_GTAC'C
Cuts at: 3136 5099 3136
Size: 1963 5811
MfeI C'AATT_G
Cuts at: 5373 5373
Size: 7774
MluI A'CGCG_T
Cuts at: 7650 7650
Size: 7774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3699 4194 6888 7072 1305
Size: 2394 495 2694 184 2007
Fragments arranged by size:
2694 2394 2007 495 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3009 3769 10
Size: 54 1425 343 1177 760 4015
Fragments arranged by size:
4015 1425 1177 760 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2595 2597 3206 3636 3795 4154
Size: 648 1188 241 2 609 430 159 359
Cuts at: 4154 4320 5732 6091 6250 518
Size: 166 1412 359 159 2042
Fragments arranged by size:
2042 1412 1188 648 609 430 359 359
241 166 159 159 2
MspA1I CmG'CkG
Cuts at: 1606 2077 2167 2198 3570 4068 4776 5190 5867
Size: 471 90 31 1372 498 708 414 677
Cuts at: 5867 7015 7260 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1372 1148 708 677 498 471 414
245 90 31
NaeI GCC'GGC
Cuts at: 3341 3341
Size: 7774
NarI GG'CG_CC
Cuts at: 2088 2680 2088
Size: 592 7182
NcoI C'CATG_G
Cuts at: 513 1065 2355 3207 513
Size: 552 1290 852 5080
Fragments arranged by size:
5080 1290 852 552
NdeI CA'TA_TG
Cuts at: 387 3577 387
Size: 3190 4584
NgoMIV G'CCGG_C
Cuts at: 3339 3339
Size: 7774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7774
NotI GC'GGCC_GC
Cuts at: 5221 5221
Size: 7774
NspI r_CATG'y
Cuts at: 1132 1229 2435 2810 4153 5132 6677 1132
Size: 97 1206 375 1343 979 1545 2229
Fragments arranged by size:
2229 1545 1343 1206 979 375 97
PasI CC'CwG_GG
Cuts at: 1348 2420 3176 3923 1348
Size: 1072 756 747 5199
Fragments arranged by size:
5199 1072 756 747
PciI A'CATG_T
Cuts at: 2806 6673 2806
Size: 3867 3907
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2268 3005 3074 1108
Size: 1160 737 69 5808
Fragments arranged by size:
5808 1160 737 69
PfoI T'CCnGG_A
Cuts at: 3255 3312 3594 5043 3255
Size: 57 282 1449 5986
Fragments arranged by size:
5986 1449 282 57
PmeI GTTT'AAAC
Cuts at: 5078 5078
Size: 7774
PmlI CAC'GTG
Cuts at: 3526 3526
Size: 7774
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 5843 5875 7356 7388 2924
Size: 32 2887 32 1481 32 3310
Fragments arranged by size:
3310 2887 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2424 4377 2424
Size: 1953 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7774
PsiI TTA'TAA
Cuts at: 5344 5344
Size: 7774
PspOMI G'GGCC_C
Cuts at: 2046 4200 2046
Size: 2154 5620
PstI C_TGCA'G
Cuts at: 830 3090 4453 4960 5126 830
Size: 2260 1363 507 166 3478
Fragments arranged by size:
3478 2260 1363 507 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7774
PvuII CAG'CTG
Cuts at: 2167 3570 2167
Size: 1403 6371
SacI G_AGCT'C
Cuts at: 2885 3674 4728 2885
Size: 789 1054 5931
Fragments arranged by size:
5931 1054 789
SalI G'TCGA_C
Cuts at: 1936 5116 1936
Size: 3180 4594
SanDI GG'GwC_CC
Cuts at: 4377 4377
Size: 7774
SapI GCTCTTCn'nnn_
Cuts at: 2313 2461 2313
Size: 148 7626
SbfI CC_TGCA'GG
Cuts at: 3090 5126 3090
Size: 2036 5738
ScaI AGT'ACT
Cuts at: 6022 7734 6022
Size: 1712 6062
SexAI A'CCwGG_T
Cuts at: 3115 3115
Size: 7774
SfcI C'TryA_G
Cuts at: 826 1045 3086 3472 4449 4956 5122 6257 6938
Size: 219 2041 386 977 507 166 1135 681
Cuts at: 6938 7129 826
Size: 191 1471
Fragments arranged by size:
2041 1471 1135 977 681 507 386 219
191 166
SfoI GGC'GCC
Cuts at: 2089 2681 2089
Size: 592 7182
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7774
SmaI CCC'GGG
Cuts at: 1872 2030 2459 5101 1872
Size: 158 429 2642 4545
Fragments arranged by size:
4545 2642 429 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2479 2941 3042 5878 6779 7041
Size: 197 926 536 462 101 2836 901 262
Cuts at: 7041 820
Size: 1553
Fragments arranged by size:
2836 1553 926 901 536 462 262 197
101
SnaBI TAC'GTA
Cuts at: 493 5071 5565 493
Size: 4578 494 2702
Fragments arranged by size:
4578 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7774
SphI G_CATG'C
Cuts at: 1132 2435 5132 1132
Size: 1303 2697 3774
Fragments arranged by size:
3774 2697 1303
SspI AAT'ATT
Cuts at: 5 52 5698 5
Size: 47 5646 2081
Fragments arranged by size:
5646 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7774
StyI C'CwwG_G
Cuts at: 513 1065 2355 2370 3207 3483 4233 5213 513
Size: 552 1290 15 837 276 750 980 3074
Fragments arranged by size:
3074 1290 980 837 750 552 276 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3157 3337 3915 4774 5801 5818 5971
Size: 1255 1577 180 578 859 1027 17 153
Cuts at: 5971 6156 6601 325
Size: 185 445 1498
Fragments arranged by size:
1577 1498 1255 1027 859 578 445 185
180 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3057 6020 7732
Size: 274 80 33 51 1443 1080 2963 1712
Cuts at: 7732 96
Size: 138
Fragments arranged by size:
2963 1712 1443 1080 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2707 2739 2953 2985 3105 3137 7734 7766 2707
Size: 32 214 32 120 32 4597 32 2715
Fragments arranged by size:
4597 2715 214 120 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7774
XbaI T'CTAG_A
Cuts at: 5110 5110
Size: 7774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 4742 2281
Size: 2461 5313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7774
XmaI C'CCGG_G
Cuts at: 1870 2028 2457 5099 1870
Size: 158 429 2642 4545
Fragments arranged by size:
4545 2642 429 158
XmnI GAAnn'nnTTC
Cuts at: 2936 3106 4661 5903 2936
Size: 170 1555 1242 4807
Fragments arranged by size:
4807 1555 1242 170
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4036 5582 276
Size: 53 83 186 936 2502 1546 2468
Fragments arranged by size:
2502 2468 1546 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacII SfiI SrfI SwaI