Restriction map for
pFN21ASDA0927
(Circular) (Six-base) MAPSORT of: pFN21ASDA0927.seq Check: 7642 from: 1 to: 7576
pFN21ASDA0927 7576 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2383 3507 3809 4388 2383
Size: 1124 302 579 5571
Fragments arranged by size:
5571 1124 579 302
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4649 5386 278
Size: 53 83 186 936 3113 737 2468
Fragments arranged by size:
3113 2468 936 737 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2964 3129 4919 1937
Size: 1027 165 1790 4594
Fragments arranged by size:
4594 1790 1027 165
Acc65I G'GTAC_C
Cuts at: 4897 4897
Size: 7576
AclI AA'CG_TT
Cuts at: 1563 5703 6076 1563
Size: 4140 373 3063
Fragments arranged by size:
4140 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 5639 7023 7291 1632
Size: 597 3410 1384 268 1917
Fragments arranged by size:
3410 1917 1384 597 268
AfeI AGC'GCT
Cuts at: 2106 2106
Size: 7576
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7379
AflIII A'CryG_T
Cuts at: 1203 6475 7452 1203
Size: 5272 977 1327
Fragments arranged by size:
5272 1327 977
AgeI A'CCGG_T
Cuts at: 6407 6407
Size: 7576
AhdI GACnn_n'nnGTC
Cuts at: 6305 6305
Size: 7576
AleI CACnn'nnGTG
Cuts at: 1166 3255 1166
Size: 2089 5487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3872 1184
Size: 2688 4888
AlwNI CAG_nnn'CTG
Cuts at: 2444 3493 4039 4138 5319 6891 2444
Size: 1049 546 99 1181 1572 3129
Fragments arranged by size:
3129 1572 1181 1049 546 99
ApaI G_GGCC'C
Cuts at: 3833 4608 3833
Size: 775 6801
ApaLI G'TGCA_C
Cuts at: 2944 3389 5633 6789 2944
Size: 445 2244 1156 3731
Fragments arranged by size:
3731 2244 1156 445
ApoI r'AATT_y
Cuts at: 1493 4885 5111 5263 7372 1493
Size: 3392 226 152 2109 1697
Fragments arranged by size:
3392 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6130 160
Size: 872 5098 1606
Fragments arranged by size:
5098 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 2684 3669 3956 4268 4901 1870
Size: 73 183 558 985 287 312 633 4545
Fragments arranged by size:
4545 985 633 558 312 287 183 73
BamHI G'GATC_C
Cuts at: 4906 5273 4906
Size: 367 7209
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4897 6352 618
Size: 325 217 627 3110 1455 1842
Fragments arranged by size:
3110 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2176 2988 3297 3360 3732 3833 3888
Size: 705 80 812 309 63 372 101 55
Cuts at: 3888 3918 3963 4551 4608 4895 1391
Size: 30 45 588 57 287 4072
Fragments arranged by size:
4072 812 705 588 372 309 287 101
80 63 57 55 45 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6605
BbvCI CC'TCA_GC
Cuts at: 2417 4394 2417
Size: 1977 5599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2117 2151 4629 4663 5767 5801 1903
Size: 34 180 34 2478 34 1104 34 3678
Fragments arranged by size:
3678 2478 1104 180 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4469 5468 6678 4469
Size: 999 1210 5367
Fragments arranged by size:
5367 1210 999
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7576
BfrBI ATG'CAT
Cuts at: 3321 3849 3321
Size: 528 7048
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2575 2714 3285
Size: 107 122 71 631 605 903 139 571
Cuts at: 3285 3674 6187 136
Size: 389 2513 1525
Fragments arranged by size:
2513 1525 903 631 605 571 389 139
122 107 71
BglII A'GATC_T
Cuts at: 2485 7571 2485
Size: 5086 2490
BlpI GC'TnA_GC
Cuts at: 3194 3367 3623 4993 3194
Size: 173 256 1370 5777
Fragments arranged by size:
5777 1370 256 173
Bme1580I G_kGCm'C
Cuts at: 1790 2571 2948 3393 3833 4608 5637 6793 1790
Size: 781 377 445 440 775 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 781 775 445 440 377
BmgBI CAC'GTC
Cuts at: 1318 3657 1318
Size: 2339 5237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2229 2784 5307
Size: 416 53 83 399 24 812 555 2523
Cuts at: 5307 6265 442
Size: 958 1753
Fragments arranged by size:
2523 1753 958 812 555 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2142 1056
Size: 1086 6490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4535
Size: 7576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2887 2960 2972 3281 3364 3462 3842
Size: 890 758 73 12 309 83 98 380
Cuts at: 3842 4420 6236 1239
Size: 578 1816 2579
Fragments arranged by size:
2579 1816 890 758 578 380 309 98
83 73 12
Bpu10I CC'TnA_GC
Cuts at: 2417 4394 2417
Size: 1977 5599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 5701 6566 6864 2372
Size: 3329 865 298 3084
Fragments arranged by size:
3329 3084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2795 6239 882
Size: 617 1296 3444 2219
Fragments arranged by size:
3444 2219 1296 617
BsaAI yAC'GTr
Cuts at: 493 1204 3967 4000 4873 5367 493
Size: 711 2763 33 873 494 2702
Fragments arranged by size:
2763 2702 873 711 494 33
BsaBI GATnn'nnATC
Cuts at: 7570 7570
Size: 7576
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4646 5383 5765 6400
Size: 53 83 186 936 3113 737 382 635
Cuts at: 6400 275
Size: 1451
Fragments arranged by size:
3113 1451 936 737 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3135 6009 6407 6681 6828 7239 3135
Size: 2874 398 274 147 411 3472
Fragments arranged by size:
3472 2874 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2885 2915 3107 3137 3372
Size: 30 165 30 1482 30 192 30 235
Cuts at: 3372 3402 1178
Size: 30 5352
Fragments arranged by size:
5352 1482 235 192 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2480 2485 2488 2567 2804 4135 4448
Size: 1165 129 5 3 79 237 1331 313
Cuts at: 4448 4731 1186
Size: 283 4031
Fragments arranged by size:
4031 1331 1165 313 283 237 129 79
5 3
BseYI C'CCAG_C
Cuts at: 2260 2311 2463 3929 4711 6779 7275 2260
Size: 51 152 1466 782 2068 496 2561
Fragments arranged by size:
2561 2068 1466 782 496 152 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2280 3233 3479 3485 3913 1163
Size: 573 544 953 246 6 428 4826
Fragments arranged by size:
4826 953 573 544 428 246 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2039 3409 5026 5787 6815 1540
Size: 275 186 38 1370 1617 761 1028 2301
Fragments arranged by size:
2301 1617 1370 1028 761 275 186 38
BsiHKAI G_wGCw'C
Cuts at: 2205 2948 2988 3297 3393 3918 4551 5637 5722
Size: 743 40 309 96 525 633 1086 85
Cuts at: 5722 6793 2205
Size: 1071 2988
Fragments arranged by size:
2988 1086 1071 743 633 525 309 96
85 40
BsmI GAATG_Cn'
Cuts at: 3851 5087 5180 3851
Size: 1236 93 6247
Fragments arranged by size:
6247 1236 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4637 4637
Size: 7576
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2176 2205 2571 2948 2988 3297
Size: 399 306 80 29 366 377 40 309
Cuts at: 3297 3360 3393 3732 3833 3888 3918 3963 4551
Size: 63 33 339 101 55 30 45 588
Cuts at: 4551 4608 4895 5637 5722 6793 1391
Size: 57 287 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 399 377 366 339
309 306 287 101 85 80 63 57
55 45 40 33 30 29
BspEI T'CCGG_A
Cuts at: 3135 7239 3135
Size: 4104 3472
BspHI T'CATG_A
Cuts at: 4221 5465 7195 4221
Size: 1244 1730 4602
Fragments arranged by size:
4602 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2383 3085 3255 3507 3809 4388 4512 4931
Size: 1539 702 170 252 302 579 124 419
Cuts at: 4931 844
Size: 3489
Fragments arranged by size:
3489 1539 702 579 419 302 252 170
124
BsrBI CCG'CTC
Cuts at: 1394 1823 5463 1394
Size: 429 3640 3507
Fragments arranged by size:
3640 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6071 6245 65
Size: 1183 4823 174 1396
Fragments arranged by size:
4823 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3213 6220 6407 1700
Size: 110 1403 3007 187 2869
Fragments arranged by size:
3007 2869 1403 187 110
BsrGI T'GTAC_A
Cuts at: 96 4488 96
Size: 4392 3184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7576
BssSI C'ACGA_G
Cuts at: 5636 6648 5636
Size: 1012 6564
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2294 2359 794
Size: 1500 65 6011
Fragments arranged by size:
6011 1500 65
BstBI TT'CG_AA
Cuts at: 2007 6380 2007
Size: 4373 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7576
BstXI CCAn_nnnn'nTGG
Cuts at: 2740 4596 2740
Size: 1856 5720
BstYI r'GATC_y
Cuts at: 1972 2061 2485 3993 4017 4272 4906 5273 5658
Size: 89 424 1508 24 255 634 367 385
Cuts at: 5658 5675 7127 7213 7225 7571 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1508 1452 634 424 385 367 346
255 89 86 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 2167 2886 2167
Size: 719 6857
BtgI C'CryG_G
Cuts at: 513 1065 2028 2046 2056 2502 2739 2829 3034
Size: 552 963 18 10 446 237 90 205
Cuts at: 3034 4803 513
Size: 1769 3286
Fragments arranged by size:
3286 1769 963 552 446 237 205 90
18 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6837
Fragments arranged by size:
6837 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 2915 3274 3393 3718 5092 5886 5906 2872
Size: 43 359 119 325 1374 794 20 4542
Fragments arranged by size:
4542 1374 794 359 325 119 43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7541
DraI TTT'AAA
Cuts at: 4880 4880
Size: 7576
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3565 6583 809
Size: 507 2249 3018 1802
Fragments arranged by size:
3018 2249 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 3066 3406
Size: 54 1425 50 275 18 206 1030 340
Cuts at: 3406 3811 3834 5023 5912 7541 8
Size: 405 23 1189 889 1629 43
Fragments arranged by size:
1629 1425 1189 1030 889 405 340 275
206 54 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2036 3406 5023 1537
Size: 275 224 1370 1617 4090
Fragments arranged by size:
4090 1617 1370 275 224
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2205 2990 3579 4002 5506 1353
Size: 774 78 785 589 423 1504 3423
Fragments arranged by size:
3423 1504 785 774 589 423 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 4751 6163 6537 6683 387
Size: 1064 138 678 2484 1412 374 146 1280
Fragments arranged by size:
2484 1412 1280 1064 678 374 146 138
EcoICRI GAG'CTC
Cuts at: 2986 3295 3916 4549 2986
Size: 309 621 633 6013
Fragments arranged by size:
6013 633 621 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2229 2887 2960 2972 3281 3364
Size: 393 497 100 658 73 12 309 83
Cuts at: 3364 3462 3842 4420 5639 6236 7023 7291 1239
Size: 98 380 578 1219 597 787 268 1524
Fragments arranged by size:
1524 1219 787 658 597 578 497 393
380 309 268 100 98 83 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3086 3378 3824 3086
Size: 292 446 6838
Fragments arranged by size:
6838 446 292
EcoO109I rG'GnC_Cy
Cuts at: 2150 2460 2479 2527 2716 3162 3616 3829 3862
Size: 310 19 48 189 446 454 213 33
Cuts at: 3862 4390 4605 2150
Size: 528 215 5121
Fragments arranged by size:
5121 528 454 446 310 215 213 189
48 33 19
EcoRI G'AATT_C
Cuts at: 4885 5263 4885
Size: 378 7198
EcoRV GAT'ATC
Cuts at: 4030 4030
Size: 7576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2588 2374
Size: 214 7362
FspI TGC'GCA
Cuts at: 6082 6082
Size: 7576
HaeII r_GCGC'y
Cuts at: 1678 2108 2231 3306 6723 1678
Size: 430 123 1075 3417 2531
Fragments arranged by size:
3417 2531 1075 430 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2173 2205 2477 2509 2543
Size: 32 135 32 601 32 272 32 34
Cuts at: 2543 2575 3236 3268 3460 3492 3641 3673 4241
Size: 32 661 32 192 32 149 32 568
Cuts at: 4241 4273 4311 4343 4467 4499 6217 6249 6291
Size: 32 38 32 124 32 1718 32 42
Cuts at: 6291 6323 1373
Size: 32 2626
Fragments arranged by size:
2626 1718 661 601 568 272 192 149
135 124 42 38 34 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4920 5166 669
Size: 988 281 2982 246 3079
Fragments arranged by size:
3079 2982 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7576
HpaI GTT'AAC
Cuts at: 5166 5166
Size: 7576
KpnI G_GTAC'C
Cuts at: 4901 4901
Size: 7576
MfeI C'AATT_G
Cuts at: 5175 5175
Size: 7576
MluI A'CGCG_T
Cuts at: 7452 7452
Size: 7576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3684 3786 4779 6690 6874 1305
Size: 2379 102 993 1911 184 2007
Fragments arranged by size:
2379 2007 1911 993 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3068 10
Size: 54 1425 343 1236 4518
Fragments arranged by size:
4518 1425 1236 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3239 3255 3723 3854 4526 5534 5893
Size: 648 2073 16 468 131 672 1008 359
Cuts at: 5893 6052 518
Size: 159 2042
Fragments arranged by size:
2073 2042 1008 672 648 468 359 159
131 16
MspA1I CmG'CkG
Cuts at: 1606 2030 2048 2058 2326 2416 3036 3552 4398
Size: 424 18 10 268 90 620 516 846
Cuts at: 4398 4572 4992 5669 6817 7062 1606
Size: 174 420 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 677 620 516 424 420
268 245 174 90 18 10
NaeI GCC'GGC
Cuts at: 3215 3215
Size: 7576
NcoI C'CATG_G
Cuts at: 513 1065 2739 513
Size: 552 1674 5350
Fragments arranged by size:
5350 1674 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7576
NgoMIV G'CCGG_C
Cuts at: 3213 3213
Size: 7576
NheI G'CTAG_C
Cuts at: 1052 2138 1052
Size: 1086 6490
NotI GC'GGCC_GC
Cuts at: 5023 5023
Size: 7576
NsiI A_TGCA'T
Cuts at: 3323 3851 3323
Size: 528 7048
NspI r_CATG'y
Cuts at: 1132 1229 3321 4449 4934 6479 1132
Size: 97 2092 1128 485 1545 2229
Fragments arranged by size:
2229 2092 1545 1128 485 97
PasI CC'CwG_GG
Cuts at: 1348 2476 4457 1348
Size: 1128 1981 4467
Fragments arranged by size:
4467 1981 1128
PciI A'CATG_T
Cuts at: 6475 6475
Size: 7576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3820 4102 1108
Size: 2712 282 4582
Fragments arranged by size:
4582 2712 282
PfoI T'CCnGG_A
Cuts at: 3144 3144
Size: 7576
PmeI GTTT'AAAC
Cuts at: 4880 4880
Size: 7576
PmlI CAC'GTG
Cuts at: 4000 4000
Size: 7576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5645 5677 7158 7190 5645
Size: 32 1481 32 6031
Fragments arranged by size:
6031 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2150 2460 2527 3162 3862 4390 2150
Size: 310 67 635 700 528 5336
Fragments arranged by size:
5336 700 635 528 310 67
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7576
PsiI TTA'TAA
Cuts at: 5146 5146
Size: 7576
PspOMI G'GGCC_C
Cuts at: 3829 4604 3829
Size: 775 6801
PstI C_TGCA'G
Cuts at: 830 2867 4928 830
Size: 2037 2061 3478
Fragments arranged by size:
3478 2061 2037
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7576
PvuII CAG'CTG
Cuts at: 2416 4398 2416
Size: 1982 5594
SacI G_AGCT'C
Cuts at: 2988 3297 3918 4551 2988
Size: 309 621 633 6013
Fragments arranged by size:
6013 633 621 309
SacII CC_GC'GG
Cuts at: 2031 2049 2059 3037 2031
Size: 18 10 978 6570
Fragments arranged by size:
6570 978 18 10
SalI G'TCGA_C
Cuts at: 1936 4918 1936
Size: 2982 4594
SanDI GG'GwC_CC
Cuts at: 2460 2527 3862 2460
Size: 67 1335 6174
Fragments arranged by size:
6174 1335 67
SapI GCTCTTCn'nnn_
Cuts at: 2127 3579 2127
Size: 1452 6124
SbfI CC_TGCA'GG
Cuts at: 4928 4928
Size: 7576
ScaI AGT'ACT
Cuts at: 5824 7536 5824
Size: 1712 5864
SfcI C'TryA_G
Cuts at: 826 1045 2863 3786 4789 4924 6059 6740 6931
Size: 219 1818 923 1003 135 1135 681 191
Cuts at: 6931 826
Size: 1471
Fragments arranged by size:
1818 1471 1135 1003 923 681 219 191
135
SfiI GGCCn_nnn'nGGCC
Cuts at: 2714 3674 2714
Size: 960 6616
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7576
SmaI CCC'GGG
Cuts at: 1872 2686 3671 4903 1872
Size: 814 985 1232 4545
Fragments arranged by size:
4545 1232 985 814
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 5680 6581 6843 820
Size: 197 926 444 3293 901 262 1553
Fragments arranged by size:
3293 1553 926 901 444 262 197
SnaBI TAC'GTA
Cuts at: 493 4873 5367 493
Size: 4380 494 2702
Fragments arranged by size:
4380 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7576
SphI G_CATG'C
Cuts at: 1132 4934 1132
Size: 3802 3774
SrfI GCCC'GGGC
Cuts at: 3671 3671
Size: 7576
SspI AAT'ATT
Cuts at: 5 52 5500 5
Size: 47 5448 2081
Fragments arranged by size:
5448 2081 47
StuI AGG'CCT
Cuts at: 1517 3185 1517
Size: 1668 5908
StyI C'CwwG_G
Cuts at: 513 1065 2424 2739 3157 4114 5015 513
Size: 552 1359 315 418 957 901 3074
Fragments arranged by size:
3074 1359 957 901 552 418 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4544 5603 5620 5773 5958 6403 325
Size: 1255 2964 1059 17 153 185 445 1498
Fragments arranged by size:
2964 1498 1255 1059 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4140 4488 4776
Size: 274 80 33 51 1443 2163 348 288
Cuts at: 4776 5822 7534 96
Size: 1046 1712 138
Fragments arranged by size:
2163 1712 1443 1046 348 288 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3372 3404 7536 7568 3372
Size: 32 4132 32 3380
Fragments arranged by size:
4132 3380 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2776 4085 1316
Size: 1460 1309 4807
Fragments arranged by size:
4807 1460 1309
XbaI T'CTAG_A
Cuts at: 4912 4912
Size: 7576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2746 3037 2746
Size: 291 7285
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7576
XmaI C'CCGG_G
Cuts at: 1870 2684 3669 4901 1870
Size: 814 985 1232 4545
Fragments arranged by size:
4545 1232 985 814
XmnI GAAnn'nnTTC
Cuts at: 5705 5705
Size: 7576
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4647 5384 276
Size: 53 83 186 936 3113 737 2468
Fragments arranged by size:
3113 2468 936 737 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII
FseI FspAI KasI NarI NruI PacI PspXI PsrI
RsrII SexAI SfoI SwaI