Restriction map for
pFN21ASDA0920
(Circular) (Six-base) MAPSORT of: pFN21ASDA0920.seq Check: 9023 from: 1 to: 8038
pFN21ASDA0920 8038 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3148 4459 3148
Size: 1311 6727
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5848 278
Size: 53 83 186 936 4312 2468
Fragments arranged by size:
4312 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5381 1937
Size: 3444 4594
Acc65I G'GTAC_C
Cuts at: 5359 5359
Size: 8038
AclI AA'CG_TT
Cuts at: 1563 6165 6538 1563
Size: 4602 373 3063
Fragments arranged by size:
4602 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 2378 3333 4545 4566 4674 4943 5079
Size: 621 125 955 1212 21 108 269 136
Cuts at: 5079 5112 6101 7485 7753 1632
Size: 33 989 1384 268 1917
Fragments arranged by size:
1917 1384 1212 989 955 621 269 268
136 125 108 33 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7841
AflIII A'CryG_T
Cuts at: 1203 6937 7914 1203
Size: 5734 977 1327
Fragments arranged by size:
5734 1327 977
AgeI A'CCGG_T
Cuts at: 6869 6869
Size: 8038
AhdI GACnn_n'nnGTC
Cuts at: 4147 6767 4147
Size: 2620 5418
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8038
AlwNI CAG_nnn'CTG
Cuts at: 2281 2957 3550 3611 4693 5781 7353 2281
Size: 676 593 61 1082 1088 1572 2966
Fragments arranged by size:
2966 1572 1088 1082 676 593 61
ApaI G_GGCC'C
Cuts at: 3966 3993 4683 3966
Size: 27 690 7321
Fragments arranged by size:
7321 690 27
ApaLI G'TGCA_C
Cuts at: 6095 7251 6095
Size: 1156 6882
ApoI r'AATT_y
Cuts at: 1493 3718 4022 4261 5347 5573 5725 7834 1493
Size: 2225 304 239 1086 226 152 2109 1697
Fragments arranged by size:
2225 2109 1697 1086 304 239 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6592 160
Size: 872 5560 1606
Fragments arranged by size:
5560 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8038
AvaI C'yCGr_G
Cuts at: 1870 1943 3204 4035 5240 5363 1870
Size: 73 1261 831 1205 123 4545
Fragments arranged by size:
4545 1261 1205 831 123 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4158 4191 4158
Size: 33 8005
BamHI G'GATC_C
Cuts at: 5368 5735 5368
Size: 367 7671
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 3160 3866 3899 5359
Size: 325 217 627 1259 114 706 33 1460
Cuts at: 5359 6814 618
Size: 1455 1842
Fragments arranged by size:
1842 1460 1455 1259 706 627 325 217
114 33
BanII G_rGCy'C
Cuts at: 1391 3066 3390 3966 3993 4683 4961 5357 1391
Size: 1675 324 576 27 690 278 396 4072
Fragments arranged by size:
4072 1675 690 576 396 324 278 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3864 4596 4662 5151 5328 928
Size: 971 1965 732 66 489 177 3638
Fragments arranged by size:
3638 1965 971 732 489 177 66
BbvCI CC'TCA_GC
Cuts at: 3871 4011 4847 3871
Size: 140 836 7062
Fragments arranged by size:
7062 836 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6229 6263 1903
Size: 34 4292 34 3678
Fragments arranged by size:
4292 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4159 5930 7140 4159
Size: 1771 1210 5057
Fragments arranged by size:
5057 1771 1210
BclI T'GATC_A
Cuts at: 1548 2833 4351 1548
Size: 1285 1518 5235
Fragments arranged by size:
5235 1518 1285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6649 136
Size: 107 122 71 631 605 4977 1525
Fragments arranged by size:
4977 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4094 8033 4094
Size: 3939 4099
BlpI GC'TnA_GC
Cuts at: 4779 5455 4779
Size: 676 7362
Bme1580I G_kGCm'C
Cuts at: 1790 3535 3904 3966 3993 4683 6099 7255 1790
Size: 1745 369 62 27 690 1416 1156 2573
Fragments arranged by size:
2573 1745 1416 1156 690 369 62 27
BmgBI CAC'GTC
Cuts at: 1318 4825 1318
Size: 3507 4531
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4704 5769 6727
Size: 416 53 83 399 24 3287 1065 958
Cuts at: 6727 442
Size: 1753
Fragments arranged by size:
3287 1753 1065 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3039 1056
Size: 1983 6055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 4147 2578
Size: 1569 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2386 2538 2696 3383 3512 3837 3926
Size: 1064 83 152 158 687 129 325 89
Cuts at: 3926 4199 4238 4975 4998 6698 1239
Size: 273 39 737 23 1700 2579
Fragments arranged by size:
2579 1700 1064 737 687 325 273 158
152 129 89 83 39 23
Bpu10I CC'TnA_GC
Cuts at: 2984 3871 4011 4667 4847 4902 2984
Size: 887 140 656 180 55 6120
Fragments arranged by size:
6120 887 656 180 140 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4797 6163 7028 7326 4075
Size: 722 1366 865 298 4787
Fragments arranged by size:
4787 1366 865 722 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3823 3902 4164 4175 4727 6701 882
Size: 617 2324 79 262 11 552 1974 2219
Fragments arranged by size:
2324 2219 1974 617 552 262 79 11
BsaAI yAC'GTr
Cuts at: 493 1204 5335 5829 493
Size: 711 4131 494 2702
Fragments arranged by size:
4131 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 8032 8032
Size: 8038
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4332 5845 6227 6862
Size: 53 83 186 936 2799 1513 382 635
Cuts at: 6862 275
Size: 1451
Fragments arranged by size:
2799 1513 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6471 6869 7143 7290 7701 6471
Size: 398 274 147 411 6808
Fragments arranged by size:
6808 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2420 2436 2450 2777
Size: 30 165 30 1003 14 16 14 327
Cuts at: 2777 2807 3233 3263 4317 4347 1178
Size: 30 426 30 1054 30 4869
Fragments arranged by size:
4869 1054 1003 426 327 165 30 30
30 30 30 16 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 3042 3368 3394 3482 4136 4338 4745
Size: 1036 820 326 26 88 654 202 407
Cuts at: 4745 4931 5031 1186
Size: 186 100 4193
Fragments arranged by size:
4193 1036 820 654 407 326 202 186
100 88 26
BseYI C'CCAG_C
Cuts at: 2439 2613 3009 3122 3142 3173 4381 4686 5066
Size: 174 396 113 20 31 1208 305 380
Cuts at: 5066 5109 5286 7241 7737 2439
Size: 43 177 1955 496 2740
Fragments arranged by size:
2740 1955 1208 496 396 380 305 177
174 113 43 31 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2982 3586 4457 4724 1163
Size: 573 1246 604 871 267 4477
Fragments arranged by size:
4477 1246 871 604 573 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5488 6249 7277 1540
Size: 275 186 3487 761 1028 2301
Fragments arranged by size:
3487 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3066 3390 6099 6184 7255 3066
Size: 324 2709 85 1071 3849
Fragments arranged by size:
3849 2709 1071 324 85
BsmI GAATG_Cn'
Cuts at: 2140 4447 4978 5549 5642 2140
Size: 2307 531 571 93 4536
Fragments arranged by size:
4536 2307 571 531 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4339 4339
Size: 8038
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3066 3390 3535 3904 3966 3993 4683
Size: 399 1276 324 145 369 62 27 690
Cuts at: 4683 4961 5357 6099 6184 7255 1391
Size: 278 396 742 85 1071 2174
Fragments arranged by size:
2174 1276 1071 742 690 399 396 369
324 278 145 85 62 27
BspEI T'CCGG_A
Cuts at: 7701 7701
Size: 8038
BspHI T'CATG_A
Cuts at: 3622 5927 7657 3622
Size: 2305 1730 4003
Fragments arranged by size:
4003 2305 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2460 3148 4459 5393 844
Size: 1616 688 1311 934 3489
Fragments arranged by size:
3489 1616 1311 934 688
BsrBI CCG'CTC
Cuts at: 1394 1823 2513 5925 1394
Size: 429 690 3412 3507
Fragments arranged by size:
3507 3412 690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3423 6533 6707 65
Size: 1183 2175 3110 174 1396
Fragments arranged by size:
3110 2175 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3039 6682 6869 1700
Size: 110 1229 3643 187 2869
Fragments arranged by size:
3643 2869 1229 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8038
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8038
BssSI C'ACGA_G
Cuts at: 2999 6098 7110 2999
Size: 3099 1012 3927
Fragments arranged by size:
3927 3099 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8038
BstBI TT'CG_AA
Cuts at: 2007 6842 2007
Size: 4835 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8038
BstXI CCAn_nnnn'nTGG
Cuts at: 2674 4177 2674
Size: 1503 6535
BstYI r'GATC_y
Cuts at: 1972 2871 4094 4890 5298 5368 5735 6120 6137
Size: 899 1223 796 408 70 367 385 17
Cuts at: 6137 7589 7675 7687 8033 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1223 899 796 408 385 367
346 86 70 17 12
Bsu36I CC'TnA_GG
Cuts at: 4596 4596
Size: 8038
BtgI C'CryG_G
Cuts at: 513 1065 2022 2781 3087 4069 513
Size: 552 957 759 306 982 4482
Fragments arranged by size:
4482 982 957 759 552 306
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3576 487
Size: 683 56 2350 4949
Fragments arranged by size:
4949 2350 683 56
BtsI GCAGTG_nn'
Cuts at: 2970 4691 4982 5554 6348 6368 2970
Size: 1721 291 572 794 20 4640
Fragments arranged by size:
4640 1721 794 572 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8003
DraI TTT'AAA
Cuts at: 5342 5342
Size: 8038
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7045 809
Size: 507 5729 1802
Fragments arranged by size:
5729 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3112 3735 4806
Size: 54 1425 50 275 18 1282 623 1071
Cuts at: 4806 5485 6374 8003 8
Size: 679 889 1629 43
Fragments arranged by size:
1629 1425 1282 1071 889 679 623 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5485 1537
Size: 275 3673 4090
Fragments arranged by size:
4090 3673 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2229 2307 3622 4881 4908 4919 5088
Size: 846 30 78 1315 1259 27 11 169
Cuts at: 5088 5968 1353
Size: 880 3423
Fragments arranged by size:
3423 1315 1259 880 846 169 78 30
27 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2048 6625 6999 7145 387
Size: 1064 138 459 4577 374 146 1280
Fragments arranged by size:
4577 1280 1064 459 374 146 138
EcoICRI GAG'CTC
Cuts at: 3064 3388 3064
Size: 324 7714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2303 2378 2386 2538 2696 3333
Size: 393 621 50 75 8 152 158 637
Cuts at: 3333 3383 3512 3837 3926 4199 4238 4545 4566
Size: 50 129 325 89 273 39 307 21
Cuts at: 4566 4674 4943 4975 4998 5079 5112 6101 6698
Size: 108 269 32 23 81 33 989 597
Cuts at: 6698 7485 7753 1239
Size: 787 268 1524
Fragments arranged by size:
1524 989 787 637 621 597 393 325
307 273 269 268 158 152 129 108
89 81 75 50 50 39 33 32
23 21 8
EcoO109I rG'GnC_Cy
Cuts at: 3848 3962 3963 3990 4520 4679 4680 5063 5236
Size: 114 1 27 530 159 1 383 173
Cuts at: 5236 3848
Size: 6650
Fragments arranged by size:
6650 530 383 173 159 114 27 1
1
EcoRI G'AATT_C
Cuts at: 3718 5347 5725 3718
Size: 1629 378 6031
Fragments arranged by size:
6031 1629 378
EcoRV GAT'ATC
Cuts at: 2325 2632 2325
Size: 307 7731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4142 4142
Size: 8038
FspI TGC'GCA
Cuts at: 6544 6544
Size: 8038
HaeII r_GCGC'y
Cuts at: 1678 4035 4667 7185 1678
Size: 2357 632 2518 2531
Fragments arranged by size:
2531 2518 2357 632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 2863 2895 3375
Size: 32 135 32 1205 32 54 32 480
Cuts at: 3375 3407 3899 3931 4066 4073 4098 4105 4133
Size: 32 492 32 135 7 25 7 28
Cuts at: 4133 4165 4172 4204 4295 4317 4327 4349 6679
Size: 32 7 32 91 22 10 22 2330
Cuts at: 6679 6711 6753 6785 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2330 1205 492 480 135 135 91
54 42 32 32 32 32 32 32
32 32 32 32 28 25 22 22
10 7 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3802 5382 5628 669
Size: 988 281 1864 1580 246 3079
Fragments arranged by size:
3079 1864 1580 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8038
HpaI GTT'AAC
Cuts at: 5628 5628
Size: 8038
KpnI G_GTAC'C
Cuts at: 5363 5363
Size: 8038
MfeI C'AATT_G
Cuts at: 5637 5637
Size: 8038
MluI A'CGCG_T
Cuts at: 7914 7914
Size: 8038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7152 7336 1305
Size: 5847 184 2007
Fragments arranged by size:
5847 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3114 3737 4808 10
Size: 54 1425 343 1282 623 1071 3240
Fragments arranged by size:
3240 1425 1282 1071 623 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5996 6355 6514 518
Size: 648 4830 359 159 2042
Fragments arranged by size:
4830 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2192 3475 3709 4224 4700 4778 4864 5113
Size: 586 1283 234 515 476 78 86 249
Cuts at: 5113 5454 6131 7279 7524 1606
Size: 341 677 1148 245 2120
Fragments arranged by size:
2120 1283 1148 677 586 515 476 341
249 245 234 86 78
NaeI GCC'GGC
Cuts at: 3041 3041
Size: 8038
NcoI C'CATG_G
Cuts at: 513 1065 2022 2781 3087 4069 513
Size: 552 957 759 306 982 4482
Fragments arranged by size:
4482 982 957 759 552 306
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8038
NgoMIV G'CCGG_C
Cuts at: 3039 3039
Size: 8038
NheI G'CTAG_C
Cuts at: 1052 3035 1052
Size: 1983 6055
NotI GC'GGCC_GC
Cuts at: 5485 5485
Size: 8038
NspI r_CATG'y
Cuts at: 1132 1229 4585 5001 5396 6941 1132
Size: 97 3356 416 395 1545 2229
Fragments arranged by size:
3356 2229 1545 416 395 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8038
PciI A'CATG_T
Cuts at: 6937 6937
Size: 8038
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2489 2957 1108
Size: 1381 468 6189
Fragments arranged by size:
6189 1381 468
PfoI T'CCnGG_A
Cuts at: 2673 2755 4176 4254 4853 2673
Size: 82 1421 78 599 5858
Fragments arranged by size:
5858 1421 599 82 78
PmeI GTTT'AAAC
Cuts at: 5342 5342
Size: 8038
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 2260 6107 6139 7620 7652 2228
Size: 32 3847 32 1481 32 2614
Fragments arranged by size:
3847 2614 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3848 4520 5236 3848
Size: 672 716 6650
Fragments arranged by size:
6650 716 672
PshAI GACnn'nnGTC
Cuts at: 1613 4134 1613
Size: 2521 5517
PsiI TTA'TAA
Cuts at: 5608 5608
Size: 8038
PspOMI G'GGCC_C
Cuts at: 3962 3989 4679 3962
Size: 27 690 7321
Fragments arranged by size:
7321 690 27
PspXI vC'TCGA_Gb
Cuts at: 5240 5240
Size: 8038
PstI C_TGCA'G
Cuts at: 830 2139 2181 3159 3558 5076 5390 830
Size: 1309 42 978 399 1518 314 3478
Fragments arranged by size:
3478 1518 1309 978 399 314 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8038
PvuII CAG'CTG
Cuts at: 3475 3709 4700 4778 3475
Size: 234 991 78 6735
Fragments arranged by size:
6735 991 234 78
SacI G_AGCT'C
Cuts at: 3066 3390 3066
Size: 324 7714
SalI G'TCGA_C
Cuts at: 1936 5380 1936
Size: 3444 4594
SbfI CC_TGCA'GG
Cuts at: 3159 5390 3159
Size: 2231 5807
ScaI AGT'ACT
Cuts at: 4766 6286 7998 4766
Size: 1520 1712 4806
Fragments arranged by size:
4806 1712 1520
SfcI C'TryA_G
Cuts at: 826 1045 2135 2177 2659 3155 3554 3781 3967
Size: 219 1090 42 482 496 399 227 186
Cuts at: 3967 4533 5072 5386 6521 7202 7393 826
Size: 566 539 314 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 681 566 539 496 482
399 314 227 219 191 186 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8038
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8038
SmaI CCC'GGG
Cuts at: 1872 4037 5365 1872
Size: 2165 1328 4545
Fragments arranged by size:
4545 2165 1328
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 4812 5240 6142 7043 7305
Size: 197 926 2147 722 428 902 901 262
Cuts at: 7305 820
Size: 1553
Fragments arranged by size:
2147 1553 926 902 901 722 428 262
197
SnaBI TAC'GTA
Cuts at: 493 5335 5829 493
Size: 4842 494 2702
Fragments arranged by size:
4842 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8038
SphI G_CATG'C
Cuts at: 1132 5396 1132
Size: 4264 3774
SrfI GCCC'GGGC
Cuts at: 4037 4037
Size: 8038
SspI AAT'ATT
Cuts at: 5 52 5962 5
Size: 47 5910 2081
Fragments arranged by size:
5910 2081 47
StuI AGG'CCT
Cuts at: 1517 3346 3787 1517
Size: 1829 441 5768
Fragments arranged by size:
5768 1829 441
StyI C'CwwG_G
Cuts at: 513 1065 2022 2781 3087 3738 3879 4069 4410
Size: 552 957 759 306 651 141 190 341
Cuts at: 4410 5477 513
Size: 1067 3074
Fragments arranged by size:
3074 1067 957 759 651 552 341 306
190 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3769 6065 6082 6235 6420 6865 325
Size: 1255 2189 2296 17 153 185 445 1498
Fragments arranged by size:
2296 2189 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3507 3749 4764
Size: 274 80 33 51 1443 1530 242 1015
Cuts at: 4764 6284 7996 96
Size: 1520 1712 138
Fragments arranged by size:
1712 1530 1520 1443 1015 274 242 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2828 2860 7998 8030 2828
Size: 32 5138 32 2836
Fragments arranged by size:
5138 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3662 4870 1316
Size: 2346 1208 4484
Fragments arranged by size:
4484 2346 1208
XbaI T'CTAG_A
Cuts at: 2881 5374 2881
Size: 2493 5545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3730 3730
Size: 8038
XhoI C'TCGA_G
Cuts at: 1943 5240 1943
Size: 3297 4741
XmaI C'CCGG_G
Cuts at: 1870 4035 5363 1870
Size: 2165 1328 4545
Fragments arranged by size:
4545 2165 1328
XmnI GAAnn'nnTTC
Cuts at: 2396 6167 2396
Size: 3771 4267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5846 276
Size: 53 83 186 936 4312 2468
Fragments arranged by size:
4312 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
DraIII EcoNI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SwaI