Restriction map for
pFN21ASDA0915
(Circular) (Six-base) MAPSORT of: pFN21ASDA0915.seq Check: 3447 from: 1 to: 7252
pFN21ASDA0915 7252 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4336 4386 4336
Size: 50 7202
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3305 5062 278
Size: 53 83 186 936 1769 1757 2468
Fragments arranged by size:
2468 1769 1757 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4595 1937
Size: 2658 4594
Acc65I G'GTAC_C
Cuts at: 4573 4573
Size: 7252
AclI AA'CG_TT
Cuts at: 1563 5379 5752 1563
Size: 3816 373 3063
Fragments arranged by size:
3816 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2526 2958 3056 3867 4359 4413 4424 4503
Size: 894 432 98 811 492 54 11 79
Cuts at: 4503 5315 6699 6967 1632
Size: 812 1384 268 1917
Fragments arranged by size:
1917 1384 894 812 811 492 432 268
98 79 54 11
AfeI AGC'GCT
Cuts at: 3820 4223 3820
Size: 403 6849
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7055
AflIII A'CryG_T
Cuts at: 1203 6151 7128 1203
Size: 4948 977 1327
Fragments arranged by size:
4948 1327 977
AgeI A'CCGG_T
Cuts at: 6083 6083
Size: 7252
AhdI GACnn_n'nnGTC
Cuts at: 5981 5981
Size: 7252
AleI CACnn'nnGTG
Cuts at: 1166 3350 3605 1166
Size: 2184 255 4813
Fragments arranged by size:
4813 2184 255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2429 2861 3737 1184
Size: 1245 432 876 4699
Fragments arranged by size:
4699 1245 876 432
AlwNI CAG_nnn'CTG
Cuts at: 2357 2462 2627 2999 4075 4306 4995 6567 2357
Size: 105 165 372 1076 231 689 1572 3042
Fragments arranged by size:
3042 1572 1076 689 372 231 165 105
ApaLI G'TGCA_C
Cuts at: 2194 5309 6465 2194
Size: 3115 1156 2981
Fragments arranged by size:
3115 2981 1156
ApoI r'AATT_y
Cuts at: 1493 3929 4561 4787 4939 7048 1493
Size: 2436 632 226 152 2109 1697
Fragments arranged by size:
2436 2109 1697 632 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5806 160
Size: 872 4774 1606
Fragments arranged by size:
4774 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7252
AvaI C'yCGr_G
Cuts at: 1870 1943 4499 4577 1870
Size: 73 2556 78 4545
Fragments arranged by size:
4545 2556 78 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3062 3095 3062
Size: 33 7219
BamHI G'GATC_C
Cuts at: 4120 4582 4949 4120
Size: 462 367 6423
Fragments arranged by size:
6423 462 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 3921 3957 4473 4523
Size: 325 217 627 911 1223 36 516 50
Cuts at: 4523 4573 6028 618
Size: 50 1455 1842
Fragments arranged by size:
1842 1455 1223 911 627 516 325 217
50 50 36
BanII G_rGCy'C
Cuts at: 1391 2217 2518 2691 4571 1391
Size: 826 301 173 1880 4072
Fragments arranged by size:
4072 1880 826 301 173
BbeI G_GCGC'C
Cuts at: 3925 3925
Size: 7252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3233 928
Size: 971 1334 4947
Fragments arranged by size:
4947 1334 971
BbvCI CC'TCA_GC
Cuts at: 2257 2455 3254 3822 2257
Size: 198 799 568 5687
Fragments arranged by size:
5687 799 568 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 5443 5477 1903
Size: 34 1850 34 1622 34 3678
Fragments arranged by size:
3678 1850 1622 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3241 5144 6354 3241
Size: 1903 1210 4139
Fragments arranged by size:
4139 1903 1210
BclI T'GATC_A
Cuts at: 1548 3391 1548
Size: 1843 5409
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3779 5863 136
Size: 107 122 71 631 605 2107 2084 1525
Fragments arranged by size:
2107 2084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2920 7247 2920
Size: 4327 2925
BlpI GC'TnA_GC
Cuts at: 2832 3356 4669 2832
Size: 524 1313 5415
Fragments arranged by size:
5415 1313 524
Bme1580I G_kGCm'C
Cuts at: 1790 2198 5313 6469 1790
Size: 408 3115 1156 2573
Fragments arranged by size:
3115 2573 1156 408
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7252
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2026 2110 4983
Size: 416 53 83 399 24 609 84 2873
Cuts at: 4983 5941 442
Size: 958 1753
Fragments arranged by size:
2873 1753 958 609 416 399 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2598 3162 2291
Size: 307 564 6381
Fragments arranged by size:
6381 564 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 2448 2604 2705 2976 3132 3180 4373
Size: 1116 93 156 101 271 156 48 1193
Cuts at: 4373 5912 1239
Size: 1539 2579
Fragments arranged by size:
2579 1539 1193 1116 271 156 156 101
93 48
Bpu10I CC'TnA_GC
Cuts at: 2257 2455 2517 3254 3822 2257
Size: 198 62 737 568 5687
Fragments arranged by size:
5687 737 568 198 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2840 5377 6242 6540 2216
Size: 624 2537 865 298 2928
Fragments arranged by size:
2928 2537 865 624 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2373 2549 3572 3913 5915 882
Size: 617 874 176 1023 341 2002 2219
Fragments arranged by size:
2219 2002 1023 874 617 341 176
BsaAI yAC'GTr
Cuts at: 493 1204 4549 5043 493
Size: 711 3345 494 2702
Fragments arranged by size:
3345 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2925 7246 2925
Size: 4321 2931
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3302 3922 5059 5441
Size: 53 83 186 936 1769 620 1137 382
Cuts at: 5441 6076 275
Size: 635 1451
Fragments arranged by size:
1769 1451 1137 936 635 620 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4117 5685 6083 6357 6504 6915 4117
Size: 1568 398 274 147 411 4454
Fragments arranged by size:
4454 1568 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 3308 3338 1178
Size: 30 165 30 723 30 1152 30 5092
Fragments arranged by size:
5092 1152 723 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 2732 2843 2967 2979 3032 3125 3590
Size: 948 598 111 124 12 53 93 465
Cuts at: 3590 4483 1186
Size: 893 3955
Fragments arranged by size:
3955 948 893 598 465 124 111 93
53 12
BseYI C'CCAG_C
Cuts at: 2218 4107 4228 4431 6455 6951 2218
Size: 1889 121 203 2024 496 2519
Fragments arranged by size:
2519 2024 1889 496 203 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 3437 4308 4364 1163
Size: 573 653 1048 871 56 4051
Fragments arranged by size:
4051 1048 871 653 573 56
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4702 5463 6491 1540
Size: 275 186 2701 761 1028 2301
Fragments arranged by size:
2701 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 2620 2691 3978 5313 5398 6469 2198
Size: 422 71 1287 1335 85 1071 2981
Fragments arranged by size:
2981 1335 1287 1071 422 85 71
BsmI GAATG_Cn'
Cuts at: 3775 4763 4856 3775
Size: 988 93 6171
Fragments arranged by size:
6171 988 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2217 2518 2620 2691 3978 4571
Size: 399 408 19 301 102 71 1287 593
Cuts at: 4571 5313 5398 6469 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1287 1071 742 593 408 399 301
102 85 71 19
BspEI T'CCGG_A
Cuts at: 4117 6915 4117
Size: 2798 4454
BspHI T'CATG_A
Cuts at: 5141 6871 5141
Size: 1730 5522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2365 2478 3327 4038 4102 4336 4386 4607
Size: 1521 113 849 711 64 234 50 221
Cuts at: 4607 844
Size: 3489
Fragments arranged by size:
3489 1521 849 711 234 221 113 64
50
BsrBI CCG'CTC
Cuts at: 1394 1823 2823 3442 3658 5139 1394
Size: 429 1000 619 216 1481 3507
Fragments arranged by size:
3507 1481 1000 619 429 216
BsrDI GCAATG_nn'
Cuts at: 65 1248 5747 5921 65
Size: 1183 4499 174 1396
Fragments arranged by size:
4499 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3630 5896 6083 1700
Size: 110 1820 2266 187 2869
Fragments arranged by size:
2869 2266 1820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7252
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7252
BssSI C'ACGA_G
Cuts at: 2874 5312 6324 2874
Size: 2438 1012 3802
Fragments arranged by size:
3802 2438 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7252
BstBI TT'CG_AA
Cuts at: 2007 6056 2007
Size: 4049 3203
BstEII G'GTnAC_C
Cuts at: 1184 2397 2792 1184
Size: 1213 395 5644
Fragments arranged by size:
5644 1213 395
BstYI r'GATC_y
Cuts at: 1972 2920 4120 4582 4949 5334 5351 6803 6889
Size: 948 1200 462 367 385 17 1452 86
Cuts at: 6889 6901 7247 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1200 948 462 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2421 513
Size: 552 1356 5344
Fragments arranged by size:
5344 1356 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4073 487
Size: 683 56 2847 3666
Fragments arranged by size:
3666 2847 683 56
BtsI GCAGTG_nn'
Cuts at: 2826 4768 5562 5582 2826
Size: 1942 794 20 4496
Fragments arranged by size:
4496 1942 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7252
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7217
DraI TTT'AAA
Cuts at: 4556 4556
Size: 7252
DraIII CAC_nnn'GTG
Cuts at: 3207 3523 3207
Size: 316 6936
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6259 809
Size: 507 4943 1802
Fragments arranged by size:
4943 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4699 5588 7217
Size: 54 1425 50 275 18 2869 889 1629
Cuts at: 7217 8
Size: 43
Fragments arranged by size:
2869 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4699 1537
Size: 275 2887 4090
Fragments arranged by size:
4090 2887 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3009 3410 3481 3741 3843 5182 1353
Size: 1656 401 71 260 102 1339 3423
Fragments arranged by size:
3423 1656 1339 401 260 102 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 2332 3607 4133 5839 6213
Size: 1064 138 483 260 1275 526 1706 374
Cuts at: 6213 6359 387
Size: 146 1280
Fragments arranged by size:
1706 1280 1275 1064 526 483 374 260
146 138
EcoICRI GAG'CTC
Cuts at: 2689 2689
Size: 7252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2448 2526 2604 2705 2958 2976
Size: 393 723 93 78 78 101 253 18
Cuts at: 2976 3056 3132 3180 3867 4359 4373 4413 4424
Size: 80 76 48 687 492 14 40 11
Cuts at: 4424 4503 5315 5912 6699 6967 1239
Size: 79 812 597 787 268 1524
Fragments arranged by size:
1524 812 787 723 687 597 492 393
268 253 101 93 80 79 78 78
76 48 40 18 14 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3209 4100 4518 3209
Size: 891 418 5943
Fragments arranged by size:
5943 891 418
EcoO109I rG'GnC_Cy
Cuts at: 2126 2601 2722 2731 2905 3527 3781 3895 4250
Size: 475 121 9 174 622 254 114 355
Cuts at: 4250 4419 2126
Size: 169 4959
Fragments arranged by size:
4959 622 475 355 254 174 169 121
114 9
EcoRI G'AATT_C
Cuts at: 3929 4561 4939 3929
Size: 632 378 6242
Fragments arranged by size:
6242 632 378
FspI TGC'GCA
Cuts at: 3713 5758 3713
Size: 2045 5207
HaeII r_GCGC'y
Cuts at: 1678 2341 3822 3925 4225 6399 1678
Size: 663 1481 103 300 2174 2531
Fragments arranged by size:
2531 2174 1481 663 300 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5893 5925 5967 5999 1373
Size: 32 135 32 4321 32 42 32 2626
Fragments arranged by size:
4321 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4596 4842 669
Size: 988 281 2658 246 3079
Fragments arranged by size:
3079 2658 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4197 748
Size: 3449 3803
HpaI GTT'AAC
Cuts at: 4842 4842
Size: 7252
KasI G'GCGC_C
Cuts at: 3921 3921
Size: 7252
KpnI G_GTAC'C
Cuts at: 4577 4577
Size: 7252
MfeI C'AATT_G
Cuts at: 4851 4851
Size: 7252
MluI A'CGCG_T
Cuts at: 7128 7128
Size: 7252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4489 6366 6550 1305
Size: 3184 1877 184 2007
Fragments arranged by size:
3184 2007 1877 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5430
Fragments arranged by size:
5430 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3350 3605 5210 5569 5728 518
Size: 648 2184 255 1605 359 159 2042
Fragments arranged by size:
2184 2042 1605 648 359 255 159
MspA1I CmG'CkG
Cuts at: 1606 2222 2752 3237 3667 4063 4435 4668 5345
Size: 616 530 485 430 396 372 233 677
Cuts at: 5345 6493 6738 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 616 530 485 430 396
372 245 233
NaeI GCC'GGC
Cuts at: 3632 3632
Size: 7252
NarI GG'CG_CC
Cuts at: 3922 3922
Size: 7252
NcoI C'CATG_G
Cuts at: 513 1065 2421 513
Size: 552 1356 5344
Fragments arranged by size:
5344 1356 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7252
NgoMIV G'CCGG_C
Cuts at: 3630 3630
Size: 7252
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7252
NotI GC'GGCC_GC
Cuts at: 4699 4699
Size: 7252
NspI r_CATG'y
Cuts at: 1132 1229 3277 3515 3718 4219 4610 6155 1132
Size: 97 2048 238 203 501 391 1545 2229
Fragments arranged by size:
2229 2048 1545 501 391 238 203 97
PasI CC'CwG_GG
Cuts at: 1348 2123 2727 3784 1348
Size: 775 604 1057 4816
Fragments arranged by size:
4816 1057 775 604
PciI A'CATG_T
Cuts at: 6151 6151
Size: 7252
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2208 2627 3523 1108
Size: 1100 419 896 4837
Fragments arranged by size:
4837 1100 896 419
PfoI T'CCnGG_A
Cuts at: 3369 3369
Size: 7252
PmeI GTTT'AAAC
Cuts at: 4556 4556
Size: 7252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5321 5353 6834 6866 5321
Size: 32 1481 32 5707
Fragments arranged by size:
5707 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2601 2722 2731 2905 2601
Size: 121 9 174 6948
Fragments arranged by size:
6948 174 121 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7252
PsiI TTA'TAA
Cuts at: 4822 4822
Size: 7252
PstI C_TGCA'G
Cuts at: 830 2047 3066 3639 3726 4604 830
Size: 1217 1019 573 87 878 3478
Fragments arranged by size:
3478 1217 1019 878 573 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7252
PvuII CAG'CTG
Cuts at: 2222 4435 2222
Size: 2213 5039
SacI G_AGCT'C
Cuts at: 2691 2691
Size: 7252
SalI G'TCGA_C
Cuts at: 1936 4594 1936
Size: 2658 4594
SanDI GG'GwC_CC
Cuts at: 2601 2731 2905 2601
Size: 130 174 6948
Fragments arranged by size:
6948 174 130
SapI GCTCTTCn'nnn_
Cuts at: 3481 3843 3481
Size: 362 6890
SbfI CC_TGCA'GG
Cuts at: 3066 4604 3066
Size: 1538 5714
ScaI AGT'ACT
Cuts at: 5500 7212 5500
Size: 1712 5540
SfcI C'TryA_G
Cuts at: 826 1045 2043 3062 3583 3635 3722 4600 5735
Size: 219 998 1019 521 52 87 878 1135
Cuts at: 5735 6416 6607 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1019 998 878 681 521 219
191 87 52
SfoI GGC'GCC
Cuts at: 3923 3923
Size: 7252
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7252
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7252
SmaI CCC'GGG
Cuts at: 1872 4579 1872
Size: 2707 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 2855 4499 5356 6257 6519
Size: 197 926 288 624 1644 857 901 262
Cuts at: 6519 820
Size: 1553
Fragments arranged by size:
1644 1553 926 901 857 624 288 262
197
SnaBI TAC'GTA
Cuts at: 493 4549 5043 493
Size: 4056 494 2702
Fragments arranged by size:
4056 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7252
SphI G_CATG'C
Cuts at: 1132 3277 3515 3718 4610 1132
Size: 2145 238 203 892 3774
Fragments arranged by size:
3774 2145 892 238 203
SspI AAT'ATT
Cuts at: 5 52 5176 5
Size: 47 5124 2081
Fragments arranged by size:
5124 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7252
StyI C'CwwG_G
Cuts at: 513 1065 2421 4691 513
Size: 552 1356 2270 3074
Fragments arranged by size:
3074 2270 1356 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2398 2686 2780 3116 3184 3356 3776
Size: 1255 818 288 94 336 68 172 420
Cuts at: 3776 3881 4196 5279 5296 5449 5634 6079 325
Size: 105 315 1083 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1083 818 445 420 336 315
288 185 172 153 105 94 68 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5498 7210 96
Size: 274 80 33 51 1443 3521 1712 138
Fragments arranged by size:
3521 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 2308 2340 3159 3191 7212 7244 2126
Size: 32 150 32 819 32 4021 32 2134
Fragments arranged by size:
4021 2134 819 150 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4347 1316
Size: 3031 4221
XbaI T'CTAG_A
Cuts at: 2264 4588 2264
Size: 2324 4928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3241 3241
Size: 7252
XhoI C'TCGA_G
Cuts at: 1943 4499 1943
Size: 2556 4696
XmaI C'CCGG_G
Cuts at: 1870 4577 1870
Size: 2707 4545
XmnI GAAnn'nnTTC
Cuts at: 5381 5381
Size: 7252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3303 5060 276
Size: 53 83 186 936 1769 1757 2468
Fragments arranged by size:
2468 1769 1757 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI
SfiI SrfI SwaI