Restriction map for
pFN21ASDA0908
(Circular) (Six-base) MAPSORT of: pFN21ASDA0908.seq Check: 8909 from: 1 to: 6244
pFN21ASDA0908 6244 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4054 278
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2910 3587 1937
Size: 973 677 4594
Fragments arranged by size:
4594 973 677
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 2323 4371 4744 1563
Size: 760 2048 373 3063
Fragments arranged by size:
3063 2048 760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2533 4307 5691 5959 1632
Size: 516 385 1774 1384 268 1917
Fragments arranged by size:
1917 1774 1384 516 385 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 2481 2496 5143 6120 1203
Size: 1278 15 2647 977 1327
Fragments arranged by size:
2647 1327 1278 977 15
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 4973 4973
Size: 6244
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 3987 5559 3987
Size: 1572 4672
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 2189 3026 3113 3273 3553 3779 3931 6040
Size: 696 837 87 160 280 226 152 2109
Cuts at: 6040 1493
Size: 1697
Fragments arranged by size:
2109 1697 837 696 280 226 160 152
87
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 3569 1870
Size: 73 153 1473 4545
Fragments arranged by size:
4545 1473 153 73
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 3565 5020 618
Size: 325 217 627 315 1463 1455 1842
Fragments arranged by size:
1842 1463 1455 627 325 315 217
BanII G_rGCy'C
Cuts at: 1391 2046 3563 1391
Size: 655 1517 4072
Fragments arranged by size:
4072 1517 655
BbeI G_GCGC'C
Cuts at: 2106 2106
Size: 6244
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2096 2130 4435 4469 1903
Size: 34 159 34 2305 34 3678
Fragments arranged by size:
3678 2305 159 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 3499 1548
Size: 1951 4293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 4855 136
Size: 107 122 71 631 605 428 2755 1525
Fragments arranged by size:
2755 1525 631 605 428 122 107 71
BglII A'GATC_T
Cuts at: 3240 6239 3240
Size: 2999 3245
BlpI GC'TnA_GC
Cuts at: 3661 3661
Size: 6244
Bme1580I G_kGCm'C
Cuts at: 1790 3183 3486 4305 5461 1790
Size: 1393 303 819 1156 2573
Fragments arranged by size:
2573 1393 1156 819 303
BmgBI CAC'GTC
Cuts at: 1318 2497 1318
Size: 1179 5065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2384 3975 4933
Size: 416 53 83 399 24 967 1591 958
Cuts at: 4933 442
Size: 1753
Fragments arranged by size:
1753 1591 967 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4904 1239
Size: 3665 2579
Bpu10I CC'TnA_GC
Cuts at: 2797 3207 2797
Size: 410 5834
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4369 5234 5532 4369
Size: 865 298 5081
Fragments arranged by size:
5081 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2348 4907 882
Size: 617 849 2559 2219
Fragments arranged by size:
2559 2219 849 617
BsaAI yAC'GTr
Cuts at: 493 1204 3541 4035 493
Size: 711 2337 494 2702
Fragments arranged by size:
2702 2337 711 494
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 4051 4433 5068
Size: 53 83 186 936 570 1948 382 635
Cuts at: 5068 275
Size: 1451
Fragments arranged by size:
1948 1451 936 635 570 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4677 5075 5349 5496 5907 4677
Size: 398 274 147 411 5014
Fragments arranged by size:
5014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 1178
Size: 30 165 30 874 30 5115
Fragments arranged by size:
5115 874 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 2423 2598 2870 1186
Size: 855 382 175 272 4560
Fragments arranged by size:
4560 855 382 272 175
BseYI C'CCAG_C
Cuts at: 2059 5447 5943 2059
Size: 3388 496 2360
Fragments arranged by size:
3388 2360 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 2282 2297 3429 3438 1163
Size: 573 365 181 15 1132 9 3969
Fragments arranged by size:
3969 1132 573 365 181 15 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3694 4455 5483 1540
Size: 275 186 1693 761 1028 2301
Fragments arranged by size:
2301 1693 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2119 4305 4390 5461 2046
Size: 73 2186 85 1071 2829
Fragments arranged by size:
2829 2186 1071 85 73
BsmI GAATG_Cn'
Cuts at: 2607 3755 3848 2607
Size: 1148 93 5003
Fragments arranged by size:
5003 1148 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2053 2053
Size: 6244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2119 3183 3486 3563 4305 4390
Size: 399 256 73 1064 303 77 742 85
Cuts at: 4390 5461 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1064 742 399 303 256 85
77 73
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 2511 2536 4133 5863 2511
Size: 25 1597 1730 2892
Fragments arranged by size:
2892 1730 1597 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 3599 844
Size: 1343 1412 3489
Fragments arranged by size:
3489 1412 1343
BsrBI CCG'CTC
Cuts at: 1394 1823 4131 1394
Size: 429 2308 3507
Fragments arranged by size:
3507 2308 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3435 4739 4913 65
Size: 1183 2187 1304 174 1396
Fragments arranged by size:
2187 1396 1304 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2639 4888 5075 1700
Size: 110 829 2249 187 2869
Fragments arranged by size:
2869 2249 829 187 110
BsrGI T'GTAC_A
Cuts at: 96 2847 96
Size: 2751 3493
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3416 3461 794
Size: 2622 45 3577
Fragments arranged by size:
3577 2622 45
BstBI TT'CG_AA
Cuts at: 2007 5048 2007
Size: 3041 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 2517 3202 3240 3574 3941 4326 4343 5795
Size: 545 685 38 334 367 385 17 1452
Cuts at: 5795 5881 5893 6239 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 685 545 385 367 346 334
86 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 3317 3420 513
Size: 552 957 1295 103 3337
Fragments arranged by size:
3337 1295 957 552 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5505
Fragments arranged by size:
5505 683 56
BtsI GCAGTG_nn'
Cuts at: 3760 4554 4574 3760
Size: 794 20 5430
Fragments arranged by size:
5430 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 2307 3279 3548 2307
Size: 972 269 5003
Fragments arranged by size:
5003 972 269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5251 809
Size: 507 3935 1802
Fragments arranged by size:
3935 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2698 2961 3691
Size: 54 1425 50 275 18 868 263 730
Cuts at: 3691 4580 6209 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 868 730 275 263 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 4174 1353
Size: 870 1951 3423
Fragments arranged by size:
3423 1951 870
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2109 4831 5205 5351 387
Size: 1064 138 520 2722 374 146 1280
Fragments arranged by size:
2722 1280 1064 520 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2044
Size: 6244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2533 4307 4904 5691 5959 1239
Size: 393 516 385 1774 597 787 268 1524
Fragments arranged by size:
1774 1524 787 597 516 393 385 268
EcoRI G'AATT_C
Cuts at: 3026 3553 3931 3026
Size: 527 378 5339
Fragments arranged by size:
5339 527 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2659 3275 2659
Size: 616 5628
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 2106 2455 5391 1678
Size: 428 349 2936 2531
Fragments arranged by size:
2936 2531 428 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 2277 2309 4885
Size: 32 135 32 645 32 28 32 2576
Cuts at: 4885 4917 4959 4991 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2576 645 135 42 32 32 32
32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KasI G'GCGC_C
Cuts at: 2102 2102
Size: 6244
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3018 3843 3018
Size: 825 5419
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2912 5358 5542 1305
Size: 1607 2446 184 2007
Fragments arranged by size:
2446 2007 1607 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2700 2963 10
Size: 54 1425 343 868 263 3291
Fragments arranged by size:
3291 1425 868 343 263 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2541 3425 3491 4202 4561 4720
Size: 648 1092 283 884 66 711 359 159
Cuts at: 4720 518
Size: 2042
Fragments arranged by size:
2042 1092 884 711 648 359 283 159
66
MspA1I CmG'CkG
Cuts at: 1606 2085 3474 3660 4337 5485 5730 1606
Size: 479 1389 186 677 1148 245 2120
Fragments arranged by size:
2120 1389 1148 677 479 245 186
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 6244
NarI GG'CG_CC
Cuts at: 2103 2103
Size: 6244
NcoI C'CATG_G
Cuts at: 513 1065 2022 3317 3420 513
Size: 552 957 1295 103 3337
Fragments arranged by size:
3337 1295 957 552 103
NdeI CA'TA_TG
Cuts at: 387 3229 387
Size: 2842 3402
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NruI TCG'CGA
Cuts at: 2135 2135
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 2485 3602 5147 1132
Size: 97 1256 1117 1545 2229
Fragments arranged by size:
2229 1545 1256 1117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6244
PciI A'CATG_T
Cuts at: 2481 5143 2481
Size: 2662 3582
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6244
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2946 4313 4345 5826 5858 2914
Size: 32 1367 32 1481 32 3300
Fragments arranged by size:
3300 1481 1367 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2340 1613
Size: 727 5517
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PstI C_TGCA'G
Cuts at: 830 2811 3596 830
Size: 1981 785 3478
Fragments arranged by size:
3478 1981 785
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2085 2085
Size: 6244
SacI G_AGCT'C
Cuts at: 2046 2046
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SfcI C'TryA_G
Cuts at: 826 1045 2807 3592 4727 5408 5599 826
Size: 219 1762 785 1135 681 191 1471
Fragments arranged by size:
1762 1471 1135 785 681 219 191
SfoI GGC'GCC
Cuts at: 2104 2104
Size: 6244
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4348 5249 5511 820
Size: 197 926 2405 901 262 1553
Fragments arranged by size:
2405 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3541 4035 493
Size: 3048 494 2702
Fragments arranged by size:
3048 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 2992 3038 4168 5
Size: 47 2940 46 1130 2081
Fragments arranged by size:
2940 2081 1130 47 46
StuI AGG'CCT
Cuts at: 1517 2360 1517
Size: 843 5401
StyI C'CwwG_G
Cuts at: 513 1065 2022 2596 3317 3420 3683 513
Size: 552 957 574 721 103 263 3074
Fragments arranged by size:
3074 957 721 574 552 263 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4271 4288 4441 4626 5071 325
Size: 1255 2691 17 153 185 445 1498
Fragments arranged by size:
2691 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2847 2874 4490
Size: 274 80 33 51 1443 870 27 1616
Cuts at: 4490 6202 96
Size: 1712 138
Fragments arranged by size:
1712 1616 1443 870 274 138 80 51
33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2869 2901 6204 6236 2869
Size: 32 3303 32 2877
Fragments arranged by size:
3303 2877 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2951 1316
Size: 1635 4609
XbaI T'CTAG_A
Cuts at: 3098 3580 3098
Size: 482 5762
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4052 276
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI