Restriction map for
pFN21ASDA0907
(Circular) (Six-base) MAPSORT of: pFN21ASDA0907.seq Check: 2527 from: 1 to: 6421
pFN21ASDA0907 6421 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4231 278
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3714 3764 1937
Size: 1777 50 4594
Fragments arranged by size:
4594 1777 50
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2264 2358 4484 5868 6136 1632
Size: 531 101 94 2126 1384 268 1917
Fragments arranged by size:
2126 1917 1384 531 268 101 94
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 2366 5320 6297 1203
Size: 1163 2954 977 1327
Fragments arranged by size:
2954 1327 1163 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 5150 5150
Size: 6421
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 2630 2876 3608 4164 5736 2630
Size: 246 732 556 1572 3315
Fragments arranged by size:
3315 1572 732 556 246
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 3730 3956 4108 6217 1493
Size: 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2220 2349 3012 4975 160
Size: 872 1188 129 663 1963 1606
Fragments arranged by size:
1963 1606 1188 872 663 129
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 2385 2439 3746 1870
Size: 73 442 54 1307 4545
Fragments arranged by size:
4545 1307 442 73 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3006 3039 3006
Size: 33 6388
BamHI G'GATC_C
Cuts at: 3751 4118 3751
Size: 367 6054
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2061 2162 2825 3742 5197
Size: 325 217 627 274 101 663 917 1455
Cuts at: 5197 618
Size: 1842
Fragments arranged by size:
1842 1455 917 663 627 325 274 217
101
BanII G_rGCy'C
Cuts at: 1391 3740 1391
Size: 2349 4072
BbeI G_GCGC'C
Cuts at: 2065 2065
Size: 6421
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2827 928
Size: 971 928 4522
Fragments arranged by size:
4522 971 928
BbvCI CC'TCA_GC
Cuts at: 3132 3132
Size: 6421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 1903
Size: 34 2675 34 3678
Fragments arranged by size:
3678 2675 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4313 5523 3449
Size: 864 1210 4347
Fragments arranged by size:
4347 1210 864
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6421
BfrBI ATG'CAT
Cuts at: 2707 2707
Size: 6421
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5032 136
Size: 107 122 71 631 605 3360 1525
Fragments arranged by size:
3360 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6416 6416
Size: 6421
BlpI GC'TnA_GC
Cuts at: 3838 3838
Size: 6421
Bme1580I G_kGCm'C
Cuts at: 1790 2165 4482 5638 1790
Size: 375 2317 1156 2573
Fragments arranged by size:
2573 2317 1156 375
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4152 5110 442
Size: 416 53 83 399 24 2735 958 1753
Fragments arranged by size:
2735 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3279 1056
Size: 2223 4198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2070 2073 2652 3219 3243 3255 3487 5081
Size: 831 3 579 567 24 12 232 1594
Cuts at: 5081 1239
Size: 2579
Fragments arranged by size:
2579 1594 831 579 567 232 24 12
3
Bpu10I CC'TnA_GC
Cuts at: 3132 3132
Size: 6421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2382 4546 5411 5709 2382
Size: 2164 865 298 3094
Fragments arranged by size:
3094 2164 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5084 882
Size: 617 3585 2219
Fragments arranged by size:
3585 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3718 4212 493
Size: 711 2514 494 2702
Fragments arranged by size:
2702 2514 711 494
BsaBI GATnn'nnATC
Cuts at: 6415 6415
Size: 6421
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2062 4228 4610 5245
Size: 53 83 186 936 529 2166 382 635
Cuts at: 5245 275
Size: 1451
Fragments arranged by size:
2166 1451 936 635 529 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2550 4854 5252 5526 5673 6084 2550
Size: 2304 398 274 147 411 2887
Fragments arranged by size:
2887 2304 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2158 2188 3652 3682 1178
Size: 30 165 30 755 30 1464 30 3917
Fragments arranged by size:
3917 1464 755 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3060 3120 3434 1186
Size: 1874 60 314 4173
Fragments arranged by size:
4173 1874 314 60
BseYI C'CCAG_C
Cuts at: 3351 3357 3363 3369 3683 5624 6120 3351
Size: 6 6 6 314 1941 496 3652
Fragments arranged by size:
3652 1941 496 314 6 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3284 3546 1163
Size: 573 1548 262 4038
Fragments arranged by size:
4038 1548 573 262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3871 4632 5660 1540
Size: 275 186 1870 761 1028 2301
Fragments arranged by size:
2301 1870 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4482 4567 5638 4482
Size: 85 1071 5265
Fragments arranged by size:
5265 1071 85
BsmI GAATG_Cn'
Cuts at: 2269 3932 4025 2269
Size: 1663 93 4665
Fragments arranged by size:
4665 1663 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3400 3400
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 3740 4482 4567 5638 1391
Size: 399 375 1575 742 85 1071 2174
Fragments arranged by size:
2174 1575 1071 742 399 375 85
BspEI T'CCGG_A
Cuts at: 6084 6084
Size: 6421
BspHI T'CATG_A
Cuts at: 2451 4310 6040 2451
Size: 1859 1730 2832
Fragments arranged by size:
2832 1859 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3776 844
Size: 2932 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4308 1394
Size: 429 2485 3507
Fragments arranged by size:
3507 2485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4916 5090 65
Size: 1183 3668 174 1396
Fragments arranged by size:
3668 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3208 5065 5252 1700
Size: 110 1398 1857 187 2869
Fragments arranged by size:
2869 1857 1398 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6421
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 4481 5493 4481
Size: 1012 5409
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3583 794
Size: 2789 3632
BstBI TT'CG_AA
Cuts at: 2007 5225 2007
Size: 3218 3203
BstEII G'GTnAC_C
Cuts at: 1184 2134 3110 1184
Size: 950 976 4495
Fragments arranged by size:
4495 976 950
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 2578 2233
Size: 345 6076
BstYI r'GATC_y
Cuts at: 1972 3751 4118 4503 4520 5972 6058 6070 6416
Size: 1779 367 385 17 1452 86 12 346
Cuts at: 6416 1972
Size: 1977
Fragments arranged by size:
1977 1779 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3550 3550
Size: 6421
BtgI C'CryG_G
Cuts at: 513 1065 2028 3664 513
Size: 552 963 1636 3270
Fragments arranged by size:
3270 1636 963 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2638 487
Size: 683 56 1412 4270
Fragments arranged by size:
4270 1412 683 56
BtsI GCAGTG_nn'
Cuts at: 2149 3937 4731 4751 2149
Size: 1788 794 20 3819
Fragments arranged by size:
3819 1788 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3725 3725
Size: 6421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5428 809
Size: 507 4112 1802
Fragments arranged by size:
4112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2878 3868 4757
Size: 54 1425 50 275 18 1048 990 889
Cuts at: 4757 6386 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1048 990 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 3398 4351 1353
Size: 755 1290 953 3423
Fragments arranged by size:
3423 1290 953 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5008 5382 5528 387
Size: 1064 138 3419 374 146 1280
Fragments arranged by size:
3419 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 2073 2163 2264 2358 2652 3219
Size: 393 438 3 90 101 94 294 567
Cuts at: 3219 3243 3255 3487 4484 5081 5868 6136 1239
Size: 24 12 232 997 597 787 268 1524
Fragments arranged by size:
1524 997 787 597 567 438 393 294
268 232 101 94 90 24 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2292 3030 2292
Size: 738 5683
EcoO109I rG'GnC_Cy
Cuts at: 3648 3648
Size: 6421
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2625 2707 2625
Size: 82 6339
FspI TGC'GCA
Cuts at: 4927 4927
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 2065 5568 1678
Size: 387 3503 2531
Fragments arranged by size:
3503 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2858 2890 3286 3318 5062
Size: 32 135 32 1286 32 396 32 1744
Cuts at: 5062 5094 5136 5168 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1744 1286 396 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3765 4011 669
Size: 988 281 1827 246 3079
Fragments arranged by size:
3079 1827 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2276 2886 2951 748
Size: 1528 610 65 4218
Fragments arranged by size:
4218 1528 610 65
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KasI G'GCGC_C
Cuts at: 2061 2061
Size: 6421
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2630 5535 5719 1305
Size: 1325 2905 184 2007
Fragments arranged by size:
2905 2007 1325 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4599
Fragments arranged by size:
4599 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 3476 4379 4738 4897 518
Size: 648 1065 1245 903 359 159 2042
Fragments arranged by size:
2042 1245 1065 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2117 2171 2672 3182 3837 4514 5662
Size: 424 87 54 501 510 655 677 1148
Cuts at: 5662 5907 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 655 510 501 424 245
87 54
NaeI GCC'GGC
Cuts at: 3210 3210
Size: 6421
NarI GG'CG_CC
Cuts at: 2062 2062
Size: 6421
NcoI C'CATG_G
Cuts at: 513 1065 3664 513
Size: 552 2599 3270
Fragments arranged by size:
3270 2599 552
NdeI CA'TA_TG
Cuts at: 387 3477 387
Size: 3090 3331
NgoMIV G'CCGG_C
Cuts at: 3208 3208
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 3275 1052
Size: 2223 4198
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NsiI A_TGCA'T
Cuts at: 2709 2709
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 2370 2747 3779 5324 1132
Size: 97 1141 377 1032 1545 2229
Fragments arranged by size:
2229 1545 1141 1032 377 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6421
PciI A'CATG_T
Cuts at: 2366 5320 2366
Size: 2954 3467
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2876 3095 3608 1108
Size: 1768 219 513 3921
Fragments arranged by size:
3921 1768 513 219
PfoI T'CCnGG_A
Cuts at: 2047 2047
Size: 6421
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3648 3648
Size: 6421
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6421
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PspXI vC'TCGA_Gb
Cuts at: 2385 2439 2385
Size: 54 6367
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2733 2765 2733
Size: 32 6389
PstI C_TGCA'G
Cuts at: 830 2431 3773 830
Size: 1601 1342 3478
Fragments arranged by size:
3478 1601 1342
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6421
PvuII CAG'CTG
Cuts at: 2171 2672 3182 2171
Size: 501 510 5410
Fragments arranged by size:
5410 510 501
SacII CC_GC'GG
Cuts at: 2031 2031
Size: 6421
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SexAI A'CCwGG_T
Cuts at: 2325 2325
Size: 6421
SfcI C'TryA_G
Cuts at: 826 1045 2427 3769 4904 5585 5776 826
Size: 219 1382 1342 1135 681 191 1471
Fragments arranged by size:
1471 1382 1342 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2063 2063
Size: 6421
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 3748 1872
Size: 1876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2385 2397 2439 4525 5426 5688
Size: 197 926 442 12 42 2086 901 262
Cuts at: 5688 820
Size: 1553
Fragments arranged by size:
2086 1553 926 901 442 262 197 42
12
SnaBI TAC'GTA
Cuts at: 493 3718 4212 493
Size: 3225 494 2702
Fragments arranged by size:
3225 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 3779 1132
Size: 2647 3774
SspI AAT'ATT
Cuts at: 5 52 4345 5
Size: 47 4293 2081
Fragments arranged by size:
4293 2081 47
StuI AGG'CCT
Cuts at: 1517 2298 2934 1517
Size: 781 636 5004
Fragments arranged by size:
5004 781 636
StyI C'CwwG_G
Cuts at: 513 1065 2187 3664 3860 513
Size: 552 1122 1477 196 3074
Fragments arranged by size:
3074 1477 1122 552 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2665 3287 4448 4465 4618 4803 5248
Size: 1255 1085 622 1161 17 153 185 445
Cuts at: 5248 325
Size: 1498
Fragments arranged by size:
1498 1255 1161 1085 622 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4667 6379 96
Size: 274 80 33 51 1443 2690 1712 138
Fragments arranged by size:
2690 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2088 2120 2158 2190 3652 3684 6381 6413 2088
Size: 32 38 32 1462 32 2697 32 2096
Fragments arranged by size:
2697 2096 1462 38 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3288 1316
Size: 1972 4449
XbaI T'CTAG_A
Cuts at: 2737 2993 3757 2737
Size: 256 764 5401
Fragments arranged by size:
5401 764 256
XhoI C'TCGA_G
Cuts at: 1943 2385 2439 1943
Size: 442 54 5925
Fragments arranged by size:
5925 442 54
XmaI C'CCGG_G
Cuts at: 1870 3746 1870
Size: 1876 4545
XmnI GAAnn'nnTTC
Cuts at: 4550 4550
Size: 6421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4229 276
Size: 53 83 186 936 2695 2468
Fragments arranged by size:
2695 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI PacI
PmlI PspOMI RsrII SacI SanDI SapI SfiI SrfI
SwaI XcmI