Restriction map for
pFN21ASDA0895
(Circular) (Six-base) MAPSORT of: pFN21ASDA0895.seq Check: 1074 from: 1 to: 6136
pFN21ASDA0895 6136 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3198 3479 1937
Size: 1261 281 4594
Fragments arranged by size:
4594 1261 281
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4199 5583 5851 1632
Size: 2567 1384 268 1917
Fragments arranged by size:
2567 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2729 820
Size: 197 1712 4227
Fragments arranged by size:
4227 1712 197
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 4865 4865
Size: 6136
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2091 2477 1184
Size: 907 386 4843
Fragments arranged by size:
4843 907 386
AlwNI CAG_nnn'CTG
Cuts at: 3879 5451 3879
Size: 1572 4564
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 2573 3210 3445 3671 3823 5932 1493
Size: 1080 637 235 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 637 235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2149 2769 3266 3461 1870
Size: 73 206 620 497 195 4545
Fragments arranged by size:
4545 620 497 206 195 73
BamHI G'GATC_C
Cuts at: 3466 3833 3466
Size: 367 5769
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2335 2493 3457 4912 618
Size: 325 217 627 548 158 964 1455 1842
Fragments arranged by size:
1842 1455 964 627 548 325 217 158
BanII G_rGCy'C
Cuts at: 1391 3455 1391
Size: 2064 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2238 928
Size: 971 339 4826
Fragments arranged by size:
4826 971 339
BbvCI CC'TCA_GC
Cuts at: 2526 2855 2526
Size: 329 5807
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4327 4361 1903
Size: 34 2390 34 3678
Fragments arranged by size:
3678 2390 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 2112 1548
Size: 564 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4747 136
Size: 107 122 71 631 605 3075 1525
Fragments arranged by size:
3075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3421 6131 3421
Size: 2710 3426
BlpI GC'TnA_GC
Cuts at: 2700 3553 2700
Size: 853 5283
Bme1580I G_kGCm'C
Cuts at: 1790 4197 5353 1790
Size: 2407 1156 2573
Fragments arranged by size:
2573 2407 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4825 442
Size: 416 53 83 399 24 2450 958 1753
Fragments arranged by size:
2450 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3351 3351
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2390 2864 2885 4796 1239
Size: 809 342 474 21 1911 2579
Fragments arranged by size:
2579 1911 809 474 342 21
Bpu10I CC'TnA_GC
Cuts at: 2064 2526 2855 2064
Size: 462 329 5345
Fragments arranged by size:
5345 462 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4261 5126 5424 4261
Size: 865 298 4973
Fragments arranged by size:
4973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4799 882
Size: 617 3300 2219
Fragments arranged by size:
3300 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2831 3433 3927 493
Size: 711 1627 602 494 2702
Fragments arranged by size:
2702 1627 711 602 494
BsaBI GATnn'nnATC
Cuts at: 3291 6130 3291
Size: 2839 3297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3943 4325 4960 275
Size: 53 83 186 936 2410 382 635 1451
Fragments arranged by size:
2410 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5911
Fragments arranged by size:
5911 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3023 1186
Size: 1837 4299
BseYI C'CCAG_C
Cuts at: 2279 5339 5835 2279
Size: 3060 496 2580
Fragments arranged by size:
3060 2580 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2811 1163
Size: 573 1075 4488
Fragments arranged by size:
4488 1075 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4347 5375 1540
Size: 275 186 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4197 4282 5353 4197
Size: 85 1071 4980
Fragments arranged by size:
4980 1071 85
BsmI GAATG_Cn'
Cuts at: 2856 2982 3647 3740 2856
Size: 126 665 93 5252
Fragments arranged by size:
5252 665 126 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3455 4197 4282 5353 1391
Size: 399 1665 742 85 1071 2174
Fragments arranged by size:
2174 1665 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2769 3087 3491 844
Size: 1925 318 404 3489
Fragments arranged by size:
3489 1925 404 318
BsrBI CCG'CTC
Cuts at: 1394 1823 4023 1394
Size: 429 2200 3507
Fragments arranged by size:
3507 2200 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4780 4967 1700
Size: 110 2970 187 2869
Fragments arranged by size:
2970 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 4196 5208 4196
Size: 1012 5124
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6136
BstBI TT'CG_AA
Cuts at: 2007 4940 2007
Size: 2933 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstYI r'GATC_y
Cuts at: 1972 2311 3421 3466 3833 4218 4235 5687 5773
Size: 339 1110 45 367 385 17 1452 86
Cuts at: 5773 5785 6131 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 339 86
45 17 12
BstZ17I GTA'TAC
Cuts at: 3199 3199
Size: 6136
BtgI C'CryG_G
Cuts at: 513 1065 2909 3352 513
Size: 552 1844 443 3297
Fragments arranged by size:
3297 1844 552 443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 2356 2823 3652 4446 4466 2356
Size: 467 829 794 20 4026
Fragments arranged by size:
4026 829 794 467 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3085 3440 3085
Size: 355 5781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3355 3583 4472
Size: 54 1425 50 275 18 1525 228 889
Cuts at: 4472 6101 8
Size: 1629 43
Fragments arranged by size:
1629 1525 1425 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 4066 1353
Size: 1161 1552 3423
Fragments arranged by size:
3423 1552 1161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4723 5097 5243 387
Size: 1064 138 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2390 2864 2885 4199 4796 5583
Size: 393 416 342 474 21 1314 597 787
Cuts at: 5583 5851 1239
Size: 268 1524
Fragments arranged by size:
1524 1314 787 597 474 416 393 342
268 21
EcoO109I rG'GnC_Cy
Cuts at: 2758 3109 3331 2758
Size: 351 222 5563
Fragments arranged by size:
5563 351 222
EcoRI G'AATT_C
Cuts at: 3210 3445 3823 3210
Size: 235 378 5523
Fragments arranged by size:
5523 378 235
EcoRV GAT'ATC
Cuts at: 2434 2434
Size: 6136
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 5283 1678
Size: 3605 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4777 4809 4851 4883 1373
Size: 32 135 32 3205 32 42 32 2626
Fragments arranged by size:
3205 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2051 2539 748
Size: 1303 488 4345
Fragments arranged by size:
4345 1303 488
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2825 2900 5250 5434 1305
Size: 1520 75 2350 184 2007
Fragments arranged by size:
2350 2007 1520 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4314
Fragments arranged by size:
4314 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 3389 4094 4453 4612 518
Size: 648 873 1350 705 359 159 2042
Fragments arranged by size:
2042 1350 873 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2530 3552 4229 5377 5622 1606
Size: 924 1022 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 924 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2909 3352 513
Size: 552 1844 443 3297
Fragments arranged by size:
3297 1844 552 443
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3494 5039 1132
Size: 97 2265 1545 2229
Fragments arranged by size:
2265 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PmlI CAC'GTG
Cuts at: 2831 2831
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2758 3109 3331 2758
Size: 351 222 5563
Fragments arranged by size:
5563 351 222
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PstI C_TGCA'G
Cuts at: 830 3348 3488 830
Size: 2518 140 3478
Fragments arranged by size:
3478 2518 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2530 2530
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SanDI GG'GwC_CC
Cuts at: 3331 3331
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3348 3488 3348
Size: 140 5996
ScaI AGT'ACT
Cuts at: 2460 4384 6096 2460
Size: 1924 1712 2500
Fragments arranged by size:
2500 1924 1712
SexAI A'CCwGG_T
Cuts at: 3192 3192
Size: 6136
SfcI C'TryA_G
Cuts at: 826 1045 2204 3344 3484 4619 5300 5491 826
Size: 219 1159 1140 140 1135 681 191 1471
Fragments arranged by size:
1471 1159 1140 1135 681 219 191 140
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 4240 5141 5403 820
Size: 197 926 786 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 786 262 197
SnaBI TAC'GTA
Cuts at: 493 3433 3927 493
Size: 2940 494 2702
Fragments arranged by size:
2940 2702 494
SpeI A'CTAG_T
Cuts at: 152 2396 152
Size: 2244 3892
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2909 3352 3575 513
Size: 552 1844 443 223 3074
Fragments arranged by size:
3074 1844 552 443 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2736 4163 4180 4333 4518 4963 325
Size: 1255 1156 1427 17 153 185 445 1498
Fragments arranged by size:
1498 1427 1255 1156 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2458 4382 6094
Size: 274 80 33 51 1443 481 1924 1712
Cuts at: 6094 96
Size: 138
Fragments arranged by size:
1924 1712 1443 481 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6096 6128 6096
Size: 32 6104
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 2534 3472 2534
Size: 938 5198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2916 2916
Size: 6136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 2052 2134 4265 2052
Size: 82 2131 3923
Fragments arranged by size:
3923 2131 82
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SapI SfiI SfoI SrfI SwaI