Restriction map for
pFN21ASDA0886
(Circular) (Six-base) MAPSORT of: pFN21ASDA0886.seq Check: 1454 from: 1 to: 8305
pFN21ASDA0886 8305 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6115 278
Size: 53 83 186 936 4579 2468
Fragments arranged by size:
4579 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2366 5648 1937
Size: 429 3282 4594
Fragments arranged by size:
4594 3282 429
Acc65I G'GTAC_C
Cuts at: 5626 5626
Size: 8305
AclI AA'CG_TT
Cuts at: 1563 4595 6432 6805 1563
Size: 3032 1837 373 3063
Fragments arranged by size:
3063 3032 1837 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2826 3090 3468 3684 3933 5016 5280 5399
Size: 1194 264 378 216 249 1083 264 119
Cuts at: 5399 5452 6368 7752 8020 1632
Size: 53 916 1384 268 1917
Fragments arranged by size:
1917 1384 1194 1083 916 378 268 264
264 249 216 119 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8108
AflIII A'CryG_T
Cuts at: 1203 3410 7204 8181 1203
Size: 2207 3794 977 1327
Fragments arranged by size:
3794 2207 1327 977
AgeI A'CCGG_T
Cuts at: 7136 7136
Size: 8305
AhdI GACnn_n'nnGTC
Cuts at: 7034 7034
Size: 8305
AleI CACnn'nnGTG
Cuts at: 1166 4713 1166
Size: 3547 4758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3826 4781 1184
Size: 2642 955 4708
Fragments arranged by size:
4708 2642 955
AlwNI CAG_nnn'CTG
Cuts at: 2957 3437 4208 6048 7620 2957
Size: 480 771 1840 1572 3642
Fragments arranged by size:
3642 1840 1572 771 480
ApaLI G'TGCA_C
Cuts at: 6362 7518 6362
Size: 1156 7149
ApoI r'AATT_y
Cuts at: 1493 3883 4503 5614 5840 5992 8101 1493
Size: 2390 620 1111 226 152 2109 1697
Fragments arranged by size:
2390 2109 1697 1111 620 226 152
AscI GG'CGCG_CC
Cuts at: 2247 2247
Size: 8305
AseI AT'TA_AT
Cuts at: 160 1032 4071 6859 160
Size: 872 3039 2788 1606
Fragments arranged by size:
3039 2788 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8305
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2292 2350 2566 5630 1870
Size: 73 171 178 58 216 3064 4545
Fragments arranged by size:
4545 3064 216 178 171 73 58
AvrII C'CTAG_G
Cuts at: 2978 2978
Size: 8305
BamHI G'GATC_C
Cuts at: 5635 6002 5635
Size: 367 7938
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 5087 5343 5417 5626
Size: 325 217 627 500 2800 256 74 209
Cuts at: 5626 7081 618
Size: 1455 1842
Fragments arranged by size:
2800 1842 1455 627 500 325 256 217
209 74
BanII G_rGCy'C
Cuts at: 1391 2115 2357 2480 2493 2564 2573 4527 5624
Size: 724 242 123 13 71 9 1954 1097
Cuts at: 5624 1391
Size: 4072
Fragments arranged by size:
4072 1954 1097 724 242 123 71 13
9
BbeI G_GCGC'C
Cuts at: 2291 5347 2291
Size: 3056 5249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 2169 3096 5075 928
Size: 971 135 135 927 1979 4158
Fragments arranged by size:
4158 1979 971 927 135 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6496 6530 1903
Size: 34 4559 34 3678
Fragments arranged by size:
4559 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4714 6197 7407 4714
Size: 1483 1210 5612
Fragments arranged by size:
5612 1483 1210
BclI T'GATC_A
Cuts at: 1548 4210 4644 1548
Size: 2662 434 5209
Fragments arranged by size:
5209 2662 434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6916 136
Size: 107 122 71 631 605 5244 1525
Fragments arranged by size:
5244 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8300 8300
Size: 8305
BlpI GC'TnA_GC
Cuts at: 5722 5722
Size: 8305
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2376 6366 7522 1790
Size: 439 147 3990 1156 2573
Fragments arranged by size:
3990 2573 1156 439 147
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2216 3360 6036
Size: 416 53 83 399 24 799 1144 2676
Cuts at: 6036 6994 442
Size: 958 1753
Fragments arranged by size:
2676 1753 1144 958 799 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2198 2207 3267 3660 4863 5074 5094
Size: 950 9 9 1060 393 1203 211 20
Cuts at: 5094 6965 1239
Size: 1871 2579
Fragments arranged by size:
2579 1871 1203 1060 950 393 211 20
9 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3329 4887 6430 7295 7593 3329
Size: 1558 1543 865 298 4041
Fragments arranged by size:
4041 1558 1543 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2056 2576 2854 6968 882
Size: 617 557 520 278 4114 2219
Fragments arranged by size:
4114 2219 617 557 520 278
BsaAI yAC'GTr
Cuts at: 493 1204 5602 6096 493
Size: 711 4398 494 2702
Fragments arranged by size:
4398 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4996 5514 8299 4996
Size: 518 2785 5002
Fragments arranged by size:
5002 2785 518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2288 5344 6112 6494
Size: 53 83 186 936 755 3056 768 382
Cuts at: 6494 7129 275
Size: 635 1451
Fragments arranged by size:
3056 1451 936 768 755 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4159 6738 7136 7410 7557 7968 4159
Size: 2579 398 274 147 411 4496
Fragments arranged by size:
4496 2579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3400 3430 4081 4111 1178
Size: 30 165 30 1997 30 651 30 5372
Fragments arranged by size:
5372 1997 651 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2034 2138 2150 2153 2162 2186 2448 2554
Size: 848 104 12 3 9 24 262 106
Cuts at: 2554 2617 2730 4535 5291 1186
Size: 63 113 1805 756 4200
Fragments arranged by size:
4200 1805 848 756 262 113 106 104
63 24 12 9 3
BseYI C'CCAG_C
Cuts at: 3427 3923 4742 7508 8004 3427
Size: 496 819 2766 496 3728
Fragments arranged by size:
3728 2766 819 496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5308 1163
Size: 573 3572 4160
Fragments arranged by size:
4160 3572 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 2310 2370 2544 5755 6516
Size: 275 186 77 232 60 174 3211 761
Cuts at: 6516 7544 1540
Size: 1028 2301
Fragments arranged by size:
3211 2301 1028 761 275 232 186 174
77 60
BsiHKAI G_wGCw'C
Cuts at: 3418 4527 4869 6366 6451 7522 3418
Size: 1109 342 1497 85 1071 4201
Fragments arranged by size:
4201 1497 1109 1071 342 85
BsmI GAATG_Cn'
Cuts at: 5816 5909 5816
Size: 93 8212
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2229 2357 2376 2480 2493 2564
Size: 399 325 114 128 19 104 13 71
Cuts at: 2564 2573 3418 4527 4869 5624 6366 6451 7522
Size: 9 845 1109 342 755 742 85 1071
Cuts at: 7522 1391
Size: 2174
Fragments arranged by size:
2174 1109 1071 845 755 742 399 342
325 128 114 104 85 71 19 13
9
BspEI T'CCGG_A
Cuts at: 4159 7968 4159
Size: 3809 4496
BspHI T'CATG_A
Cuts at: 6194 7924 6194
Size: 1730 6575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5660 844
Size: 4816 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2312 2332 6192 1394
Size: 429 489 20 3860 3507
Fragments arranged by size:
3860 3507 489 429 20
BsrDI GCAATG_nn'
Cuts at: 65 1248 3444 4748 5148 6800 6974 65
Size: 1183 2196 1304 400 1652 174 1396
Fragments arranged by size:
2196 1652 1396 1304 1183 400 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 2284 2305 6949 7136 1700
Size: 110 433 41 21 4644 187 2869
Fragments arranged by size:
4644 2869 433 187 110 41 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8305
BssHII G'CGCG_C
Cuts at: 1925 2247 1925
Size: 322 7983
BssSI C'ACGA_G
Cuts at: 6365 7377 6365
Size: 1012 7293
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2957 794
Size: 2163 6142
BstBI TT'CG_AA
Cuts at: 2007 7109 2007
Size: 5102 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8305
BstYI r'GATC_y
Cuts at: 1972 3475 5635 6002 6387 6404 7856 7942 7954
Size: 1503 2160 367 385 17 1452 86 12
Cuts at: 7954 8300 1972
Size: 346 1977
Fragments arranged by size:
2160 1977 1503 1452 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2424 4401 2424
Size: 1977 6328
BtgI C'CryG_G
Cuts at: 513 1065 2022 2214 513
Size: 552 957 192 6604
Fragments arranged by size:
6604 957 552 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7566
Fragments arranged by size:
7566 683 56
BtsI GCAGTG_nn'
Cuts at: 3153 5394 5821 6615 6635 3153
Size: 2241 427 794 20 4823
Fragments arranged by size:
4823 2241 794 427 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8270
DraI TTT'AAA
Cuts at: 5609 5609
Size: 8305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7312 809
Size: 507 5996 1802
Fragments arranged by size:
5996 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 2307 2466
Size: 54 1425 50 275 18 245 232 159
Cuts at: 2466 2541 4896 5752 6641 8270 8
Size: 75 2355 856 889 1629 43
Fragments arranged by size:
2355 1629 1425 889 856 275 245 232
159 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2075 2307 2541 5752 1537
Size: 275 263 232 234 3211 4090
Fragments arranged by size:
4090 3211 275 263 234 232
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2139 2601 2730 3009 3819 3996 4086
Size: 774 12 462 129 279 810 177 90
Cuts at: 4086 4803 5354 5471 6235 1353
Size: 717 551 117 764 3423
Fragments arranged by size:
3423 810 774 764 717 551 462 279
177 129 117 90 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6892 7266 7412 387
Size: 1064 138 5303 374 146 1280
Fragments arranged by size:
5303 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4525 4525
Size: 8305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2198 2207 2826 3090 3267 3468
Size: 393 557 9 9 619 264 177 201
Cuts at: 3468 3660 3684 3933 4863 5016 5074 5094 5280
Size: 192 24 249 930 153 58 20 186
Cuts at: 5280 5399 5452 6368 6965 7752 8020 1239
Size: 119 53 916 597 787 268 1524
Fragments arranged by size:
1524 930 916 787 619 597 557 393
268 264 249 201 192 186 177 153
119 58 53 24 20 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3468 4404 3468
Size: 936 7369
EcoO109I rG'GnC_Cy
Cuts at: 2297 2347 2297
Size: 50 8255
EcoRI G'AATT_C
Cuts at: 3883 5614 5992 3883
Size: 1731 378 6196
Fragments arranged by size:
6196 1731 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2831 4885 2831
Size: 2054 6251
FspI TGC'GCA
Cuts at: 6811 6811
Size: 8305
HaeII r_GCGC'y
Cuts at: 1678 2291 5347 7452 1678
Size: 613 3056 2105 2531
Fragments arranged by size:
3056 2531 2105 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2681 2713 3240 3272 3390
Size: 32 135 32 1109 32 527 32 118
Cuts at: 3390 3422 4976 5008 5437 5469 6946 6978 7020
Size: 32 1554 32 429 32 1477 32 42
Cuts at: 7020 7052 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 1477 1109 527 429 135 118
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2367 2790 4258 5041 5649 5895
Size: 988 281 429 423 1468 783 608 246
Cuts at: 5895 669
Size: 3079
Fragments arranged by size:
3079 1468 988 783 608 429 423 281
246
HindIII A'AGCT_T
Cuts at: 748 2835 3942 4139 748
Size: 2087 1107 197 4914
Fragments arranged by size:
4914 2087 1107 197
HpaI GTT'AAC
Cuts at: 5895 5895
Size: 8305
KasI G'GCGC_C
Cuts at: 2287 5343 2287
Size: 3056 5249
KpnI G_GTAC'C
Cuts at: 5630 5630
Size: 8305
MfeI C'AATT_G
Cuts at: 4614 5904 4614
Size: 1290 7015
MluI A'CGCG_T
Cuts at: 8181 8181
Size: 8305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7419 7603 1305
Size: 6114 184 2007
Fragments arranged by size:
6114 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4898 10
Size: 54 1425 343 3066 3417
Fragments arranged by size:
3417 3066 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3866 4713 6263 6622 6781 518
Size: 648 2700 847 1550 359 159 2042
Fragments arranged by size:
2700 2042 1550 847 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 2389 5721 6398 7546 7791 1606
Size: 610 173 3332 677 1148 245 2120
Fragments arranged by size:
3332 2120 1148 677 610 245 173
NaeI GCC'GGC
Cuts at: 2245 2286 2307 2245
Size: 41 21 8243
Fragments arranged by size:
8243 41 21
NarI GG'CG_CC
Cuts at: 2288 5344 2288
Size: 3056 5249
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6796
Fragments arranged by size:
6796 957 552
NdeI CA'TA_TG
Cuts at: 387 3997 387
Size: 3610 4695
NgoMIV G'CCGG_C
Cuts at: 2243 2284 2305 2243
Size: 41 21 8243
Fragments arranged by size:
8243 41 21
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8305
NotI GC'GGCC_GC
Cuts at: 5752 5752
Size: 8305
NspI r_CATG'y
Cuts at: 1132 1229 3414 3661 3699 4119 5663 7208 1132
Size: 97 2185 247 38 420 1544 1545 2229
Fragments arranged by size:
2229 2185 1545 1544 420 247 97 38
PacI TTA_AT'TAA
Cuts at: 4128 4128
Size: 8305
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8305
PciI A'CATG_T
Cuts at: 3410 7204 3410
Size: 3794 4511
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8305
PmeI GTTT'AAAC
Cuts at: 5609 5609
Size: 8305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6374 6406 7887 7919 6374
Size: 32 1481 32 6760
Fragments arranged by size:
6760 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2297 2347 2297
Size: 50 8255
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8305
PsiI TTA'TAA
Cuts at: 5875 5875
Size: 8305
PstI C_TGCA'G
Cuts at: 830 2635 3802 3892 4537 5657 830
Size: 1805 1167 90 645 1120 3478
Fragments arranged by size:
3478 1805 1167 1120 645 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8305
SacI G_AGCT'C
Cuts at: 4527 4527
Size: 8305
SacII CC_GC'GG
Cuts at: 2217 2217
Size: 8305
SalI G'TCGA_C
Cuts at: 1936 2365 5647 1936
Size: 429 3282 4594
Fragments arranged by size:
4594 3282 429
SanDI GG'GwC_CC
Cuts at: 2297 2347 2297
Size: 50 8255
SapI GCTCTTCn'nnn_
Cuts at: 2601 4086 2601
Size: 1485 6820
SbfI CC_TGCA'GG
Cuts at: 5657 5657
Size: 8305
ScaI AGT'ACT
Cuts at: 4531 6553 8265 4531
Size: 2022 1712 4571
Fragments arranged by size:
4571 2022 1712
SfcI C'TryA_G
Cuts at: 826 1045 2631 3063 3798 3888 4533 5653 6788
Size: 219 1586 432 735 90 645 1120 1135
Cuts at: 6788 7469 7660 826
Size: 681 191 1471
Fragments arranged by size:
1586 1471 1135 1120 735 681 645 432
219 191 90
SfoI GGC'GCC
Cuts at: 2289 5345 2289
Size: 3056 5249
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8305
SgrAI Cr'CCGG_yG
Cuts at: 1700 2243 2284 1700
Size: 543 41 7721
Fragments arranged by size:
7721 543 41
SmaI CCC'GGG
Cuts at: 1872 2294 5632 1872
Size: 422 3338 4545
Fragments arranged by size:
4545 3338 422
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3308 4902 6409 7310 7572 820
Size: 197 926 1365 1594 1507 901 262 1553
Fragments arranged by size:
1594 1553 1507 1365 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5602 6096 493
Size: 5109 494 2702
Fragments arranged by size:
5109 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8305
SphI G_CATG'C
Cuts at: 1132 5663 1132
Size: 4531 3774
SspI AAT'ATT
Cuts at: 5 52 4069 6229 5
Size: 47 4017 2160 2081
Fragments arranged by size:
4017 2160 2081 47
StuI AGG'CCT
Cuts at: 1517 3669 1517
Size: 2152 6153
StyI C'CwwG_G
Cuts at: 513 1065 2022 2776 2978 5154 5744 513
Size: 552 957 754 202 2176 590 3074
Fragments arranged by size:
3074 2176 957 754 590 552 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3512 5250 6332 6349 6502 6687 7132
Size: 1255 1932 1738 1082 17 153 185 445
Cuts at: 7132 325
Size: 1498
Fragments arranged by size:
1932 1738 1498 1255 1082 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3685 4529 5049
Size: 274 80 33 51 1443 1708 844 520
Cuts at: 5049 5292 6551 8263 96
Size: 243 1259 1712 138
Fragments arranged by size:
1712 1708 1443 1259 844 520 274 243
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3400 3432 8265 8297 3400
Size: 32 4833 32 3408
Fragments arranged by size:
4833 3408 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8305
XbaI T'CTAG_A
Cuts at: 5641 5641
Size: 8305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 8305
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8305
XmaI C'CCGG_G
Cuts at: 1870 2292 5630 1870
Size: 422 3338 4545
Fragments arranged by size:
4545 3338 422
XmnI GAAnn'nnTTC
Cuts at: 6434 6434
Size: 8305
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6113 276
Size: 53 83 186 936 4579 2468
Fragments arranged by size:
4579 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PacI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI
FspAI NruI NsiI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SexAI SfiI SrfI SwaI