Restriction map for
pFN21ASDA0885
(Circular) (Six-base) MAPSORT of: pFN21ASDA0885.seq Check: 8711 from: 1 to: 7123
pFN21ASDA0885 7123 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4933 278
Size: 53 83 186 936 3397 2468
Fragments arranged by size:
3397 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4466 1937
Size: 2529 4594
Acc65I G'GTAC_C
Cuts at: 2067 4444 2067
Size: 2377 4746
AclI AA'CG_TT
Cuts at: 1563 2469 5250 5623 1563
Size: 906 2781 373 3063
Fragments arranged by size:
3063 2781 906 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2067 2331 2472 3133 3620 5186 6570 6838
Size: 435 264 141 661 487 1566 1384 268
Cuts at: 6838 1632
Size: 1917
Fragments arranged by size:
1917 1566 1384 661 487 435 268 264
141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6926
AflIII A'CryG_T
Cuts at: 1203 6022 6999 1203
Size: 4819 977 1327
Fragments arranged by size:
4819 1327 977
AgeI A'CCGG_T
Cuts at: 5954 5954
Size: 7123
AhdI GACnn_n'nnGTC
Cuts at: 5852 5852
Size: 7123
AleI CACnn'nnGTG
Cuts at: 1166 2661 3150 1166
Size: 1495 489 5139
Fragments arranged by size:
5139 1495 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2567 1184
Size: 1383 5740
AlwNI CAG_nnn'CTG
Cuts at: 4218 4866 6438 4218
Size: 648 1572 4903
Fragments arranged by size:
4903 1572 648
ApaI G_GGCC'C
Cuts at: 4247 4247
Size: 7123
ApaLI G'TGCA_C
Cuts at: 5180 6336 5180
Size: 1156 5967
ApoI r'AATT_y
Cuts at: 1493 2231 2666 2711 3156 3491 4432 4658 4810
Size: 738 435 45 445 335 941 226 152
Cuts at: 4810 6919 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 941 738 445 435 335 226
152 45
AseI AT'TA_AT
Cuts at: 160 1032 5677 160
Size: 872 4645 1606
Fragments arranged by size:
4645 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
AvaI C'yCGr_G
Cuts at: 1870 1943 2380 3063 4448 1870
Size: 73 437 683 1385 4545
Fragments arranged by size:
4545 1385 683 437 73
BamHI G'GATC_C
Cuts at: 4453 4820 4453
Size: 367 6756
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2067 4444 5899 618
Size: 325 217 627 280 2377 1455 1842
Fragments arranged by size:
2377 1842 1455 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 4247 4442 1391
Size: 2856 195 4072
Fragments arranged by size:
4072 2856 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6152
BbvCI CC'TCA_GC
Cuts at: 3384 3384
Size: 7123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5314 5348 1903
Size: 34 3377 34 3678
Fragments arranged by size:
3678 3377 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5015 6225 5015
Size: 1210 5913
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7123
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5734 136
Size: 107 122 71 631 605 4062 1525
Fragments arranged by size:
4062 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7118 7118
Size: 7123
BlpI GC'TnA_GC
Cuts at: 4540 4540
Size: 7123
Bme1580I G_kGCm'C
Cuts at: 1790 3272 3314 4247 5184 6340 1790
Size: 1482 42 933 937 1156 2573
Fragments arranged by size:
2573 1482 1156 937 933 42
BmgBI CAC'GTC
Cuts at: 1318 2996 1318
Size: 1678 5445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2112 2268 2841
Size: 416 53 83 399 24 695 156 573
Cuts at: 2841 3704 4854 5812 442
Size: 863 1150 958 1753
Fragments arranged by size:
1753 1150 958 863 695 573 416 399
156 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2385 2385
Size: 7123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 2505 5783 1239
Size: 1116 150 3278 2579
Fragments arranged by size:
3278 2579 1116 150
Bpu10I CC'TnA_GC
Cuts at: 3384 3384
Size: 7123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2754 5248 6113 6411 2754
Size: 2494 865 298 3466
Fragments arranged by size:
3466 2494 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5786 882
Size: 617 4287 2219
Fragments arranged by size:
4287 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4420 4914 493
Size: 711 3216 494 2702
Fragments arranged by size:
3216 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7117 7117
Size: 7123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4930 5312 5947 275
Size: 53 83 186 936 3397 382 635 1451
Fragments arranged by size:
3397 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5556 5954 6228 6375 6786 5556
Size: 398 274 147 411 5893
Fragments arranged by size:
5893 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2211 2241 3471 3501 4155
Size: 30 165 30 808 30 1230 30 654
Cuts at: 4155 4185 1178
Size: 30 4116
Fragments arranged by size:
4116 1230 808 654 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3459 3853 3905 4254 4314 1186
Size: 2273 394 52 349 60 3995
Fragments arranged by size:
3995 2273 394 349 60 52
BseYI C'CCAG_C
Cuts at: 6326 6822 6326
Size: 496 6627
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3768 4231 4401 1163
Size: 573 2032 463 170 3885
Fragments arranged by size:
3885 2032 573 463 170
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4573 5334 6362 1540
Size: 275 186 2572 761 1028 2301
Fragments arranged by size:
2572 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2542 4324 5184 5269 6340 2542
Size: 1782 860 85 1071 3325
Fragments arranged by size:
3325 1782 1071 860 85
BsmI GAATG_Cn'
Cuts at: 4634 4727 4634
Size: 93 7030
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2542 3272 3314 4247 4324 4442 5184
Size: 399 752 730 42 933 77 118 742
Cuts at: 5184 5269 6340 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 933 752 742 730 399 118
85 77 42
BspEI T'CCGG_A
Cuts at: 6786 6786
Size: 7123
BspHI T'CATG_A
Cuts at: 3634 5012 6742 3634
Size: 1378 1730 4015
Fragments arranged by size:
4015 1730 1378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 3517 4478 844
Size: 1364 1309 961 3489
Fragments arranged by size:
3489 1364 1309 961
BsrBI CCG'CTC
Cuts at: 1394 1823 5010 1394
Size: 429 3187 3507
Fragments arranged by size:
3507 3187 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2268 2867 5618 5792 65
Size: 1183 1020 599 2751 174 1396
Fragments arranged by size:
2751 1396 1183 1020 599 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5767 5954 1700
Size: 110 3957 187 2869
Fragments arranged by size:
3957 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7123
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7123
BssSI C'ACGA_G
Cuts at: 5183 6195 5183
Size: 1012 6111
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7123
BstBI TT'CG_AA
Cuts at: 2007 5927 2007
Size: 3920 3203
BstEII G'GTnAC_C
Cuts at: 1184 2944 2960 1184
Size: 1760 16 5347
Fragments arranged by size:
5347 1760 16
BstXI CCAn_nnnn'nTGG
Cuts at: 2054 3451 2054
Size: 1397 5726
BstYI r'GATC_y
Cuts at: 1972 3458 4392 4453 4820 5205 5222 6674 6760
Size: 1486 934 61 367 385 17 1452 86
Cuts at: 6760 6772 7118 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1486 1452 934 385 367 346 86
61 17 12
Bsu36I CC'TnA_GG
Cuts at: 3836 3836
Size: 7123
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6571
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2715 487
Size: 683 56 1489 4895
Fragments arranged by size:
4895 1489 683 56
BtsI GCAGTG_nn'
Cuts at: 2090 4639 5433 5453 2090
Size: 2549 794 20 3760
Fragments arranged by size:
3760 2549 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7088
DraI TTT'AAA
Cuts at: 4427 4427
Size: 7123
DraIII CAC_nnn'GTG
Cuts at: 2096 3775 2096
Size: 1679 5444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3552 6130 809
Size: 507 2236 2578 1802
Fragments arranged by size:
2578 2236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3939 4570 5459
Size: 54 1425 50 275 18 2109 631 889
Cuts at: 5459 7088 8
Size: 1629 43
Fragments arranged by size:
2109 1629 1425 889 631 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4570 1537
Size: 275 2758 4090
Fragments arranged by size:
4090 2758 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5053 1353
Size: 3700 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5710 6084 6230 387
Size: 1064 138 4121 374 146 1280
Fragments arranged by size:
4121 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2331 2355 2472 2505 3133 3620
Size: 393 435 264 24 117 33 628 487
Cuts at: 3620 5186 5783 6570 6838 1239
Size: 1566 597 787 268 1524
Fragments arranged by size:
1566 1524 787 628 597 487 435 393
268 264 117 33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3474 3474
Size: 7123
EcoO109I rG'GnC_Cy
Cuts at: 4243 4244 4243
Size: 1 7122
EcoRI G'AATT_C
Cuts at: 2711 4432 4810 2711
Size: 1721 378 5024
Fragments arranged by size:
5024 1721 378
EcoRV GAT'ATC
Cuts at: 2794 2794
Size: 7123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2183 2695 3595 3664 4132 2183
Size: 512 900 69 468 5174
Fragments arranged by size:
5174 900 512 468 69
FspI TGC'GCA
Cuts at: 4043 5629 4043
Size: 1586 5537
HaeII r_GCGC'y
Cuts at: 1678 4218 6270 1678
Size: 2540 2052 2531
Fragments arranged by size:
2540 2531 2052
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2017 2049 2351 2383 2921
Size: 32 135 32 445 32 302 32 538
Cuts at: 2921 2953 3206 3238 3832 3864 4177 4209 5764
Size: 32 253 32 594 32 313 32 1555
Cuts at: 5764 5796 5838 5870 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1555 594 538 445 313 302 253
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2124 2890 4467 4713 669
Size: 988 281 186 766 1577 246 3079
Fragments arranged by size:
3079 1577 988 766 281 246 186
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7123
HpaI GTT'AAC
Cuts at: 2124 4713 2124
Size: 2589 4534
KpnI G_GTAC'C
Cuts at: 2071 4448 2071
Size: 2377 4746
MfeI C'AATT_G
Cuts at: 3986 4722 3986
Size: 736 6387
MluI A'CGCG_T
Cuts at: 6999 6999
Size: 7123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2417 3875 6237 6421 1305
Size: 1112 1458 2362 184 2007
Fragments arranged by size:
2362 2007 1458 1112 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3941 10
Size: 54 1425 343 2109 3192
Fragments arranged by size:
3192 2109 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2052 2526 2661 3150 3660 5081 5440
Size: 648 886 474 135 489 510 1421 359
Cuts at: 5440 5599 518
Size: 159 2042
Fragments arranged by size:
2042 1421 886 648 510 489 474 359
159 135
MspA1I CmG'CkG
Cuts at: 1606 2375 2476 2577 4539 5216 6364 6609 1606
Size: 769 101 101 1962 677 1148 245 2120
Fragments arranged by size:
2120 1962 1148 769 677 245 101 101
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6571
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7123
NotI GC'GGCC_GC
Cuts at: 4570 4570
Size: 7123
NspI r_CATG'y
Cuts at: 1132 1229 4481 6026 1132
Size: 97 3252 1545 2229
Fragments arranged by size:
3252 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7123
PciI A'CATG_T
Cuts at: 6022 6022
Size: 7123
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7123
PmeI GTTT'AAAC
Cuts at: 4427 4427
Size: 7123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5192 5224 6705 6737 4160
Size: 32 1000 32 1481 32 4546
Fragments arranged by size:
4546 1481 1000 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7123
PsiI TTA'TAA
Cuts at: 4693 4693
Size: 7123
PspOMI G'GGCC_C
Cuts at: 4243 4243
Size: 7123
PstI C_TGCA'G
Cuts at: 830 3963 4475 830
Size: 3133 512 3478
Fragments arranged by size:
3478 3133 512
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7123
PvuII CAG'CTG
Cuts at: 2375 2476 2375
Size: 101 7022
RsrII CG'GwC_CG
Cuts at: 2248 2248
Size: 7123
SalI G'TCGA_C
Cuts at: 1936 4465 1936
Size: 2529 4594
SbfI CC_TGCA'GG
Cuts at: 4475 4475
Size: 7123
ScaI AGT'ACT
Cuts at: 5371 7083 5371
Size: 1712 5411
SfcI C'TryA_G
Cuts at: 826 1045 2659 3959 4154 4471 5606 6287 6478
Size: 219 1614 1300 195 317 1135 681 191
Cuts at: 6478 826
Size: 1471
Fragments arranged by size:
1614 1471 1300 1135 681 317 219 195
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7123
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7123
SmaI CCC'GGG
Cuts at: 1872 2382 4450 1872
Size: 510 2068 4545
Fragments arranged by size:
4545 2068 510
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2769 5227 6128 6390 820
Size: 197 926 826 2458 901 262 1553
Fragments arranged by size:
2458 1553 926 901 826 262 197
SnaBI TAC'GTA
Cuts at: 493 4420 4914 493
Size: 3927 494 2702
Fragments arranged by size:
3927 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7123
SphI G_CATG'C
Cuts at: 1132 4481 1132
Size: 3349 3774
SspI AAT'ATT
Cuts at: 5 52 2980 5047 5
Size: 47 2928 2067 2081
Fragments arranged by size:
2928 2081 2067 47
StuI AGG'CCT
Cuts at: 1517 3517 1517
Size: 2000 5123
StyI C'CwwG_G
Cuts at: 513 1065 2940 3264 3444 3868 4248 4349 4562
Size: 552 1875 324 180 424 380 101 213
Cuts at: 4562 513
Size: 3074
Fragments arranged by size:
3074 1875 552 424 380 324 213 180
101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2265 3006 3062 5150 5167 5320 5505
Size: 1255 685 741 56 2088 17 153 185
Cuts at: 5505 5950 325
Size: 445 1498
Fragments arranged by size:
2088 1498 1255 741 685 445 185 153
56 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5369 7081 96
Size: 274 80 33 51 1443 3392 1712 138
Fragments arranged by size:
3392 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 4155 4187 7083 7115 2319
Size: 32 1804 32 2896 32 2327
Fragments arranged by size:
2896 2327 1804 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3704 1316
Size: 2388 4735
XbaI T'CTAG_A
Cuts at: 4459 4459
Size: 7123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3577 3577
Size: 7123
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7123
XmaI C'CCGG_G
Cuts at: 1870 2380 4448 1870
Size: 510 2068 4545
Fragments arranged by size:
4545 2068 510
XmnI GAAnn'nnTTC
Cuts at: 5252 5252
Size: 7123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4931 276
Size: 53 83 186 936 3397 2468
Fragments arranged by size:
3397 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI