Restriction map for
pFN21ASDA0881
(Circular) (Six-base) MAPSORT of: pFN21ASDA0881.seq Check: 7142 from: 1 to: 7876
pFN21ASDA0881 7876 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2563 2563
Size: 7876
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2646 5686 278
Size: 53 83 186 936 1110 3040 2468
Fragments arranged by size:
3040 2468 1110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2148 2796 5219 1937
Size: 211 648 2423 4594
Fragments arranged by size:
4594 2423 648 211
Acc65I G'GTAC_C
Cuts at: 5197 5197
Size: 7876
AclI AA'CG_TT
Cuts at: 1563 6003 6376 1563
Size: 4440 373 3063
Fragments arranged by size:
4440 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2375 2414 2792 2870 3225 3824 4818
Size: 440 303 39 378 78 355 599 994
Cuts at: 4818 5939 7323 7591 1632
Size: 1121 1384 268 1917
Fragments arranged by size:
1917 1384 1121 994 599 440 378 355
303 268 78 39
AflII C'TTAA_G
Cuts at: 820 1017 4376 820
Size: 197 3359 4320
Fragments arranged by size:
4320 3359 197
AflIII A'CryG_T
Cuts at: 1203 4292 6775 7752 1203
Size: 3089 2483 977 1327
Fragments arranged by size:
3089 2483 1327 977
AgeI A'CCGG_T
Cuts at: 6707 6707
Size: 7876
AhdI GACnn_n'nnGTC
Cuts at: 2647 6605 2647
Size: 3958 3918
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3495 3989 4233 1184
Size: 2311 494 244 4827
Fragments arranged by size:
4827 2311 494 244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2240 2272 3695 3727 4481 4513 2240
Size: 32 1423 32 754 32 5603
Fragments arranged by size:
5603 1423 754 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2360 2437 3541 4003 4468 4531 5038 5619 7191
Size: 77 1104 462 465 63 507 581 1572
Cuts at: 7191 2360
Size: 3045
Fragments arranged by size:
3045 1572 1104 581 507 465 462 77
63
ApaI G_GGCC'C
Cuts at: 4974 5084 5109 4974
Size: 110 25 7741
Fragments arranged by size:
7741 110 25
ApaLI G'TGCA_C
Cuts at: 2375 3585 4053 5933 7089 2375
Size: 1210 468 1880 1156 3162
Fragments arranged by size:
3162 1880 1210 1156 468
ApoI r'AATT_y
Cuts at: 1493 2788 5185 5411 5563 7672 1493
Size: 1295 2397 226 152 2109 1697
Fragments arranged by size:
2397 2109 1697 1295 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6430 160
Size: 872 5398 1606
Fragments arranged by size:
5398 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7876
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 2594 3065 3210 5053 5102 5201
Size: 73 214 437 471 145 1843 49 99
Cuts at: 5201 1870
Size: 4545
Fragments arranged by size:
4545 1843 471 437 214 145 99 73
49
BamHI G'GATC_C
Cuts at: 5206 5573 5206
Size: 367 7509
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 2573 2590 2980 2996
Size: 325 217 627 764 22 17 390 16
Cuts at: 2996 3657 5098 5197 6652 618
Size: 661 1441 99 1455 1842
Fragments arranged by size:
1842 1455 1441 764 661 627 390 325
217 99 22 17 16
BanII G_rGCy'C
Cuts at: 1391 2076 2354 3033 3287 3816 4137 4974 5008
Size: 685 278 679 254 529 321 837 34
Cuts at: 5008 5084 5109 5195 1391
Size: 76 25 86 4072
Fragments arranged by size:
4072 837 685 679 529 321 278 254
86 76 34 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2798 928
Size: 971 899 6006
Fragments arranged by size:
6006 971 899
BbvCI CC'TCA_GC
Cuts at: 3560 3560
Size: 7876
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5110 5144 6067 6101 1903
Size: 34 3173 34 923 34 3678
Fragments arranged by size:
3678 3173 923 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5768 6978 5768
Size: 1210 6666
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7876
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5041 6487 136
Size: 107 122 71 631 605 3369 1446 1525
Fragments arranged by size:
3369 1525 1446 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7871 7871
Size: 7876
BlpI GC'TnA_GC
Cuts at: 2070 2353 3497 4131 4359 4454 5293 2070
Size: 283 1144 634 228 95 839 4653
Fragments arranged by size:
4653 1144 839 634 283 228 95
Bme1580I G_kGCm'C
Cuts at: 1790 2379 2576 3087 3589 4057 4636 4876 4974
Size: 589 197 511 502 468 579 240 98
Cuts at: 4974 5084 5103 5109 5937 7093 1790
Size: 110 19 6 828 1156 2573
Fragments arranged by size:
2573 1156 828 589 579 511 502 468
240 197 110 98 19 6
BmgBI CAC'GTC
Cuts at: 1318 4293 1318
Size: 2975 4901
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5607 6565 442
Size: 416 53 83 399 24 4190 958 1753
Fragments arranged by size:
4190 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7876
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 7876
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2741 3091 3323 3569 3782 3800 3866 4091
Size: 1502 350 232 246 213 18 66 225
Cuts at: 4091 4112 4196 4361 4553 4628 4661 4753 4899
Size: 21 84 165 192 75 33 92 146
Cuts at: 4899 4900 6536 1239
Size: 1 1636 2579
Fragments arranged by size:
2579 1636 1502 350 246 232 225 213
192 165 146 92 84 75 66 33
21 18 1
Bpu10I CC'TnA_GC
Cuts at: 3560 5111 3560
Size: 1551 6325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2805 3626 4370 4730 6001 6866 7164 2805
Size: 821 744 360 1271 865 298 3517
Fragments arranged by size:
3517 1271 865 821 744 360 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3405 4337 4475 6539 882
Size: 617 1906 932 138 2064 2219
Fragments arranged by size:
2219 2064 1906 932 617 138
BsaAI yAC'GTr
Cuts at: 493 1204 5173 5667 493
Size: 711 3969 494 2702
Fragments arranged by size:
3969 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4565 7870 4565
Size: 3305 4571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2643 4924 5683 6065
Size: 53 83 186 936 1110 2281 759 382
Cuts at: 6065 6700 275
Size: 635 1451
Fragments arranged by size:
2281 1451 1110 936 759 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2166 2784 6309 6707 6981 7128 7539 2166
Size: 618 3525 398 274 147 411 2503
Fragments arranged by size:
3525 2503 618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2253 2270 2283 3695
Size: 30 165 30 837 13 17 13 1412
Cuts at: 3695 3725 4481 4511 1178
Size: 30 756 30 4543
Fragments arranged by size:
4543 1412 837 756 165 30 30 30
30 17 13 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3026 3035 3176 3179 3182 3185 3188 3197
Size: 1840 9 141 3 3 3 3 9
Cuts at: 3197 3200 3203 3590 3716 3824 3914 4673 4884
Size: 3 3 387 126 108 90 759 211
Cuts at: 4884 1186
Size: 4178
Fragments arranged by size:
4178 1840 759 387 211 141 126 108
90 9 9 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2030 3086 3329 3355 3827 4162 4759 4837 4966
Size: 1056 243 26 472 335 597 78 129
Cuts at: 4966 5031 7079 7575 2030
Size: 65 2048 496 2331
Fragments arranged by size:
2331 2048 1056 597 496 472 335 243
129 78 65 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 4136 1163
Size: 573 696 1704 4903
Fragments arranged by size:
4903 1704 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5326 6087 7115 1540
Size: 275 186 3325 761 1028 2301
Fragments arranged by size:
3325 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2319 2379 2757 2868 3243 3582 3589 3816
Size: 243 60 378 111 375 339 7 227
Cuts at: 3816 4057 4137 4333 5937 6022 7093 2076
Size: 241 80 196 1604 85 1071 2859
Fragments arranged by size:
2859 1604 1071 378 375 339 243 241
227 196 111 85 80 60 7
BsmI GAATG_Cn'
Cuts at: 2713 5387 5480 2713
Size: 2674 93 5109
Fragments arranged by size:
5109 2674 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4283 4938 4283
Size: 655 7221
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2319 2354 2379 2576 2757 2868
Size: 399 286 243 35 25 197 181 111
Cuts at: 2868 3033 3087 3243 3287 3582 3589 3816 4057
Size: 165 54 156 44 295 7 227 241
Cuts at: 4057 4137 4333 4636 4876 4974 5008 5084 5103
Size: 80 196 303 240 98 34 76 19
Cuts at: 5103 5109 5195 5937 6022 7093 1391
Size: 6 86 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 295 286 243
241 240 227 197 196 181 165 156
111 98 86 85 80 76 54 44
35 34 25 19 7 6
BspEI T'CCGG_A
Cuts at: 2166 2784 7539 2166
Size: 618 4755 2503
Fragments arranged by size:
4755 2503 618
BspHI T'CATG_A
Cuts at: 5765 7495 5765
Size: 1730 6146
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2563 2672 2949 4011 5231 844
Size: 1719 109 277 1062 1220 3489
Fragments arranged by size:
3489 1719 1220 1062 277 109
BsrBI CCG'CTC
Cuts at: 1394 1823 2695 2875 3501 3706 4714 5763 1394
Size: 429 872 180 626 205 1008 1049 3507
Fragments arranged by size:
3507 1049 1008 872 626 429 205 180
BsrDI GCAATG_nn'
Cuts at: 65 1248 2730 6371 6545 65
Size: 1183 1482 3641 174 1396
Fragments arranged by size:
3641 1482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3273 6520 6707 1700
Size: 110 1463 3247 187 2869
Fragments arranged by size:
3247 2869 1463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7876
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7876
BssSI C'ACGA_G
Cuts at: 3684 4073 4586 5936 6948 3684
Size: 389 513 1350 1012 4612
Fragments arranged by size:
4612 1350 1012 513 389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3689 4204 794
Size: 2895 515 4466
Fragments arranged by size:
4466 2895 515
BstBI TT'CG_AA
Cuts at: 2007 6680 2007
Size: 4673 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7876
BstYI r'GATC_y
Cuts at: 1972 2964 3456 4299 4566 5206 5573 5958 5975
Size: 992 492 843 267 640 367 385 17
Cuts at: 5975 7427 7513 7525 7871 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 992 843 640 492 385 367
346 267 86 17 12
BstZ17I GTA'TAC
Cuts at: 2149 2149
Size: 7876
Bsu36I CC'TnA_GG
Cuts at: 2309 2309
Size: 7876
BtgI C'CryG_G
Cuts at: 513 1065 2128 2419 2610 4885 4996 5094 513
Size: 552 1063 291 191 2275 111 98 3295
Fragments arranged by size:
3295 2275 1063 552 291 191 111 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2726 487
Size: 683 56 1500 5637
Fragments arranged by size:
5637 1500 683 56
BtsI GCAGTG_nn'
Cuts at: 3893 3953 4055 4634 5068 5074 5392 6186 6206
Size: 60 102 579 434 6 318 794 20
Cuts at: 6206 3893
Size: 5563
Fragments arranged by size:
5563 794 579 434 318 102 60 20
6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7876
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7841
DraI TTT'AAA
Cuts at: 5180 5180
Size: 7876
DraIII CAC_nnn'GTG
Cuts at: 3082 4985 4997 3082
Size: 1903 12 5961
Fragments arranged by size:
5961 1903 12
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6883 809
Size: 507 5567 1802
Fragments arranged by size:
5567 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2184 2607
Size: 54 1425 50 275 18 295 59 423
Cuts at: 2607 2876 3219 5323 6212 7841 8
Size: 269 343 2104 889 1629 43
Fragments arranged by size:
2104 1629 1425 889 423 343 295 275
269 59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5323 1537
Size: 275 3511 4090
Fragments arranged by size:
4090 3511 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2108 3012 3153 3174 3382 4386 4650
Size: 728 27 904 141 21 208 1004 264
Cuts at: 4650 5806 1353
Size: 1156 3423
Fragments arranged by size:
3423 1156 1004 904 728 264 208 141
27 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3911 4436 5016 6463 6837 6983
Size: 1064 138 2322 525 580 1447 374 146
Cuts at: 6983 387
Size: 1280
Fragments arranged by size:
2322 1447 1280 1064 580 525 374 146
138
EcoICRI GAG'CTC
Cuts at: 2074 3814 4135 2074
Size: 1740 321 5815
Fragments arranged by size:
5815 1740 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2375 2414 2741 2792 2870 3091
Size: 393 440 303 39 327 51 78 221
Cuts at: 3091 3225 3323 3569 3782 3800 3824 3866 4091
Size: 134 98 246 213 18 24 42 225
Cuts at: 4091 4112 4196 4361 4553 4628 4661 4753 4818
Size: 21 84 165 192 75 33 92 65
Cuts at: 4818 4899 4900 5939 6536 7323 7591 1239
Size: 81 1 1039 597 787 268 1524
Fragments arranged by size:
1524 1039 787 597 440 393 327 303
268 246 225 221 213 192 165 134
98 92 84 81 78 75 65 51
42 39 33 24 21 18 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2393 3761 3995 4899 2393
Size: 1368 234 904 5370
Fragments arranged by size:
5370 1368 904 234
EcoO109I rG'GnC_Cy
Cuts at: 2056 2434 3004 3200 4000 4015 4504 4952 4970
Size: 378 570 196 800 15 489 448 18
Cuts at: 4970 4971 5081 5106 2056
Size: 1 110 25 4826
Fragments arranged by size:
4826 800 570 489 448 378 196 110
25 18 15 1
EcoRI G'AATT_C
Cuts at: 5185 5563 5185
Size: 378 7498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 7876
FspI TGC'GCA
Cuts at: 6382 6382
Size: 7876
HaeII r_GCGC'y
Cuts at: 1678 2429 3105 3931 4469 7023 1678
Size: 751 676 826 538 2554 2531
Fragments arranged by size:
2554 2531 826 751 676 538
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 4291 4323 6517
Size: 32 135 32 1061 32 1626 32 2194
Cuts at: 6517 6549 6591 6623 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2194 1626 1061 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 3127 5220 5466 669
Size: 988 281 859 330 2093 246 3079
Fragments arranged by size:
3079 2093 988 859 330 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7876
HpaI GTT'AAC
Cuts at: 5466 5466
Size: 7876
KpnI G_GTAC'C
Cuts at: 5201 5201
Size: 7876
MfeI C'AATT_G
Cuts at: 5475 5475
Size: 7876
MluI A'CGCG_T
Cuts at: 7752 7752
Size: 7876
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6990 7174 1305
Size: 5685 184 2007
Fragments arranged by size:
5685 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2127 2186 2609 3221 10
Size: 54 1425 343 295 59 423 612 4665
Fragments arranged by size:
4665 1425 612 423 343 295 59 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2472 5834 6193 6352 518
Size: 648 1306 3362 359 159 2042
Fragments arranged by size:
3362 2042 1306 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2411 3496 3538 3598 3616 3802 4174
Size: 424 381 1085 42 60 18 186 372
Cuts at: 4174 4648 5096 5292 5969 7117 7362 1606
Size: 474 448 196 677 1148 245 2120
Fragments arranged by size:
2120 1148 1085 677 474 448 424 381
372 245 196 186 60 42 18
NcoI C'CATG_G
Cuts at: 513 1065 2128 2610 4885 513
Size: 552 1063 482 2275 3504
Fragments arranged by size:
3504 2275 1063 552 482
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7876
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7876
NotI GC'GGCC_GC
Cuts at: 5323 5323
Size: 7876
NspI r_CATG'y
Cuts at: 1132 1229 3040 3094 4220 4909 5234 6779 1132
Size: 97 1811 54 1126 689 325 1545 2229
Fragments arranged by size:
2229 1811 1545 1126 689 325 97 54
PasI CC'CwG_GG
Cuts at: 1348 4810 1348
Size: 3462 4414
PciI A'CATG_T
Cuts at: 6775 6775
Size: 7876
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7876
PfoI T'CCnGG_A
Cuts at: 2115 2868 3459 3816 4284 2115
Size: 753 591 357 468 5707
Fragments arranged by size:
5707 753 591 468 357
PmeI GTTT'AAAC
Cuts at: 5180 5180
Size: 7876
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2240 2272 3695 3727 4481 4513 5945 5977 7458
Size: 32 1423 32 754 32 1432 32 1481
Cuts at: 7458 7490 2240
Size: 32 2626
Fragments arranged by size:
2626 1481 1432 1423 754 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 4000 4015 2056
Size: 1944 15 5917
Fragments arranged by size:
5917 1944 15
PshAI GACnn'nnGTC
Cuts at: 1613 2770 2802 1613
Size: 1157 32 6687
Fragments arranged by size:
6687 1157 32
PsiI TTA'TAA
Cuts at: 5446 5446
Size: 7876
PspOMI G'GGCC_C
Cuts at: 4970 5080 5105 4970
Size: 110 25 7741
Fragments arranged by size:
7741 110 25
PstI C_TGCA'G
Cuts at: 830 2946 3352 3597 3948 4149 4269 4677 5228
Size: 2116 406 245 351 201 120 408 551
Cuts at: 5228 830
Size: 3478
Fragments arranged by size:
3478 2116 551 408 406 351 245 201
120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7876
PvuII CAG'CTG
Cuts at: 2030 2411 3496 3538 3802 4174 2030
Size: 381 1085 42 264 372 5732
Fragments arranged by size:
5732 1085 381 372 264 42
SacI G_AGCT'C
Cuts at: 2076 3816 4137 2076
Size: 1740 321 5815
Fragments arranged by size:
5815 1740 321
SacII CC_GC'GG
Cuts at: 5097 5097
Size: 7876
SalI G'TCGA_C
Cuts at: 1936 2795 5218 1936
Size: 859 2423 4594
Fragments arranged by size:
4594 2423 859
SapI GCTCTTCn'nnn_
Cuts at: 2081 2108 4386 2081
Size: 27 2278 5571
Fragments arranged by size:
5571 2278 27
SbfI CC_TGCA'GG
Cuts at: 5228 5228
Size: 7876
ScaI AGT'ACT
Cuts at: 2780 3935 6124 7836 2780
Size: 1155 2189 1712 2820
Fragments arranged by size:
2820 2189 1712 1155
SfcI C'TryA_G
Cuts at: 826 1045 2203 2942 3348 3593 3944 4145 4265
Size: 219 1158 739 406 245 351 201 120
Cuts at: 4265 4673 5224 6359 7040 7231 826
Size: 408 551 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 739 681 551 408 406
351 245 219 201 191 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7876
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7876
SmaI CCC'GGG
Cuts at: 1872 2159 3212 5104 5203 1872
Size: 287 1053 1892 99 4545
Fragments arranged by size:
4545 1892 1053 287 99
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2820 3065 3605 4376 4385 4709
Size: 197 926 877 245 540 771 9 324
Cuts at: 4709 5980 6881 7143 820
Size: 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 877 771 540 324
262 245 197 9
SnaBI TAC'GTA
Cuts at: 493 5173 5667 493
Size: 4680 494 2702
Fragments arranged by size:
4680 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7876
SphI G_CATG'C
Cuts at: 1132 4220 4909 5234 1132
Size: 3088 689 325 3774
Fragments arranged by size:
3774 3088 689 325
SrfI GCCC'GGGC
Cuts at: 5104 5104
Size: 7876
SspI AAT'ATT
Cuts at: 5 52 5800 5
Size: 47 5748 2081
Fragments arranged by size:
5748 2081 47
StuI AGG'CCT
Cuts at: 1517 4736 1517
Size: 3219 4657
StyI C'CwwG_G
Cuts at: 513 1065 2128 2610 2652 2913 3727 4885 5315
Size: 552 1063 482 42 261 814 1158 430
Cuts at: 5315 513
Size: 3074
Fragments arranged by size:
3074 1158 1063 814 552 482 430 261
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2896 2998 5903 5920 6073 6258 6703
Size: 1255 1316 102 2905 17 153 185 445
Cuts at: 6703 325
Size: 1498
Fragments arranged by size:
2905 1498 1316 1255 445 185 153 102
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 3933 3957
Size: 274 80 33 51 1443 801 1155 24
Cuts at: 3957 4179 4314 6122 7834 96
Size: 222 135 1808 1712 138
Fragments arranged by size:
1808 1712 1443 1155 801 274 222 138
135 80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4034 4066 4981 5013 5150 5182 7836 7868 4034
Size: 32 915 32 137 32 2654 32 4042
Fragments arranged by size:
4042 2654 915 137 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7876
XbaI T'CTAG_A
Cuts at: 2368 5212 2368
Size: 2844 5032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2135 2566 2890 4087 4777 5144 2135
Size: 431 324 1197 690 367 4867
Fragments arranged by size:
4867 1197 690 431 367 324
XhoI C'TCGA_G
Cuts at: 1943 3065 1943
Size: 1122 6754
XmaI C'CCGG_G
Cuts at: 1870 2157 3210 5102 5201 1870
Size: 287 1053 1892 99 4545
Fragments arranged by size:
4545 1892 1053 287 99
XmnI GAAnn'nnTTC
Cuts at: 4280 6005 4280
Size: 1725 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2644 5684 276
Size: 53 83 186 936 1110 3040 2468
Fragments arranged by size:
3040 2468 1110 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI
SfiI SfoI SwaI