Restriction map for
pFN21ASDA0873
(Circular) (Six-base) MAPSORT of: pFN21ASDA0873.seq Check: 3981 from: 1 to: 6070
pFN21ASDA0873 6070 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 2095 2217 4133 5517 5785 1632
Size: 413 50 122 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 413 268 122 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 2428 4969 5946 1203
Size: 1225 2541 977 1327
Fragments arranged by size:
2541 1327 1225 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 2250 4799 2250
Size: 2549 3521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 3813 5385 3813
Size: 1572 4498
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 3379 3605 3757 5866 1493
Size: 1886 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2610 2683 3395 1870
Size: 73 667 73 712 4545
Fragments arranged by size:
4545 712 667 73 73
BamHI G'GATC_C
Cuts at: 3400 3767 3400
Size: 367 5703
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3391 4846 618
Size: 325 217 627 1604 1455 1842
Fragments arranged by size:
1842 1604 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 3389 1391
Size: 1548 450 4072
Fragments arranged by size:
4072 1548 450
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2520 928
Size: 971 347 274 4478
Fragments arranged by size:
4478 971 347 274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 3048 1548
Size: 1500 4570
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4681 136
Size: 107 122 71 631 605 3009 1525
Fragments arranged by size:
3009 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 4131 5287 1790
Size: 2341 1156 2573
Fragments arranged by size:
2573 2341 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2320 3801 4759
Size: 416 53 83 399 24 903 1481 958
Cuts at: 4759 442
Size: 1753
Fragments arranged by size:
1753 1481 958 903 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3245 1056
Size: 2189 3881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2460 4730 1239
Size: 929 292 2270 2579
Fragments arranged by size:
2579 2270 929 292
Bpu10I CC'TnA_GC
Cuts at: 2933 2933
Size: 6070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3098 4195 5060 5358 2855
Size: 243 1097 865 298 3567
Fragments arranged by size:
3567 1097 865 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2648 4733 882
Size: 617 1149 2085 2219
Fragments arranged by size:
2219 2085 1149 617
BsaAI yAC'GTr
Cuts at: 493 1204 3367 3861 493
Size: 711 2163 494 2702
Fragments arranged by size:
2702 2163 711 494
BsaBI GATnn'nnATC
Cuts at: 2559 3186 6064 2559
Size: 627 2878 2565
Fragments arranged by size:
2878 2565 627
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3877 4259 4894 275
Size: 53 83 186 936 2344 382 635 1451
Fragments arranged by size:
2344 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2292 4503 4901 5175 5322 5733 2292
Size: 2211 398 274 147 411 2629
Fragments arranged by size:
2629 2211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2921 2951 1178
Size: 30 165 30 1518 30 4297
Fragments arranged by size:
4297 1518 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3119 1186
Size: 1933 4137
BseYI C'CCAG_C
Cuts at: 5273 5769 5273
Size: 496 5574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 3520 4281 5309 1540
Size: 275 186 724 795 761 1028 2301
Fragments arranged by size:
2301 1028 795 761 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 2682 2939 3111 4131 4216 5287 2682
Size: 257 172 1020 85 1071 3465
Fragments arranged by size:
3465 1071 1020 257 172 85
BsmI GAATG_Cn'
Cuts at: 2281 3581 3674 2281
Size: 1300 93 4677
Fragments arranged by size:
4677 1300 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2682 2939 3111 3389 4131 4216 5287
Size: 399 892 257 172 278 742 85 1071
Cuts at: 5287 1391
Size: 2174
Fragments arranged by size:
2174 1071 892 742 399 278 257 172
85
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2666 3425 844
Size: 1822 759 3489
Fragments arranged by size:
3489 1822 759
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 2333 4130 5142 2333
Size: 1797 1012 3261
Fragments arranged by size:
3261 1797 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 2007 4874 2007
Size: 2867 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2029
Size: 6070
BstYI r'GATC_y
Cuts at: 1972 3156 3400 3767 4152 4169 5621 5707 5719
Size: 1184 244 367 385 17 1452 86 12
Cuts at: 5719 6065 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1184 385 367 346 244 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4561
Fragments arranged by size:
4561 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 3033 3586 4380 4400 3033
Size: 553 794 20 4703
Fragments arranged by size:
4703 794 553 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 2853 3374 2853
Size: 521 5549
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2722 3517 4406
Size: 54 1425 50 275 18 892 795 889
Cuts at: 4406 6035 8
Size: 1629 43
Fragments arranged by size:
1629 1425 892 889 795 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2722 3517 1537
Size: 275 910 795 4090
Fragments arranged by size:
4090 910 795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 3016 3213 3267 4000 1353
Size: 1440 223 197 54 733 3423
Fragments arranged by size:
3423 1440 733 223 197 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2937 2937
Size: 6070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2095 2168 2217 2460 4133 4730
Size: 393 413 50 73 49 243 1673 597
Cuts at: 4730 5517 5785 1239
Size: 787 268 1524
Fragments arranged by size:
1673 1524 787 597 413 393 268 243
73 50 49
EcoO109I rG'GnC_Cy
Cuts at: 2617 2866 3073 2617
Size: 249 207 5614
Fragments arranged by size:
5614 249 207
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
EcoRV GAT'ATC
Cuts at: 2410 2767 2410
Size: 357 5713
FspI TGC'GCA
Cuts at: 3315 4576 3315
Size: 1261 4809
HaeII r_GCGC'y
Cuts at: 1678 5217 1678
Size: 3539 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2236 2268 2433 2465 2824
Size: 32 135 32 664 32 165 32 359
Cuts at: 2824 2856 3015 3047 3098 3130 3329 3361 4711
Size: 32 159 32 51 32 199 32 1350
Cuts at: 4711 4743 4785 4817 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 664 359 199 165 159 135
51 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2247 2952 5184 5368 1305
Size: 942 705 2232 184 2007
Fragments arranged by size:
2232 2007 942 705 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4248
Fragments arranged by size:
4248 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 4028 4387 4546 518
Size: 648 861 2001 359 159 2042
Fragments arranged by size:
2042 2001 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3238 3486 4163 5311 5556 1606
Size: 1632 248 677 1148 245 2120
Fragments arranged by size:
2120 1632 1148 677 248 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4561
Fragments arranged by size:
4561 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 3241 1052
Size: 2189 3881
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2432 3428 4973 1132
Size: 97 1203 996 1545 2229
Fragments arranged by size:
2229 1545 1203 996 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 2428 4969 2428
Size: 2541 3529
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6070
PfoI T'CCnGG_A
Cuts at: 3006 3006
Size: 6070
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2241 2273 4139 4171 5652 5684 2241
Size: 32 1866 32 1481 32 2627
Fragments arranged by size:
2627 1866 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2617 2866 2617
Size: 249 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 2361 3640 2361
Size: 1279 4791
PstI C_TGCA'G
Cuts at: 830 2663 3422 830
Size: 1833 759 3478
Fragments arranged by size:
3478 1833 759
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 3238 3238
Size: 6070
SacI G_AGCT'C
Cuts at: 2939 2939
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SapI GCTCTTCn'nnn_
Cuts at: 3016 3213 3016
Size: 197 5873
SbfI CC_TGCA'GG
Cuts at: 2663 3422 2663
Size: 759 5311
ScaI AGT'ACT
Cuts at: 3362 4318 6030 3362
Size: 956 1712 3402
Fragments arranged by size:
3402 1712 956
SfcI C'TryA_G
Cuts at: 826 1045 2187 2659 3418 4553 5234 5425 826
Size: 219 1142 472 759 1135 681 191 1471
Fragments arranged by size:
1471 1142 1135 759 681 472 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 3397 1872
Size: 1525 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2870 3077 4174 5075 5337 820
Size: 197 926 927 207 1097 901 262 1553
Fragments arranged by size:
1553 1097 927 926 901 262 207 197
SnaBI TAC'GTA
Cuts at: 493 3367 3861 493
Size: 2874 494 2702
Fragments arranged by size:
2874 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 3428 1132
Size: 2296 3774
SspI AAT'ATT
Cuts at: 5 52 3322 3994 5
Size: 47 3270 672 2081
Fragments arranged by size:
3270 2081 672 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2022 3139 3509 513
Size: 552 957 1117 370 3074
Fragments arranged by size:
3074 1117 957 552 370
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2301 2627 3126 4097 4114 4267 4452
Size: 1255 721 326 499 971 17 153 185
Cuts at: 4452 4897 325
Size: 445 1498
Fragments arranged by size:
1498 1255 971 721 499 445 326 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3360 4316 6028
Size: 274 80 33 51 1443 1383 956 1712
Cuts at: 6028 96
Size: 138
Fragments arranged by size:
1712 1443 1383 956 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 2710 3406 2710
Size: 696 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 3395 1870
Size: 1525 4545
XmnI GAAnn'nnTTC
Cuts at: 2253 4199 2253
Size: 1946 4124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI