Restriction map for
pFN21ASDA0860
(Circular) (Six-base) MAPSORT of: pFN21ASDA0860.seq Check: 406 from: 1 to: 6352
pFN21ASDA0860 6352 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3054 3054
Size: 6352
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3695 1937
Size: 1758 4594
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 4415 5799 6067 1632
Size: 504 2279 1384 268 1917
Fragments arranged by size:
2279 1917 1384 504 268
AflII C'TTAA_G
Cuts at: 820 1017 2539 820
Size: 197 1522 4633
Fragments arranged by size:
4633 1522 197
AflIII A'CryG_T
Cuts at: 1203 3133 5251 6228 1203
Size: 1930 2118 977 1327
Fragments arranged by size:
2118 1930 1327 977
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 2558 1166
Size: 1392 4960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AlwNI CAG_nnn'CTG
Cuts at: 2776 2977 3301 4095 5667 2776
Size: 201 324 794 1572 3461
Fragments arranged by size:
3461 1572 794 324 201
ApaI G_GGCC'C
Cuts at: 2333 3464 2333
Size: 1131 5221
ApaLI G'TGCA_C
Cuts at: 3592 4409 5565 3592
Size: 817 1156 4379
Fragments arranged by size:
4379 1156 817
ApoI r'AATT_y
Cuts at: 1493 2061 3661 3887 4039 6148 1493
Size: 568 1600 226 152 2109 1697
Fragments arranged by size:
2109 1697 1600 568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 1870
Size: 73 1734 4545
Fragments arranged by size:
4545 1734 73
BamHI G'GATC_C
Cuts at: 2815 3682 4049 2815
Size: 867 367 5118
Fragments arranged by size:
5118 867 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3428 3673 5128 618
Size: 325 217 627 1641 245 1455 1842
Fragments arranged by size:
1842 1641 1455 627 325 245 217
BanII G_rGCy'C
Cuts at: 1391 2333 2499 2515 3249 3464 3671 1391
Size: 942 166 16 734 215 207 4072
Fragments arranged by size:
4072 942 734 215 207 166 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2854 2888 4543 4577 1903
Size: 34 917 34 1655 34 3678
Fragments arranged by size:
3678 1655 917 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2585 4244 5454 2585
Size: 1659 1210 3483
Fragments arranged by size:
3483 1659 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2424 4963 136
Size: 107 122 71 631 605 752 2539 1525
Fragments arranged by size:
2539 1525 752 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2119 6347 2119
Size: 4228 2124
BlpI GC'TnA_GC
Cuts at: 2337 3769 2337
Size: 1432 4920
Bme1580I G_kGCm'C
Cuts at: 1790 2333 2931 3464 3596 4413 5569 1790
Size: 543 598 533 132 817 1156 2573
Fragments arranged by size:
2573 1156 817 598 543 533 132
BmgBI CAC'GTC
Cuts at: 1318 3591 3628 1318
Size: 2273 37 4042
Fragments arranged by size:
4042 2273 37
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2308 2925 4083
Size: 416 53 83 399 24 891 617 1158
Cuts at: 4083 5041 442
Size: 958 1753
Fragments arranged by size:
1753 1158 958 891 617 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3621 1056
Size: 2565 3787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2682 2746 2849 2909 3005 3368 3439 5012
Size: 1443 64 103 60 96 363 71 1573
Cuts at: 5012 1239
Size: 2579
Fragments arranged by size:
2579 1573 1443 363 103 96 71 64
60
Bpu10I CC'TnA_GC
Cuts at: 2522 2522
Size: 6352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 4477 5342 5640 2978
Size: 1499 865 298 3690
Fragments arranged by size:
3690 1499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5015 882
Size: 617 3516 2219
Fragments arranged by size:
3516 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2530 3649 4143 493
Size: 711 1326 1119 494 2702
Fragments arranged by size:
2702 1326 1119 711 494
BsaBI GATnn'nnATC
Cuts at: 2549 6346 2549
Size: 3797 2555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4159 4541 5176 275
Size: 53 83 186 936 2626 382 635 1451
Fragments arranged by size:
2626 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4785 5183 5457 5604 6015 4785
Size: 398 274 147 411 5122
Fragments arranged by size:
5122 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3503 3533 1178
Size: 30 165 30 2100 30 3997
Fragments arranged by size:
3997 2100 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 1186
Size: 1633 4719
BseYI C'CCAG_C
Cuts at: 2237 2332 3248 5555 6051 2237
Size: 95 916 2307 496 2538
Fragments arranged by size:
2538 2307 916 496 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5779
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3611 3802 4563 5591 1540
Size: 275 186 1610 191 761 1028 2301
Fragments arranged by size:
2301 1610 1028 761 275 191 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2885 3061 3216 3596 4413 4498 5569 2499
Size: 386 176 155 380 817 85 1071 3282
Fragments arranged by size:
3282 1071 817 386 380 176 155 85
BsmI GAATG_Cn'
Cuts at: 3863 3956 3863
Size: 93 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2499 2515 2885 2931 3061 3216
Size: 399 543 166 16 370 46 130 155
Cuts at: 3216 3249 3464 3596 3671 4413 4498 5569 1391
Size: 33 215 132 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 399 370 215 166
155 132 130 85 75 46 33 16
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 4241 5971 4241
Size: 1730 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2630 2679 3054 3707 844
Size: 1786 49 375 653 3489
Fragments arranged by size:
3489 1786 653 375 49
BsrBI CCG'CTC
Cuts at: 1394 1823 4239 1394
Size: 429 2416 3507
Fragments arranged by size:
3507 2416 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4847 5021 65
Size: 1183 3599 174 1396
Fragments arranged by size:
3599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2611 3431 3514 3610 4996 5183 1700
Size: 110 801 820 83 96 1386 187 2869
Fragments arranged by size:
2869 1386 820 801 187 110 96 83
BsrGI T'GTAC_A
Cuts at: 96 2271 96
Size: 2175 4177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 3290 4412 5424 3290
Size: 1122 1012 4218
Fragments arranged by size:
4218 1122 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3600 794
Size: 2806 3546
BstBI TT'CG_AA
Cuts at: 2007 5156 2007
Size: 3149 3203
BstEII G'GTnAC_C
Cuts at: 1184 2656 1184
Size: 1472 4880
BstXI CCAn_nnnn'nTGG
Cuts at: 2827 3050 3392 2827
Size: 223 342 5787
Fragments arranged by size:
5787 342 223
BstYI r'GATC_y
Cuts at: 1972 2119 2544 2815 3682 4049 4434 4451 5903
Size: 147 425 271 867 367 385 17 1452
Cuts at: 5903 5989 6001 6347 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 867 425 385 367 346 271
147 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2676 2802 2860 2676
Size: 126 58 6168
Fragments arranged by size:
6168 126 58
BtgI C'CryG_G
Cuts at: 513 1065 2022 2709 513
Size: 552 957 687 4156
Fragments arranged by size:
4156 957 687 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2468 487
Size: 683 56 1242 4371
Fragments arranged by size:
4371 1242 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2911 3081 3216 3868 4662 4682 2065
Size: 846 170 135 652 794 20 3735
Fragments arranged by size:
3735 846 794 652 170 135 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6317
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2613 2778 3535
Size: 54 1425 50 275 18 783 165 757
Cuts at: 3535 3608 3799 4688 6317 8
Size: 73 191 889 1629 43
Fragments arranged by size:
1629 1425 889 783 757 275 191 165
73 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3608 3799 1537
Size: 275 1796 191 4090
Fragments arranged by size:
4090 1796 275 191
EarI CTCTTCn'nnn_
Cuts at: 1353 4282 1353
Size: 2929 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2497 2497
Size: 6352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2682 2746 2849 2909 3005 3368
Size: 393 504 546 64 103 60 96 363
Cuts at: 3368 3439 4415 5012 5799 6067 1239
Size: 71 976 597 787 268 1524
Fragments arranged by size:
1524 976 787 597 546 504 393 363
268 103 96 71 64 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2675 2675
Size: 6352
EcoO109I rG'GnC_Cy
Cuts at: 2439 3461 2439
Size: 1022 5330
EcoRI G'AATT_C
Cuts at: 2061 3661 4039 2061
Size: 1600 378 4374
Fragments arranged by size:
4374 1600 378
EcoRV GAT'ATC
Cuts at: 2079 2079
Size: 6352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2531 2531
Size: 6352
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 5499 1678
Size: 3821 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4993 5025 5067 5099 1373
Size: 32 135 32 3421 32 42 32 2626
Fragments arranged by size:
3421 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3696 3942 669
Size: 988 281 1758 246 3079
Fragments arranged by size:
3079 1758 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3310 748
Size: 2562 3790
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2574 5466 5650 1305
Size: 1269 2892 184 2007
Fragments arranged by size:
2892 2007 1269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4530
Fragments arranged by size:
4530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2558 4310 4669 4828 518
Size: 648 1392 1752 359 159 2042
Fragments arranged by size:
2042 1752 1392 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2237 2336 3526 3607 3768 4445 5593
Size: 478 153 99 1190 81 161 677 1148
Cuts at: 5593 5838 1606
Size: 245 2120
Fragments arranged by size:
2120 1190 1148 677 478 245 161 153
99 81
NaeI GCC'GGC
Cuts at: 2613 2613
Size: 6352
NcoI C'CATG_G
Cuts at: 513 1065 2022 2709 513
Size: 552 957 687 4156
Fragments arranged by size:
4156 957 687 552
NdeI CA'TA_TG
Cuts at: 387 3261 387
Size: 2874 3478
NgoMIV G'CCGG_C
Cuts at: 2611 2611
Size: 6352
NheI G'CTAG_C
Cuts at: 1052 3617 1052
Size: 2565 3787
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 3137 3710 5255 1132
Size: 97 1908 573 1545 2229
Fragments arranged by size:
2229 1908 1545 573 97
PasI CC'CwG_GG
Cuts at: 1348 2326 2729 1348
Size: 978 403 4971
Fragments arranged by size:
4971 978 403
PciI A'CATG_T
Cuts at: 3133 5251 3133
Size: 2118 4234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6352
PfoI T'CCnGG_A
Cuts at: 2811 3416 2811
Size: 605 5747
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PmlI CAC'GTG
Cuts at: 2530 2530
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 3922 3922
Size: 6352
PspOMI G'GGCC_C
Cuts at: 2329 3460 2329
Size: 1131 5221
PstI C_TGCA'G
Cuts at: 830 2694 2772 3704 830
Size: 1864 78 932 3478
Fragments arranged by size:
3478 1864 932 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
PvuII CAG'CTG
Cuts at: 2084 2237 2336 3526 2084
Size: 153 99 1190 4910
Fragments arranged by size:
4910 1190 153 99
SacI G_AGCT'C
Cuts at: 2499 2499
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SfcI C'TryA_G
Cuts at: 826 1045 2212 2690 2768 3700 4835 5516 5707
Size: 219 1167 478 78 932 1135 681 191
Cuts at: 5707 826
Size: 1471
Fragments arranged by size:
1471 1167 1135 932 681 478 219 191
78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2539 2993 4456 5357 5619 820
Size: 197 926 596 454 1463 901 262 1553
Fragments arranged by size:
1553 1463 926 901 596 454 262 197
SnaBI TAC'GTA
Cuts at: 493 3649 4143 493
Size: 3156 494 2702
Fragments arranged by size:
3156 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3710 1132
Size: 2578 3774
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 3424 1517
Size: 1907 4445
StyI C'CwwG_G
Cuts at: 513 1065 2022 2370 2709 3324 3337 3391 3791
Size: 552 957 348 339 615 13 54 400
Cuts at: 3791 513
Size: 3074
Fragments arranged by size:
3074 957 615 552 400 348 339 54
13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2570 3551 4379 4396 4549 4734 5179
Size: 1255 990 981 828 17 153 185 445
Cuts at: 5179 325
Size: 1498
Fragments arranged by size:
1498 1255 990 981 828 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 4598 6310
Size: 274 80 33 51 1443 294 2327 1712
Cuts at: 6310 96
Size: 138
Fragments arranged by size:
2327 1712 1443 294 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6312 6344 6312
Size: 32 6320
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3187 3187
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 4481 4481
Size: 6352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI
NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI