Restriction map for
pFN21ASDA0859
(Circular) (Six-base) MAPSORT of: pFN21ASDA0859.seq Check: 1386 from: 1 to: 6826
pFN21ASDA0859 6826 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2464 2464
Size: 6826
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4636 278
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3259 4169 1937
Size: 1322 910 4594
Fragments arranged by size:
4594 1322 910
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2543 2666 2995 3332 3864 4889 6273 6541
Size: 911 123 329 337 532 1025 1384 268
Cuts at: 6541 1632
Size: 1917
Fragments arranged by size:
1917 1384 1025 911 532 337 329 268
123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6629
AflIII A'CryG_T
Cuts at: 1203 4077 5725 6702 1203
Size: 2874 1648 977 1327
Fragments arranged by size:
2874 1648 1327 977
AgeI A'CCGG_T
Cuts at: 5657 5657
Size: 6826
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2738 2813 3387 3739 4017 1184
Size: 1554 75 574 352 278 3993
Fragments arranged by size:
3993 1554 574 352 278 75
AlwNI CAG_nnn'CTG
Cuts at: 2669 4569 6141 2669
Size: 1900 1572 3354
Fragments arranged by size:
3354 1900 1572
ApaLI G'TGCA_C
Cuts at: 2570 4883 6039 2570
Size: 2313 1156 3357
Fragments arranged by size:
3357 2313 1156
ApoI r'AATT_y
Cuts at: 1493 4135 4361 4513 6622 1493
Size: 2642 226 152 2109 1697
Fragments arranged by size:
2642 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2547 3400 4151 1870
Size: 73 604 853 751 4545
Fragments arranged by size:
4545 853 751 604 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3241 3274 3241
Size: 33 6793
BamHI G'GATC_C
Cuts at: 4156 4523 4156
Size: 367 6459
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2387 4147 5602 618
Size: 325 217 627 600 1760 1455 1842
Fragments arranged by size:
1842 1760 1455 627 600 325 217
BanII G_rGCy'C
Cuts at: 1391 3912 4017 4145 1391
Size: 2521 105 128 4072
Fragments arranged by size:
4072 2521 128 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2429 928
Size: 971 530 5325
Fragments arranged by size:
5325 971 530
BbvCI CC'TCA_GC
Cuts at: 2454 3272 2454
Size: 818 6008
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5017 5051 1903
Size: 34 3080 34 3678
Fragments arranged by size:
3678 3080 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4718 5928 4718
Size: 1210 5616
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2725 3698 5437
Size: 107 122 71 631 605 1053 973 1739
Cuts at: 5437 136
Size: 1525
Fragments arranged by size:
1739 1525 1053 973 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2685 6821 2685
Size: 4136 2690
BlpI GC'TnA_GC
Cuts at: 3078 4243 3078
Size: 1165 5661
Bme1580I G_kGCm'C
Cuts at: 1790 2390 2574 4887 6043 1790
Size: 600 184 2313 1156 2573
Fragments arranged by size:
2573 2313 1156 600 184
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6826
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3619 3779 4557
Size: 416 53 83 399 24 2202 160 778
Cuts at: 4557 5515 442
Size: 958 1753
Fragments arranged by size:
2202 1753 958 778 416 399 160 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2844 2074
Size: 770 6056
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2750 5486 1239
Size: 1511 2736 2579
Fragments arranged by size:
2736 2579 1511
Bpu10I CC'TnA_GC
Cuts at: 2454 2508 2703 3272 3990 2454
Size: 54 195 569 718 5290
Fragments arranged by size:
5290 718 569 195 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3223 3896 4085 4951 5816 6114 3223
Size: 673 189 866 865 298 3935
Fragments arranged by size:
3935 866 865 673 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2945 5489 882
Size: 617 1446 2544 2219
Fragments arranged by size:
2544 2219 1446 617
BsaAI yAC'GTr
Cuts at: 493 1204 2878 4080 4123 4617 493
Size: 711 1674 1202 43 494 2702
Fragments arranged by size:
2702 1674 1202 711 494 43
BsaBI GATnn'nnATC
Cuts at: 2930 6820 2930
Size: 3890 2936
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4633 5015 5650 275
Size: 53 83 186 936 3100 382 635 1451
Fragments arranged by size:
3100 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3171 5259 5657 5931 6078 6489 3171
Size: 2088 398 274 147 411 3508
Fragments arranged by size:
3508 2088 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2942 2972 3999 4029 1178
Size: 30 165 30 1539 30 1027 30 3975
Fragments arranged by size:
3975 1539 1027 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3720 3723 1186
Size: 2534 3 4289
Fragments arranged by size:
4289 2534 3
BseYI C'CCAG_C
Cuts at: 2815 3109 3113 3126 3790 3905 6029 6525 2815
Size: 294 4 13 664 115 2124 496 3116
Fragments arranged by size:
3116 2124 664 496 294 115 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2780 2809 3311 3968 1163
Size: 573 1044 29 502 657 4021
Fragments arranged by size:
4021 1044 657 573 502 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2485 4276 5037 6065 1540
Size: 275 186 484 1791 761 1028 2301
Fragments arranged by size:
2301 1791 1028 761 484 275 186
BsiHKAI G_wGCw'C
Cuts at: 2574 2704 4017 4887 4972 6043 2574
Size: 130 1313 870 85 1071 3357
Fragments arranged by size:
3357 1313 1071 870 130 85
BsmI GAATG_Cn'
Cuts at: 4337 4430 4337
Size: 93 6733
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2390 2574 2704 3912 4017 4145 4887
Size: 399 600 184 130 1208 105 128 742
Cuts at: 4887 4972 6043 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1208 1071 742 600 399 184 130
128 105 85
BspEI T'CCGG_A
Cuts at: 3171 6489 3171
Size: 3318 3508
BspHI T'CATG_A
Cuts at: 2646 4715 6445 2646
Size: 2069 1730 3027
Fragments arranged by size:
3027 2069 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2464 3124 4181 844
Size: 1620 660 1057 3489
Fragments arranged by size:
3489 1620 1057 660
BsrBI CCG'CTC
Cuts at: 1394 1823 2743 3207 4042 4713 1394
Size: 429 920 464 835 671 3507
Fragments arranged by size:
3507 920 835 671 464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3666 5321 5495 65
Size: 1183 2418 1655 174 1396
Fragments arranged by size:
2418 1655 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5470 5657 1700
Size: 110 3660 187 2869
Fragments arranged by size:
3660 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6826
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 4886 5898 4886
Size: 1012 5814
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6826
BstBI TT'CG_AA
Cuts at: 2007 5630 2007
Size: 3623 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6826
BstXI CCAn_nnnn'nTGG
Cuts at: 3632 3632
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 2685 3498 3966 4156 4523 4908 4925 6377
Size: 713 813 468 190 367 385 17 1452
Cuts at: 6377 6463 6475 6821 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 813 713 468 385 367 346
190 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2940 4049 2940
Size: 1109 5717
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2856 2889 487
Size: 683 56 1630 33 4424
Fragments arranged by size:
4424 1630 683 56 33
BtsI GCAGTG_nn'
Cuts at: 2538 3924 4342 5136 5156 2538
Size: 1386 418 794 20 4208
Fragments arranged by size:
4208 1386 794 418 20
ClaI AT'CG_AT
Cuts at: 1554 3597 1554
Size: 2043 4783
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3492 3527 529
Size: 35 2928 35 3828
Fragments arranged by size:
3828 2928 35 35
DraI TTT'AAA
Cuts at: 4130 4130
Size: 6826
DraIII CAC_nnn'GTG
Cuts at: 2578 2881 3627 2578
Size: 303 746 5777
Fragments arranged by size:
5777 746 303
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3167 4082 5833 809
Size: 507 1851 915 1751 1802
Fragments arranged by size:
1851 1802 1751 915 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2461 2580 2748
Size: 54 1425 50 275 18 631 119 168
Cuts at: 2748 2997 3618 4273 5162 6791 8
Size: 249 621 655 889 1629 43
Fragments arranged by size:
1629 1425 889 655 631 621 275 249
168 119 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4273 1537
Size: 275 2461 4090
Fragments arranged by size:
4090 2461 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2412 4756 1353
Size: 1059 2344 3423
Fragments arranged by size:
3423 2344 1059
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3774 5413 5787 5933 387
Size: 1064 138 2185 1639 374 146 1280
Fragments arranged by size:
2185 1639 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4015 4015
Size: 6826
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 2666 2750 2995 3332 3864 4889
Size: 393 911 123 84 245 337 532 1025
Cuts at: 4889 5486 6273 6541 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1025 911 787 597 532 393 337
268 245 123 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2507 2507
Size: 6826
EcoO109I rG'GnC_Cy
Cuts at: 3136 3385 3760 3136
Size: 249 375 6202
Fragments arranged by size:
6202 375 249
EcoRI G'AATT_C
Cuts at: 4135 4513 4135
Size: 378 6448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3268 3268
Size: 6826
FspI TGC'GCA
Cuts at: 5332 5332
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 5973 1678
Size: 4295 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5467 5499 5541 5573 1373
Size: 32 135 32 3895 32 42 32 2626
Fragments arranged by size:
3895 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2065 3260 3751 4170 4416 669
Size: 988 281 127 1195 491 419 246 3079
Fragments arranged by size:
3079 1195 988 491 419 281 246 127
HindIII A'AGCT_T
Cuts at: 748 2103 748
Size: 1355 5471
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 4425 4425
Size: 6826
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 2583 3591 5940 6124 1305
Size: 1053 225 1008 2349 184 2007
Fragments arranged by size:
2349 2007 1053 1008 225 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 3620 10
Size: 54 1425 343 750 1038 3216
Fragments arranged by size:
3216 1425 1038 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4784 5143 5302 518
Size: 648 3618 359 159 2042
Fragments arranged by size:
3618 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2797 2992 3113 3367 3982 4242 4919 6067
Size: 1191 195 121 254 615 260 677 1148
Cuts at: 6067 6312 1606
Size: 245 2120
Fragments arranged by size:
2120 1191 1148 677 615 260 254 245
195 121
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6274
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6826
NotI GC'GGCC_GC
Cuts at: 4273 4273
Size: 6826
NspI r_CATG'y
Cuts at: 1132 1229 4184 5729 1132
Size: 97 2955 1545 2229
Fragments arranged by size:
2955 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2166 1348
Size: 818 6008
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2602 2669 2881 3627 4036 1108
Size: 1494 67 212 746 409 3898
Fragments arranged by size:
3898 1494 746 409 212 67
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PmlI CAC'GTG
Cuts at: 2878 2878
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2603 2635 3956 3988 4895 4927 6408 6440 2603
Size: 32 1321 32 907 32 1481 32 2989
Fragments arranged by size:
2989 1481 1321 907 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3136 3385 3760 3136
Size: 249 375 6202
Fragments arranged by size:
6202 375 249
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 4396 4396
Size: 6826
PstI C_TGCA'G
Cuts at: 830 2475 3197 3397 4178 830
Size: 1645 722 200 781 3478
Fragments arranged by size:
3478 1645 781 722 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 2797 2992 3113 3982 2797
Size: 195 121 869 5641
Fragments arranged by size:
5641 869 195 121
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 6826
SacI G_AGCT'C
Cuts at: 4017 4017
Size: 6826
SalI G'TCGA_C
Cuts at: 1936 3258 4168 1936
Size: 1322 910 4594
Fragments arranged by size:
4594 1322 910
SbfI CC_TGCA'GG
Cuts at: 2475 4178 2475
Size: 1703 5123
ScaI AGT'ACT
Cuts at: 5074 6786 5074
Size: 1712 5114
SexAI A'CCwGG_T
Cuts at: 2595 2595
Size: 6826
SfcI C'TryA_G
Cuts at: 826 1045 2471 3193 3393 3815 4174 5309 5990
Size: 219 1426 722 200 422 359 1135 681
Cuts at: 5990 6181 826
Size: 191 1471
Fragments arranged by size:
1471 1426 1135 722 681 422 359 219
200 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 2549 3402 4153 1872
Size: 677 853 751 4545
Fragments arranged by size:
4545 853 751 677
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3202 3911 4100 4930 5831 6093
Size: 197 926 1259 709 189 830 901 262
Cuts at: 6093 820
Size: 1553
Fragments arranged by size:
1553 1259 926 901 830 709 262 197
189
SnaBI TAC'GTA
Cuts at: 493 4123 4617 493
Size: 3630 494 2702
Fragments arranged by size:
3630 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6826
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SspI AAT'ATT
Cuts at: 5 52 4750 5
Size: 47 4698 2081
Fragments arranged by size:
4698 2081 47
StuI AGG'CCT
Cuts at: 1517 3525 1517
Size: 2008 4818
StyI C'CwwG_G
Cuts at: 513 1065 4265 513
Size: 552 3200 3074
Fragments arranged by size:
3200 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2178 2969 3140 3143 3638 3666 4853
Size: 1255 598 791 171 3 495 28 1187
Cuts at: 4853 4870 5023 5208 5653 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1187 791 598 495 445 185
171 153 28 17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5072 6784 96
Size: 274 80 33 51 1443 3095 1712 138
Fragments arranged by size:
3095 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 3999 4031 6786 6818 2942
Size: 32 1025 32 2755 32 2950
Fragments arranged by size:
2950 2755 1025 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3215 1316
Size: 1899 4927
XbaI T'CTAG_A
Cuts at: 4162 4162
Size: 6826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3613 3671 3613
Size: 58 6768
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6826
XmaI C'CCGG_G
Cuts at: 1870 2547 3400 4151 1870
Size: 677 853 751 4545
Fragments arranged by size:
4545 853 751 677
XmnI GAAnn'nnTTC
Cuts at: 2081 2105 4955 2081
Size: 24 2850 3952
Fragments arranged by size:
3952 2850 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4634 276
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI
SacII SanDI SapI SfiI SfoI SrfI SwaI