Restriction map for
pFN21ASDA0854
(Circular) (Six-base) MAPSORT of: pFN21ASDA0854.seq Check: 2483 from: 1 to: 7240
pFN21ASDA0854 7240 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5050 278
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 2313 4583 1937
Size: 166 210 2270 4594
Fragments arranged by size:
4594 2270 210 166
Acc65I G'GTAC_C
Cuts at: 4561 4561
Size: 7240
AclI AA'CG_TT
Cuts at: 1563 5367 5740 1563
Size: 3804 373 3063
Fragments arranged by size:
3804 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 5303 6687 6955 1632
Size: 833 2838 1384 268 1917
Fragments arranged by size:
2838 1917 1384 833 268
AfeI AGC'GCT
Cuts at: 2963 2963
Size: 7240
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7043
AflIII A'CryG_T
Cuts at: 1203 2352 2743 6139 7116 1203
Size: 1149 391 3396 977 1327
Fragments arranged by size:
3396 1327 1149 977 391
AgeI A'CCGG_T
Cuts at: 4332 6071 4332
Size: 1739 5501
AhdI GACnn_n'nnGTC
Cuts at: 5969 5969
Size: 7240
AleI CACnn'nnGTG
Cuts at: 1166 3089 1166
Size: 1923 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7240
AlwNI CAG_nnn'CTG
Cuts at: 2946 4523 4983 6555 2946
Size: 1577 460 1572 3631
Fragments arranged by size:
3631 1577 1572 460
ApaLI G'TGCA_C
Cuts at: 5297 6453 5297
Size: 1156 6084
ApoI r'AATT_y
Cuts at: 1493 4549 4775 4927 7036 1493
Size: 3056 226 152 2109 1697
Fragments arranged by size:
3056 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5794 160
Size: 872 4762 1606
Fragments arranged by size:
4762 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2423 2630 2645 4565 1870
Size: 73 150 330 207 15 1920 4545
Fragments arranged by size:
4545 1920 330 207 150 73 15
BamHI G'GATC_C
Cuts at: 4570 4937 4570
Size: 367 6873
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2617 3029 3298 3878 4561
Size: 325 217 627 830 412 269 580 683
Cuts at: 4561 6016 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 830 683 627 580 412 325
269 217
BanII G_rGCy'C
Cuts at: 1391 2724 3309 3841 4181 4559 1391
Size: 1333 585 532 340 378 4072
Fragments arranged by size:
4072 1333 585 532 378 340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3365 3663 928
Size: 971 1466 298 4505
Fragments arranged by size:
4505 1466 971 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3521 3555 5431 5465 1903
Size: 34 1584 34 1876 34 3678
Fragments arranged by size:
3678 1876 1584 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2830 5132 6342 2830
Size: 2302 1210 3728
Fragments arranged by size:
3728 2302 1210
BclI T'GATC_A
Cuts at: 1548 2853 4126 1548
Size: 1305 1273 4662
Fragments arranged by size:
4662 1305 1273
BfrBI ATG'CAT
Cuts at: 2055 2055
Size: 7240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3077 3563 4356
Size: 107 122 71 631 605 1405 486 793
Cuts at: 4356 5851 136
Size: 1495 1525
Fragments arranged by size:
1525 1495 1405 793 631 605 486 122
107 71
BglII A'GATC_T
Cuts at: 7235 7235
Size: 7240
BlpI GC'TnA_GC
Cuts at: 3725 4657 3725
Size: 932 6308
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2622 2790 3883 5301 6457 1790
Size: 304 528 168 1093 1418 1156 2573
Fragments arranged by size:
2573 1418 1156 1093 528 304 168
BmgBI CAC'GTC
Cuts at: 1318 2146 2746 1318
Size: 828 600 5812
Fragments arranged by size:
5812 828 600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3067 3877 3967
Size: 416 53 83 399 24 1650 810 90
Cuts at: 3967 4971 5929 442
Size: 1004 958 1753
Fragments arranged by size:
1753 1650 1004 958 810 416 399 90
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2031 1056
Size: 975 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 3760 4342 2716
Size: 1044 582 5614
Fragments arranged by size:
5614 1044 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3092 3383 4474 5900 1239
Size: 1853 291 1091 1426 2579
Fragments arranged by size:
2579 1853 1426 1091 291
Bpu10I CC'TnA_GC
Cuts at: 3294 3707 3294
Size: 413 6827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2686 3368 5365 6230 6528 2686
Size: 682 1997 865 298 3398
Fragments arranged by size:
3398 1997 865 682 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3235 3710 5903 882
Size: 617 1736 475 2193 2219
Fragments arranged by size:
2219 2193 1736 617 475
BsaAI yAC'GTr
Cuts at: 493 1204 2353 2656 3585 4402 4537 5031 493
Size: 711 1149 303 929 817 135 494 2702
Fragments arranged by size:
2702 1149 929 817 711 494 303 135
BsaBI GATnn'nnATC
Cuts at: 7234 7234
Size: 7240
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5047 5429 6064 275
Size: 53 83 186 936 3514 382 635 1451
Fragments arranged by size:
3514 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3875 4332 5673 6071 6345 6492 6903 3875
Size: 457 1341 398 274 147 411 4212
Fragments arranged by size:
4212 1341 457 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2393 2423 3857 3887 1178
Size: 30 165 30 990 30 1434 30 4531
Fragments arranged by size:
4531 1434 990 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 2714 2945 3017 3967 4394 4436 1186
Size: 1462 66 231 72 950 427 42 3990
Fragments arranged by size:
3990 1462 950 427 231 72 66 42
BseYI C'CCAG_C
Cuts at: 2958 3902 6443 6939 2958
Size: 944 2541 496 3259
Fragments arranged by size:
3259 2541 944 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2379 1163
Size: 573 643 6024
Fragments arranged by size:
6024 643 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4416 4690 5451 6479 1540
Size: 275 186 1579 836 274 761 1028 2301
Fragments arranged by size:
2301 1579 1028 836 761 275 274 186
BsiHKAI G_wGCw'C
Cuts at: 2724 2937 3853 3959 5301 5386 6457 2724
Size: 213 916 106 1342 85 1071 3507
Fragments arranged by size:
3507 1342 1071 916 213 106 85
BsmI GAATG_Cn'
Cuts at: 4751 4844 4751
Size: 93 7147
BsmBI CGTCTCn'nnnn_
Cuts at: 3190 3190
Size: 7240
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2622 2724 2790 2937 3309 3841
Size: 399 304 528 102 66 147 372 532
Cuts at: 3841 3853 3883 3959 4181 4559 5301 5386 6457
Size: 12 30 76 222 378 742 85 1071
Cuts at: 6457 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 532 528 399 378 372
304 222 147 102 85 76 66 30
12
BspEI T'CCGG_A
Cuts at: 6903 6903
Size: 7240
BspHI T'CATG_A
Cuts at: 2580 5129 6859 2580
Size: 2549 1730 2961
Fragments arranged by size:
2961 2549 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4595 844
Size: 3751 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3334 5127 1394
Size: 429 1511 1793 3507
Fragments arranged by size:
3507 1793 1511 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5735 5909 65
Size: 1183 4487 174 1396
Fragments arranged by size:
4487 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3432 4332 5884 6071 1700
Size: 110 1622 900 1552 187 2869
Fragments arranged by size:
2869 1622 1552 900 187 110
BsrGI T'GTAC_A
Cuts at: 96 2758 96
Size: 2662 4578
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7240
BssSI C'ACGA_G
Cuts at: 5300 6312 5300
Size: 1012 6228
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2268 794
Size: 1474 5766
BstBI TT'CG_AA
Cuts at: 2007 6044 2007
Size: 4037 3203
BstEII G'GTnAC_C
Cuts at: 1184 3163 4258 1184
Size: 1979 1095 4166
Fragments arranged by size:
4166 1979 1095
BstYI r'GATC_y
Cuts at: 1972 2556 2694 3129 4570 4937 5322 5339 6791
Size: 584 138 435 1441 367 385 17 1452
Cuts at: 6791 6877 6889 7235 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1441 584 435 385 367 346
138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3950 3950
Size: 7240
BtgI C'CryG_G
Cuts at: 513 1065 2022 3210 3229 3804 4116 4167 513
Size: 552 957 1188 19 575 312 51 3586
Fragments arranged by size:
3586 1188 957 575 552 312 51 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3274 3544 487
Size: 683 56 2048 270 4183
Fragments arranged by size:
4183 2048 683 270 56
BtsI GCAGTG_nn'
Cuts at: 4756 5550 5570 4756
Size: 794 20 6426
Fragments arranged by size:
6426 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7205
DraI TTT'AAA
Cuts at: 4544 4544
Size: 7240
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3172 6247 809
Size: 507 1856 3075 1802
Fragments arranged by size:
3075 1856 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 3136 3232
Size: 54 1425 50 275 18 318 988 96
Cuts at: 3232 3321 3555 4008 4170 4357 4525 4687 5576
Size: 89 234 453 162 187 168 162 889
Cuts at: 5576 7205 8
Size: 1629 43
Fragments arranged by size:
1629 1425 988 889 453 318 275 234
187 168 162 162 96 89 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4687 1537
Size: 275 2875 4090
Fragments arranged by size:
4090 2875 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2188 2988 3233 4233 5170 1353
Size: 835 800 245 1000 937 3423
Fragments arranged by size:
3423 1000 937 835 800 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3778 5827 6201 6347 387
Size: 1064 138 2189 2049 374 146 1280
Fragments arranged by size:
2189 2049 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2722 2722
Size: 7240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 3092 3383 4474 5303 5900 6687
Size: 393 833 627 291 1091 829 597 787
Cuts at: 6687 6955 1239
Size: 268 1524
Fragments arranged by size:
1524 1091 833 829 787 627 597 393
291 268
EcoO109I rG'GnC_Cy
Cuts at: 2794 4219 2794
Size: 1425 5815
EcoRI G'AATT_C
Cuts at: 4549 4927 4549
Size: 378 6862
EcoRV GAT'ATC
Cuts at: 3497 3497
Size: 7240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3262 3409 3262
Size: 147 7093
FspI TGC'GCA
Cuts at: 5746 5746
Size: 7240
HaeII r_GCGC'y
Cuts at: 1678 2965 6387 1678
Size: 1287 3422 2531
Fragments arranged by size:
3422 2531 1287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2381 2393 2413
Size: 32 135 32 626 32 151 12 20
Cuts at: 2413 2425 3364 3396 3857 3889 4418 4450 5881
Size: 12 939 32 461 32 529 32 1431
Cuts at: 5881 5913 5955 5987 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1431 939 626 529 461 151 135
42 32 32 32 32 32 32 32
32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2314 3177 3888 4584 4830 669
Size: 988 281 376 863 711 696 246 3079
Fragments arranged by size:
3079 988 863 711 696 376 281 246
HindIII A'AGCT_T
Cuts at: 748 3767 748
Size: 3019 4221
HpaI GTT'AAC
Cuts at: 3888 4830 3888
Size: 942 6298
KpnI G_GTAC'C
Cuts at: 4565 4565
Size: 7240
MfeI C'AATT_G
Cuts at: 3918 4839 3918
Size: 921 6319
MluI A'CGCG_T
Cuts at: 7116 7116
Size: 7240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2424 4461 4527 6354 6538 1305
Size: 1119 2037 66 1827 184 2007
Fragments arranged by size:
2037 2007 1827 1119 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 3234 3323 3557 4527
Size: 54 1425 343 318 1084 89 234 970
Cuts at: 4527 10
Size: 2723
Fragments arranged by size:
2723 1425 1084 970 343 318 234 89
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3059 3089 3443 5198 5557 5716 518
Size: 648 1893 30 354 1755 359 159 2042
Fragments arranged by size:
2042 1893 1755 648 359 354 159 30
MspA1I CmG'CkG
Cuts at: 1606 2690 2764 2912 2982 3171 4656 5333 6481
Size: 1084 74 148 70 189 1485 677 1148
Cuts at: 6481 6726 1606
Size: 245 2120
Fragments arranged by size:
2120 1485 1148 1084 677 245 189 148
74 70
NcoI C'CATG_G
Cuts at: 513 1065 2022 3229 3804 4116 4167 513
Size: 552 957 1207 575 312 51 3586
Fragments arranged by size:
3586 1207 957 575 552 312 51
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7240
NheI G'CTAG_C
Cuts at: 1052 2027 1052
Size: 975 6265
NotI GC'GGCC_GC
Cuts at: 4687 4687
Size: 7240
NsiI A_TGCA'T
Cuts at: 2057 2057
Size: 7240
NspI r_CATG'y
Cuts at: 1132 1229 2312 2320 4598 6143 1132
Size: 97 1083 8 2278 1545 2229
Fragments arranged by size:
2278 2229 1545 1083 97 8
PacI TTA_AT'TAA
Cuts at: 2454 2454
Size: 7240
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7240
PciI A'CATG_T
Cuts at: 6139 6139
Size: 7240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3832 4117 1108
Size: 2724 285 4231
Fragments arranged by size:
4231 2724 285
PmeI GTTT'AAAC
Cuts at: 4544 4544
Size: 7240
PmlI CAC'GTG
Cuts at: 2656 2656
Size: 7240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5309 5341 6822 6854 5309
Size: 32 1481 32 5695
Fragments arranged by size:
5695 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2794 4219 2794
Size: 1425 5815
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7240
PsiI TTA'TAA
Cuts at: 4810 4810
Size: 7240
PstI C_TGCA'G
Cuts at: 830 4519 4592 830
Size: 3689 73 3478
Fragments arranged by size:
3689 3478 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7240
PvuII CAG'CTG
Cuts at: 2690 2764 2912 2982 2690
Size: 74 148 70 6948
Fragments arranged by size:
6948 148 74 70
SacI G_AGCT'C
Cuts at: 2724 2724
Size: 7240
SalI G'TCGA_C
Cuts at: 1936 2312 4582 1936
Size: 376 2270 4594
Fragments arranged by size:
4594 2270 376
SbfI CC_TGCA'GG
Cuts at: 4592 4592
Size: 7240
ScaI AGT'ACT
Cuts at: 5488 7200 5488
Size: 1712 5528
SfcI C'TryA_G
Cuts at: 826 1045 4515 4588 5723 6404 6595 826
Size: 219 3470 73 1135 681 191 1471
Fragments arranged by size:
3470 1471 1135 681 219 191 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 3563 3563
Size: 7240
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7240
SmaI CCC'GGG
Cuts at: 1872 2425 4567 1872
Size: 553 2142 4545
Fragments arranged by size:
4545 2142 553
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2701 3383 5344 6245 6507 820
Size: 197 926 758 682 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 758 682 262 197
SnaBI TAC'GTA
Cuts at: 493 4537 5031 493
Size: 4044 494 2702
Fragments arranged by size:
4044 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7240
SphI G_CATG'C
Cuts at: 1132 4598 1132
Size: 3466 3774
SspI AAT'ATT
Cuts at: 5 52 4211 5164 5
Size: 47 4159 953 2081
Fragments arranged by size:
4159 2081 953 47
StuI AGG'CCT
Cuts at: 1517 4532 1517
Size: 3015 4225
StyI C'CwwG_G
Cuts at: 513 1065 2022 2151 2245 2790 3229 3246 3315
Size: 552 957 129 94 545 439 17 69
Cuts at: 3315 3804 4116 4167 4679 513
Size: 489 312 51 512 3074
Fragments arranged by size:
3074 957 552 545 512 489 439 312
129 94 69 51 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2940 2972 3328 5267 5284 5437 5622
Size: 1255 1360 32 356 1939 17 153 185
Cuts at: 5622 6067 325
Size: 445 1498
Fragments arranged by size:
1939 1498 1360 1255 445 356 185 153
32 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2758 5486 7198
Size: 274 80 33 51 1443 781 2728 1712
Cuts at: 7198 96
Size: 138
Fragments arranged by size:
2728 1712 1443 781 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7200 7232 7200
Size: 32 7208
Tth111I GACn'n_nGTC
Cuts at: 1316 3576 4508 1316
Size: 2260 932 4048
Fragments arranged by size:
4048 2260 932
XbaI T'CTAG_A
Cuts at: 4576 4576
Size: 7240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3550 4018 3550
Size: 468 6772
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7240
XmaI C'CCGG_G
Cuts at: 1870 2423 4565 1870
Size: 553 2142 4545
Fragments arranged by size:
4545 2142 553
XmnI GAAnn'nnTTC
Cuts at: 2297 3020 3479 3939 5369 2297
Size: 723 459 460 1430 4168
Fragments arranged by size:
4168 1430 723 460 459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5048 276
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI PfoI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI