Restriction map for
pFN21ASDA0849
(Circular) (Six-base) MAPSORT of: pFN21ASDA0849.seq Check: 8642 from: 1 to: 7588
pFN21ASDA0849 7588 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5398 278
Size: 53 83 186 936 3862 2468
Fragments arranged by size:
3862 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4931 1937
Size: 2994 4594
Acc65I G'GTAC_C
Cuts at: 4909 4909
Size: 7588
AclI AA'CG_TT
Cuts at: 1563 5715 6088 1563
Size: 4152 373 3063
Fragments arranged by size:
4152 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2449 3462 3550 3617 3641 4065 5651 7035
Size: 817 1013 88 67 24 424 1586 1384
Cuts at: 7035 7303 1632
Size: 268 1917
Fragments arranged by size:
1917 1586 1384 1013 817 424 268 88
67 24
AfeI AGC'GCT
Cuts at: 3683 3683
Size: 7588
AflII C'TTAA_G
Cuts at: 820 1017 3712 820
Size: 197 2695 4696
Fragments arranged by size:
4696 2695 197
AflIII A'CryG_T
Cuts at: 1203 6487 7464 1203
Size: 5284 977 1327
Fragments arranged by size:
5284 1327 977
AgeI A'CCGG_T
Cuts at: 6419 6419
Size: 7588
AhdI GACnn_n'nnGTC
Cuts at: 6317 6317
Size: 7588
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2274 4861 1184
Size: 1090 2587 3911
Fragments arranged by size:
3911 2587 1090
AlwNI CAG_nnn'CTG
Cuts at: 2678 3050 5331 6903 2678
Size: 372 2281 1572 3363
Fragments arranged by size:
3363 2281 1572 372
ApaLI G'TGCA_C
Cuts at: 4815 5645 6801 4815
Size: 830 1156 5602
Fragments arranged by size:
5602 1156 830
ApoI r'AATT_y
Cuts at: 1493 2241 2444 4064 4161 4244 4317 4897 5123
Size: 748 203 1620 97 83 73 580 226
Cuts at: 5123 5275 7384 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1620 748 580 226 203 152
97 83 73
AseI AT'TA_AT
Cuts at: 160 1032 6142 160
Size: 872 5110 1606
Fragments arranged by size:
5110 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7588
AvaI C'yCGr_G
Cuts at: 1870 1943 4913 1870
Size: 73 2970 4545
Fragments arranged by size:
4545 2970 73
BamHI G'GATC_C
Cuts at: 4918 5285 4918
Size: 367 7221
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4255 4909 6364 618
Size: 325 217 627 2468 654 1455 1842
Fragments arranged by size:
2468 1842 1455 654 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3434 4907 1391
Size: 2043 1473 4072
Fragments arranged by size:
4072 2043 1473
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3087 3165 4365 4782 4828 928
Size: 971 1188 78 1200 417 46 3688
Fragments arranged by size:
3688 1200 1188 971 417 78 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5779 5813 1903
Size: 34 3842 34 3678
Fragments arranged by size:
3842 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5480 6690 5480
Size: 1210 6378
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7588
BfrBI ATG'CAT
Cuts at: 4838 4838
Size: 7588
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6199 136
Size: 107 122 71 631 605 4527 1525
Fragments arranged by size:
4527 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2429 3065 7583 2429
Size: 636 4518 2434
Fragments arranged by size:
4518 2434 636
BlpI GC'TnA_GC
Cuts at: 5005 5005
Size: 7588
Bme1580I G_kGCm'C
Cuts at: 1790 3595 4819 5649 6805 1790
Size: 1805 1224 830 1156 2573
Fragments arranged by size:
2573 1805 1224 1156 830
BmgBI CAC'GTC
Cuts at: 1318 2401 1318
Size: 1083 6505
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 2857 3334
Size: 416 53 83 399 24 660 780 477
Cuts at: 3334 4517 4561 5319 6277 442
Size: 1183 44 758 958 1753
Fragments arranged by size:
1753 1183 958 780 758 660 477 416
399 83 53 44 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2472 2707 3203 4579 4780 6248 1239
Size: 1233 235 496 1376 201 1468 2579
Fragments arranged by size:
2579 1468 1376 1233 496 235 201
Bpu10I CC'TnA_GC
Cuts at: 2803 2803
Size: 7588
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4464 4710 5713 6578 6876 4464
Size: 246 1003 865 298 5176
Fragments arranged by size:
5176 1003 865 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6251 882
Size: 617 4752 2219
Fragments arranged by size:
4752 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4885 5379 493
Size: 711 3681 494 2702
Fragments arranged by size:
3681 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7582 7582
Size: 7588
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5395 5777 6412 275
Size: 53 83 186 936 3862 382 635 1451
Fragments arranged by size:
3862 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2498 4429 6021 6419 6693 6840 7251 2498
Size: 1931 1592 398 274 147 411 2835
Fragments arranged by size:
2835 1931 1592 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2118 2148 3201 3231 1178
Size: 30 165 30 715 30 1053 30 5535
Fragments arranged by size:
5535 1053 715 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 3216 1186
Size: 1171 859 5558
Fragments arranged by size:
5558 1171 859
BseYI C'CCAG_C
Cuts at: 6791 7287 6791
Size: 496 7092
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2891 3351 3564 1163
Size: 573 1155 460 213 5187
Fragments arranged by size:
5187 1155 573 460 213
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4693 5038 5799 6827 1540
Size: 275 186 2692 345 761 1028 2301
Fragments arranged by size:
2692 2301 1028 761 345 275 186
BsiHKAI G_wGCw'C
Cuts at: 3516 4819 5649 5734 6805 3516
Size: 1303 830 85 1071 4299
Fragments arranged by size:
4299 1303 1071 830 85
BsmI GAATG_Cn'
Cuts at: 2108 2191 4421 4783 5099 5192 2108
Size: 83 2230 362 316 93 4504
Fragments arranged by size:
4504 2230 362 316 93 83
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3434 3516 3595 4819 4907 5649 5734
Size: 399 1644 82 79 1224 88 742 85
Cuts at: 5734 6805 1391
Size: 1071 2174
Fragments arranged by size:
2174 1644 1224 1071 742 399 88 85
82 79
BspEI T'CCGG_A
Cuts at: 2498 4429 7251 2498
Size: 1931 2822 2835
Fragments arranged by size:
2835 2822 1931
BspHI T'CATG_A
Cuts at: 5477 7207 5477
Size: 1730 5858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 2683 4118 4478 4788 4943 844
Size: 1819 20 1435 360 310 155 3489
Fragments arranged by size:
3489 1819 1435 360 310 155 20
BsrBI CCG'CTC
Cuts at: 1394 1823 2077 5475 1394
Size: 429 254 3398 3507
Fragments arranged by size:
3507 3398 429 254
BsrDI GCAATG_nn'
Cuts at: 65 1248 3653 4404 6083 6257 65
Size: 1183 2405 751 1679 174 1396
Fragments arranged by size:
2405 1679 1396 1183 751 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3661 6232 6419 1700
Size: 110 1851 2571 187 2869
Fragments arranged by size:
2869 2571 1851 187 110
BsrGI T'GTAC_A
Cuts at: 96 4875 96
Size: 4779 2809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7588
BssSI C'ACGA_G
Cuts at: 5648 6660 5648
Size: 1012 6576
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4578 794
Size: 3784 3804
BstBI TT'CG_AA
Cuts at: 2007 6392 2007
Size: 4385 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7588
BstYI r'GATC_y
Cuts at: 1972 2429 3035 3065 4054 4918 5285 5670 5687
Size: 457 606 30 989 864 367 385 17
Cuts at: 5687 7139 7225 7237 7583 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 989 864 606 457 385 367
346 86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 4059 4059
Size: 7588
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7036
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6849
Fragments arranged by size:
6849 683 56
BtsI GCAGTG_nn'
Cuts at: 2649 3363 5104 5898 5918 2649
Size: 714 1741 794 20 4319
Fragments arranged by size:
4319 1741 794 714 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7588
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7553
DraI TTT'AAA
Cuts at: 2362 4303 4312 4892 2362
Size: 1941 9 580 5058
Fragments arranged by size:
5058 1941 580 9
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6595 809
Size: 507 5279 1802
Fragments arranged by size:
5279 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3313 3393 4686
Size: 54 1425 50 275 18 1483 80 1293
Cuts at: 4686 5035 5924 7553 8
Size: 349 889 1629 43
Fragments arranged by size:
1629 1483 1425 1293 889 349 275 80
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5035 1537
Size: 275 3223 4090
Fragments arranged by size:
4090 3223 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 3348 3617 4493 4676 5518 1353
Size: 714 1281 269 876 183 842 3423
Fragments arranged by size:
3423 1281 876 842 714 269 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6175 6549 6695 387
Size: 1064 138 4586 374 146 1280
Fragments arranged by size:
4586 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2449 2472 2707 3203 3462 3550 3617
Size: 393 817 23 235 496 259 88 67
Cuts at: 3617 3641 4065 4579 4780 5651 6248 7035 7303
Size: 24 424 514 201 871 597 787 268
Cuts at: 7303 1239
Size: 1524
Fragments arranged by size:
1524 871 817 787 597 514 496 424
393 268 259 235 201 88 67 24
23
EcoNI CCTnn'n_nnAGG
Cuts at: 3599 3599
Size: 7588
EcoO109I rG'GnC_Cy
Cuts at: 2471 2675 2471
Size: 204 7384
EcoRI G'AATT_C
Cuts at: 4064 4897 5275 4064
Size: 833 378 6377
Fragments arranged by size:
6377 833 378
EcoRV GAT'ATC
Cuts at: 2926 3186 2926
Size: 260 7328
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2220 4505 2220
Size: 2285 5303
FspI TGC'GCA
Cuts at: 6094 6094
Size: 7588
HaeII r_GCGC'y
Cuts at: 1678 3608 3685 6735 1678
Size: 1930 77 3050 2531
Fragments arranged by size:
3050 2531 1930 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4013 4045 4244 4276 4492
Size: 32 135 32 2441 32 199 32 216
Cuts at: 4492 4524 6229 6261 6303 6335 1373
Size: 32 1705 32 42 32 2626
Fragments arranged by size:
2626 2441 1705 216 199 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3103 4932 5178 669
Size: 988 281 1165 1829 246 3079
Fragments arranged by size:
3079 1829 1165 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2800 748
Size: 2052 5536
HpaI GTT'AAC
Cuts at: 5178 5178
Size: 7588
KpnI G_GTAC'C
Cuts at: 4913 4913
Size: 7588
MfeI C'AATT_G
Cuts at: 2335 5187 2335
Size: 2852 4736
MluI A'CGCG_T
Cuts at: 7464 7464
Size: 7588
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2722 3461 4886 6702 6886 1305
Size: 1417 739 1425 1816 184 2007
Fragments arranged by size:
2007 1816 1425 1417 739 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3315 3395 10
Size: 54 1425 343 1483 80 4203
Fragments arranged by size:
4203 1483 1425 343 80 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2919 3287 4261 4843 5546 5905 6064
Size: 648 1753 368 974 582 703 359 159
Cuts at: 6064 518
Size: 2042
Fragments arranged by size:
2042 1753 974 703 648 582 368 359
159
MspA1I CmG'CkG
Cuts at: 1606 2425 4442 5004 5681 6829 7074 1606
Size: 819 2017 562 677 1148 245 2120
Fragments arranged by size:
2120 2017 1148 819 677 562 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7036
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7588
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7588
NotI GC'GGCC_GC
Cuts at: 5035 5035
Size: 7588
NsiI A_TGCA'T
Cuts at: 4840 4840
Size: 7588
NspI r_CATG'y
Cuts at: 1132 1229 4852 4946 6491 1132
Size: 97 3623 94 1545 2229
Fragments arranged by size:
3623 2229 1545 97 94
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7588
PciI A'CATG_T
Cuts at: 6487 6487
Size: 7588
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4588 1108
Size: 3480 4108
PfoI T'CCnGG_A
Cuts at: 2449 4796 2449
Size: 2347 5241
PmeI GTTT'AAAC
Cuts at: 4892 4892
Size: 7588
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2333 2365 5657 5689 7170 7202 2333
Size: 32 3292 32 1481 32 2719
Fragments arranged by size:
3292 2719 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2471 2675 2471
Size: 204 7384
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7588
PsiI TTA'TAA
Cuts at: 5158 5158
Size: 7588
PstI C_TGCA'G
Cuts at: 830 3632 4940 830
Size: 2802 1308 3478
Fragments arranged by size:
3478 2802 1308
PvuI CG_AT'CG
Cuts at: 2001 4693 2001
Size: 2692 4896
PvuII CAG'CTG
Cuts at: 2425 4442 2425
Size: 2017 5571
SalI G'TCGA_C
Cuts at: 1936 4930 1936
Size: 2994 4594
SapI GCTCTTCn'nnn_
Cuts at: 2067 3348 3617 4676 2067
Size: 1281 269 1059 4979
Fragments arranged by size:
4979 1281 1059 269
SbfI CC_TGCA'GG
Cuts at: 4940 4940
Size: 7588
ScaI AGT'ACT
Cuts at: 4757 5836 7548 4757
Size: 1079 1712 4797
Fragments arranged by size:
4797 1712 1079
SfcI C'TryA_G
Cuts at: 826 1045 3030 3189 3628 4936 6071 6752 6943
Size: 219 1985 159 439 1308 1135 681 191
Cuts at: 6943 826
Size: 1471
Fragments arranged by size:
1985 1471 1308 1135 681 439 219 191
159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7588
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7588
SmaI CCC'GGG
Cuts at: 1872 4915 1872
Size: 3043 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4479 4725 5692 6593 6855
Size: 197 926 1769 767 246 967 901 262
Cuts at: 6855 820
Size: 1553
Fragments arranged by size:
1769 1553 967 926 901 767 262 246
197
SnaBI TAC'GTA
Cuts at: 493 4885 5379 493
Size: 4392 494 2702
Fragments arranged by size:
4392 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7588
SphI G_CATG'C
Cuts at: 1132 4946 1132
Size: 3814 3774
SspI AAT'ATT
Cuts at: 5 52 2505 3908 4075 5512 5
Size: 47 2453 1403 167 1437 2081
Fragments arranged by size:
2453 2081 1437 1403 167 47
StuI AGG'CCT
Cuts at: 1517 2388 2959 3634 1517
Size: 871 571 675 5471
Fragments arranged by size:
5471 871 675 571
StyI C'CwwG_G
Cuts at: 513 1065 2566 3390 4788 4807 5027 513
Size: 552 1501 824 1398 19 220 3074
Fragments arranged by size:
3074 1501 1398 824 552 220 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 5615 5632 5785 5970 6415 325
Size: 1255 1896 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2419 2900 4755
Size: 274 80 33 51 1443 442 481 1855
Cuts at: 4755 4875 5834 7546 96
Size: 120 959 1712 138
Fragments arranged by size:
1855 1712 1443 959 481 442 274 138
120 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2118 2150 3201 3233 3524 3556 7548 7580 2118
Size: 32 1051 32 291 32 3992 32 2126
Fragments arranged by size:
3992 2126 1051 291 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7588
XbaI T'CTAG_A
Cuts at: 2245 3421 4924 2245
Size: 1176 1503 4909
Fragments arranged by size:
4909 1503 1176
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7588
XmaI C'CCGG_G
Cuts at: 1870 4913 1870
Size: 3043 4545
XmnI GAAnn'nnTTC
Cuts at: 2445 3257 4712 5717 2445
Size: 812 1455 1005 4316
Fragments arranged by size:
4316 1455 1005 812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5396 276
Size: 53 83 186 936 3862 2468
Fragments arranged by size:
3862 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI