Restriction map for
pFN21ASDA0848
(Circular) (Six-base) MAPSORT of: pFN21ASDA0848.seq Check: 899 from: 1 to: 7660
pFN21ASDA0848 7660 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5470 278
Size: 53 83 186 936 3934 2468
Fragments arranged by size:
3934 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4192 5003 1937
Size: 2255 811 4594
Fragments arranged by size:
4594 2255 811
Acc65I G'GTAC_C
Cuts at: 4981 4981
Size: 7660
AclI AA'CG_TT
Cuts at: 1563 5787 6160 1563
Size: 4224 373 3063
Fragments arranged by size:
4224 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 2771 3505 4069 5723 7107 7375 1632
Size: 793 346 734 564 1654 1384 268 1917
Fragments arranged by size:
1917 1654 1384 793 734 564 346 268
AflII C'TTAA_G
Cuts at: 820 1017 2393 820
Size: 197 1376 6087
Fragments arranged by size:
6087 1376 197
AflIII A'CryG_T
Cuts at: 1203 2732 6559 7536 1203
Size: 1529 3827 977 1327
Fragments arranged by size:
3827 1529 1327 977
AgeI A'CCGG_T
Cuts at: 6491 6491
Size: 7660
AhdI GACnn_n'nnGTC
Cuts at: 6389 6389
Size: 7660
AleI CACnn'nnGTG
Cuts at: 1166 4511 1166
Size: 3345 4315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4145 1184
Size: 2961 4699
AlwNI CAG_nnn'CTG
Cuts at: 5403 6975 5403
Size: 1572 6088
ApaI G_GGCC'C
Cuts at: 3971 4480 3971
Size: 509 7151
ApaLI G'TGCA_C
Cuts at: 4766 5717 6873 4766
Size: 951 1156 5553
Fragments arranged by size:
5553 1156 951
ApoI r'AATT_y
Cuts at: 1493 2281 2904 3310 4969 5195 5347 7456 1493
Size: 788 623 406 1659 226 152 2109 1697
Fragments arranged by size:
2109 1697 1659 788 623 406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2340 6214 160
Size: 872 1308 3874 1606
Fragments arranged by size:
3874 1606 1308 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7660
AvaI C'yCGr_G
Cuts at: 1870 1943 4883 4985 1870
Size: 73 2940 102 4545
Fragments arranged by size:
4545 2940 102 73
AvrII C'CTAG_G
Cuts at: 4396 4396
Size: 7660
BamHI G'GATC_C
Cuts at: 4990 5357 4990
Size: 367 7293
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3925 4981 6436 618
Size: 325 217 627 2138 1056 1455 1842
Fragments arranged by size:
2138 1842 1455 1056 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2151 2703 3803 3971 4480 4979 1391
Size: 760 552 1100 168 509 499 4072
Fragments arranged by size:
4072 1100 760 552 509 499 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2431 2858 4943 928
Size: 971 532 427 2085 3645
Fragments arranged by size:
3645 2085 971 532 427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5851 5885 1903
Size: 34 3914 34 3678
Fragments arranged by size:
3914 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5552 6762 5552
Size: 1210 6450
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7660
BfrBI ATG'CAT
Cuts at: 2357 2936 2357
Size: 579 7081
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6271 136
Size: 107 122 71 631 605 4599 1525
Fragments arranged by size:
4599 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7655 7655
Size: 7660
BlpI GC'TnA_GC
Cuts at: 5077 5077
Size: 7660
Bme1580I G_kGCm'C
Cuts at: 1790 3928 3971 4480 4746 4770 5721 6877 1790
Size: 2138 43 509 266 24 951 1156 2573
Fragments arranged by size:
2573 2138 1156 951 509 266 43 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7660
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4590 5391
Size: 416 53 83 399 24 1739 1434 801
Cuts at: 5391 6349 442
Size: 958 1753
Fragments arranged by size:
1753 1739 1434 958 801 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2397 2687 3903 3924 4194 4952 6320 1239
Size: 1158 290 1216 21 270 758 1368 2579
Fragments arranged by size:
2579 1368 1216 1158 758 290 270 21
Bpu10I CC'TnA_GC
Cuts at: 3021 3021
Size: 7660
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5785 6650 6948 5785
Size: 865 298 6497
Fragments arranged by size:
6497 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2801 6323 882
Size: 617 1302 3522 2219
Fragments arranged by size:
3522 2219 1302 617
BsaAI yAC'GTr
Cuts at: 493 1204 2633 4054 4765 4957 5451 493
Size: 711 1429 1421 711 192 494 2702
Fragments arranged by size:
2702 1429 1421 711 711 494 192
BsaBI GATnn'nnATC
Cuts at: 7654 7654
Size: 7660
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3588 4066 5467 5849
Size: 53 83 186 936 2055 478 1401 382
Cuts at: 5849 6484 275
Size: 635 1451
Fragments arranged by size:
2055 1451 1401 936 635 478 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4446 6093 6491 6765 6912 7323 4446
Size: 1647 398 274 147 411 4783
Fragments arranged by size:
4783 1647 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7435
Fragments arranged by size:
7435 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3948 4316 4319 4322 1186
Size: 2762 368 3 3 4524
Fragments arranged by size:
4524 2762 368 3 3
BseYI C'CCAG_C
Cuts at: 3892 3897 4497 4543 6863 7359 3892
Size: 5 600 46 2320 496 4193
Fragments arranged by size:
4193 2320 600 496 46 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3703 3851 4365 4690 4745 1163
Size: 573 1967 148 514 325 55 4078
Fragments arranged by size:
4078 1967 573 514 325 148 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5110 5871 6899 1540
Size: 275 186 3109 761 1028 2301
Fragments arranged by size:
3109 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 4059 4770 5721 5806 6877 2703
Size: 1356 711 951 85 1071 3486
Fragments arranged by size:
3486 1356 1071 951 711 85
BsmI GAATG_Cn'
Cuts at: 2673 2769 2938 3253 3682 5171 5264 2673
Size: 96 169 315 429 1489 93 5069
Fragments arranged by size:
5069 1489 429 315 169 96 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2703 3803 3928 3971 4059 4480
Size: 399 361 552 1100 125 43 88 421
Cuts at: 4480 4746 4770 4979 5721 5806 6877 1391
Size: 266 24 209 742 85 1071 2174
Fragments arranged by size:
2174 1100 1071 742 552 421 399 361
266 209 125 88 85 43 24
BspEI T'CCGG_A
Cuts at: 7323 7323
Size: 7660
BspHI T'CATG_A
Cuts at: 5549 7279 5549
Size: 1730 5930
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5015 844
Size: 4171 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5547 1394
Size: 429 3724 3507
Fragments arranged by size:
3724 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2357 3099 4139 6155 6329 65
Size: 1183 1109 742 1040 2016 174 1396
Fragments arranged by size:
2016 1396 1183 1109 1040 742 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6304 6491 1700
Size: 110 4494 187 2869
Fragments arranged by size:
4494 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2729 2743 96
Size: 2633 14 5013
Fragments arranged by size:
5013 2633 14
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7660
BssSI C'ACGA_G
Cuts at: 4296 5720 6732 4296
Size: 1424 1012 5224
Fragments arranged by size:
5224 1424 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4343 794
Size: 3549 4111
BstBI TT'CG_AA
Cuts at: 2007 6464 2007
Size: 4457 3203
BstEII G'GTnAC_C
Cuts at: 1184 4261 1184
Size: 3077 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 2825 2825
Size: 7660
BstYI r'GATC_y
Cuts at: 1972 3325 4990 5357 5742 5759 7211 7297 7309
Size: 1353 1665 367 385 17 1452 86 12
Cuts at: 7309 7655 1972
Size: 346 1977
Fragments arranged by size:
1977 1665 1452 1353 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3623 3623
Size: 7660
BtgI C'CryG_G
Cuts at: 513 1065 2824 3651 4227 513
Size: 552 1759 827 576 3946
Fragments arranged by size:
3946 1759 827 576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6921
Fragments arranged by size:
6921 683 56
BtsI GCAGTG_nn'
Cuts at: 4491 5176 5970 5990 4491
Size: 685 794 20 6161
Fragments arranged by size:
6161 794 685 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7660
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7625
DraI TTT'AAA
Cuts at: 2263 2403 2562 3742 4964 2263
Size: 140 159 1180 1222 4959
Fragments arranged by size:
4959 1222 1180 159 140
DraIII CAC_nnn'GTG
Cuts at: 3150 3150
Size: 7660
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6667 809
Size: 507 5351 1802
Fragments arranged by size:
5351 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2939 5107 5996
Size: 54 1425 50 275 18 1109 2168 889
Cuts at: 5996 7625 8
Size: 1629 43
Fragments arranged by size:
2168 1629 1425 1109 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5107 1537
Size: 275 3295 4090
Fragments arranged by size:
4090 3295 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2747 3410 4835 5590 1353
Size: 1394 663 1425 755 3423
Fragments arranged by size:
3423 1425 1394 755 663
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4188 4592 6247 6621 6767 387
Size: 1064 138 2599 404 1655 374 146 1280
Fragments arranged by size:
2599 1655 1280 1064 404 374 146 138
EcoICRI GAG'CTC
Cuts at: 2701 2701
Size: 7660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2397 2425 2687 2771 3505 3903 3924
Size: 393 765 28 262 84 734 398 21
Cuts at: 3924 4069 4194 4952 5723 6320 7107 7375 1239
Size: 145 125 758 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 765 758 734 597 398
393 268 262 145 125 84 28 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 4647 2123
Size: 2524 5136
EcoO109I rG'GnC_Cy
Cuts at: 2833 3240 3372 3967 3968 4219 4477 2833
Size: 407 132 595 1 251 258 6016
Fragments arranged by size:
6016 595 407 258 251 132 1
EcoRI G'AATT_C
Cuts at: 2281 2904 4969 5347 2281
Size: 623 2065 378 4594
Fragments arranged by size:
4594 2065 623 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3421 3652 4099 4841 3421
Size: 231 447 742 6240
Fragments arranged by size:
6240 742 447 231
FspI TGC'GCA
Cuts at: 3830 6166 3830
Size: 2336 5324
FspAI rTGC'GCAy
Cuts at: 3830 3830
Size: 7660
HaeII r_GCGC'y
Cuts at: 1678 6807 1678
Size: 5129 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2398 2430 3111
Size: 32 135 32 662 32 132 32 681
Cuts at: 3111 3143 3259 3291 4507 4539 6301 6333 6375
Size: 32 116 32 1216 32 1762 32 42
Cuts at: 6375 6407 1373
Size: 32 2626
Fragments arranged by size:
2626 1762 1216 681 662 135 132 116
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3193 4193 5004 5250 669
Size: 988 281 1255 1000 811 246 3079
Fragments arranged by size:
3079 1255 1000 988 811 281 246
HindIII A'AGCT_T
Cuts at: 748 3069 748
Size: 2321 5339
HpaI GTT'AAC
Cuts at: 5250 5250
Size: 7660
KpnI G_GTAC'C
Cuts at: 4985 4985
Size: 7660
MfeI C'AATT_G
Cuts at: 2088 5259 2088
Size: 3171 4489
MluI A'CGCG_T
Cuts at: 7536 7536
Size: 7660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2612 6774 6958 1305
Size: 1307 4162 184 2007
Fragments arranged by size:
4162 2007 1307 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2941 10
Size: 54 1425 343 1109 4729
Fragments arranged by size:
4729 1425 1109 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2579 2823 3171 3675 3822 4127 4239
Size: 648 1413 244 348 504 147 305 112
Cuts at: 4239 4511 5618 5977 6136 518
Size: 272 1107 359 159 2042
Fragments arranged by size:
2042 1413 1107 648 504 359 348 305
272 244 159 147 112
MspA1I CmG'CkG
Cuts at: 1606 2707 3881 5076 5753 6901 7146 1606
Size: 1101 1174 1195 677 1148 245 2120
Fragments arranged by size:
2120 1195 1174 1148 1101 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2824 513
Size: 552 1759 5349
Fragments arranged by size:
5349 1759 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7660
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7660
NotI GC'GGCC_GC
Cuts at: 5107 5107
Size: 7660
NsiI A_TGCA'T
Cuts at: 2359 2938 2359
Size: 579 7081
NspI r_CATG'y
Cuts at: 1132 1229 2736 3448 4783 5018 6563 1132
Size: 97 1507 712 1335 235 1545 2229
Fragments arranged by size:
2229 1545 1507 1335 712 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7660
PciI A'CATG_T
Cuts at: 2732 6559 2732
Size: 3827 3833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4550 1108
Size: 3442 4218
PfoI T'CCnGG_A
Cuts at: 3498 3663 4929 3498
Size: 165 1266 6229
Fragments arranged by size:
6229 1266 165
PmeI GTTT'AAAC
Cuts at: 4964 4964
Size: 7660
PmlI CAC'GTG
Cuts at: 4765 4765
Size: 7660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5729 5761 7242 7274 5729
Size: 32 1481 32 6115
Fragments arranged by size:
6115 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2833 2833
Size: 7660
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7660
PsiI TTA'TAA
Cuts at: 5230 5230
Size: 7660
PspOMI G'GGCC_C
Cuts at: 3967 4476 3967
Size: 509 7151
PspXI vC'TCGA_Gb
Cuts at: 4883 4883
Size: 7660
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 7628
PstI C_TGCA'G
Cuts at: 830 2277 2487 3517 4039 5012 830
Size: 1447 210 1030 522 973 3478
Fragments arranged by size:
3478 1447 1030 973 522 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7660
PvuII CAG'CTG
Cuts at: 2707 3881 2707
Size: 1174 6486
SacI G_AGCT'C
Cuts at: 2703 2703
Size: 7660
SalI G'TCGA_C
Cuts at: 1936 4191 5002 1936
Size: 2255 811 4594
Fragments arranged by size:
4594 2255 811
SbfI CC_TGCA'GG
Cuts at: 5012 5012
Size: 7660
ScaI AGT'ACT
Cuts at: 5908 7620 5908
Size: 1712 5948
SexAI A'CCwGG_T
Cuts at: 3729 3729
Size: 7660
SfcI C'TryA_G
Cuts at: 826 1045 2273 2483 3458 3513 4035 5008 6143
Size: 219 1228 210 975 55 522 973 1135
Cuts at: 6143 6824 7015 826
Size: 681 191 1471
Fragments arranged by size:
1471 1228 1135 975 973 681 522 219
210 191 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7660
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7660
SmaI CCC'GGG
Cuts at: 1872 4987 1872
Size: 3115 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 4883 5764 6665 6927 820
Size: 197 926 450 2490 881 901 262 1553
Fragments arranged by size:
2490 1553 926 901 881 450 262 197
SnaBI TAC'GTA
Cuts at: 493 4957 5451 493
Size: 4464 494 2702
Fragments arranged by size:
4464 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7660
SphI G_CATG'C
Cuts at: 1132 4783 5018 1132
Size: 3651 235 3774
Fragments arranged by size:
3774 3651 235
SspI AAT'ATT
Cuts at: 5 52 2227 5584 5
Size: 47 2175 3357 2081
Fragments arranged by size:
3357 2175 2081 47
StuI AGG'CCT
Cuts at: 1517 3456 4041 1517
Size: 1939 585 5136
Fragments arranged by size:
5136 1939 585
StyI C'CwwG_G
Cuts at: 513 1065 2458 2720 2824 3047 3178 3236 3321
Size: 552 1393 262 104 223 131 58 85
Cuts at: 3321 3710 4396 4425 5099 513
Size: 389 686 29 674 3074
Fragments arranged by size:
3074 1393 686 674 552 389 262 223
131 104 85 58 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3138 4373 4438 4557 5687 5704 5857
Size: 1255 1558 1235 65 119 1130 17 153
Cuts at: 5857 6042 6487 325
Size: 185 445 1498
Fragments arranged by size:
1558 1498 1255 1235 1130 445 185 153
119 65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2729 2743 3477
Size: 274 80 33 51 1443 752 14 734
Cuts at: 3477 4245 5906 7618 96
Size: 768 1661 1712 138
Fragments arranged by size:
1712 1661 1443 768 752 734 274 138
80 51 33 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7620 7652 7620
Size: 32 7628
Tth111I GACn'n_nGTC
Cuts at: 1316 3731 1316
Size: 2415 5245
XbaI T'CTAG_A
Cuts at: 2473 4996 2473
Size: 2523 5137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4547 4616 4547
Size: 69 7591
XhoI C'TCGA_G
Cuts at: 1943 4883 1943
Size: 2940 4720
XmaI C'CCGG_G
Cuts at: 1870 4985 1870
Size: 3115 4545
XmnI GAAnn'nnTTC
Cuts at: 2769 3231 3635 4824 5789 2769
Size: 462 404 1189 965 4640
Fragments arranged by size:
4640 1189 965 462 404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5468 276
Size: 53 83 186 936 3934 2468
Fragments arranged by size:
3934 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I EcoRV FseI KasI NaeI NarI
NgoMIV NruI PacI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI