Restriction map for
pFN21ASDA0841
(Circular) (Six-base) MAPSORT of: pFN21ASDA0841.seq Check: 5009 from: 1 to: 6628
pFN21ASDA0841 6628 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3031 3031
Size: 6628
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3971 1937
Size: 2034 4594
Acc65I G'GTAC_C
Cuts at: 3949 3949
Size: 6628
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2978 3755 4691 6075 6343 1632
Size: 1346 777 936 1384 268 1917
Fragments arranged by size:
1917 1384 1346 936 777 268
AfeI AGC'GCT
Cuts at: 3507 3507
Size: 6628
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 3016 5527 6504 1203
Size: 1813 2511 977 1327
Fragments arranged by size:
2511 1813 1327 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 5357 5357
Size: 6628
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2790 3425 1184
Size: 1606 635 4387
Fragments arranged by size:
4387 1606 635
AlwNI CAG_nnn'CTG
Cuts at: 2314 2470 2617 2704 2881 2995 3046 3115 3145
Size: 156 147 87 177 114 51 69 30
Cuts at: 3145 3676 3733 3766 3856 3913 4371 5943 2314
Size: 531 57 33 90 57 458 1572 2999
Fragments arranged by size:
2999 1572 531 458 177 156 147 114
90 87 69 57 57 51 33 30
ApaI G_GGCC'C
Cuts at: 2101 2416 2611 3904 2101
Size: 315 195 1293 4825
Fragments arranged by size:
4825 1293 315 195
ApaLI G'TGCA_C
Cuts at: 2171 4685 5841 2171
Size: 2514 1156 2958
Fragments arranged by size:
2958 2514 1156
ApoI r'AATT_y
Cuts at: 1493 3937 4163 4315 6424 1493
Size: 2444 226 152 2109 1697
Fragments arranged by size:
2444 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 2094 3274 3953 1870
Size: 73 151 1180 679 4545
Fragments arranged by size:
4545 1180 679 151 73
BamHI G'GATC_C
Cuts at: 2534 3026 3177 3958 4325 2534
Size: 492 151 781 367 4837
Fragments arranged by size:
4837 781 492 367 151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2080 2487 3189 3331 3949
Size: 325 217 627 293 407 702 142 618
Cuts at: 3949 5404 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 702 627 618 407 325 293
217 142
BanII G_rGCy'C
Cuts at: 1391 2101 2401 2416 2568 2611 2711 3087 3275
Size: 710 300 15 152 43 100 376 188
Cuts at: 3275 3494 3846 3904 3947 1391
Size: 219 352 58 43 4072
Fragments arranged by size:
4072 710 376 352 300 219 188 152
100 58 43 43 15
BbeI G_GCGC'C
Cuts at: 2491 3193 2491
Size: 702 5926
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2440 3647 928
Size: 971 541 1207 3909
Fragments arranged by size:
3909 1207 971 541
BbvCI CC'TCA_GC
Cuts at: 3149 3149
Size: 6628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2577 2611 4819 4853 1903
Size: 34 640 34 2208 34 3678
Fragments arranged by size:
3678 2208 640 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4520 5730 4520
Size: 1210 5418
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6628
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2991 3871 3899
Size: 107 122 71 631 605 1319 880 28
Cuts at: 3899 5239 136
Size: 1340 1525
Fragments arranged by size:
1525 1340 1319 880 631 605 122 107
71 28
BglII A'GATC_T
Cuts at: 6623 6623
Size: 6628
BlpI GC'TnA_GC
Cuts at: 2705 3509 4045 2705
Size: 804 536 5288
Fragments arranged by size:
5288 804 536
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2101 2175 2416 2611 3404 3904 4689
Size: 295 16 74 241 195 793 500 785
Cuts at: 4689 5845 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 793 785 500 295 241 195
74 16
BmgBI CAC'GTC
Cuts at: 1318 2369 2544 2749 1318
Size: 1051 175 205 5197
Fragments arranged by size:
5197 1051 205 175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 2605 3062
Size: 416 53 83 399 24 643 545 457
Cuts at: 3062 3862 4359 5317 442
Size: 800 497 958 1753
Fragments arranged by size:
1753 958 800 643 545 497 457 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2033 3290 1056
Size: 977 1257 4394
Fragments arranged by size:
4394 1257 977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2420 2420
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2582 2854 2972 3383 3478 3516 3764
Size: 1097 246 272 118 411 95 38 248
Cuts at: 3764 3788 5288 1239
Size: 24 1500 2579
Fragments arranged by size:
2579 1500 1097 411 272 248 246 118
95 38 24
Bpu10I CC'TnA_GC
Cuts at: 2343 3149 3780 2343
Size: 806 631 5191
Fragments arranged by size:
5191 806 631
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2364 3071 4753 5618 5916 2364
Size: 707 1682 865 298 3076
Fragments arranged by size:
3076 1682 865 707 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2941 3200 5291 882
Size: 617 1442 259 2091 2219
Fragments arranged by size:
2219 2091 1442 617 259
BsaAI yAC'GTr
Cuts at: 493 1204 3925 4419 493
Size: 711 2721 494 2702
Fragments arranged by size:
2721 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6622 6622
Size: 6628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2488 3190 4435 4817
Size: 53 83 186 936 955 702 1245 382
Cuts at: 4817 5452 275
Size: 635 1451
Fragments arranged by size:
1451 1245 955 936 702 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6403
Fragments arranged by size:
6403 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2396 3017 3218 3380 3854 1186
Size: 1207 3 621 201 162 474 3960
Fragments arranged by size:
3960 1207 621 474 201 162 3
BseYI C'CCAG_C
Cuts at: 2058 2388 2420 2940 2979 3128 3520 3621 3882
Size: 330 32 520 39 149 392 101 261
Cuts at: 3882 5831 6327 2058
Size: 1949 496 2359
Fragments arranged by size:
2359 1949 520 496 392 330 261 149
101 39 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2581 2827 3080 3167 3455 3937 1163
Size: 573 845 246 253 87 288 482 3854
Fragments arranged by size:
3854 845 573 482 288 253 246 87
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 4078 4839 5867 1540
Size: 275 186 66 2011 761 1028 2301
Fragments arranged by size:
2301 2011 1028 761 275 186 66
BsiHKAI G_wGCw'C
Cuts at: 2175 2401 2711 2769 2951 3087 3846 4689 4774
Size: 226 310 58 182 136 759 843 85
Cuts at: 4774 5845 2175
Size: 1071 2958
Fragments arranged by size:
2958 1071 843 759 310 226 182 136
85 58
BsmI GAATG_Cn'
Cuts at: 4139 4232 4139
Size: 93 6535
BsmBI CGTCTCn'nnnn_
Cuts at: 2711 3007 2711
Size: 296 6332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2085 2101 2175 2401 2416 2568 2611
Size: 399 295 16 74 226 15 152 43
Cuts at: 2611 2711 2769 2951 3087 3275 3404 3494 3846
Size: 100 58 182 136 188 129 90 352
Cuts at: 3846 3904 3947 4689 4774 5845 1391
Size: 58 43 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 295 226 188
182 152 136 129 100 90 85 74
58 58 43 43 16 15
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 2668 4517 6247 2668
Size: 1849 1730 3049
Fragments arranged by size:
3049 1849 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3031 3983 844
Size: 2187 952 3489
Fragments arranged by size:
3489 2187 952
BsrBI CCG'CTC
Cuts at: 1394 1823 2782 2965 4515 1394
Size: 429 959 183 1550 3507
Fragments arranged by size:
3507 1550 959 429 183
BsrDI GCAATG_nn'
Cuts at: 65 1248 5123 5297 65
Size: 1183 3875 174 1396
Fragments arranged by size:
3875 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3559 5272 5459 1700
Size: 110 1749 1713 187 2869
Fragments arranged by size:
2869 1749 1713 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 4688 5700 4688
Size: 1012 5616
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 2470 3145 794
Size: 1376 300 675 4277
Fragments arranged by size:
4277 1376 675 300
BstBI TT'CG_AA
Cuts at: 2007 5432 2007
Size: 3425 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6628
BstYI r'GATC_y
Cuts at: 1972 2196 2455 2534 3026 3177 3625 3684 3958
Size: 224 259 79 492 151 448 59 274
Cuts at: 3958 4325 4710 4727 6179 6265 6277 6623 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 492 448 385 367 346 274
259 224 151 86 79 59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2085 2355 2568 2889 3444 513
Size: 552 957 63 270 213 321 555 3697
Fragments arranged by size:
3697 957 555 552 321 270 213 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5889
Fragments arranged by size:
5889 683 56
BtsI GCAGTG_nn'
Cuts at: 2705 2815 3333 4144 4938 4958 2705
Size: 110 518 811 794 20 4375
Fragments arranged by size:
4375 811 794 518 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6593
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2884 5635 809
Size: 507 1568 2751 1802
Fragments arranged by size:
2751 1802 1568 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2221 2352 3246
Size: 54 1425 50 275 18 391 131 894
Cuts at: 3246 4075 4964 6593 8
Size: 829 889 1629 43
Fragments arranged by size:
1629 1425 894 889 829 391 275 131
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4075 1537
Size: 275 2263 4090
Fragments arranged by size:
4090 2263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 3579 3731 4558 1353
Size: 711 1515 152 827 3423
Fragments arranged by size:
3423 1515 827 711 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2932 3212 5215 5589 5735 387
Size: 1064 138 1343 280 2003 374 146 1280
Fragments arranged by size:
2003 1343 1280 1064 374 280 146 138
EcoICRI GAG'CTC
Cuts at: 2399 2709 3085 3844 2399
Size: 310 376 759 5183
Fragments arranged by size:
5183 759 376 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2582 2854 2972 2978 3383 3478
Size: 393 704 246 272 118 6 405 95
Cuts at: 3478 3516 3755 3764 3788 4691 5288 6075 6343
Size: 38 239 9 24 903 597 787 268
Cuts at: 6343 1239
Size: 1524
Fragments arranged by size:
1524 903 787 704 597 405 393 272
268 246 239 118 95 38 24 9
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2957 2999 3725 3836 2957
Size: 42 726 111 5749
Fragments arranged by size:
5749 726 111 42
EcoO109I rG'GnC_Cy
Cuts at: 2098 2851 2992 3091 3583 3853 3900 3901 2098
Size: 753 141 99 492 270 47 1 4825
Fragments arranged by size:
4825 753 492 270 141 99 47 1
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
EcoRV GAT'ATC
Cuts at: 2838 2838
Size: 6628
FspI TGC'GCA
Cuts at: 2117 5134 2117
Size: 3017 3611
HaeII r_GCGC'y
Cuts at: 1678 2491 2986 3067 3193 3509 5775 1678
Size: 813 495 81 126 316 2266 2531
Fragments arranged by size:
2531 2266 813 495 316 126 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2783 2815 5269 5301 5343
Size: 32 135 32 1211 32 2454 32 42
Cuts at: 5343 5375 1373
Size: 32 2626
Fragments arranged by size:
2626 2454 1211 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3972 4218 669
Size: 988 281 2034 246 3079
Fragments arranged by size:
3079 2034 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6628
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KasI G'GCGC_C
Cuts at: 2487 3189 2487
Size: 702 5926
KpnI G_GTAC'C
Cuts at: 3953 3953
Size: 6628
MfeI C'AATT_G
Cuts at: 4227 4227
Size: 6628
MluI A'CGCG_T
Cuts at: 3016 6504 3016
Size: 3488 3140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2228 5742 5926 1305
Size: 923 3514 184 2007
Fragments arranged by size:
3514 2007 923 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2223 10
Size: 54 1425 343 391 4415
Fragments arranged by size:
4415 1425 391 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 4586 4945 5104 518
Size: 648 1188 2232 359 159 2042
Fragments arranged by size:
2232 2042 1188 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2467 2944 3128 3175 3196 3469 3607 4044
Size: 861 477 184 47 21 273 138 437
Cuts at: 4044 4721 5869 6114 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 861 677 477 437 273 245
184 138 47 21
NaeI GCC'GGC
Cuts at: 3561 3561
Size: 6628
NarI GG'CG_CC
Cuts at: 2488 3190 2488
Size: 702 5926
NcoI C'CATG_G
Cuts at: 513 1065 2022 2355 2889 513
Size: 552 957 333 534 4252
Fragments arranged by size:
4252 957 552 534 333
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6628
NgoMIV G'CCGG_C
Cuts at: 3559 3559
Size: 6628
NheI G'CTAG_C
Cuts at: 1052 2029 3286 1052
Size: 977 1257 4394
Fragments arranged by size:
4394 1257 977
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 2171 3986 5531 1132
Size: 97 942 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 942 97
PasI CC'CwG_GG
Cuts at: 1348 3895 1348
Size: 2547 4081
PciI A'CATG_T
Cuts at: 5527 5527
Size: 6628
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 2752 2995 3199 3520 1108
Size: 1206 438 243 204 321 4216
Fragments arranged by size:
4216 1206 438 321 243 204
PfoI T'CCnGG_A
Cuts at: 2295 2904 2295
Size: 609 6019
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 6210 6242 4697
Size: 32 1481 32 5083
Fragments arranged by size:
5083 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3583 3583
Size: 6628
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6628
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PspOMI G'GGCC_C
Cuts at: 2097 2412 2607 3900 2097
Size: 315 195 1293 4825
Fragments arranged by size:
4825 1293 315 195
PstI C_TGCA'G
Cuts at: 830 2475 2496 2622 2779 3120 3600 3684 3789
Size: 1645 21 126 157 341 480 84 105
Cuts at: 3789 3909 3980 830
Size: 120 71 3478
Fragments arranged by size:
3478 1645 480 341 157 126 120 105
84 71 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
PvuII CAG'CTG
Cuts at: 3128 3196 3469 3128
Size: 68 273 6287
Fragments arranged by size:
6287 273 68
SacI G_AGCT'C
Cuts at: 2401 2711 3087 3846 2401
Size: 310 376 759 5183
Fragments arranged by size:
5183 759 376 310
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SbfI CC_TGCA'GG
Cuts at: 2496 3600 3980 2496
Size: 1104 380 5144
Fragments arranged by size:
5144 1104 380
ScaI AGT'ACT
Cuts at: 4876 6588 4876
Size: 1712 4916
SfcI C'TryA_G
Cuts at: 826 1045 2471 2492 2618 2775 3116 3158 3596
Size: 219 1426 21 126 157 341 42 438
Cuts at: 3596 3680 3785 3905 3976 5111 5792 5983 826
Size: 84 105 120 71 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 681 438 341 219 191
157 126 120 105 84 71 42 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 3899 3899
Size: 6628
SfoI GGC'GCC
Cuts at: 2489 3191 2489
Size: 702 5926
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 2096 3276 3955 1872
Size: 224 1180 679 4545
Fragments arranged by size:
4545 1180 679 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2379 3086 4732 5633 5895 820
Size: 197 926 436 707 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 707 436 262 197
SnaBI TAC'GTA
Cuts at: 493 3925 4419 493
Size: 3432 494 2702
Fragments arranged by size:
3432 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SrfI GCCC'GGGC
Cuts at: 2096 2096
Size: 6628
SspI AAT'ATT
Cuts at: 5 52 4552 5
Size: 47 4500 2081
Fragments arranged by size:
4500 2081 47
StuI AGG'CCT
Cuts at: 1517 3542 3660 1517
Size: 2025 118 4485
Fragments arranged by size:
4485 2025 118
StyI C'CwwG_G
Cuts at: 513 1065 2022 2355 2760 2889 3485 4067 513
Size: 552 957 333 405 129 596 582 3074
Fragments arranged by size:
3074 957 596 582 552 405 333 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 2787 2915 3683 4655 4672 4825
Size: 1255 822 385 128 768 972 17 153
Cuts at: 4825 5010 5455 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 972 822 768 445 385 185
153 128 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4874 6586 96
Size: 274 80 33 51 1443 2897 1712 138
Fragments arranged by size:
2897 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2911 2943 3464 3496 6588 6620 2911
Size: 32 521 32 3092 32 2919
Fragments arranged by size:
3092 2919 521 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6628
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 2094 3274 3953 1870
Size: 224 1180 679 4545
Fragments arranged by size:
4545 1180 679 224
XmnI GAAnn'nnTTC
Cuts at: 4757 4757
Size: 6628
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
Bsu36I DraIII FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SwaI XcmI