Restriction map for
pFN21ASDA0827
(Circular) (Six-base) MAPSORT of: pFN21ASDA0827.seq Check: 5780 from: 1 to: 9322
pFN21ASDA0827 9322 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7132 278
Size: 53 83 186 936 5596 2468
Fragments arranged by size:
5596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4850 6665 1937
Size: 2913 1815 4594
Fragments arranged by size:
4594 2913 1815
Acc65I G'GTAC_C
Cuts at: 2611 2907 5132 6643 2611
Size: 296 2225 1511 5290
Fragments arranged by size:
5290 2225 1511 296
AclI AA'CG_TT
Cuts at: 1563 7449 7822 1563
Size: 5886 373 3063
Fragments arranged by size:
5886 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 2554 2888 4319 4434 4683 4767 4784
Size: 582 340 334 1431 115 249 84 17
Cuts at: 4784 5394 5456 5516 6014 7385 8769 9037 1632
Size: 610 62 60 498 1371 1384 268 1917
Fragments arranged by size:
1917 1431 1384 1371 610 582 498 340
334 268 249 115 84 62 60 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9125
AflIII A'CryG_T
Cuts at: 1203 4644 8221 9198 1203
Size: 3441 3577 977 1327
Fragments arranged by size:
3577 3441 1327 977
AgeI A'CCGG_T
Cuts at: 8153 8153
Size: 9322
AhdI GACnn_n'nnGTC
Cuts at: 5428 8051 5428
Size: 2623 6699
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2345 3733 4518 1184
Size: 1161 1388 785 5988
Fragments arranged by size:
5988 1388 1161 785
AlwNI CAG_nnn'CTG
Cuts at: 3497 3665 4100 4354 4907 7065 8637 3497
Size: 168 435 254 553 2158 1572 4182
Fragments arranged by size:
4182 2158 1572 553 435 254 168
ApaLI G'TGCA_C
Cuts at: 7379 8535 7379
Size: 1156 8166
ApoI r'AATT_y
Cuts at: 1493 4530 5197 6243 6325 6514 6631 6857 7009
Size: 3037 667 1046 82 189 117 226 152
Cuts at: 7009 9118 1493
Size: 2109 1697
Fragments arranged by size:
3037 2109 1697 1046 667 226 189 152
117 82
AseI AT'TA_AT
Cuts at: 160 1032 7876 160
Size: 872 6844 1606
Fragments arranged by size:
6844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9322
AvaI C'yCGr_G
Cuts at: 1870 1943 6647 1870
Size: 73 4704 4545
Fragments arranged by size:
4704 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2595 2628 4191 4224 6095 6128 2595
Size: 33 1563 33 1871 33 5789
Fragments arranged by size:
5789 1871 1563 33 33 33
BamHI G'GATC_C
Cuts at: 6652 7019 6652
Size: 367 8955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2611 2907 5132 6643 8098
Size: 325 217 627 824 296 2225 1511 1455
Cuts at: 8098 618
Size: 1842
Fragments arranged by size:
2225 1842 1511 1455 824 627 325 296
217
BanII G_rGCy'C
Cuts at: 1391 2822 2905 5408 5672 6641 1391
Size: 1431 83 2503 264 969 4072
Fragments arranged by size:
4072 2503 1431 969 264 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5205 928
Size: 971 3306 5045
Fragments arranged by size:
5045 3306 971
BbvCI CC'TCA_GC
Cuts at: 2350 2350
Size: 9322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2885 2919 7513 7547 1903
Size: 34 948 34 4594 34 3678
Fragments arranged by size:
4594 3678 948 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7214 8424 7214
Size: 1210 8112
BclI T'GATC_A
Cuts at: 1548 6424 1548
Size: 4876 4446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7933 136
Size: 107 122 71 631 605 6261 1525
Fragments arranged by size:
6261 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3617 3872 9317 3617
Size: 255 5445 3622
Fragments arranged by size:
5445 3622 255
BlpI GC'TnA_GC
Cuts at: 2048 6739 2048
Size: 4691 4631
Bme1580I G_kGCm'C
Cuts at: 1790 7383 8539 1790
Size: 5593 1156 2573
Fragments arranged by size:
5593 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6043 7053 8011
Size: 416 53 83 399 24 4626 1010 958
Cuts at: 8011 442
Size: 1753
Fragments arranged by size:
4626 1753 1010 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2362 5772 2209
Size: 153 3410 5759
Fragments arranged by size:
5759 3410 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 2826 3633 4452 6138 7982 1239
Size: 948 639 807 819 1686 1844 2579
Fragments arranged by size:
2579 1844 1686 948 819 807 639
Bpu10I CC'TnA_GC
Cuts at: 2350 2350
Size: 9322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5064 5271 5403 6108 7447 8312 8610 5064
Size: 207 132 705 1339 865 298 5776
Fragments arranged by size:
5776 1339 865 705 298 207 132
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4034 7985 882
Size: 617 2535 3951 2219
Fragments arranged by size:
3951 2535 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6619 7113 493
Size: 711 5415 494 2702
Fragments arranged by size:
5415 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2521 9316 2521
Size: 6795 2527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5861 7129 7511 8146
Size: 53 83 186 936 4328 1268 382 635
Cuts at: 8146 275
Size: 1451
Fragments arranged by size:
4328 1451 1268 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 7755 8153 8427 8574 8985 7755
Size: 398 274 147 411 8092
Fragments arranged by size:
8092 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 3631 3661 1178
Size: 30 165 30 711 30 1487 30 6839
Fragments arranged by size:
6839 1487 711 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2190 2230 2338 4884 5335 6078 6201 1186
Size: 1004 40 108 2546 451 743 123 4307
Fragments arranged by size:
4307 2546 1004 743 451 123 108 40
BseYI C'CCAG_C
Cuts at: 3655 8525 9021 3655
Size: 4870 496 3956
Fragments arranged by size:
4870 3956 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4524 1163
Size: 573 2788 5961
Fragments arranged by size:
5961 2788 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6772 7533 8561 1540
Size: 275 186 4771 761 1028 2301
Fragments arranged by size:
4771 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2585 2905 7383 7468 8539 2585
Size: 320 4478 85 1071 3368
Fragments arranged by size:
4478 3368 1071 320 85
BsmI GAATG_Cn'
Cuts at: 3202 6372 6833 6926 3202
Size: 3170 461 93 5598
Fragments arranged by size:
5598 3170 461 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5868 5868
Size: 9322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2585 2822 2905 5408 5672 6641 7383
Size: 399 795 237 83 2503 264 969 742
Cuts at: 7383 7468 8539 1391
Size: 85 1071 2174
Fragments arranged by size:
2503 2174 1071 969 795 742 399 264
237 85 83
BspEI T'CCGG_A
Cuts at: 8985 8985
Size: 9322
BspHI T'CATG_A
Cuts at: 3279 3556 3622 7211 8941 3279
Size: 277 66 3589 1730 3660
Fragments arranged by size:
3660 3589 1730 277 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4930 6677 844
Size: 4086 1747 3489
Fragments arranged by size:
4086 3489 1747
BsrBI CCG'CTC
Cuts at: 1394 1823 7209 1394
Size: 429 5386 3507
Fragments arranged by size:
5386 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2042 3750 4713 5388 6416 6578 7817
Size: 1183 794 1708 963 675 1028 162 1239
Cuts at: 7817 7991 65
Size: 174 1396
Fragments arranged by size:
1708 1396 1239 1183 1028 963 794 675
174 162
BsrFI r'CCGG_y
Cuts at: 1700 1810 7966 8153 1700
Size: 110 6156 187 2869
Fragments arranged by size:
6156 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2448 2878 96
Size: 2352 430 6540
Fragments arranged by size:
6540 2352 430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9322
BssSI C'ACGA_G
Cuts at: 3249 7382 8394 3249
Size: 4133 1012 4177
Fragments arranged by size:
4177 4133 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3668 4100 5504 794
Size: 2874 432 1404 4612
Fragments arranged by size:
4612 2874 1404 432
BstBI TT'CG_AA
Cuts at: 2007 8126 2007
Size: 6119 3203
BstEII G'GTnAC_C
Cuts at: 1184 6111 1184
Size: 4927 4395
BstYI r'GATC_y
Cuts at: 1972 3617 3872 6652 7019 7404 7421 8873 8959
Size: 1645 255 2780 367 385 17 1452 86
Cuts at: 8959 8971 9317 1972
Size: 12 346 1977
Fragments arranged by size:
2780 1977 1645 1452 385 367 346 255
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2466 5655 2466
Size: 3189 6133
BtgI C'CryG_G
Cuts at: 513 1065 2250 2355 2745 2926 5043 5847 513
Size: 552 1185 105 390 181 2117 804 3988
Fragments arranged by size:
3988 2117 1185 804 552 390 181 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4140 487
Size: 683 56 2914 5669
Fragments arranged by size:
5669 2914 683 56
BtsI GCAGTG_nn'
Cuts at: 3305 4402 4880 6838 7632 7652 3305
Size: 1097 478 1958 794 20 4975
Fragments arranged by size:
4975 1958 1097 794 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9287
DraI TTT'AAA
Cuts at: 6626 6626
Size: 9322
DraIII CAC_nnn'GTG
Cuts at: 2728 2728
Size: 9322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3898 8329 809
Size: 507 2582 4431 1802
Fragments arranged by size:
4431 2582 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6435 6769 7658
Size: 54 1425 50 275 18 4605 334 889
Cuts at: 7658 9287 8
Size: 1629 43
Fragments arranged by size:
4605 1629 1425 889 334 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6769 1537
Size: 275 4957 4090
Fragments arranged by size:
4957 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2248 2287 2426 2810 3325 3723 4606 5413
Size: 895 39 139 384 515 398 883 807
Cuts at: 5413 6149 7252 1353
Size: 736 1103 3423
Fragments arranged by size:
3423 1103 895 883 807 736 515 398
384 139 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7909 8283 8429 387
Size: 1064 138 6320 374 146 1280
Fragments arranged by size:
6320 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2903 2903
Size: 9322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2214 2554 2826 2888 3633 4319
Size: 393 555 27 340 272 62 745 686
Cuts at: 4319 4434 4452 4683 4767 4784 5394 5456 5516
Size: 115 18 231 84 17 610 62 60
Cuts at: 5516 6014 6138 7385 7982 8769 9037 1239
Size: 498 124 1247 597 787 268 1524
Fragments arranged by size:
1524 1247 787 745 686 610 597 555
498 393 340 272 268 231 124 115
84 62 62 60 27 18 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2915 5276 2915
Size: 2361 6961
EcoO109I rG'GnC_Cy
Cuts at: 6041 6041
Size: 9322
EcoRI G'AATT_C
Cuts at: 6243 6325 6514 6631 7009 6243
Size: 82 189 117 378 8556
Fragments arranged by size:
8556 378 189 117 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3152 3391 4514 5105 3152
Size: 239 1123 591 7369
Fragments arranged by size:
7369 1123 591 239
FspI TGC'GCA
Cuts at: 7828 7828
Size: 9322
HaeII r_GCGC'y
Cuts at: 1678 8469 1678
Size: 6791 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2054 2056 2085 2086
Size: 32 135 32 452 30 2 29 1
Cuts at: 2086 2117 2546 2578 3261 3293 3464 3496 5165
Size: 31 429 32 683 32 171 32 1669
Cuts at: 5165 5197 5414 5446 7963 7995 8037 8069 1373
Size: 32 217 32 2517 32 42 32 2626
Fragments arranged by size:
2626 2517 1669 683 452 429 217 171
135 42 32 32 32 32 32 32
32 32 32 31 30 29 2 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2394 4408 5086 5433 5538 6666
Size: 988 281 456 2014 678 347 105 1128
Cuts at: 6666 6912 669
Size: 246 3079
Fragments arranged by size:
3079 2014 1128 988 678 456 347 281
246 105
HindIII A'AGCT_T
Cuts at: 748 3373 748
Size: 2625 6697
HpaI GTT'AAC
Cuts at: 2394 5086 6912 2394
Size: 2692 1826 4804
Fragments arranged by size:
4804 2692 1826
KpnI G_GTAC'C
Cuts at: 2615 2911 5136 6647 2615
Size: 296 2225 1511 5290
Fragments arranged by size:
5290 2225 1511 296
MfeI C'AATT_G
Cuts at: 6921 6921
Size: 9322
MluI A'CGCG_T
Cuts at: 9198 9198
Size: 9322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2468 2589 3886 4441 5593 6033 8436 8620
Size: 1163 121 1297 555 1152 440 2403 184
Cuts at: 8620 1305
Size: 2007
Fragments arranged by size:
2403 2007 1297 1163 1152 555 440 184
121
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6437 10
Size: 54 1425 343 4605 2895
Fragments arranged by size:
4605 2895 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4961 5393 7280 7639 7798 518
Size: 648 3795 432 1887 359 159 2042
Fragments arranged by size:
3795 2042 1887 648 432 359 159
MspA1I CmG'CkG
Cuts at: 1606 2207 3665 4114 4354 4745 6738 7415 8563
Size: 601 1458 449 240 391 1993 677 1148
Cuts at: 8563 8808 1606
Size: 245 2120
Fragments arranged by size:
2120 1993 1458 1148 677 601 449 391
245 240
NcoI C'CATG_G
Cuts at: 513 1065 2250 5043 5847 513
Size: 552 1185 2793 804 3988
Fragments arranged by size:
3988 2793 1185 804 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9322
NotI GC'GGCC_GC
Cuts at: 6769 6769
Size: 9322
NruI TCG'CGA
Cuts at: 2092 2092
Size: 9322
NspI r_CATG'y
Cuts at: 1132 1229 6238 6680 8225 1132
Size: 97 5009 442 1545 2229
Fragments arranged by size:
5009 2229 1545 442 97
PasI CC'CwG_GG
Cuts at: 1348 5665 1348
Size: 4317 5005
PciI A'CATG_T
Cuts at: 8221 8221
Size: 9322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9322
PmeI GTTT'AAAC
Cuts at: 6626 6626
Size: 9322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2604 7391 7423 8904 8936 2572
Size: 32 4787 32 1481 32 2958
Fragments arranged by size:
4787 2958 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6041 6041
Size: 9322
PshAI GACnn'nnGTC
Cuts at: 1613 5045 1613
Size: 3432 5890
PsiI TTA'TAA
Cuts at: 6892 6892
Size: 9322
PstI C_TGCA'G
Cuts at: 830 2540 3065 4903 6324 6674 830
Size: 1710 525 1838 1421 350 3478
Fragments arranged by size:
3478 1838 1710 1421 525 350
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9322
PvuII CAG'CTG
Cuts at: 2207 3665 4114 4354 4745 2207
Size: 1458 449 240 391 6784
Fragments arranged by size:
6784 1458 449 391 240
SacI G_AGCT'C
Cuts at: 2905 2905
Size: 9322
SalI G'TCGA_C
Cuts at: 1936 6664 1936
Size: 4728 4594
SapI GCTCTTCn'nnn_
Cuts at: 2810 4606 5413 2810
Size: 1796 807 6719
Fragments arranged by size:
6719 1796 807
SbfI CC_TGCA'GG
Cuts at: 6674 6674
Size: 9322
ScaI AGT'ACT
Cuts at: 4381 7570 9282 4381
Size: 3189 1712 4421
Fragments arranged by size:
4421 3189 1712
SfcI C'TryA_G
Cuts at: 826 1045 2154 2536 2964 2994 3061 4899 6320
Size: 219 1109 382 428 30 67 1838 1421
Cuts at: 6320 6385 6670 7805 8486 8677 826
Size: 65 285 1135 681 191 1471
Fragments arranged by size:
1838 1471 1421 1135 1109 681 428 382
285 219 191 67 65 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9322
SmaI CCC'GGG
Cuts at: 1872 6649 1872
Size: 4777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5079 5286 5418 6123 7426 8327
Size: 197 926 3136 207 132 705 1303 901
Cuts at: 8327 8589 820
Size: 262 1553
Fragments arranged by size:
3136 1553 1303 926 901 705 262 207
197 132
SnaBI TAC'GTA
Cuts at: 493 6619 7113 493
Size: 6126 494 2702
Fragments arranged by size:
6126 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9322
SphI G_CATG'C
Cuts at: 1132 6680 1132
Size: 5548 3774
SspI AAT'ATT
Cuts at: 5 52 3781 4303 7246 5
Size: 47 3729 522 2943 2081
Fragments arranged by size:
3729 2943 2081 522 47
StuI AGG'CCT
Cuts at: 1517 3059 1517
Size: 1542 7780
StyI C'CwwG_G
Cuts at: 513 1065 2250 4023 5043 5847 6589 6761 513
Size: 552 1185 1773 1020 804 742 172 3074
Fragments arranged by size:
3074 1773 1185 1020 804 742 552 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7349 7366 7519 7704 8149 325
Size: 1255 5769 17 153 185 445 1498
Fragments arranged by size:
5769 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 2878 3335
Size: 274 80 33 51 1443 471 430 457
Cuts at: 3335 4379 7568 9280 96
Size: 1044 3189 1712 138
Fragments arranged by size:
3189 1712 1443 1044 471 457 430 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2495 2527 3631 3663 9282 9314 2495
Size: 32 1104 32 5619 32 2503
Fragments arranged by size:
5619 2503 1104 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4412 1316
Size: 3096 6226
XbaI T'CTAG_A
Cuts at: 2141 4175 6658 2141
Size: 2034 2483 4805
Fragments arranged by size:
4805 2483 2034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2801 5854 2801
Size: 3053 6269
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9322
XmaI C'CCGG_G
Cuts at: 1870 6647 1870
Size: 4777 4545
XmnI GAAnn'nnTTC
Cuts at: 3516 4558 4623 7451 3516
Size: 1042 65 2828 5387
Fragments arranged by size:
5387 2828 1042 65
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7130 276
Size: 53 83 186 936 5596 2468
Fragments arranged by size:
5596 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI