Restriction map for
pFN21ASDA0817
(Circular) (Six-base) MAPSORT of: pFN21ASDA0817.seq Check: 6975 from: 1 to: 13063
pFN21ASDA0817 13063 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2116 2579 3001 3145 3688 4442 4449 4751 6095
Size: 463 422 144 543 754 7 302 1344
Cuts at: 6095 2116
Size: 9084
Fragments arranged by size:
9084 1344 754 543 463 422 302 144
7
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7603 10873 278
Size: 53 83 186 936 6067 3270 2468
Fragments arranged by size:
6067 3270 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6855 7597 10406 1937
Size: 4918 742 2809 4594
Fragments arranged by size:
4918 4594 2809 742
Acc65I G'GTAC_C
Cuts at: 4773 10384 4773
Size: 5611 7452
AclI AA'CG_TT
Cuts at: 1563 11190 11563 1563
Size: 9627 373 3063
Fragments arranged by size:
9627 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2374 2935 3332 3350 3859 4661 7178 7491
Size: 742 561 397 18 509 802 2517 313
Cuts at: 7491 7915 11126 12510 12778 1632
Size: 424 3211 1384 268 1917
Fragments arranged by size:
3211 2517 1917 1384 802 742 561 509
424 397 313 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12866
AflIII A'CryG_T
Cuts at: 1203 2940 3568 5634 6882 8309 8984 11962 12939
Size: 1737 628 2066 1248 1427 675 2978 977
Cuts at: 12939 1203
Size: 1327
Fragments arranged by size:
2978 2066 1737 1427 1327 1248 977 675
628
AgeI A'CCGG_T
Cuts at: 11894 11894
Size: 13063
AhdI GACnn_n'nnGTC
Cuts at: 5781 8847 9009 9187 11792 5781
Size: 3066 162 178 2605 7052
Fragments arranged by size:
7052 3066 2605 178 162
AleI CACnn'nnGTG
Cuts at: 1166 4881 5633 6365 6797 7211 9278 9353 1166
Size: 3715 752 732 432 414 2067 75 4876
Fragments arranged by size:
4876 3715 2067 752 732 432 414 75
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2393 7807 8102 8215 1184
Size: 1209 5414 295 113 6032
Fragments arranged by size:
6032 5414 1209 295 113
AlwNI CAG_nnn'CTG
Cuts at: 2131 3506 3732 3992 4265 4681 5331 5959 6217
Size: 1375 226 260 273 416 650 628 258
Cuts at: 6217 6424 6478 6766 6769 8098 8664 9727 9847
Size: 207 54 288 3 1329 566 1063 120
Cuts at: 9847 10806 12378 2131
Size: 959 1572 2816
Fragments arranged by size:
2816 1572 1375 1329 1063 959 650 628
566 416 288 273 260 258 226 207
120 54 3
ApaI G_GGCC'C
Cuts at: 2098 3015 3356 4950 5348 6056 6254 6503 7329
Size: 917 341 1594 398 708 198 249 826
Cuts at: 7329 9450 9696 10050 10122 10194 2098
Size: 2121 246 354 72 72 4967
Fragments arranged by size:
4967 2121 1594 917 826 708 398 354
341 249 246 198 72 72
ApaLI G'TGCA_C
Cuts at: 2263 3422 3926 7831 8950 11120 12276 2263
Size: 1159 504 3905 1119 2170 1156 3050
Fragments arranged by size:
3905 3050 2170 1159 1156 1119 504
ApoI r'AATT_y
Cuts at: 1493 9269 10372 10598 10750 12859 1493
Size: 7776 1103 226 152 2109 1697
Fragments arranged by size:
7776 2109 1697 1103 226 152
AseI AT'TA_AT
Cuts at: 160 1032 11617 160
Size: 872 10585 1606
Fragments arranged by size:
10585 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13063
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2449 3007 3738 5275 5301 5580
Size: 73 154 352 558 731 1537 26 279
Cuts at: 5580 5771 6056 6929 7029 9450 10130 10388 1870
Size: 191 285 873 100 2421 680 258 4545
Fragments arranged by size:
4545 2421 1537 873 731 680 558 352
285 279 258 191 154 100 73 26
AvrII C'CTAG_G
Cuts at: 3947 5236 3947
Size: 1289 11774
BamHI G'GATC_C
Cuts at: 2229 5575 9463 10393 10760 2229
Size: 3346 3888 930 367 4532
Fragments arranged by size:
4532 3888 3346 930 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 2567 2720 2986 3655
Size: 325 217 627 353 427 153 266 669
Cuts at: 3655 3676 3759 3908 4416 4501 4634 4725 4773
Size: 21 83 149 508 85 133 91 48
Cuts at: 4773 4827 4923 5073 5537 6557 6791 7503 8217
Size: 54 96 150 464 1020 234 712 714
Cuts at: 8217 8376 9497 9698 10018 10208 10384 11839 618
Size: 159 1121 201 320 190 176 1455 1842
Fragments arranged by size:
1842 1455 1121 1020 714 712 669 627
508 464 427 353 325 320 266 234
217 201 190 176 159 153 150 149
133 96 91 85 83 54 48 21
BanII G_rGCy'C
Cuts at: 1391 2098 2982 3015 3356 4347 4459 4950 5348
Size: 707 884 33 341 991 112 491 398
Cuts at: 5348 5511 5657 6032 6056 6117 6254 6503 6811
Size: 163 146 375 24 61 137 249 308
Cuts at: 6811 6936 7167 7329 7559 8105 8526 9216 9450
Size: 125 231 162 230 546 421 690 234
Cuts at: 9450 9696 9981 10011 10050 10122 10137 10194 10382
Size: 246 285 30 39 72 15 57 188
Cuts at: 10382 1391
Size: 4072
Fragments arranged by size:
4072 991 884 707 690 546 491 421
398 375 341 308 285 249 246 234
231 230 188 163 162 146 137 125
112 72 61 57 39 33 30 24
15
BbeI G_GCGC'C
Cuts at: 2144 2724 8221 9501 9702 10212 2144
Size: 580 5497 1280 201 510 4995
Fragments arranged by size:
5497 4995 1280 580 510 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4012 7768 8865 9763 9775 928
Size: 971 2113 3756 1097 898 12 4216
Fragments arranged by size:
4216 3756 2113 1097 971 898 12
BbvCI CC'TCA_GC
Cuts at: 5108 7189 5108
Size: 2081 10982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9617 9651 11254 11288 1903
Size: 34 7680 34 1603 34 3678
Fragments arranged by size:
7680 3678 1603 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10955 12165 10955
Size: 1210 11853
BclI T'GATC_A
Cuts at: 1548 1548
Size: 13063
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2454 3869 4620
Size: 107 122 71 631 605 782 1415 751
Cuts at: 4620 4766 5021 5133 5409 6421 8333 8547 8925
Size: 146 255 112 276 1012 1912 214 378
Cuts at: 8925 9842 9976 10189 11674 136
Size: 917 134 213 1485 1525
Fragments arranged by size:
1912 1525 1485 1415 1012 917 782 751
631 605 378 276 255 214 213 146
134 122 112 107 71
BglII A'GATC_T
Cuts at: 3981 4323 13058 3981
Size: 342 8735 3986
Fragments arranged by size:
8735 3986 342
BlpI GC'TnA_GC
Cuts at: 2545 3507 5834 9709 10480 2545
Size: 962 2327 3875 771 5128
Fragments arranged by size:
5128 3875 2327 962 771
Bme1580I G_kGCm'C
Cuts at: 1790 2098 2267 2450 3015 3229 3356 3426 3660
Size: 308 169 183 565 214 127 70 234
Cuts at: 3660 3930 4272 4639 4950 5348 5469 5542 6056
Size: 270 342 367 311 398 121 73 514
Cuts at: 6056 6090 6254 6503 6546 7329 7835 8379 8954
Size: 34 164 249 43 783 506 544 575
Cuts at: 8954 9041 9450 9696 10050 10122 10194 11124 12280
Size: 87 409 246 354 72 72 930 1156
Cuts at: 12280 1790
Size: 2573
Fragments arranged by size:
2573 1156 930 783 575 565 544 514
506 409 398 367 354 342 311 308
270 249 246 234 214 183 169 164
127 121 87 73 72 72 70 43
34
BmgBI CAC'GTC
Cuts at: 1318 2621 2941 4029 5044 7926 9189 9304 1318
Size: 1303 320 1088 1015 2882 1263 115 5077
Fragments arranged by size:
5077 2882 1303 1263 1088 1015 320 115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2969 2983 3546
Size: 416 53 83 399 24 1552 14 563
Cuts at: 3546 4925 5660 5992 6201 8845 9054 10794 11752
Size: 1379 735 332 209 2644 209 1740 958
Cuts at: 11752 442
Size: 1753
Fragments arranged by size:
2644 1753 1740 1552 1379 958 735 563
416 399 332 209 209 83 53 24
14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 13063
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4000 6955 7162 8188 4000
Size: 2955 207 1026 8875
Fragments arranged by size:
8875 2955 1026 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2983 3093 4055 4361 5667 6609 6791 6985
Size: 1744 110 962 306 1306 942 182 194
Cuts at: 6985 7157 7674 8749 10160 11723 1239
Size: 172 517 1075 1411 1563 2579
Fragments arranged by size:
2579 1744 1563 1411 1306 1075 962 942
517 306 194 182 172 110
Bpu10I CC'TnA_GC
Cuts at: 3954 5108 7189 8187 3954
Size: 1154 2081 998 8830
Fragments arranged by size:
8830 2081 1154 998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 9626 11188 12053 12351 9626
Size: 1562 865 298 10338
Fragments arranged by size:
10338 1562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6290 6839 7877 9675 11726 882
Size: 617 4791 549 1038 1798 2051 2219
Fragments arranged by size:
4791 2219 2051 1798 1038 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 2268 3304 5172 6883 9553 10360 10854
Size: 711 1064 1036 1868 1711 2670 807 494
Cuts at: 10854 493
Size: 2702
Fragments arranged by size:
2702 2670 1868 1711 1064 1036 807 711
494
BsaBI GATnn'nnATC
Cuts at: 13057 13057
Size: 13063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 2721 6594 7600
Size: 53 83 186 936 608 580 3873 1006
Cuts at: 7600 7667 8218 8317 9381 9498 9699 10209 10870
Size: 67 551 99 1064 117 201 510 661
Cuts at: 10870 11252 11887 275
Size: 382 635 1451
Fragments arranged by size:
3873 1451 1064 1006 936 661 635 608
580 551 510 382 201 186 117 99
83 67 53
BsaWI w'CCGG_w
Cuts at: 2226 7605 11496 11894 12168 12315 12726 2226
Size: 5379 3891 398 274 147 411 2563
Fragments arranged by size:
5379 3891 2563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 2329 2359 2792
Size: 30 165 30 879 30 17 30 433
Cuts at: 2792 2822 3083 3113 3239 3269 3431 3461 3692
Size: 30 261 30 126 30 162 30 231
Cuts at: 3692 3722 3877 3907 4811 4841 7832 7862 8009
Size: 30 155 30 904 30 2991 30 147
Cuts at: 8009 8039 9155 9185 10158 10188 1178
Size: 30 1116 30 973 30 4053
Fragments arranged by size:
4053 2991 1116 973 904 879 433 261
231 165 162 155 147 126 30 30
30 30 30 30 30 30 30 30
30 30 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2995 3091 4136 4541 4667 5615 5765 5989
Size: 1809 96 1045 405 126 948 150 224
Cuts at: 5989 7352 8837 9486 9815 1186
Size: 1363 1485 649 329 4434
Fragments arranged by size:
4434 1809 1485 1363 1045 948 649 405
329 224 150 126 96
BseYI C'CCAG_C
Cuts at: 2074 2220 2413 2511 2657 3608 4107 4134 4797
Size: 146 193 98 146 951 499 27 663
Cuts at: 4797 5019 6135 6427 6571 7543 9127 9409 9458
Size: 222 1116 292 144 972 1584 282 49
Cuts at: 9458 9502 9670 9974 10103 10115 10280 12266 12762
Size: 44 168 304 129 12 165 1986 496
Cuts at: 12762 2074
Size: 2375
Fragments arranged by size:
2375 1986 1584 1116 972 951 663 499
496 304 292 282 222 193 168 165
146 146 144 129 98 49 44 27
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2618 3765 4055 4348 4534 5221
Size: 573 630 252 1147 290 293 186 687
Cuts at: 5221 5812 5963 6329 6929 7480 7720 7925 8452
Size: 591 151 366 600 551 240 205 527
Cuts at: 8452 9281 10235 1163
Size: 829 954 3991
Fragments arranged by size:
3991 1147 954 829 687 630 600 591
573 551 527 366 293 290 252 240
205 186 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 9241 10513 11274 12302 1540
Size: 275 186 1624 5616 1272 761 1028 2301
Fragments arranged by size:
5616 2301 1624 1272 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2267 2339 2546 2982 3426 3930 4586 5391 5770
Size: 72 207 436 444 504 656 805 379
Cuts at: 5770 6936 7835 8188 8954 9633 9825 11124 11209
Size: 1166 899 353 766 679 192 1299 85
Cuts at: 11209 12280 2267
Size: 1071 3050
Fragments arranged by size:
3050 1299 1166 1071 899 805 766 679
656 504 444 436 379 353 207 192
85 72
BsmI GAATG_Cn'
Cuts at: 3755 6086 8582 8606 10574 10667 3755
Size: 2331 2496 24 1968 93 6151
Fragments arranged by size:
6151 2496 2331 1968 93 24
BsmBI CGTCTCn'nnnn_
Cuts at: 4151 7607 8387 9026 4151
Size: 3456 780 639 8188
Fragments arranged by size:
8188 3456 780 639
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2267 2339 2450 2546 2982 3015
Size: 399 308 169 72 111 96 436 33
Cuts at: 3015 3229 3356 3426 3660 3930 4272 4347 4459
Size: 214 127 70 234 270 342 75 112
Cuts at: 4459 4586 4639 4950 5348 5391 5469 5511 5542
Size: 127 53 311 398 43 78 42 31
Cuts at: 5542 5657 5770 6032 6056 6090 6117 6254 6503
Size: 115 113 262 24 34 27 137 249
Cuts at: 6503 6546 6811 6936 7167 7329 7559 7835 8105
Size: 43 265 125 231 162 230 276 270
Cuts at: 8105 8188 8379 8526 8954 9041 9216 9450 9633
Size: 83 191 147 428 87 175 234 183
Cuts at: 9633 9696 9825 9981 10011 10050 10122 10137 10194
Size: 63 129 156 30 39 72 15 57
Cuts at: 10194 10382 11124 11209 12280 1391
Size: 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 428 399 398 342
311 308 276 270 270 265 262 249
234 234 231 230 214 191 188 183
175 169 162 156 147 137 129 127
127 125 115 113 112 111 96 87
85 83 78 75 72 72 70 63
57 53 43 43 42 39 34 33
31 30 27 24 15
BspEI T'CCGG_A
Cuts at: 7605 12726 7605
Size: 5121 7942
BspHI T'CATG_A
Cuts at: 10952 12682 10952
Size: 1730 11333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2116 2418 2579 2640 3001 3017 3145 3688
Size: 1272 302 161 61 361 16 128 543
Cuts at: 3688 4442 4449 4751 4862 5099 5231 5262 6095
Size: 754 7 302 111 237 132 31 833
Cuts at: 6095 7619 8121 8723 9669 10418 844
Size: 1524 502 602 946 749 3489
Fragments arranged by size:
3489 1524 1272 946 833 754 749 602
543 502 361 302 302 237 161 132
128 111 61 31 16 7
BsrBI CCG'CTC
Cuts at: 1394 1823 4353 4666 6460 6519 7311 10002 10950
Size: 429 2530 313 1794 59 792 2691 948
Cuts at: 10950 1394
Size: 3507
Fragments arranged by size:
3507 2691 2530 1794 948 792 429 313
59
BsrDI GCAATG_nn'
Cuts at: 65 1248 3424 4459 4903 6187 6641 7288 7732
Size: 1183 2176 1035 444 1284 454 647 444
Cuts at: 7732 8224 11558 11732 65
Size: 492 3334 174 1396
Fragments arranged by size:
3334 2176 1396 1284 1183 1035 647 492
454 444 444 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2885 3249 3624 4614 5403 5445 5585
Size: 110 1075 364 375 990 789 42 140
Cuts at: 5585 6788 7963 8444 8718 9603 9870 9970 10040
Size: 1203 1175 481 274 885 267 100 70
Cuts at: 10040 11707 11894 1700
Size: 1667 187 2869
Fragments arranged by size:
2869 1667 1203 1175 1075 990 885 789
481 375 364 274 267 187 140 110
100 70 42
BsrGI T'GTAC_A
Cuts at: 96 3714 4810 6438 9391 96
Size: 3618 1096 1628 2953 3768
Fragments arranged by size:
3768 3618 2953 1628 1096
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13063
BssSI C'ACGA_G
Cuts at: 4877 5972 6860 9626 10253 11123 12135 4877
Size: 1095 888 2766 627 870 1012 5805
Fragments arranged by size:
5805 2766 1095 1012 888 870 627
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2131 2993 3759 5826 7503 7620 8958 9727
Size: 1337 862 766 2067 1677 117 1338 769
Cuts at: 9727 10249 794
Size: 522 3608
Fragments arranged by size:
3608 2067 1677 1338 1337 862 769 766
522 117
BstBI TT'CG_AA
Cuts at: 2007 11867 2007
Size: 9860 3203
BstEII G'GTnAC_C
Cuts at: 1184 2786 9919 1184
Size: 1602 7133 4328
Fragments arranged by size:
7133 4328 1602
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2053 2657 3388 3829 4250 4409 4883 5147
Size: 24 604 731 441 421 159 474 264
Cuts at: 5147 6271 7049 7966 8969 10184 2029
Size: 1124 778 917 1003 1215 4908
Fragments arranged by size:
4908 1215 1124 1003 917 778 731 604
474 441 421 264 159 24
BstYI r'GATC_y
Cuts at: 1972 2229 3981 4233 4323 5575 9463 10393 10760
Size: 257 1752 252 90 1252 3888 930 367
Cuts at: 10760 11145 11162 12614 12700 12712 13058 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
3888 1977 1752 1452 1252 930 385 367
346 257 252 90 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2365 3531 6129 8355 2365
Size: 1166 2598 2226 7073
Fragments arranged by size:
7073 2598 2226 1166
BtgI C'CryG_G
Cuts at: 513 1065 2022 2046 2467 2563 2874 3201 3238
Size: 552 957 24 421 96 311 327 37
Cuts at: 3238 3631 3896 4823 5001 5081 5191 5482 6363
Size: 393 265 927 178 80 110 291 881
Cuts at: 6363 6553 6663 7209 7507 8419 8454 8571 9318
Size: 190 110 546 298 912 35 117 747
Cuts at: 9318 9512 9533 9589 10059 513
Size: 194 21 56 470 3517
Fragments arranged by size:
3517 957 927 912 881 747 552 546
470 421 393 327 311 298 291 265
194 190 178 117 110 110 96 80
56 37 35 24 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4854 6495 8137 487
Size: 683 56 3628 1641 1642 5413
Fragments arranged by size:
5413 3628 1642 1641 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 3258 5122 5158 5209 5329 5389 5488 5542
Size: 778 1864 36 51 120 60 99 54
Cuts at: 5542 6148 6327 7621 7835 7982 8099 8530 8626
Size: 606 179 1294 214 147 117 431 96
Cuts at: 8626 8678 9146 9493 9582 10098 10166 10235 10294
Size: 52 468 347 89 516 68 69 59
Cuts at: 10294 10579 11373 11393 2480
Size: 285 794 20 4150
Fragments arranged by size:
4150 1864 1294 794 778 606 516 468
431 347 285 214 179 147 120 117
99 96 89 69 68 60 59 54
52 51 36 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13063
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5485 5520 8717 8752 529
Size: 35 4921 35 3197 35 4840
Fragments arranged by size:
4921 4840 3197 35 35 35
DraI TTT'AAA
Cuts at: 10367 10367
Size: 13063
DraIII CAC_nnn'GTG
Cuts at: 2262 2489 2674 5483 6390 7503 9818 2262
Size: 227 185 2809 907 1113 2315 5507
Fragments arranged by size:
5507 2809 2315 1113 907 227 185
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3728 9329 9380 9568 9608 9767 12070
Size: 507 2412 5601 51 188 40 159 2303
Cuts at: 12070 809
Size: 1802
Fragments arranged by size:
5601 2412 2303 1802 507 188 159 51
40
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2067 2871
Size: 54 1425 50 275 18 213 24 804
Cuts at: 2871 3193 3622 4701 5188 5485 5742 5923 6550
Size: 322 429 1079 487 297 257 181 627
Cuts at: 6550 8130 8263 8971 9133 9238 9530 9897 10161
Size: 1580 133 708 162 105 292 367 264
Cuts at: 10161 10510 11399 13028 8
Size: 349 889 1629 43
Fragments arranged by size:
1629 1580 1425 1079 889 804 708 627
487 429 367 349 322 297 292 275
264 257 213 181 162 133 105 54
50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 9238 10510 1537
Size: 275 1810 5616 1272 4090
Fragments arranged by size:
5616 4090 1810 1272 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2393 3101 3116 4199 4637 9737 9809 10993
Size: 1040 708 15 1083 438 5100 72 1184
Cuts at: 10993 1353
Size: 3423
Fragments arranged by size:
5100 3423 1184 1083 1040 708 438 72
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2337 2800 3110 3186 4467 5271
Size: 1064 138 748 463 310 76 1281 804
Cuts at: 5271 9278 9949 9986 11650 12024 12170 387
Size: 4007 671 37 1664 374 146 1280
Fragments arranged by size:
4007 1664 1281 1280 1064 804 748 671
463 374 310 146 138 76 37
EcoICRI GAG'CTC
Cuts at: 2980 6934 2980
Size: 3954 9109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 2935 2983 3093 3332 3350 3859
Size: 393 742 561 48 110 239 18 509
Cuts at: 3859 4055 4361 4661 5667 6609 6791 6985 7157
Size: 196 306 300 1006 942 182 194 172
Cuts at: 7157 7178 7491 7674 7915 8749 10160 11126 11723
Size: 21 313 183 241 834 1411 966 597
Cuts at: 11723 12510 12778 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1411 1006 966 942 834 787 742
597 561 509 393 313 306 300 268
241 239 196 194 183 182 172 110
48 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2033 4445 6416 9830 2033
Size: 2412 1971 3414 5266
Fragments arranged by size:
5266 3414 2412 1971
EcoO109I rG'GnC_Cy
Cuts at: 2094 2209 2519 2686 2767 2833 3011 3012 3352
Size: 115 310 167 81 66 178 1 340
Cuts at: 3352 3742 3795 3902 4007 4262 4485 4558 4946
Size: 390 53 107 105 255 223 73 388
Cuts at: 4946 4947 4987 5344 5345 5473 5775 5940 5956
Size: 1 40 357 1 128 302 165 16
Cuts at: 5956 6052 6053 6250 6251 6403 6499 6613 6652
Size: 96 1 197 1 152 96 114 39
Cuts at: 6652 7325 7326 7491 7512 7731 8159 8325 8712
Size: 673 1 165 21 219 428 166 387
Cuts at: 8712 8849 9233 9447 9538 9724 10047 10055 10191
Size: 137 384 214 91 186 323 8 136
Cuts at: 10191 2094
Size: 4966
Fragments arranged by size:
4966 673 428 390 388 387 384 357
340 323 310 302 255 223 219 214
197 186 178 167 166 165 165 152
137 136 128 115 114 107 105 96
96 91 81 73 66 53 40 39
21 16 8 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 10372 10750 10372
Size: 378 12685
FseI GG_CCGG'CC
Cuts at: 3628 3628
Size: 13063
FspI TGC'GCA
Cuts at: 11569 11569
Size: 13063
HaeII r_GCGC'y
Cuts at: 1678 2144 2724 3548 4135 4177 7231 8221 9230
Size: 466 580 824 587 42 3054 990 1009
Cuts at: 9230 9501 9702 9871 10212 12210 1678
Size: 271 201 169 341 1998 2531
Fragments arranged by size:
3054 2531 1998 1009 990 824 587 580
466 341 271 201 169 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 3083 3115 3692
Size: 32 135 32 710 32 769 32 577
Cuts at: 3692 3724 5138 5170 5767 5799 8833 8865 8995
Size: 32 1414 32 597 32 3034 32 130
Cuts at: 8995 9027 9173 9205 11704 11736 11778 11810 1373
Size: 32 146 32 2499 32 42 32 2626
Fragments arranged by size:
3034 2626 2499 1414 769 710 597 577
146 135 130 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7276 7598 10407 10653 669
Size: 988 281 5338 322 2809 246 3079
Fragments arranged by size:
5338 3079 2809 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 2358 7473 748
Size: 1610 5115 6338
Fragments arranged by size:
6338 5115 1610
HpaI GTT'AAC
Cuts at: 10653 10653
Size: 13063
KasI G'GCGC_C
Cuts at: 2140 2720 8217 9497 9698 10208 2140
Size: 580 5497 1280 201 510 4995
Fragments arranged by size:
5497 4995 1280 580 510 201
KpnI G_GTAC'C
Cuts at: 4777 10388 4777
Size: 5611 7452
MfeI C'AATT_G
Cuts at: 10662 10662
Size: 13063
MluI A'CGCG_T
Cuts at: 12939 12939
Size: 13063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5874 7736 8683 12177 12361 1305
Size: 4569 1862 947 3494 184 2007
Fragments arranged by size:
4569 3494 2007 1862 947 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 3195 4703 5190 5487
Size: 54 1425 343 213 1150 1508 487 297
Cuts at: 5487 8973 9899 10163 10
Size: 3486 926 264 2910
Fragments arranged by size:
3486 2910 1508 1425 1150 926 487 343
297 264 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2865 4221 4881 5633 6365 6797 7211
Size: 648 1699 1356 660 752 732 432 414
Cuts at: 7211 7964 8424 9158 9278 9353 10346 11021 11380
Size: 753 460 734 120 75 993 675 359
Cuts at: 11380 11539 518
Size: 159 2042
Fragments arranged by size:
2042 1699 1356 993 753 752 734 732
675 660 648 460 432 414 359 159
120 75
MspA1I CmG'CkG
Cuts at: 1606 2122 2314 2598 2717 3138 3621 3825 3966
Size: 516 192 284 119 421 483 204 141
Cuts at: 3966 5019 5034 5083 5131 5295 6039 6286 6548
Size: 1053 15 49 48 164 744 247 262
Cuts at: 6548 6587 6766 6781 7680 8268 8289 8436 8538
Size: 39 179 15 899 588 21 147 102
Cuts at: 8538 8583 8683 8699 9127 9535 9591 9856 10180
Size: 45 100 16 428 408 56 265 324
Cuts at: 10180 10479 11156 12304 12549 1606
Size: 299 677 1148 245 2120
Fragments arranged by size:
2120 1148 1053 899 744 677 588 516
483 428 421 408 324 299 284 265
262 247 245 204 192 179 164 147
141 119 102 100 56 49 48 45
39 21 16 15 15
NaeI GCC'GGC
Cuts at: 2887 3626 4616 5405 5447 8720 9872 9972 2887
Size: 739 990 789 42 3273 1152 100 5978
Fragments arranged by size:
5978 3273 1152 990 789 739 100 42
NarI GG'CG_CC
Cuts at: 2141 2721 8218 9498 9699 10209 2141
Size: 580 5497 1280 201 510 4995
Fragments arranged by size:
5497 4995 1280 580 510 201
NcoI C'CATG_G
Cuts at: 513 1065 2022 2046 3201 3238 5191 6363 7209
Size: 552 957 24 1155 37 1953 1172 846
Cuts at: 7209 7507 8454 10059 513
Size: 298 947 1605 3517
Fragments arranged by size:
3517 1953 1605 1172 1155 957 947 846
552 298 37 24
NdeI CA'TA_TG
Cuts at: 387 6538 387
Size: 6151 6912
NgoMIV G'CCGG_C
Cuts at: 2885 3624 4614 5403 5445 8718 9870 9970 2885
Size: 739 990 789 42 3273 1152 100 5978
Fragments arranged by size:
5978 3273 1152 990 789 739 100 42
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 13063
NotI GC'GGCC_GC
Cuts at: 10510 10510
Size: 13063
NspI r_CATG'y
Cuts at: 1132 1229 3317 3572 4611 7855 8313 8988 9045
Size: 97 2088 255 1039 3244 458 675 57
Cuts at: 9045 10351 10421 11966 1132
Size: 1306 70 1545 2229
Fragments arranged by size:
3244 2229 2088 1545 1306 1039 675 458
255 97 70 57
PasI CC'CwG_GG
Cuts at: 1348 2029 2090 2584 2782 3913 4178 4265 6418
Size: 681 61 494 198 1131 265 87 2153
Cuts at: 6418 7411 8176 8494 10050 1348
Size: 993 765 318 1556 4361
Fragments arranged by size:
4361 2153 1556 1131 993 765 681 494
318 265 198 87 61
PciI A'CATG_T
Cuts at: 3568 8309 8984 11962 3568
Size: 4741 675 2978 4669
Fragments arranged by size:
4741 4669 2978 675
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2820 3202 5483 6217 6364 6390 6664 7357
Size: 1712 382 2281 734 147 26 274 693
Cuts at: 7357 7543 1108
Size: 186 6628
Fragments arranged by size:
6628 2281 1712 734 693 382 274 186
147 26
PfoI T'CCnGG_A
Cuts at: 2250 4338 5871 7077 7134 8765 8937 9466 2250
Size: 2088 1533 1206 57 1631 172 529 5847
Fragments arranged by size:
5847 2088 1631 1533 1206 529 172 57
PmeI GTTT'AAAC
Cuts at: 10367 10367
Size: 13063
PmlI CAC'GTG
Cuts at: 3304 5172 3304
Size: 1868 11195
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11132 11164 12645 12677 11132
Size: 32 1481 32 11518
Fragments arranged by size:
11518 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2519 2686 2767 2833 3742 3795 3902 4485 4558
Size: 167 81 66 909 53 107 583 73
Cuts at: 4558 5473 5775 6613 7731 8159 8712 8849 10055
Size: 915 302 838 1118 428 553 137 1206
Cuts at: 10055 2519
Size: 5527
Fragments arranged by size:
5527 1206 1118 915 909 838 583 553
428 302 167 137 107 81 73 66
53
PshAI GACnn'nnGTC
Cuts at: 1613 2838 1613
Size: 1225 11838
PsiI TTA'TAA
Cuts at: 10633 10633
Size: 13063
PspOMI G'GGCC_C
Cuts at: 2094 3011 3352 4946 5344 6052 6250 6499 7325
Size: 917 341 1594 398 708 198 249 826
Cuts at: 7325 9446 9692 10046 10118 10190 2094
Size: 2121 246 354 72 72 4967
Fragments arranged by size:
4967 2121 1594 917 826 708 398 354
341 249 246 198 72 72
PstI C_TGCA'G
Cuts at: 830 2538 4173 4673 4872 5318 6106 7616 8091
Size: 1708 1635 500 199 446 788 1510 475
Cuts at: 8091 8925 9439 10415 830
Size: 834 514 976 3478
Fragments arranged by size:
3478 1708 1635 1510 976 834 788 514
500 475 446 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13063
PvuII CAG'CTG
Cuts at: 2717 3825 6286 6766 6781 8436 8699 10180 2717
Size: 1108 2461 480 15 1655 263 1481 5600
Fragments arranged by size:
5600 2461 1655 1481 1108 480 263 15
RsrII CG'GwC_CG
Cuts at: 5896 5896
Size: 13063
SacI G_AGCT'C
Cuts at: 2982 6936 2982
Size: 3954 9109
SacII CC_GC'GG
Cuts at: 5084 9536 9592 5084
Size: 4452 56 8555
Fragments arranged by size:
8555 4452 56
SalI G'TCGA_C
Cuts at: 1936 7596 10405 1936
Size: 5660 2809 4594
Fragments arranged by size:
5660 4594 2809
SanDI GG'GwC_CC
Cuts at: 2686 3742 3795 4485 5473 5775 10055 2686
Size: 1056 53 690 988 302 4280 5694
Fragments arranged by size:
5694 4280 1056 988 690 302 53
SapI GCTCTTCn'nnn_
Cuts at: 9737 9737
Size: 13063
SbfI CC_TGCA'GG
Cuts at: 2538 8925 10415 2538
Size: 6387 1490 5186
Fragments arranged by size:
6387 5186 1490
ScaI AGT'ACT
Cuts at: 8807 10259 11311 13023 8807
Size: 1452 1052 1712 8847
Fragments arranged by size:
8847 1712 1452 1052
SexAI A'CCwGG_T
Cuts at: 7269 7499 7269
Size: 230 12833
SfcI C'TryA_G
Cuts at: 826 1045 2173 2534 4169 4669 4868 4928 5314
Size: 219 1128 361 1635 500 199 60 386
Cuts at: 5314 5756 6102 7612 8087 8921 9435 10411 11546
Size: 442 346 1510 475 834 514 976 1135
Cuts at: 11546 12227 12418 826
Size: 681 191 1471
Fragments arranged by size:
1635 1510 1471 1135 1128 976 834 681
514 500 475 442 386 361 346 219
199 191 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 3869 8333 3869
Size: 4464 8599
SfoI GGC'GCC
Cuts at: 2142 2722 8219 9499 9700 10210 2142
Size: 580 5497 1280 201 510 4995
Fragments arranged by size:
5497 4995 1280 580 510 201
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13063
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13063
SmaI CCC'GGG
Cuts at: 1872 2099 2451 3740 5277 5303 5582 5773 6058
Size: 227 352 1289 1537 26 279 191 285
Cuts at: 6058 9452 10390 1872
Size: 3394 938 4545
Fragments arranged by size:
4545 3394 1537 1289 938 352 285 279
227 191 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 9641 10130 11167 12068 12330 820
Size: 197 926 7698 489 1037 901 262 1553
Fragments arranged by size:
7698 1553 1037 926 901 489 262 197
SnaBI TAC'GTA
Cuts at: 493 10360 10854 493
Size: 9867 494 2702
Fragments arranged by size:
9867 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13063
SphI G_CATG'C
Cuts at: 1132 4611 10421 1132
Size: 3479 5810 3774
Fragments arranged by size:
5810 3774 3479
SrfI GCCC'GGGC
Cuts at: 2099 2099
Size: 13063
SspI AAT'ATT
Cuts at: 5 52 10987 5
Size: 47 10935 2081
Fragments arranged by size:
10935 2081 47
StuI AGG'CCT
Cuts at: 1517 3021 3863 3952 4040 6711 1517
Size: 1504 842 89 88 2671 7869
Fragments arranged by size:
7869 2671 1504 842 89 88
StyI C'CwwG_G
Cuts at: 513 1065 2022 2046 2064 3201 3238 3356 3478
Size: 552 957 24 18 1137 37 118 122
Cuts at: 3478 3763 3801 3947 5191 5236 6363 7209 7507
Size: 285 38 146 1244 45 1127 846 298
Cuts at: 7507 8454 8652 8787 9915 10059 10502 513
Size: 947 198 135 1128 144 443 3074
Fragments arranged by size:
3074 1244 1137 1128 1127 957 947 846
552 443 298 285 198 146 144 135
122 118 45 38 37 24 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2649 2774 2894 3443 3484 3839 4064
Size: 1255 1069 125 120 549 41 355 225
Cuts at: 4064 4126 4568 4751 4966 6209 6787 8827 10216
Size: 62 442 183 215 1243 578 2040 1389
Cuts at: 10216 11090 11107 11260 11445 11890 325
Size: 874 17 153 185 445 1498
Fragments arranged by size:
2040 1498 1389 1255 1243 1069 874 578
549 445 442 355 225 215 185 183
153 125 120 62 41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3714 4810 6438
Size: 274 80 33 51 1443 1737 1096 1628
Cuts at: 6438 8805 9391 10257 11309 13021 96
Size: 2367 586 866 1052 1712 138
Fragments arranged by size:
2367 1737 1712 1628 1443 1096 1052 866
586 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2246 2278 2329 2361 3239 3271 3431 3463 3877
Size: 32 51 32 878 32 160 32 414
Cuts at: 3877 3909 4811 4843 7003 7035 7600 7632 7832
Size: 32 902 32 2160 32 565 32 200
Cuts at: 7832 7864 8933 8965 9155 9187 10071 10103 10158
Size: 32 1069 32 190 32 884 32 55
Cuts at: 10158 10190 13023 13055 2246
Size: 32 2833 32 2254
Fragments arranged by size:
2833 2254 2160 1069 902 884 878 565
414 200 190 160 55 51 32 32
32 32 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2792 1316
Size: 1476 11587
XbaI T'CTAG_A
Cuts at: 10399 10399
Size: 13063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6568 8258 9844 6568
Size: 1690 1586 9787
Fragments arranged by size:
9787 1690 1586
XhoI C'TCGA_G
Cuts at: 1943 10130 1943
Size: 8187 4876
XmaI C'CCGG_G
Cuts at: 1870 2097 2449 3738 5275 5301 5580 5771 6056
Size: 227 352 1289 1537 26 279 191 285
Cuts at: 6056 9450 10388 1870
Size: 3394 938 4545
Fragments arranged by size:
4545 3394 1537 1289 938 352 285 279
227 191 26
XmnI GAAnn'nnTTC
Cuts at: 9111 11192 9111
Size: 2081 10982
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7601 10871 276
Size: 53 83 186 936 6067 3270 2468
Fragments arranged by size:
6067 3270 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FalI FspAI NruI NsiI PacI PspXI PsrI SwaI