Restriction map for
pFN21ASDA0813
(Circular) (Six-base) MAPSORT of: pFN21ASDA0813.seq Check: 6415 from: 1 to: 9331
pFN21ASDA0813 9331 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2147 2852 2891 4284 4847 4964 5114 5240 2147
Size: 705 39 1393 563 117 150 126 6238
Fragments arranged by size:
6238 1393 705 563 150 126 117 39
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7141 278
Size: 53 83 186 936 5605 2468
Fragments arranged by size:
5605 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4364 6674 1937
Size: 2427 2310 4594
Fragments arranged by size:
4594 2427 2310
Acc65I G'GTAC_C
Cuts at: 6652 6652
Size: 9331
AclI AA'CG_TT
Cuts at: 1563 7458 7831 1563
Size: 5895 373 3063
Fragments arranged by size:
5895 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3629 3743 4156 6551 7394 8778 9046 1632
Size: 1997 114 413 2395 843 1384 268 1917
Fragments arranged by size:
2395 1997 1917 1384 843 413 268 114
AflII C'TTAA_G
Cuts at: 820 1017 4417 820
Size: 197 3400 5734
Fragments arranged by size:
5734 3400 197
AflIII A'CryG_T
Cuts at: 1203 2388 8230 9207 1203
Size: 1185 5842 977 1327
Fragments arranged by size:
5842 1327 1185 977
AgeI A'CCGG_T
Cuts at: 8162 8162
Size: 9331
AhdI GACnn_n'nnGTC
Cuts at: 8060 8060
Size: 9331
AleI CACnn'nnGTG
Cuts at: 1166 4231 6177 1166
Size: 3065 1946 4320
Fragments arranged by size:
4320 3065 1946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2192 3828 6301 6311 1184
Size: 1008 1636 2473 10 4204
Fragments arranged by size:
4204 2473 1636 1008 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2390 2391 2422 2423 4970 5002 2390
Size: 1 31 1 2547 32 6719
Fragments arranged by size:
6719 2547 32 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2710 3178 3232 3322 3791 6304 7074 8646 2710
Size: 468 54 90 469 2513 770 1572 3395
Fragments arranged by size:
3395 2513 1572 770 469 468 90 54
ApaI G_GGCC'C
Cuts at: 2756 4285 4710 5223 5311 6102 6274 2756
Size: 1529 425 513 88 791 172 5813
Fragments arranged by size:
5813 1529 791 513 425 172 88
ApaLI G'TGCA_C
Cuts at: 2878 7388 8544 2878
Size: 4510 1156 3665
Fragments arranged by size:
4510 3665 1156
ApoI r'AATT_y
Cuts at: 1493 2503 6640 6866 7018 9127 1493
Size: 1010 4137 226 152 2109 1697
Fragments arranged by size:
4137 2109 1697 1010 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7885 160
Size: 872 6853 1606
Fragments arranged by size:
6853 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9331
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2749 2945 6244 6656 1870
Size: 73 93 713 196 3299 412 4545
Fragments arranged by size:
4545 3299 713 412 196 93 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3960 3993 3960
Size: 33 9298
BamHI G'GATC_C
Cuts at: 2247 5625 6661 7028 2247
Size: 3378 1036 367 4550
Fragments arranged by size:
4550 3378 1036 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2103 2111 3410 3432 4613
Size: 325 217 627 316 8 1299 22 1181
Cuts at: 4613 4835 4952 5207 5869 5942 6028 6329 6652
Size: 222 117 255 662 73 86 301 323
Cuts at: 6652 8107 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1299 1181 662 627 325 323
316 301 255 222 217 117 86 73
22 8
BanII G_rGCy'C
Cuts at: 1391 2076 2274 2736 2756 4285 4710 5223 5311
Size: 685 198 462 20 1529 425 513 88
Cuts at: 5311 5411 5822 6102 6274 6285 6494 6650 1391
Size: 100 411 280 172 11 209 156 4072
Fragments arranged by size:
4072 1529 685 513 462 425 411 280
209 198 172 156 100 88 20 11
BbeI G_GCGC'C
Cuts at: 2107 3436 5873 2107
Size: 1329 2437 5565
Fragments arranged by size:
5565 2437 1329
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4673 928
Size: 971 2774 5586
Fragments arranged by size:
5586 2774 971
BbvCI CC'TCA_GC
Cuts at: 2711 4466 5352 2711
Size: 1755 886 6690
Fragments arranged by size:
6690 1755 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2878 2912 5639 5673 7522 7556 1903
Size: 34 941 34 2727 34 1849 34 3678
Fragments arranged by size:
3678 2727 1849 941 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3988 7223 8433 3988
Size: 3235 1210 4886
Fragments arranged by size:
4886 3235 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9331
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2065 2459 2769
Size: 107 122 71 631 605 393 394 310
Cuts at: 2769 2933 4884 5487 6069 6309 7942 136
Size: 164 1951 603 582 240 1633 1525
Fragments arranged by size:
1951 1633 1525 631 605 603 582 394
393 310 240 164 122 107 71
BglII A'GATC_T
Cuts at: 9326 9326
Size: 9331
BlpI GC'TnA_GC
Cuts at: 3484 6748 3484
Size: 3264 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2756 2841 2882 3293 3415 4226 4285
Size: 336 630 85 41 411 122 811 59
Cuts at: 4285 4710 4838 4955 5117 5210 5223 5311 6102
Size: 425 128 117 162 93 13 88 791
Cuts at: 6102 6201 6253 6274 6343 6500 6616 7392 8548
Size: 99 52 21 69 157 116 776 1156
Cuts at: 8548 1790
Size: 2573
Fragments arranged by size:
2573 1156 811 791 776 630 425 411
336 162 157 128 122 117 116 99
93 88 85 69 59 52 41 21
13
BmgBI CAC'GTC
Cuts at: 1318 3553 4156 4585 1318
Size: 2235 603 429 6064
Fragments arranged by size:
6064 2235 603 429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2737 3634 3705
Size: 416 53 83 399 24 1320 897 71
Cuts at: 3705 5191 5305 7062 8020 442
Size: 1486 114 1757 958 1753
Fragments arranged by size:
1757 1753 1486 1320 958 897 416 399
114 83 71 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 2591 2990 3021 3586 3707 3845 3986
Size: 1136 216 399 31 565 121 138 141
Cuts at: 3986 7991 1239
Size: 4005 2579
Fragments arranged by size:
4005 2579 1136 565 399 216 141 138
121 31
Bpu10I CC'TnA_GC
Cuts at: 2711 2812 3041 3627 4394 4466 5352 2711
Size: 101 229 586 767 72 886 6690
Fragments arranged by size:
6690 886 767 586 229 101 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2461 3347 3886 4877 7456 8321 8619 2258
Size: 203 886 539 991 2579 865 298 2970
Fragments arranged by size:
2970 2579 991 886 865 539 298 203
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2942 5608 7994 882
Size: 617 1443 2666 2386 2219
Fragments arranged by size:
2666 2386 2219 1443 617
BsaAI yAC'GTr
Cuts at: 493 1204 4992 6628 7122 493
Size: 711 3788 1636 494 2702
Fragments arranged by size:
3788 2702 1636 711 494
BsaBI GATnn'nnATC
Cuts at: 3680 9325 3680
Size: 5645 3686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2104 3075 3433 5076
Size: 53 83 186 936 571 971 358 1643
Cuts at: 5076 5870 7138 7520 8155 275
Size: 794 1268 382 635 1451
Fragments arranged by size:
1643 1451 1268 971 936 794 635 571
382 358 186 83 53
BsaWI w'CCGG_w
Cuts at: 2144 2395 3477 3834 5167 7764 8162 8436 8583
Size: 251 1082 357 1333 2597 398 274 147
Cuts at: 8583 8994 2144
Size: 411 2481
Fragments arranged by size:
2597 2481 1333 1082 411 398 357 274
251 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4008 4038 4574 4604 5321
Size: 30 165 30 2605 30 536 30 717
Cuts at: 5321 5351 1178
Size: 30 5158
Fragments arranged by size:
5158 2605 717 536 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3935 4208 4724 6596 1186
Size: 2749 273 516 1872 3921
Fragments arranged by size:
3921 2749 1872 516 273
BseYI C'CCAG_C
Cuts at: 2755 4659 4703 4880 5714 6123 8534 9030 2755
Size: 1904 44 177 834 409 2411 496 3056
Fragments arranged by size:
3056 2411 1904 834 496 409 177 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2119 2125 2369 2642 2830 3308 3788
Size: 573 383 6 244 273 188 478 480
Cuts at: 3788 6119 6577 1163
Size: 2331 458 3917
Fragments arranged by size:
3917 2331 573 480 478 458 383 273
244 188 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 6781 7542 8570 1540
Size: 275 186 68 4712 761 1028 2301
Fragments arranged by size:
4712 2301 1028 761 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 2076 2560 2750 2882 3485 3602 4997 5165 7392
Size: 484 190 132 603 117 1395 168 2227
Cuts at: 7392 7477 8548 2076
Size: 85 1071 2859
Fragments arranged by size:
2859 2227 1395 1071 603 484 190 168
132 117 85
BsmI GAATG_Cn'
Cuts at: 3193 4296 4766 6842 6935 3193
Size: 1103 470 2076 93 5589
Fragments arranged by size:
5589 2076 1103 470 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3395 5732 3395
Size: 2337 6994
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2126 2274 2560 2736 2750 2756
Size: 399 286 50 148 286 176 14 6
Cuts at: 2756 2841 2882 3293 3415 3485 3602 4226 4285
Size: 85 41 411 122 70 117 624 59
Cuts at: 4285 4710 4838 4955 4997 5117 5165 5210 5223
Size: 425 128 117 42 120 48 45 13
Cuts at: 5223 5311 5411 5822 6102 6201 6253 6274 6285
Size: 88 100 411 280 99 52 21 11
Cuts at: 6285 6343 6494 6500 6616 6650 7392 7477 8548
Size: 58 151 6 116 34 742 85 1071
Cuts at: 8548 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 624 425 411 411 399
286 286 280 176 151 148 128 122
120 117 117 116 100 99 88 85
85 70 59 58 52 50 48 45
42 41 34 21 14 13 11 6
6
BspEI T'CCGG_A
Cuts at: 3834 5167 8994 3834
Size: 1333 3827 4171
Fragments arranged by size:
4171 3827 1333
BspHI T'CATG_A
Cuts at: 7220 8950 7220
Size: 1730 7601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2147 2852 2891 2949 3240 4284 4386 4847
Size: 1303 705 39 58 291 1044 102 461
Cuts at: 4847 4964 5114 5240 5559 5574 5590 6686 844
Size: 117 150 126 319 15 16 1096 3489
Fragments arranged by size:
3489 1303 1096 1044 705 461 319 291
150 126 117 102 58 39 16 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2359 3093 3840 4518 7218 1394
Size: 429 536 734 747 678 2700 3507
Fragments arranged by size:
3507 2700 747 734 678 536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2896 4552 4689 5655 6197 7826 8000
Size: 1183 1648 1656 137 966 542 1629 174
Cuts at: 8000 65
Size: 1396
Fragments arranged by size:
1656 1648 1629 1396 1183 966 542 174
137
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 5006 5945 7975 8162 1700
Size: 110 249 2947 939 2030 187 2869
Fragments arranged by size:
2947 2869 2030 939 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 4863 5634 5973 96
Size: 4767 771 339 3454
Fragments arranged by size:
4767 3454 771 339
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9331
BssSI C'ACGA_G
Cuts at: 4385 5189 6493 7391 8403 4385
Size: 804 1304 898 1012 5313
Fragments arranged by size:
5313 1304 1012 898 804
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3900 6196 6304 794
Size: 3106 2296 108 3821
Fragments arranged by size:
3821 3106 2296 108
BstBI TT'CG_AA
Cuts at: 2007 8135 2007
Size: 6128 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9331
BstXI CCAn_nnnn'nTGG
Cuts at: 3592 3823 5449 5830 3592
Size: 231 1626 381 7093
Fragments arranged by size:
7093 1626 381 231
BstYI r'GATC_y
Cuts at: 1972 2247 3270 3621 5170 5379 5625 5931 6661
Size: 275 1023 351 1549 209 246 306 730
Cuts at: 6661 7028 7413 7430 8882 8968 8980 9326 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1549 1452 1023 730 385 367 351
346 306 275 246 209 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6325 6325
Size: 9331
BtgI C'CryG_G
Cuts at: 513 1065 2022 2151 2164 3142 3645 4251 4540
Size: 552 957 129 13 978 503 606 289
Cuts at: 4540 5579 5607 5829 5895 5953 6166 6184 513
Size: 1039 28 222 66 58 213 18 3660
Fragments arranged by size:
3660 1039 978 957 606 552 503 289
222 213 129 66 58 28 18 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4126 6576 487
Size: 683 56 2900 2450 3242
Fragments arranged by size:
3242 2900 2450 683 56
BtsI GCAGTG_nn'
Cuts at: 2711 2922 3245 4490 5269 6109 6305 6313 6847
Size: 211 323 1245 779 840 196 8 534
Cuts at: 6847 7641 7661 2711
Size: 794 20 4381
Fragments arranged by size:
4381 1245 840 794 779 534 323 211
196 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2657 2692 4993 5028 529
Size: 35 2093 35 2301 35 4832
Fragments arranged by size:
4832 2301 2093 35 35 35
DraI TTT'AAA
Cuts at: 3706 6635 3706
Size: 2929 6402
DraIII CAC_nnn'GTG
Cuts at: 2745 6347 6349 6603 2745
Size: 3602 2 254 5473
Fragments arranged by size:
5473 3602 254 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4253 4594 8338 809
Size: 507 2937 341 3744 1802
Fragments arranged by size:
3744 2937 1802 507 341
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2720 2871
Size: 54 1425 50 275 18 236 654 151
Cuts at: 2871 2934 4029 4605 5576 6169 6310 6778 7667
Size: 63 1095 576 971 593 141 468 889
Cuts at: 7667 9296 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1095 971 889 654 593 576
468 275 236 151 141 63 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2066 6778 1537
Size: 275 254 4712 4090
Fragments arranged by size:
4712 4090 275 254
EarI CTCTTCn'nnn_
Cuts at: 1353 2741 2789 7261 1353
Size: 1388 48 4472 3423
Fragments arranged by size:
4472 3423 1388 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2642 5909 7918 8292 8438 387
Size: 1064 138 1053 3267 2009 374 146 1280
Fragments arranged by size:
3267 2009 1280 1064 1053 374 146 138
EcoICRI GAG'CTC
Cuts at: 2074 2074
Size: 9331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2591 2990 3021 3586 3629 3707
Size: 393 743 216 399 31 565 43 78
Cuts at: 3707 3743 3845 3986 4156 6551 7394 7991 8778
Size: 36 102 141 170 2395 843 597 787
Cuts at: 8778 9046 1239
Size: 268 1524
Fragments arranged by size:
2395 1524 843 787 743 597 565 399
393 268 216 170 141 102 78 43
36 31
EcoNI CCTnn'n_nnAGG
Cuts at: 3284 4289 4565 3284
Size: 1005 276 8050
Fragments arranged by size:
8050 1005 276
EcoO109I rG'GnC_Cy
Cuts at: 2046 2547 3085 3229 3771 3870 4282 4707 4769
Size: 501 538 144 542 99 412 425 62
Cuts at: 4769 5307 6098 6270 6271 6406 6520 6567 2046
Size: 538 791 172 1 135 114 47 4810
Fragments arranged by size:
4810 791 542 538 538 501 425 412
172 144 135 114 99 62 47 1
EcoRI G'AATT_C
Cuts at: 6640 7018 6640
Size: 378 8953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 6565 2278
Size: 4287 5044
FspI TGC'GCA
Cuts at: 3386 3932 6622 7837 3386
Size: 546 2690 1215 4880
Fragments arranged by size:
4880 2690 1215 546
HaeII r_GCGC'y
Cuts at: 1678 2030 2107 2218 3436 5873 8478 1678
Size: 352 77 111 1218 2437 2605 2531
Fragments arranged by size:
2605 2531 2437 1218 352 111 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3034 3066 4574 4606 5147
Size: 32 135 32 1462 32 1508 32 541
Cuts at: 5147 5179 5782 5814 5891 5923 6227 6259 6374
Size: 32 603 32 77 32 304 32 115
Cuts at: 6374 6406 6554 6586 7972 8004 8046 8078 1373
Size: 32 148 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1508 1462 1386 603 541 304 148
135 115 77 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5186 5329 5527 5905 6675 6921
Size: 988 281 3248 143 198 378 770 246
Cuts at: 6921 669
Size: 3079
Fragments arranged by size:
3248 3079 988 770 378 281 246 198
143
HindIII A'AGCT_T
Cuts at: 748 2475 748
Size: 1727 7604
HpaI GTT'AAC
Cuts at: 6921 6921
Size: 9331
KasI G'GCGC_C
Cuts at: 2103 3432 5869 2103
Size: 1329 2437 5565
Fragments arranged by size:
5565 2437 1329
KpnI G_GTAC'C
Cuts at: 6656 6656
Size: 9331
MfeI C'AATT_G
Cuts at: 6930 6930
Size: 9331
MluI A'CGCG_T
Cuts at: 9207 9207
Size: 9331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2688 4799 8445 8629 1305
Size: 1383 2111 3646 184 2007
Fragments arranged by size:
3646 2111 2007 1383 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4031 4607 5578 6171 6312
Size: 54 1425 343 2199 576 971 593 141
Cuts at: 6312 10
Size: 3029
Fragments arranged by size:
3029 2199 1425 971 593 576 343 141
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 2612 3698 4231 4439 4545 5061
Size: 648 1090 356 1086 533 208 106 516
Cuts at: 5061 5685 5828 6177 7289 7648 7807 518
Size: 624 143 349 1112 359 159 2042
Fragments arranged by size:
2042 1112 1090 1086 648 624 533 516
359 356 349 208 159 143 106
MspA1I CmG'CkG
Cuts at: 1606 2281 2296 2383 2512 2532 2719 2808 3417
Size: 675 15 87 129 20 187 89 609
Cuts at: 3417 3529 3576 3823 3900 4057 4360 4450 4647
Size: 112 47 247 77 157 303 90 197
Cuts at: 4647 4659 4884 4923 5149 5295 5912 6152 6222
Size: 12 225 39 226 146 617 240 70
Cuts at: 6222 6747 7424 8572 8817 1606
Size: 525 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 617 609 525 303
247 245 240 226 225 197 187 157
146 129 112 90 89 87 77 70
47 39 20 15 12
NarI GG'CG_CC
Cuts at: 2104 3433 5870 2104
Size: 1329 2437 5565
Fragments arranged by size:
5565 2437 1329
NcoI C'CATG_G
Cuts at: 513 1065 2022 5829 6166 6184 513
Size: 552 957 3807 337 18 3660
Fragments arranged by size:
3807 3660 957 552 337 18
NdeI CA'TA_TG
Cuts at: 387 2631 387
Size: 2244 7087
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9331
NotI GC'GGCC_GC
Cuts at: 6778 6778
Size: 9331
NspI r_CATG'y
Cuts at: 1132 1229 2392 3847 5239 6689 8234 1132
Size: 97 1163 1455 1392 1450 1545 2229
Fragments arranged by size:
2229 1545 1455 1450 1392 1163 97
PasI CC'CwG_GG
Cuts at: 1348 6095 1348
Size: 4747 4584
PciI A'CATG_T
Cuts at: 2388 8230 2388
Size: 5842 3489
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4717 5027 5203 5317 1108
Size: 3609 310 176 114 5122
Fragments arranged by size:
5122 3609 310 176 114
PfoI T'CCnGG_A
Cuts at: 2998 3225 3684 4351 6414 2998
Size: 227 459 667 2063 5915
Fragments arranged by size:
5915 2063 667 459 227
PmeI GTTT'AAAC
Cuts at: 6635 6635
Size: 9331
PmlI CAC'GTG
Cuts at: 4992 4992
Size: 9331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7400 7432 8913 8945 7400
Size: 32 1481 32 7786
Fragments arranged by size:
7786 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 3229 3870 4769 6406 6567 2046
Size: 1183 641 899 1637 161 4810
Fragments arranged by size:
4810 1637 1183 899 641 161
PshAI GACnn'nnGTC
Cuts at: 1613 4360 1613
Size: 2747 6584
PsiI TTA'TAA
Cuts at: 6901 6901
Size: 9331
PspOMI G'GGCC_C
Cuts at: 2752 4281 4706 5219 5307 6098 6270 2752
Size: 1529 425 513 88 791 172 5813
Fragments arranged by size:
5813 1529 791 513 425 172 88
PstI C_TGCA'G
Cuts at: 830 2181 2295 2888 3237 4152 4383 4485 5124
Size: 1351 114 593 349 915 231 102 639
Cuts at: 5124 5264 5285 5556 5994 6290 6300 6683 830
Size: 140 21 271 438 296 10 383 3478
Fragments arranged by size:
3478 1351 915 639 593 438 383 349
296 271 231 140 114 102 21 10
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9331
PvuII CAG'CTG
Cuts at: 2281 2296 2383 2512 2532 2808 3529 3823 3900
Size: 15 87 129 20 276 721 294 77
Cuts at: 3900 4057 4360 4450 4659 4923 5149 5912 6222
Size: 157 303 90 209 264 226 763 310
Cuts at: 6222 2281
Size: 5390
Fragments arranged by size:
5390 763 721 310 303 294 276 264
226 209 157 129 90 87 77 20
15
RsrII CG'GwC_CG
Cuts at: 2062 2062
Size: 9331
SacI G_AGCT'C
Cuts at: 2076 2076
Size: 9331
SalI G'TCGA_C
Cuts at: 1936 6673 1936
Size: 4737 4594
SanDI GG'GwC_CC
Cuts at: 6406 6406
Size: 9331
SapI GCTCTTCn'nnn_
Cuts at: 2741 2741
Size: 9331
SbfI CC_TGCA'GG
Cuts at: 4152 6683 4152
Size: 2531 6800
ScaI AGT'ACT
Cuts at: 3500 7579 9291 3500
Size: 4079 1712 3540
Fragments arranged by size:
4079 3540 1712
SexAI A'CCwGG_T
Cuts at: 6426 6426
Size: 9331
SfcI C'TryA_G
Cuts at: 826 1045 2177 2291 2884 3016 3170 3233 4148
Size: 219 1132 114 593 132 154 63 915
Cuts at: 4148 4379 4481 5120 5260 5281 5359 5552 5728
Size: 231 102 639 140 21 78 193 176
Cuts at: 5728 5990 6217 6286 6296 6679 7814 8495 8686
Size: 262 227 69 10 383 1135 681 191
Cuts at: 8686 826
Size: 1471
Fragments arranged by size:
1471 1135 1132 915 681 639 593 383
262 231 227 219 193 191 176 154
140 132 114 102 78 69 63 21
10
SfiI GGCCn_nnn'nGGCC
Cuts at: 2065 2065
Size: 9331
SfoI GGC'GCC
Cuts at: 2105 3434 5871 2105
Size: 1329 2437 5565
Fragments arranged by size:
5565 2437 1329
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9331
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9331
SmaI CCC'GGG
Cuts at: 1872 2038 6658 1872
Size: 166 4620 4545
Fragments arranged by size:
4620 4545 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 2440 3362 3865 4417 4856
Size: 197 926 330 167 922 503 552 439
Cuts at: 4856 7435 8336 8598 820
Size: 2579 901 262 1553
Fragments arranged by size:
2579 1553 926 922 901 552 503 439
330 262 197 167
SnaBI TAC'GTA
Cuts at: 493 6628 7122 493
Size: 6135 494 2702
Fragments arranged by size:
6135 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9331
SphI G_CATG'C
Cuts at: 1132 5239 6689 1132
Size: 4107 1450 3774
Fragments arranged by size:
4107 3774 1450
SspI AAT'ATT
Cuts at: 5 52 7255 5
Size: 47 7203 2081
Fragments arranged by size:
7203 2081 47
StuI AGG'CCT
Cuts at: 1517 2919 3159 5138 5467 6294 6323 1517
Size: 1402 240 1979 329 827 29 4525
Fragments arranged by size:
4525 1979 1402 827 329 240 29
StyI C'CwwG_G
Cuts at: 513 1065 2022 3285 4290 4765 5829 6166 6184
Size: 552 957 1263 1005 475 1064 337 18
Cuts at: 6184 6333 6770 513
Size: 149 437 3074
Fragments arranged by size:
3074 1263 1064 1005 957 552 475 437
337 149 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2756 4651 6115 6466 7358 7375
Size: 1255 519 657 1895 1464 351 892 17
Cuts at: 7375 7528 7713 8158 325
Size: 153 185 445 1498
Fragments arranged by size:
1895 1498 1464 1255 892 657 519 445
351 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3498 4863 5634
Size: 274 80 33 51 1443 1521 1365 771
Cuts at: 5634 5973 7577 9289 96
Size: 339 1604 1712 138
Fragments arranged by size:
1712 1604 1521 1443 1365 771 339 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2139 2171 4008 4040 4333 4365 9291 9323 2139
Size: 32 1837 32 293 32 4926 32 2147
Fragments arranged by size:
4926 2147 1837 293 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3554 6410 1316
Size: 2238 2856 4237
Fragments arranged by size:
4237 2856 2238
XbaI T'CTAG_A
Cuts at: 6667 6667
Size: 9331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6116 6233 6116
Size: 117 9214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9331
XmaI C'CCGG_G
Cuts at: 1870 2036 6656 1870
Size: 166 4620 4545
Fragments arranged by size:
4620 4545 166
XmnI GAAnn'nnTTC
Cuts at: 3341 4337 7460 3341
Size: 996 3123 5212
Fragments arranged by size:
5212 3123 996
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7139 276
Size: 53 83 186 936 5605 2468
Fragments arranged by size:
5605 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
PsrI SacII SrfI SwaI