Restriction map for
pFN21ASDA0798
(Circular) (Six-base) MAPSORT of: pFN21ASDA0798.seq Check: 8956 from: 1 to: 6685
pFN21ASDA0798 6685 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4495 278
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4028 1937
Size: 2091 4594
Acc65I G'GTAC_C
Cuts at: 4006 4006
Size: 6685
AclI AA'CG_TT
Cuts at: 1563 4812 5185 1563
Size: 3249 373 3063
Fragments arranged by size:
3249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 4748 6132 6400 1632
Size: 704 2412 1384 268 1917
Fragments arranged by size:
2412 1917 1384 704 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6488
AflIII A'CryG_T
Cuts at: 1203 5584 6561 1203
Size: 4381 977 1327
Fragments arranged by size:
4381 1327 977
AgeI A'CCGG_T
Cuts at: 5516 5516
Size: 6685
AhdI GACnn_n'nnGTC
Cuts at: 5414 5414
Size: 6685
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6685
AlwNI CAG_nnn'CTG
Cuts at: 2095 4428 6000 2095
Size: 2333 1572 2780
Fragments arranged by size:
2780 2333 1572
ApaI G_GGCC'C
Cuts at: 2102 2102
Size: 6685
ApaLI G'TGCA_C
Cuts at: 4742 5898 4742
Size: 1156 5529
ApoI r'AATT_y
Cuts at: 1493 2440 2492 2555 2781 3705 3994 4220 4372
Size: 947 52 63 226 924 289 226 152
Cuts at: 4372 6481 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 947 924 289 226 226 152
63 52
AseI AT'TA_AT
Cuts at: 160 1032 5239 160
Size: 872 4207 1606
Fragments arranged by size:
4207 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 4010 1870
Size: 73 2067 4545
Fragments arranged by size:
4545 2067 73
BamHI G'GATC_C
Cuts at: 4015 4382 4015
Size: 367 6318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4006 5461 618
Size: 325 217 627 2219 1455 1842
Fragments arranged by size:
2219 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 4004 1391
Size: 711 1902 4072
Fragments arranged by size:
4072 1902 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2821 928
Size: 971 922 4792
Fragments arranged by size:
4792 971 922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4876 4910 1903
Size: 34 2939 34 3678
Fragments arranged by size:
3678 2939 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4577 5787 4577
Size: 1210 5475
BclI T'GATC_A
Cuts at: 1548 2025 2278 2686 1548
Size: 477 253 408 5547
Fragments arranged by size:
5547 477 408 253
BfrBI ATG'CAT
Cuts at: 2348 2348
Size: 6685
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5296 136
Size: 107 122 71 631 605 3624 1525
Fragments arranged by size:
3624 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2139 6680 2139
Size: 4541 2144
BlpI GC'TnA_GC
Cuts at: 4102 4102
Size: 6685
Bme1580I G_kGCm'C
Cuts at: 1790 2102 4746 5902 1790
Size: 312 2644 1156 2573
Fragments arranged by size:
2644 2573 1156 312
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 4416 5374
Size: 416 53 83 399 24 1595 1404 958
Cuts at: 5374 442
Size: 1753
Fragments arranged by size:
1753 1595 1404 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2734 2895 2979 3483 3567 3651 3819
Size: 833 662 161 84 504 84 84 168
Cuts at: 3819 5345 1239
Size: 1526 2579
Fragments arranged by size:
2579 1526 833 662 504 168 161 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4810 5675 5973 4810
Size: 865 298 5522
Fragments arranged by size:
5522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5348 882
Size: 617 3849 2219
Fragments arranged by size:
3849 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2638 3982 4476 493
Size: 711 1434 1344 494 2702
Fragments arranged by size:
2702 1434 1344 711 494
BsaBI GATnn'nnATC
Cuts at: 2280 6679 2280
Size: 4399 2286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4492 4874 5509 275
Size: 53 83 186 936 2959 382 635 1451
Fragments arranged by size:
2959 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5118 5516 5790 5937 6348 5118
Size: 398 274 147 411 5455
Fragments arranged by size:
5455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6460
Fragments arranged by size:
6460 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2223 3916 1186
Size: 910 127 1693 3955
Fragments arranged by size:
3955 1693 910 127
BseYI C'CCAG_C
Cuts at: 5888 6384 5888
Size: 496 6189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3502 3670 1163
Size: 573 1766 168 4178
Fragments arranged by size:
4178 1766 573 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4135 4896 5924 1540
Size: 275 186 2134 761 1028 2301
Fragments arranged by size:
2301 2134 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4746 4831 5902 4746
Size: 85 1071 5529
Fragments arranged by size:
5529 1071 85
BsmI GAATG_Cn'
Cuts at: 4196 4289 4196
Size: 93 6592
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 4004 4746 4831 5902 1391
Size: 399 312 1902 742 85 1071 2174
Fragments arranged by size:
2174 1902 1071 742 399 312 85
BspEI T'CCGG_A
Cuts at: 6348 6348
Size: 6685
BspHI T'CATG_A
Cuts at: 2401 2617 2689 4574 6304 2401
Size: 216 72 1885 1730 2782
Fragments arranged by size:
2782 1885 1730 216 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2160 4040 844
Size: 1316 1880 3489
Fragments arranged by size:
3489 1880 1316
BsrBI CCG'CTC
Cuts at: 1394 1823 4572 1394
Size: 429 2749 3507
Fragments arranged by size:
3507 2749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2902 3154 3574 5180 5354 65
Size: 1183 1654 252 420 1606 174 1396
Fragments arranged by size:
1654 1606 1396 1183 420 252 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5329 5516 1700
Size: 110 3519 187 2869
Fragments arranged by size:
3519 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2938 3274 3442 3610 3694 3946 96
Size: 2842 336 168 168 84 252 2835
Fragments arranged by size:
2842 2835 336 252 168 168 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6685
BssSI C'ACGA_G
Cuts at: 4745 5757 4745
Size: 1012 5673
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6685
BstBI TT'CG_AA
Cuts at: 2007 5489 2007
Size: 3482 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6685
BstYI r'GATC_y
Cuts at: 1972 2139 4015 4382 4767 4784 6236 6322 6334
Size: 167 1876 367 385 17 1452 86 12
Cuts at: 6334 6680 1972
Size: 346 1977
Fragments arranged by size:
1977 1876 1452 385 367 346 167 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2219 513
Size: 552 1154 4979
Fragments arranged by size:
4979 1154 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5946
Fragments arranged by size:
5946 683 56
BtsI GCAGTG_nn'
Cuts at: 2650 2818 3238 3406 3490 3658 3910 4201 4995
Size: 168 420 168 84 168 252 291 794
Cuts at: 4995 5015 2650
Size: 20 4320
Fragments arranged by size:
4320 794 420 291 252 168 168 168
84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2884 2919 3136 3171 3556 3591 529
Size: 35 2320 35 217 35 385 35 3623
Fragments arranged by size:
3623 2320 385 217 35 35 35 35
DraI TTT'AAA
Cuts at: 3989 3989
Size: 6685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5692 809
Size: 507 4376 1802
Fragments arranged by size:
4376 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4132 5021 6650
Size: 54 1425 50 275 18 2302 889 1629
Cuts at: 6650 8
Size: 43
Fragments arranged by size:
2302 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4132 1537
Size: 275 2320 4090
Fragments arranged by size:
4090 2320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 2834 3002 3170 3254 3422 3711 3742
Size: 959 522 168 168 84 168 289 31
Cuts at: 3742 3842 4615 1353
Size: 100 773 3423
Fragments arranged by size:
3423 959 773 522 289 168 168 168
100 84 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5272 5646 5792 387
Size: 1064 138 3683 374 146 1280
Fragments arranged by size:
3683 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2336 2734 2895 2979 3483 3567
Size: 393 440 264 398 161 84 504 84
Cuts at: 3567 3651 3819 4748 5345 6132 6400 1239
Size: 84 168 929 597 787 268 1524
Fragments arranged by size:
1524 929 787 597 504 440 398 393
268 264 168 161 84 84 84
EcoO109I rG'GnC_Cy
Cuts at: 2099 2099
Size: 6685
EcoRI G'AATT_C
Cuts at: 2781 3994 4372 2781
Size: 1213 378 5094
Fragments arranged by size:
5094 1213 378
FspI TGC'GCA
Cuts at: 5191 5191
Size: 6685
HaeII r_GCGC'y
Cuts at: 1678 5832 1678
Size: 4154 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2495 2527 2842
Size: 32 135 32 605 32 286 32 315
Cuts at: 2842 2874 3178 3210 3430 3462 3514 3546 5326
Size: 32 304 32 220 32 52 32 1780
Cuts at: 5326 5358 5400 5432 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1780 605 315 304 286 220 135
52 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2455 4029 4275 669
Size: 988 281 517 1574 246 3079
Fragments arranged by size:
3079 1574 988 517 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6685
HpaI GTT'AAC
Cuts at: 4275 4275
Size: 6685
KpnI G_GTAC'C
Cuts at: 4010 4010
Size: 6685
MfeI C'AATT_G
Cuts at: 4284 4284
Size: 6685
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 2181 5799 5983 1305
Size: 810 66 3618 184 2007
Fragments arranged by size:
3618 2007 810 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4863
Fragments arranged by size:
4863 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3142 3310 4643 5002 5161 518
Size: 648 1976 168 1333 359 159 2042
Fragments arranged by size:
2042 1976 1333 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 4101 4778 5926 6171 1606
Size: 2495 677 1148 245 2120
Fragments arranged by size:
2495 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2219 513
Size: 552 1154 4979
Fragments arranged by size:
4979 1154 552
NdeI CA'TA_TG
Cuts at: 387 2891 3227 3563 387
Size: 2504 336 336 3509
Fragments arranged by size:
3509 2504 336 336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 4132 4132
Size: 6685
NsiI A_TGCA'T
Cuts at: 2350 2350
Size: 6685
NspI r_CATG'y
Cuts at: 1132 1229 3016 3352 3399 4043 5588 1132
Size: 97 1787 336 47 644 1545 2229
Fragments arranged by size:
2229 1787 1545 644 336 97 47
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6685
PciI A'CATG_T
Cuts at: 5584 5584
Size: 6685
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6685
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6685
PsiI TTA'TAA
Cuts at: 4255 4255
Size: 6685
PspOMI G'GGCC_C
Cuts at: 2098 2098
Size: 6685
PstI C_TGCA'G
Cuts at: 830 2289 2551 4037 830
Size: 1459 262 1486 3478
Fragments arranged by size:
3478 1486 1459 262
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
SalI G'TCGA_C
Cuts at: 1936 4027 1936
Size: 2091 4594
SapI GCTCTTCn'nnn_
Cuts at: 2834 3002 3170 3842 2834
Size: 168 168 672 5677
Fragments arranged by size:
5677 672 168 168
SbfI CC_TGCA'GG
Cuts at: 4037 4037
Size: 6685
ScaI AGT'ACT
Cuts at: 4933 6645 4933
Size: 1712 4973
SfcI C'TryA_G
Cuts at: 826 1045 2143 2285 2547 3061 4033 5168 5849
Size: 219 1098 142 262 514 972 1135 681
Cuts at: 5849 6040 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1098 972 681 514 262 219
191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 4012 1872
Size: 2140 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4789 5690 5952 820
Size: 197 926 2846 901 262 1553
Fragments arranged by size:
2846 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3982 4476 493
Size: 3489 494 2702
Fragments arranged by size:
3489 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 3016 3352 4043 1132
Size: 1884 336 691 3774
Fragments arranged by size:
3774 1884 691 336
SspI AAT'ATT
Cuts at: 5 52 4609 5
Size: 47 4557 2081
Fragments arranged by size:
4557 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6685
StyI C'CwwG_G
Cuts at: 513 1065 2219 3759 4124 513
Size: 552 1154 1540 365 3074
Fragments arranged by size:
3074 1540 1154 552 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4712 4729 4882 5067 5512 325
Size: 1255 3132 17 153 185 445 1498
Fragments arranged by size:
3132 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2567 2726 2938
Size: 274 80 33 51 1443 590 159 212
Cuts at: 2938 3274 3442 3610 3694 3946 4931 6643 96
Size: 336 168 168 84 252 985 1712 138
Fragments arranged by size:
1712 1443 985 590 336 274 252 212
168 168 159 138 84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6645 6677 6645
Size: 32 6653
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6685
XbaI T'CTAG_A
Cuts at: 4021 4021
Size: 6685
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6685
XmaI C'CCGG_G
Cuts at: 1870 4010 1870
Size: 2140 4545
XmnI GAAnn'nnTTC
Cuts at: 3080 3164 3416 3500 4814 3080
Size: 84 252 84 1314 4951
Fragments arranged by size:
4951 1314 252 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4493 276
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI