Restriction map for
pFN21ASDA0797
(Circular) (Six-base) MAPSORT of: pFN21ASDA0797.seq Check: 420 from: 1 to: 8065
pFN21ASDA0797 8065 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5875 278
Size: 53 83 186 936 4339 2468
Fragments arranged by size:
4339 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2425 2762 3370 4815 5408 1937
Size: 488 337 608 1445 593 4594
Fragments arranged by size:
4594 1445 608 593 488 337
Acc65I G'GTAC_C
Cuts at: 5386 5386
Size: 8065
AclI AA'CG_TT
Cuts at: 1563 6192 6565 1563
Size: 4629 373 3063
Fragments arranged by size:
4629 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4139 4425 4803 5086 5270 6128 7512 7780
Size: 2507 286 378 283 184 858 1384 268
Cuts at: 7780 1632
Size: 1917
Fragments arranged by size:
2507 1917 1384 858 378 286 283 268
184
AflII C'TTAA_G
Cuts at: 820 1017 4744 820
Size: 197 3727 4141
Fragments arranged by size:
4141 3727 197
AflIII A'CryG_T
Cuts at: 1203 2349 6964 7941 1203
Size: 1146 4615 977 1327
Fragments arranged by size:
4615 1327 1146 977
AgeI A'CCGG_T
Cuts at: 6896 6896
Size: 8065
AhdI GACnn_n'nnGTC
Cuts at: 6794 6794
Size: 8065
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3599 1184
Size: 2415 5650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2400 2432 2400
Size: 32 8033
AlwNI CAG_nnn'CTG
Cuts at: 5109 5808 7380 5109
Size: 699 1572 5794
Fragments arranged by size:
5794 1572 699
ApaLI G'TGCA_C
Cuts at: 6122 7278 6122
Size: 1156 6909
ApoI r'AATT_y
Cuts at: 1493 2270 2797 3643 3768 3911 4061 4140 4640
Size: 777 527 846 125 143 150 79 500
Cuts at: 4640 4989 5182 5374 5600 5752 7861 1493
Size: 349 193 192 226 152 2109 1697
Fragments arranged by size:
2109 1697 846 777 527 500 349 226
193 192 152 150 143 125 79
AseI AT'TA_AT
Cuts at: 160 1032 2538 3723 6619 160
Size: 872 1506 1185 2896 1606
Fragments arranged by size:
2896 1606 1506 1185 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8065
AvaI C'yCGr_G
Cuts at: 1870 1943 5390 1870
Size: 73 3447 4545
Fragments arranged by size:
4545 3447 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2641 2674 2641
Size: 33 8032
BamHI G'GATC_C
Cuts at: 5395 5762 5395
Size: 367 7698
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5386 6841 618
Size: 325 217 627 3599 1455 1842
Fragments arranged by size:
3599 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5072 5384 1391
Size: 3681 312 4072
Fragments arranged by size:
4072 3681 312
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2288 3198 4134 928
Size: 971 389 910 936 4859
Fragments arranged by size:
4859 971 936 910 389
BbvCI CC'TCA_GC
Cuts at: 3081 3081
Size: 8065
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6256 6290 1903
Size: 34 4319 34 3678
Fragments arranged by size:
4319 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4949 5957 7167 4949
Size: 1008 1210 5847
Fragments arranged by size:
5847 1210 1008
BclI T'GATC_A
Cuts at: 1548 2131 1548
Size: 583 7482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6676 136
Size: 107 122 71 631 605 5004 1525
Fragments arranged by size:
5004 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8060 8060
Size: 8065
BlpI GC'TnA_GC
Cuts at: 3572 5482 3572
Size: 1910 6155
Bme1580I G_kGCm'C
Cuts at: 1790 6126 7282 1790
Size: 4336 1156 2573
Fragments arranged by size:
4336 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3965 5796 6754
Size: 416 53 83 399 24 2548 1831 958
Cuts at: 6754 442
Size: 1753
Fragments arranged by size:
2548 1831 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2823 3291 3963 3987 6725 1239
Size: 1584 468 672 24 2738 2579
Fragments arranged by size:
2738 2579 1584 672 468 24
Bpu10I CC'TnA_GC
Cuts at: 3081 4008 3081
Size: 927 7138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3282 6190 7055 7353 3282
Size: 2908 865 298 3994
Fragments arranged by size:
3994 2908 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6728 882
Size: 617 5229 2219
Fragments arranged by size:
5229 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4264 5362 5856 493
Size: 711 3060 1098 494 2702
Fragments arranged by size:
3060 2702 1098 711 494
BsaBI GATnn'nnATC
Cuts at: 4539 8059 4539
Size: 3520 4545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5872 6254 6889 275
Size: 53 83 186 936 4339 382 635 1451
Fragments arranged by size:
4339 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6498 6896 7170 7317 7728 6498
Size: 398 274 147 411 6835
Fragments arranged by size:
6835 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7840
Fragments arranged by size:
7840 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2664 2667 3069 4204 1186
Size: 1478 3 402 1135 5047
Fragments arranged by size:
5047 1478 1135 402 3
BseYI C'CCAG_C
Cuts at: 7268 7764 7268
Size: 496 7569
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3096 4025 4296 1163
Size: 573 1360 929 271 4932
Fragments arranged by size:
4932 1360 929 573 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 5515 6276 7304 1540
Size: 275 186 30 3484 761 1028 2301
Fragments arranged by size:
3484 2301 1028 761 275 186 30
BsiHKAI G_wGCw'C
Cuts at: 6126 6211 7282 6126
Size: 85 1071 6909
Fragments arranged by size:
6909 1071 85
BsmI GAATG_Cn'
Cuts at: 3183 5576 5669 3183
Size: 2393 93 5579
Fragments arranged by size:
5579 2393 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5072 5384 6126 6211 7282 1391
Size: 399 3282 312 742 85 1071 2174
Fragments arranged by size:
3282 2174 1071 742 399 312 85
BspEI T'CCGG_A
Cuts at: 7728 7728
Size: 8065
BspHI T'CATG_A
Cuts at: 2134 4198 5954 7684 2134
Size: 2064 1756 1730 2515
Fragments arranged by size:
2515 2064 1756 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4047 5420 844
Size: 3203 1373 3489
Fragments arranged by size:
3489 3203 1373
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 5952 1394
Size: 429 217 3912 3507
Fragments arranged by size:
3912 3507 429 217
BsrDI GCAATG_nn'
Cuts at: 65 1248 3215 4516 6560 6734 65
Size: 1183 1967 1301 2044 174 1396
Fragments arranged by size:
2044 1967 1396 1301 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 6709 6896 1700
Size: 110 220 4679 187 2869
Fragments arranged by size:
4679 2869 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 3806 96
Size: 3710 4355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8065
BssSI C'ACGA_G
Cuts at: 6125 7137 6125
Size: 1012 7053
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8065
BstBI TT'CG_AA
Cuts at: 2007 6869 2007
Size: 4862 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8065
BstXI CCAn_nnnn'nTGG
Cuts at: 4453 4453
Size: 8065
BstYI r'GATC_y
Cuts at: 1972 5395 5762 6147 6164 7616 7702 7714 8060
Size: 3423 367 385 17 1452 86 12 346
Cuts at: 8060 1972
Size: 1977
Fragments arranged by size:
3423 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3371 3371
Size: 8065
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6556
Fragments arranged by size:
6556 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4785 487
Size: 683 56 3559 3767
Fragments arranged by size:
3767 3559 683 56
BtsI GCAGTG_nn'
Cuts at: 4594 4858 5581 6375 6395 4594
Size: 264 723 794 20 6264
Fragments arranged by size:
6264 794 723 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8065
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8030
DraI TTT'AAA
Cuts at: 2113 2317 3357 4068 4911 5369 2113
Size: 204 1040 711 843 458 4809
Fragments arranged by size:
4809 1040 843 711 458 204
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7072 809
Size: 507 5756 1802
Fragments arranged by size:
5756 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 5512 6401
Size: 54 1425 50 275 18 198 3484 889
Cuts at: 6401 8030 8
Size: 1629 43
Fragments arranged by size:
3484 1629 1425 889 275 198 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 5512 1537
Size: 275 216 3484 4090
Fragments arranged by size:
4090 3484 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 2589 3058 3392 3816 4480 5995 1353
Size: 1236 469 334 424 664 1515 3423
Fragments arranged by size:
3423 1515 1236 664 469 424 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6652 7026 7172 387
Size: 1064 138 5063 374 146 1280
Fragments arranged by size:
5063 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2823 3291 3963 3987 4139 4425 4803
Size: 393 1191 468 672 24 152 286 378
Cuts at: 4803 5086 5270 6128 6725 7512 7780 1239
Size: 283 184 858 597 787 268 1524
Fragments arranged by size:
1524 1191 858 787 672 597 468 393
378 286 283 268 184 152 24
EcoO109I rG'GnC_Cy
Cuts at: 4036 4964 4036
Size: 928 7137
EcoRI G'AATT_C
Cuts at: 2270 2797 4140 4640 5374 5752 2270
Size: 527 1343 500 734 378 4583
Fragments arranged by size:
4583 1343 734 527 500 378
FspI TGC'GCA
Cuts at: 6571 6571
Size: 8065
HaeII r_GCGC'y
Cuts at: 1678 7212 1678
Size: 5534 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2148 2588 2620 3976
Size: 32 135 32 544 32 440 32 1356
Cuts at: 3976 4008 4180 4212 5217 5249 5285 5317 6706
Size: 32 172 32 1005 32 36 32 1389
Cuts at: 6706 6738 6780 6812 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1389 1356 1005 544 440 172 135
42 36 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4816 5409 5655 669
Size: 988 281 2878 593 246 3079
Fragments arranged by size:
3079 2878 988 593 281 246
HindIII A'AGCT_T
Cuts at: 748 2073 748
Size: 1325 6740
HpaI GTT'AAC
Cuts at: 5655 5655
Size: 8065
KpnI G_GTAC'C
Cuts at: 5390 5390
Size: 8065
MfeI C'AATT_G
Cuts at: 2337 2874 5664 2337
Size: 537 2790 4738
Fragments arranged by size:
4738 2790 537
MluI A'CGCG_T
Cuts at: 7941 7941
Size: 8065
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3604 7179 7363 1305
Size: 2299 3575 184 2007
Fragments arranged by size:
3575 2299 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6243
Fragments arranged by size:
6243 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2442 4383 4389 4737 6023 6382 6541
Size: 648 1276 1941 6 348 1286 359 159
Cuts at: 6541 518
Size: 2042
Fragments arranged by size:
2042 1941 1286 1276 648 359 348 159
6
MspA1I CmG'CkG
Cuts at: 1606 5481 6158 7306 7551 1606
Size: 3875 677 1148 245 2120
Fragments arranged by size:
3875 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 8065
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6556
Fragments arranged by size:
6556 957 552
NdeI CA'TA_TG
Cuts at: 387 2287 4583 387
Size: 1900 2296 3869
Fragments arranged by size:
3869 2296 1900
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 8065
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8065
NotI GC'GGCC_GC
Cuts at: 5512 5512
Size: 8065
NspI r_CATG'y
Cuts at: 1132 1229 2353 3102 5423 6968 1132
Size: 97 1124 749 2321 1545 2229
Fragments arranged by size:
2321 2229 1545 1124 749 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8065
PciI A'CATG_T
Cuts at: 2349 6964 2349
Size: 4615 3450
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3122 1108
Size: 2014 6051
PmeI GTTT'AAAC
Cuts at: 5369 5369
Size: 8065
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6134 6166 7647 7679 6134
Size: 32 1481 32 6520
Fragments arranged by size:
6520 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4036 4036
Size: 8065
PshAI GACnn'nnGTC
Cuts at: 1613 2812 1613
Size: 1199 6866
PsiI TTA'TAA
Cuts at: 4572 5635 4572
Size: 1063 7002
PstI C_TGCA'G
Cuts at: 830 4886 5417 830
Size: 4056 531 3478
Fragments arranged by size:
4056 3478 531
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8065
SalI G'TCGA_C
Cuts at: 1936 4814 5407 1936
Size: 2878 593 4594
Fragments arranged by size:
4594 2878 593
SbfI CC_TGCA'GG
Cuts at: 5417 5417
Size: 8065
ScaI AGT'ACT
Cuts at: 6313 8025 6313
Size: 1712 6353
SfcI C'TryA_G
Cuts at: 826 1045 2412 2653 3465 3531 4416 4703 4765
Size: 219 1367 241 812 66 885 287 62
Cuts at: 4765 4882 5255 5413 6548 7229 7420 826
Size: 117 373 158 1135 681 191 1471
Fragments arranged by size:
1471 1367 1135 885 812 681 373 287
241 219 191 158 117 66 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8065
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8065
SmaI CCC'GGG
Cuts at: 1872 5392 1872
Size: 3520 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3297 4744 6169 7070 7332 820
Size: 197 926 1354 1447 1425 901 262 1553
Fragments arranged by size:
1553 1447 1425 1354 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5362 5856 493
Size: 4869 494 2702
Fragments arranged by size:
4869 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8065
SphI G_CATG'C
Cuts at: 1132 5423 1132
Size: 4291 3774
SspI AAT'ATT
Cuts at: 5 52 5989 5
Size: 47 5937 2081
Fragments arranged by size:
5937 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8065
StyI C'CwwG_G
Cuts at: 513 1065 2022 2563 5504 513
Size: 552 957 541 2941 3074
Fragments arranged by size:
3074 2941 957 552 541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2505 4152 4962 6092 6109 6262 6447
Size: 1255 925 1647 810 1130 17 153 185
Cuts at: 6447 6892 325
Size: 445 1498
Fragments arranged by size:
1647 1498 1255 1130 925 810 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2635 3806 6311
Size: 274 80 33 51 1443 658 1171 2505
Cuts at: 6311 8023 96
Size: 1712 138
Fragments arranged by size:
2505 1712 1443 1171 658 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2505 2537 4675 4707 8025 8057 2505
Size: 32 2138 32 3318 32 2513
Fragments arranged by size:
3318 2513 2138 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3347 1316
Size: 2031 6034
XbaI T'CTAG_A
Cuts at: 5401 5401
Size: 8065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8065
XmaI C'CCGG_G
Cuts at: 1870 5390 1870
Size: 3520 4545
XmnI GAAnn'nnTTC
Cuts at: 2868 5047 6194 2868
Size: 2179 1147 4739
Fragments arranged by size:
4739 2179 1147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5873 276
Size: 53 83 186 936 4339 2468
Fragments arranged by size:
4339 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI