Restriction map for
pFN21ASDA0785
(Circular) (Six-base) MAPSORT of: pFN21ASDA0785.seq Check: 9779 from: 1 to: 6811
pFN21ASDA0785 6811 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3229 3487 3943 4030 3229
Size: 258 456 87 6010
Fragments arranged by size:
6010 456 258 87
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2874 4621 278
Size: 53 83 186 936 1338 1747 2468
Fragments arranged by size:
2468 1747 1338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4154 1937
Size: 2217 4594
Acc65I G'GTAC_C
Cuts at: 3267 4132 3267
Size: 865 5946
AclI AA'CG_TT
Cuts at: 1563 4938 5311 1563
Size: 3375 373 3063
Fragments arranged by size:
3375 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2333 2501 2597 2741 4874 6258 6526
Size: 449 252 168 96 144 2133 1384 268
Cuts at: 6526 1632
Size: 1917
Fragments arranged by size:
2133 1917 1384 449 268 252 168 144
96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6614
AflIII A'CryG_T
Cuts at: 1203 2619 3420 5710 6687 1203
Size: 1416 801 2290 977 1327
Fragments arranged by size:
2290 1416 1327 977 801
AgeI A'CCGG_T
Cuts at: 5642 5642
Size: 6811
AhdI GACnn_n'nnGTC
Cuts at: 5540 5540
Size: 6811
AleI CACnn'nnGTG
Cuts at: 1166 2768 1166
Size: 1602 5209
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6811
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 4028 4060 3110
Size: 32 886 32 5861
Fragments arranged by size:
5861 886 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2398 4554 6126 2398
Size: 2156 1572 3083
Fragments arranged by size:
3083 2156 1572
ApaLI G'TGCA_C
Cuts at: 2879 4868 6024 2879
Size: 1989 1156 3666
Fragments arranged by size:
3666 1989 1156
ApoI r'AATT_y
Cuts at: 1493 2243 4120 4346 4498 6607 1493
Size: 750 1877 226 152 2109 1697
Fragments arranged by size:
2109 1877 1697 750 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5365 160
Size: 872 4333 1606
Fragments arranged by size:
4333 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
AvaI C'yCGr_G
Cuts at: 1870 1943 2237 2318 2336 3410 3593 3998 4136
Size: 73 294 81 18 1074 183 405 138
Cuts at: 4136 1870
Size: 4545
Fragments arranged by size:
4545 1074 405 294 183 138 81 73
18
BamHI G'GATC_C
Cuts at: 3890 4141 4508 3890
Size: 251 367 6193
Fragments arranged by size:
6193 367 251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2684 2734 3267 3589 4132
Size: 325 217 627 897 50 533 322 543
Cuts at: 4132 5587 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 897 627 543 533 325 322
217 50
BanII G_rGCy'C
Cuts at: 1391 2048 2463 2711 3222 3354 4130 1391
Size: 657 415 248 511 132 776 4072
Fragments arranged by size:
4072 776 657 511 415 248 132
BbeI G_GCGC'C
Cuts at: 2688 2688
Size: 6811
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2489 2729 2776 2806 3783 928
Size: 971 590 240 47 30 977 3956
Fragments arranged by size:
3956 977 971 590 240 47 30
BbvCI CC'TCA_GC
Cuts at: 3570 3570
Size: 6811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2827 2861 5002 5036 1903
Size: 34 890 34 2141 34 3678
Fragments arranged by size:
3678 2141 890 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4703 5913 4703
Size: 1210 5601
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6811
BfrBI ATG'CAT
Cuts at: 2136 2136
Size: 6811
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2360 3687 5422
Size: 107 122 71 631 605 688 1327 1735
Cuts at: 5422 136
Size: 1525
Fragments arranged by size:
1735 1525 1327 688 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3579 3672 6806 3579
Size: 93 3134 3584
Fragments arranged by size:
3584 3134 93
BlpI GC'TnA_GC
Cuts at: 3353 3737 4228 3353
Size: 384 491 5936
Fragments arranged by size:
5936 491 384
Bme1580I G_kGCm'C
Cuts at: 1790 2739 2883 3594 4872 6028 1790
Size: 949 144 711 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 949 711 144
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6811
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4542 5500 442
Size: 416 53 83 399 24 3125 958 1753
Fragments arranged by size:
3125 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6811
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3358 3358
Size: 6811
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2243 3200 3247 5471 1239
Size: 821 183 957 47 2224 2579
Fragments arranged by size:
2579 2224 957 821 183 47
Bpu10I CC'TnA_GC
Cuts at: 3570 3876 3570
Size: 306 6505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3098 3770 4936 5801 6099 3098
Size: 672 1166 865 298 3810
Fragments arranged by size:
3810 1166 865 672 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2750 3329 5474 882
Size: 617 1251 579 2145 2219
Fragments arranged by size:
2219 2145 1251 617 579
BsaAI yAC'GTr
Cuts at: 493 1204 3943 4108 4602 493
Size: 711 2739 165 494 2702
Fragments arranged by size:
2739 2702 711 494 165
BsaBI GATnn'nnATC
Cuts at: 6805 6805
Size: 6811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2685 2871 3407 4062
Size: 53 83 186 936 1152 186 536 655
Cuts at: 4062 4618 5000 5635 275
Size: 556 382 635 1451
Fragments arranged by size:
1451 1152 936 655 635 556 536 382
186 186 83 53
BsaWI w'CCGG_w
Cuts at: 2192 5244 5642 5916 6063 6474 2192
Size: 3052 398 274 147 411 2529
Fragments arranged by size:
3052 2529 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 3059 3089 3110
Size: 30 165 30 1026 30 600 30 21
Cuts at: 3110 3140 4028 4058 1178
Size: 30 888 30 3931
Fragments arranged by size:
3931 1026 888 600 165 30 30 30
30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2641 3611 3746 1186
Size: 1455 970 135 4251
Fragments arranged by size:
4251 1455 970 135
BseYI C'CCAG_C
Cuts at: 2328 2413 2440 2510 2944 6014 6510 2328
Size: 85 27 70 434 3070 496 2629
Fragments arranged by size:
3070 2629 496 434 85 70 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3284 1163
Size: 573 1548 4690
Fragments arranged by size:
4690 1548 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2406 2490 3566 4261 5022 6050
Size: 275 186 405 84 1076 695 761 1028
Cuts at: 6050 1540
Size: 2301
Fragments arranged by size:
2301 1076 1028 761 695 405 275 186
84
BsiHKAI G_wGCw'C
Cuts at: 2048 2775 2861 2883 3092 3222 3354 4872 4957
Size: 727 86 22 209 130 132 1518 85
Cuts at: 4957 6028 2048
Size: 1071 2831
Fragments arranged by size:
2831 1518 1071 727 209 132 130 86
85 22
BsmI GAATG_Cn'
Cuts at: 4322 4415 4322
Size: 93 6718
BsmBI CGTCTCn'nnnn_
Cuts at: 3079 3398 3887 3079
Size: 319 489 6003
Fragments arranged by size:
6003 489 319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2463 2711 2739 2775 2861 2883
Size: 399 258 415 248 28 36 86 22
Cuts at: 2883 3092 3222 3354 3594 4130 4872 4957 6028
Size: 209 130 132 240 536 742 85 1071
Cuts at: 6028 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 536 415 399 258 248
240 209 132 130 86 85 36 28
22
BspEI T'CCGG_A
Cuts at: 2192 6474 2192
Size: 4282 2529
BspHI T'CATG_A
Cuts at: 4700 6430 4700
Size: 1730 5081
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3229 3487 3943 4030 4166 844
Size: 2385 258 456 87 136 3489
Fragments arranged by size:
3489 2385 456 258 136 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3490 4698 1394
Size: 429 1667 1208 3507
Fragments arranged by size:
3507 1667 1208 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5306 5480 65
Size: 1183 4058 174 1396
Fragments arranged by size:
4058 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2363 2912 3561 3565 3924 5455 5642
Size: 110 553 549 649 4 359 1531 187
Cuts at: 5642 1700
Size: 2869
Fragments arranged by size:
2869 1531 649 553 549 359 187 110
4
BsrGI T'GTAC_A
Cuts at: 96 3009 96
Size: 2913 3898
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6811
BssSI C'ACGA_G
Cuts at: 4871 5883 4871
Size: 1012 5799
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6811
BstBI TT'CG_AA
Cuts at: 2007 5615 2007
Size: 3608 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6811
BstXI CCAn_nnnn'nTGG
Cuts at: 3283 3445 3283
Size: 162 6649
BstYI r'GATC_y
Cuts at: 1972 3579 3672 3890 4141 4508 4893 4910 6362
Size: 1607 93 218 251 367 385 17 1452
Cuts at: 6362 6448 6460 6806 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1607 1452 385 367 346 251 218
93 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2368 2703 3000 513
Size: 552 957 346 335 297 4324
Fragments arranged by size:
4324 957 552 346 335 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3118 3358 487
Size: 683 56 1892 240 3940
Fragments arranged by size:
3940 1892 683 240 56
BtsI GCAGTG_nn'
Cuts at: 2150 2883 3049 4327 5121 5141 2150
Size: 733 166 1278 794 20 3820
Fragments arranged by size:
3820 1278 794 733 166 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6811
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6776
DraI TTT'AAA
Cuts at: 4115 4115
Size: 6811
DraIII CAC_nnn'GTG
Cuts at: 2601 2601
Size: 6811
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5818 809
Size: 507 4502 1802
Fragments arranged by size:
4502 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2352 2365 2403
Size: 54 1425 50 275 18 522 13 38
Cuts at: 2403 2487 2586 2661 3003 3096 3243 3360 3435
Size: 84 99 75 342 93 147 117 75
Cuts at: 3435 3563 3666 3858 4258 5147 6776 8
Size: 128 103 192 400 889 1629 43
Fragments arranged by size:
1629 1425 889 522 400 342 275 192
147 128 117 103 99 93 84 75
75 54 50 43 38 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2403 2487 3563 4258 1537
Size: 275 591 84 1076 695 4090
Fragments arranged by size:
4090 1076 695 591 275 84
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 3513 3702 3818 3890 4741 1353
Size: 1383 777 189 116 72 851 3423
Fragments arranged by size:
3423 1383 851 777 189 116 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2768 3469 3883 4007 5398 5772
Size: 1064 138 1179 701 414 124 1391 374
Cuts at: 5772 5918 387
Size: 146 1280
Fragments arranged by size:
1391 1280 1179 1064 701 414 374 146
138 124
EcoICRI GAG'CTC
Cuts at: 2046 3220 3352 2046
Size: 1174 132 5505
Fragments arranged by size:
5505 1174 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2081 2243 2333 2501 2597 2741
Size: 393 428 21 162 90 168 96 144
Cuts at: 2741 3200 3247 4874 5471 6258 6526 1239
Size: 459 47 1627 597 787 268 1524
Fragments arranged by size:
1627 1524 787 597 459 428 393 268
168 162 144 96 90 47 21
EcoNI CCTnn'n_nnAGG
Cuts at: 4031 4031
Size: 6811
EcoO109I rG'GnC_Cy
Cuts at: 3529 4024 3529
Size: 495 6316
EcoRI G'AATT_C
Cuts at: 2243 4120 4498 2243
Size: 1877 378 4556
Fragments arranged by size:
4556 1877 378
FseI GG_CCGG'CC
Cuts at: 2367 2367
Size: 6811
FspI TGC'GCA
Cuts at: 5317 5317
Size: 6811
HaeII r_GCGC'y
Cuts at: 1678 2688 2808 2954 3112 3915 5958 1678
Size: 1010 120 146 158 803 2043 2531
Fragments arranged by size:
2531 2043 1010 803 158 146 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2620 2652 3239 3271 5452
Size: 32 135 32 1048 32 587 32 2181
Cuts at: 5452 5484 5526 5558 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2181 1048 587 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4155 4401 669
Size: 988 281 2217 246 3079
Fragments arranged by size:
3079 2217 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6811
HpaI GTT'AAC
Cuts at: 4401 4401
Size: 6811
KasI G'GCGC_C
Cuts at: 2684 2684
Size: 6811
KpnI G_GTAC'C
Cuts at: 3271 4136 3271
Size: 865 5946
MfeI C'AATT_G
Cuts at: 4410 4410
Size: 6811
MluI A'CGCG_T
Cuts at: 6687 6687
Size: 6811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2229 5925 6109 1305
Size: 924 3696 184 2007
Fragments arranged by size:
3696 2007 924 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3005 3098 3362 3437 10
Size: 54 1425 343 1173 93 264 75 3384
Fragments arranged by size:
3384 1425 1173 343 264 93 75 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2618 2768 3011 3059 3281 4769
Size: 648 1005 447 150 243 48 222 1488
Cuts at: 4769 5128 5287 518
Size: 359 159 2042
Fragments arranged by size:
2042 1488 1005 648 447 359 243 222
159 150 48
MspA1I CmG'CkG
Cuts at: 1606 2715 3295 4068 4227 4904 6052 6297 1606
Size: 1109 580 773 159 677 1148 245 2120
Fragments arranged by size:
2120 1148 1109 773 677 580 245 159
NaeI GCC'GGC
Cuts at: 2365 2914 3567 2365
Size: 549 653 5609
Fragments arranged by size:
5609 653 549
NarI GG'CG_CC
Cuts at: 2685 2685
Size: 6811
NcoI C'CATG_G
Cuts at: 513 1065 2022 2703 513
Size: 552 957 681 4621
Fragments arranged by size:
4621 957 681 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6811
NgoMIV G'CCGG_C
Cuts at: 2363 2912 3565 2363
Size: 549 653 5609
Fragments arranged by size:
5609 653 549
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6811
NotI GC'GGCC_GC
Cuts at: 4258 4258
Size: 6811
NsiI A_TGCA'T
Cuts at: 2138 2138
Size: 6811
NspI r_CATG'y
Cuts at: 1132 1229 2623 2887 3016 3058 3307 3424 3664
Size: 97 1394 264 129 42 249 117 240
Cuts at: 3664 3719 4169 5714 1132
Size: 55 450 1545 2229
Fragments arranged by size:
2229 1545 1394 450 264 249 240 129
117 97 55 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6811
PciI A'CATG_T
Cuts at: 2619 3420 5710 2619
Size: 801 2290 3720
Fragments arranged by size:
3720 2290 801
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 3192 3727 1108
Size: 1566 518 535 4192
Fragments arranged by size:
4192 1566 535 518
PfoI T'CCnGG_A
Cuts at: 2562 3444 2562
Size: 882 5929
PmeI GTTT'AAAC
Cuts at: 4115 4115
Size: 6811
PmlI CAC'GTG
Cuts at: 3943 3943
Size: 6811
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 4028 4060 4880 4912 6393 6425 3110
Size: 32 886 32 820 32 1481 32 3496
Fragments arranged by size:
3496 1481 886 820 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6811
PsiI TTA'TAA
Cuts at: 4381 4381
Size: 6811
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 6779
PstI C_TGCA'G
Cuts at: 830 2145 3108 3228 3840 4163 830
Size: 1315 963 120 612 323 3478
Fragments arranged by size:
3478 1315 963 612 323 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6811
SacI G_AGCT'C
Cuts at: 2048 3222 3354 2048
Size: 1174 132 5505
Fragments arranged by size:
5505 1174 132
SalI G'TCGA_C
Cuts at: 1936 4153 1936
Size: 2217 4594
SbfI CC_TGCA'GG
Cuts at: 4163 4163
Size: 6811
ScaI AGT'ACT
Cuts at: 2984 3845 5059 6771 2984
Size: 861 1214 1712 3024
Fragments arranged by size:
3024 1712 1214 861
SexAI A'CCwGG_T
Cuts at: 2597 2597
Size: 6811
SfcI C'TryA_G
Cuts at: 826 1045 2141 3104 3224 3836 4159 5294 5975
Size: 219 1096 963 120 612 323 1135 681
Cuts at: 5975 6166 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1096 963 681 612 323 219
191 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 2360 2360
Size: 6811
SfoI GGC'GCC
Cuts at: 2686 2686
Size: 6811
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6811
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6811
SmaI CCC'GGG
Cuts at: 1872 2338 4138 1872
Size: 466 1800 4545
Fragments arranged by size:
4545 1800 466
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3113 3785 4915 5816 6078 820
Size: 197 926 1170 672 1130 901 262 1553
Fragments arranged by size:
1553 1170 1130 926 901 672 262 197
SnaBI TAC'GTA
Cuts at: 493 4108 4602 493
Size: 3615 494 2702
Fragments arranged by size:
3615 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6811
SphI G_CATG'C
Cuts at: 1132 3058 3719 4169 1132
Size: 1926 661 450 3774
Fragments arranged by size:
3774 1926 661 450
SrfI GCCC'GGGC
Cuts at: 2338 2338
Size: 6811
SspI AAT'ATT
Cuts at: 5 52 4735 5
Size: 47 4683 2081
Fragments arranged by size:
4683 2081 47
StuI AGG'CCT
Cuts at: 1517 2927 1517
Size: 1410 5401
StyI C'CwwG_G
Cuts at: 513 1065 2022 2532 2703 2808 3345 3363 3378
Size: 552 957 510 171 105 537 18 15
Cuts at: 3378 3525 3828 4050 4250 513
Size: 147 303 222 200 3074
Fragments arranged by size:
3074 957 552 537 510 303 222 200
171 147 105 18 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3353 4838 4855 5008 5193 5638 325
Size: 1255 1773 1485 17 153 185 445 1498
Fragments arranged by size:
1773 1498 1485 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 2982 3009
Size: 274 80 33 51 1443 543 462 27
Cuts at: 3009 3843 5057 6769 96
Size: 834 1214 1712 138
Fragments arranged by size:
1712 1443 1214 834 543 462 274 138
80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6771 6803 6771
Size: 32 6779
Tth111I GACn'n_nGTC
Cuts at: 1316 3542 1316
Size: 2226 4585
XbaI T'CTAG_A
Cuts at: 4147 4147
Size: 6811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3208 3280 3532 3761 3208
Size: 72 252 229 6258
Fragments arranged by size:
6258 252 229 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6811
XmaI C'CCGG_G
Cuts at: 1870 2336 4136 1870
Size: 466 1800 4545
Fragments arranged by size:
4545 1800 466
XmnI GAAnn'nnTTC
Cuts at: 2105 2726 4940 2105
Size: 621 2214 3976
Fragments arranged by size:
3976 2214 621
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2872 4619 276
Size: 53 83 186 936 1338 1747 2468
Fragments arranged by size:
2468 1747 1338 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I
EcoRV FalI FspAI NruI PacI PpuMI PspOMI PspXI
PvuII RsrII SacII SanDI SapI SwaI