Restriction map for
pFN21ASDA0780
(Circular) (Six-base) MAPSORT of: pFN21ASDA0780.seq Check: 1834 from: 1 to: 7870
pFN21ASDA0780 7870 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3328 4840 3328
Size: 1512 6358
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5680 278
Size: 53 83 186 936 4144 2468
Fragments arranged by size:
4144 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 3494 5213 1937
Size: 1218 339 1719 4594
Fragments arranged by size:
4594 1719 1218 339
Acc65I G'GTAC_C
Cuts at: 5191 5191
Size: 7870
AclI AA'CG_TT
Cuts at: 1563 5997 6370 1563
Size: 4434 373 3063
Fragments arranged by size:
4434 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 3105 3291 4838 5933 7317 7585 1632
Size: 430 1043 186 1547 1095 1384 268 1917
Fragments arranged by size:
1917 1547 1384 1095 1043 430 268 186
AflII C'TTAA_G
Cuts at: 820 1017 3388 4316 820
Size: 197 2371 928 4374
Fragments arranged by size:
4374 2371 928 197
AflIII A'CryG_T
Cuts at: 1203 4914 6769 7746 1203
Size: 3711 1855 977 1327
Fragments arranged by size:
3711 1855 1327 977
AgeI A'CCGG_T
Cuts at: 6701 6701
Size: 7870
AhdI GACnn_n'nnGTC
Cuts at: 4616 6599 4616
Size: 1983 5887
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4201 1184
Size: 3017 4853
AlwNI CAG_nnn'CTG
Cuts at: 5613 7185 5613
Size: 1572 6298
ApaI G_GGCC'C
Cuts at: 4345 4829 4345
Size: 484 7386
ApaLI G'TGCA_C
Cuts at: 2944 5927 7083 2944
Size: 2983 1156 3731
Fragments arranged by size:
3731 2983 1156
ApoI r'AATT_y
Cuts at: 1493 2825 2896 2996 3349 5056 5179 5405 5557
Size: 1332 71 100 353 1707 123 226 152
Cuts at: 5557 7666 1493
Size: 2109 1697
Fragments arranged by size:
2109 1707 1697 1332 353 226 152 123
100 71
AseI AT'TA_AT
Cuts at: 160 1032 2436 2619 5118 6424 160
Size: 872 1404 183 2499 1306 1606
Fragments arranged by size:
2499 1606 1404 1306 872 183
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 5195 1870
Size: 73 1569 1683 4545
Fragments arranged by size:
4545 1683 1569 73
BamHI G'GATC_C
Cuts at: 4940 5200 5567 4940
Size: 260 367 7243
Fragments arranged by size:
7243 367 260
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2413 3735 3793 5191 6646
Size: 325 217 627 626 1322 58 1398 1455
Cuts at: 6646 618
Size: 1842
Fragments arranged by size:
1842 1455 1398 1322 627 626 325 217
58
BanII G_rGCy'C
Cuts at: 1391 2146 2643 4345 4829 5189 1391
Size: 755 497 1702 484 360 4072
Fragments arranged by size:
4072 1702 755 497 484 360
BbeI G_GCGC'C
Cuts at: 3739 3797 3739
Size: 58 7812
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2579 2604 3414 928
Size: 971 680 25 810 5384
Fragments arranged by size:
5384 971 810 680 25
BbvCI CC'TCA_GC
Cuts at: 3287 4836 3287
Size: 1549 6321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6061 6095 1903
Size: 34 4124 34 3678
Fragments arranged by size:
4124 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5762 6972 5762
Size: 1210 6660
BclI T'GATC_A
Cuts at: 1548 2832 3052 1548
Size: 1284 220 6366
Fragments arranged by size:
6366 1284 220
BfrBI ATG'CAT
Cuts at: 2735 3440 4452 2735
Size: 705 1012 6153
Fragments arranged by size:
6153 1012 705
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6481 136
Size: 107 122 71 631 605 4809 1525
Fragments arranged by size:
4809 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3764 4230 7865 3764
Size: 466 3635 3769
Fragments arranged by size:
3769 3635 466
BlpI GC'TnA_GC
Cuts at: 3912 5287 3912
Size: 1375 6495
Bme1580I G_kGCm'C
Cuts at: 1790 2948 4255 4345 4546 4829 5931 7087 1790
Size: 1158 1307 90 201 283 1102 1156 2573
Fragments arranged by size:
2573 1307 1158 1156 1102 283 201 90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7870
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 3690 5601
Size: 416 53 83 399 24 2243 30 1911
Cuts at: 5601 6559 442
Size: 958 1753
Fragments arranged by size:
2243 1911 1753 958 416 399 83 53
30 24
BmtI G_CTAG'C
Cuts at: 1056 3330 1056
Size: 2274 5596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 7870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2841 3054 3741 4506 6530 1239
Size: 1602 213 687 765 2024 2579
Fragments arranged by size:
2579 2024 1602 765 687 213
Bpu10I CC'TnA_GC
Cuts at: 3287 4836 3287
Size: 1549 6321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2682 3236 5995 6860 7158 2682
Size: 554 2759 865 298 3394
Fragments arranged by size:
3394 2759 865 554 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4486 6533 882
Size: 617 2987 2047 2219
Fragments arranged by size:
2987 2219 2047 617
BsaAI yAC'GTr
Cuts at: 493 1204 5167 5661 493
Size: 711 3963 494 2702
Fragments arranged by size:
3963 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7864 7864
Size: 7870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3736 3794 5677 6059
Size: 53 83 186 936 2203 58 1883 382
Cuts at: 6059 6694 275
Size: 635 1451
Fragments arranged by size:
2203 1883 1451 936 635 382 186 83
58 53
BsaWI w'CCGG_w
Cuts at: 2665 6303 6701 6975 7122 7533 2665
Size: 3638 398 274 147 411 3002
Fragments arranged by size:
3638 3002 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2372 2402 4833 4863 1178
Size: 30 165 30 969 30 2431 30 4185
Fragments arranged by size:
4185 2431 969 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 3121 3124 3848 4320 1186
Size: 922 1013 3 724 472 4736
Fragments arranged by size:
4736 1013 922 724 472 3
BseYI C'CCAG_C
Cuts at: 2057 3655 4553 4822 5088 7073 7569 2057
Size: 1598 898 269 266 1985 496 2358
Fragments arranged by size:
2358 1985 1598 898 496 269 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2448 2904 3949 1163
Size: 573 712 456 1045 5084
Fragments arranged by size:
5084 1045 712 573 456
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4513 5320 6081 7109 1540
Size: 275 186 2512 807 761 1028 2301
Fragments arranged by size:
2512 2301 1028 807 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2948 4315 5931 6016 7087 2948
Size: 1367 1616 85 1071 3731
Fragments arranged by size:
3731 1616 1367 1071 85
BsiWI C'GTAC_G
Cuts at: 4189 4189
Size: 7870
BsmI GAATG_Cn'
Cuts at: 4289 5381 5474 4289
Size: 1092 93 6685
Fragments arranged by size:
6685 1092 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2643 2948 4255 4315 4345 4546
Size: 399 356 497 305 1307 60 30 201
Cuts at: 4546 4829 5189 5931 6016 7087 1391
Size: 283 360 742 85 1071 2174
Fragments arranged by size:
2174 1307 1071 742 497 399 360 356
305 283 201 85 60 30
BspEI T'CCGG_A
Cuts at: 2665 7533 2665
Size: 4868 3002
BspHI T'CATG_A
Cuts at: 2829 4225 5759 7489 2829
Size: 1396 1534 1730 3210
Fragments arranged by size:
3210 1730 1534 1396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3328 4840 5054 5225 844
Size: 2484 1512 214 171 3489
Fragments arranged by size:
3489 2484 1512 214 171
BsrBI CCG'CTC
Cuts at: 1394 1823 5757 1394
Size: 429 3934 3507
Fragments arranged by size:
3934 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4009 4959 6365 6539 65
Size: 1183 2761 950 1406 174 1396
Fragments arranged by size:
2761 1406 1396 1183 950 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6514 6701 1700
Size: 110 4704 187 2869
Fragments arranged by size:
4704 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7870
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7870
BssSI C'ACGA_G
Cuts at: 5930 6942 5930
Size: 1012 6858
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4458 794
Size: 3664 4206
BstBI TT'CG_AA
Cuts at: 2007 6674 2007
Size: 4667 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7870
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2819 3632 4553 2029
Size: 790 813 921 5346
Fragments arranged by size:
5346 921 813 790
BstYI r'GATC_y
Cuts at: 1972 3764 4230 4940 5200 5567 5952 5969 7421
Size: 1792 466 710 260 367 385 17 1452
Cuts at: 7421 7507 7519 7865 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1792 1452 710 466 385 367 346
260 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2178 3171 3816 4791 2178
Size: 993 645 975 5257
Fragments arranged by size:
5257 993 975 645
BtgI C'CryG_G
Cuts at: 513 1065 2022 2088 3625 4662 513
Size: 552 957 66 1537 1037 3721
Fragments arranged by size:
3721 1537 1037 957 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2315 4716 487
Size: 683 56 1089 2401 3641
Fragments arranged by size:
3641 2401 1089 683 56
BtsI GCAGTG_nn'
Cuts at: 3285 5386 6180 6200 3285
Size: 2101 794 20 4955
Fragments arranged by size:
4955 2101 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7870
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4841 4876 529
Size: 35 4277 35 3523
Fragments arranged by size:
4277 3523 35 35
DraI TTT'AAA
Cuts at: 5174 5174
Size: 7870
DraIII CAC_nnn'GTG
Cuts at: 3956 3956
Size: 7870
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3254 6877 809
Size: 507 1938 3623 1802
Fragments arranged by size:
3623 1938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2328 3542
Size: 54 1425 50 275 18 201 297 1214
Cuts at: 3542 3936 5317 6206 7835 8
Size: 394 1381 889 1629 43
Fragments arranged by size:
1629 1425 1381 1214 889 394 297 275
201 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5317 1537
Size: 275 3505 4090
Fragments arranged by size:
4090 3505 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 3180 3309 3600 4140 4952 4980 5800
Size: 1155 672 129 291 540 812 28 820
Cuts at: 5800 1353
Size: 3423
Fragments arranged by size:
3423 1155 820 812 672 540 291 129
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6457 6831 6977 387
Size: 1064 138 4868 374 146 1280
Fragments arranged by size:
4868 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2841 3054 3105 3291 3741 4506
Size: 393 430 779 213 51 186 450 765
Cuts at: 4506 4838 5933 6530 7317 7585 1239
Size: 332 1095 597 787 268 1524
Fragments arranged by size:
1524 1095 787 779 765 597 450 430
393 332 268 213 186 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3163 4145 3163
Size: 982 6888
EcoO109I rG'GnC_Cy
Cuts at: 3220 5043 3220
Size: 1823 6047
EcoRI G'AATT_C
Cuts at: 2825 5056 5179 5557 2825
Size: 2231 123 378 5138
Fragments arranged by size:
5138 2231 378 123
EcoRV GAT'ATC
Cuts at: 2983 3014 4798 2983
Size: 31 1784 6055
Fragments arranged by size:
6055 1784 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3471 3471
Size: 7870
FspI TGC'GCA
Cuts at: 6376 6376
Size: 7870
HaeII r_GCGC'y
Cuts at: 1678 3739 3797 7017 1678
Size: 2061 58 3220 2531
Fragments arranged by size:
3220 2531 2061 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2630 2662 3179 3211 3809
Size: 32 135 32 1058 32 517 32 598
Cuts at: 3809 3841 4479 4511 4602 4634 4751 4783 4833
Size: 32 638 32 91 32 117 32 50
Cuts at: 4833 4865 4953 4985 6511 6543 6585 6617 1373
Size: 32 88 32 1526 32 42 32 2626
Fragments arranged by size:
2626 1526 1058 638 598 517 135 117
91 88 50 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3594 4621 5214 5460 669
Size: 988 281 1656 1027 593 246 3079
Fragments arranged by size:
3079 1656 1027 988 593 281 246
HindIII A'AGCT_T
Cuts at: 748 3136 3432 748
Size: 2388 296 5186
Fragments arranged by size:
5186 2388 296
HpaI GTT'AAC
Cuts at: 3594 5460 3594
Size: 1866 6004
KasI G'GCGC_C
Cuts at: 3735 3793 3735
Size: 58 7812
KpnI G_GTAC'C
Cuts at: 5195 5195
Size: 7870
MfeI C'AATT_G
Cuts at: 5469 5469
Size: 7870
MluI A'CGCG_T
Cuts at: 7746 7746
Size: 7870
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6984 7168 1305
Size: 5679 184 2007
Fragments arranged by size:
5679 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2330 3544 3938 10
Size: 54 1425 343 498 1214 394 3942
Fragments arranged by size:
3942 1425 1214 498 394 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 3630 4661 5828 6187 6346 518
Size: 648 861 1603 1031 1167 359 159 2042
Fragments arranged by size:
2042 1603 1167 1031 861 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2326 3384 3659 3911 5286 5963 7111
Size: 455 265 1058 275 252 1375 677 1148
Cuts at: 7111 7356 1606
Size: 245 2120
Fragments arranged by size:
2120 1375 1148 1058 677 455 275 265
252 245
NarI GG'CG_CC
Cuts at: 3736 3794 3736
Size: 58 7812
NcoI C'CATG_G
Cuts at: 513 1065 2022 2088 3625 513
Size: 552 957 66 1537 4758
Fragments arranged by size:
4758 1537 957 552 66
NdeI CA'TA_TG
Cuts at: 387 2845 387
Size: 2458 5412
NheI G'CTAG_C
Cuts at: 1052 3326 1052
Size: 2274 5596
NotI GC'GGCC_GC
Cuts at: 5317 5317
Size: 7870
NsiI A_TGCA'T
Cuts at: 2737 3442 4454 2737
Size: 705 1012 6153
Fragments arranged by size:
6153 1012 705
NspI r_CATG'y
Cuts at: 1132 1229 2569 4605 4918 5228 6773 1132
Size: 97 1340 2036 313 310 1545 2229
Fragments arranged by size:
2229 2036 1545 1340 313 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7870
PciI A'CATG_T
Cuts at: 4914 6769 4914
Size: 1855 6015
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2649 1108
Size: 1541 6329
PfoI T'CCnGG_A
Cuts at: 2100 2100
Size: 7870
PmeI GTTT'AAAC
Cuts at: 5174 5174
Size: 7870
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5939 5971 7452 7484 3707
Size: 32 2200 32 1481 32 4093
Fragments arranged by size:
4093 2200 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3220 5043 3220
Size: 1823 6047
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7870
PsiI TTA'TAA
Cuts at: 5440 5440
Size: 7870
PspOMI G'GGCC_C
Cuts at: 4341 4825 4341
Size: 484 7386
PstI C_TGCA'G
Cuts at: 830 3105 4463 5222 830
Size: 2275 1358 759 3478
Fragments arranged by size:
3478 2275 1358 759
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7870
PvuII CAG'CTG
Cuts at: 2061 2326 3384 3911 2061
Size: 265 1058 527 6020
Fragments arranged by size:
6020 1058 527 265
SalI G'TCGA_C
Cuts at: 1936 5212 1936
Size: 3276 4594
SbfI CC_TGCA'GG
Cuts at: 5222 5222
Size: 7870
ScaI AGT'ACT
Cuts at: 2390 6118 7830 2390
Size: 3728 1712 2430
Fragments arranged by size:
3728 2430 1712
SexAI A'CCwGG_T
Cuts at: 4920 4920
Size: 7870
SfcI C'TryA_G
Cuts at: 826 1045 2611 3101 4459 5218 6353 7034 7225
Size: 219 1566 490 1358 759 1135 681 191
Cuts at: 7225 826
Size: 1471
Fragments arranged by size:
1566 1471 1358 1135 759 681 490 219
191
SfoI GGC'GCC
Cuts at: 3737 3795 3737
Size: 58 7812
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7870
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7870
SmaI CCC'GGG
Cuts at: 1872 5197 1872
Size: 3325 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2697 3251 3388 4316 5974 6875
Size: 197 926 754 554 137 928 1658 901
Cuts at: 6875 7137 820
Size: 262 1553
Fragments arranged by size:
1658 1553 928 926 901 754 554 262
197 137
SnaBI TAC'GTA
Cuts at: 493 5167 5661 493
Size: 4674 494 2702
Fragments arranged by size:
4674 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7870
SphI G_CATG'C
Cuts at: 1132 5228 1132
Size: 4096 3774
SspI AAT'ATT
Cuts at: 5 52 2345 4121 4892 4906 5794 5
Size: 47 2293 1776 771 14 888 2081
Fragments arranged by size:
2293 2081 1776 888 771 47 14
StuI AGG'CCT
Cuts at: 1517 3169 3754 1517
Size: 1652 585 5633
Fragments arranged by size:
5633 1652 585
StyI C'CwwG_G
Cuts at: 513 1065 2022 2088 2722 3625 4638 5309 513
Size: 552 957 66 634 903 1013 671 3074
Fragments arranged by size:
3074 1013 957 903 671 634 552 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4144 5897 5914 6067 6252 6697 325
Size: 1255 2564 1753 17 153 185 445 1498
Fragments arranged by size:
2564 1753 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2280 2348 2388
Size: 274 80 33 51 1443 303 68 40
Cuts at: 2388 3392 6116 7828 96
Size: 1004 2724 1712 138
Fragments arranged by size:
2724 1712 1443 1004 303 274 138 80
68 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7830 7862 7830
Size: 32 7838
Tth111I GACn'n_nGTC
Cuts at: 1316 3236 3892 1316
Size: 1920 656 5294
Fragments arranged by size:
5294 1920 656
XbaI T'CTAG_A
Cuts at: 5206 5206
Size: 7870
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7870
XmaI C'CCGG_G
Cuts at: 1870 5195 1870
Size: 3325 4545
XmnI GAAnn'nnTTC
Cuts at: 2315 5999 2315
Size: 3684 4186
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5678 276
Size: 53 83 186 936 4144 2468
Fragments arranged by size:
4144 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsmBI BstZ17I EcoICRI
FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SrfI
SwaI XcmI