Restriction map for
pFN21ASDA0776
(Circular) (Six-base) MAPSORT of: pFN21ASDA0776.seq Check: 5662 from: 1 to: 7111
pFN21ASDA0776 7111 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3043 3043
Size: 7111
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4921 278
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3813 4454 1937
Size: 1876 641 4594
Fragments arranged by size:
4594 1876 641
Acc65I G'GTAC_C
Cuts at: 4432 4432
Size: 7111
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3032 3234 3764 5174 6558 6826 1632
Size: 1400 202 530 1410 1384 268 1917
Fragments arranged by size:
1917 1410 1400 1384 530 268 202
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6914
AflIII A'CryG_T
Cuts at: 1203 2233 6010 6987 1203
Size: 1030 3777 977 1327
Fragments arranged by size:
3777 1327 1030 977
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 5840 5840
Size: 7111
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7111
AlwNI CAG_nnn'CTG
Cuts at: 2861 4015 4854 6426 2861
Size: 1154 839 1572 3546
Fragments arranged by size:
3546 1572 1154 839
ApaLI G'TGCA_C
Cuts at: 5168 6324 5168
Size: 1156 5955
ApoI r'AATT_y
Cuts at: 1493 2632 2825 3122 3852 4179 4420 4646 4798
Size: 1139 193 297 730 327 241 226 152
Cuts at: 4798 6907 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1139 730 327 297 241 226
193 152
AseI AT'TA_AT
Cuts at: 160 1032 2280 5665 160
Size: 872 1248 3385 1606
Fragments arranged by size:
3385 1606 1248 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 3563 4436 1870
Size: 73 1620 873 4545
Fragments arranged by size:
4545 1620 873 73
BamHI G'GATC_C
Cuts at: 4441 4808 4441
Size: 367 6744
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4432 5887 618
Size: 325 217 627 2645 1455 1842
Fragments arranged by size:
2645 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4430 1391
Size: 3039 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3582 3936 4068 4262 928
Size: 971 1683 354 132 194 3777
Fragments arranged by size:
3777 1683 971 354 194 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5302 5336 1903
Size: 34 3365 34 3678
Fragments arranged by size:
3678 3365 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2600 5003 6213 2600
Size: 2403 1210 3498
Fragments arranged by size:
3498 2403 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7111
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5722 136
Size: 107 122 71 631 605 4050 1525
Fragments arranged by size:
4050 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3220 7106 3220
Size: 3886 3225
BlpI GC'TnA_GC
Cuts at: 4528 4528
Size: 7111
Bme1580I G_kGCm'C
Cuts at: 1790 5172 6328 1790
Size: 3382 1156 2573
Fragments arranged by size:
3382 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7111
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4269 4842 5800
Size: 416 53 83 399 24 2852 573 958
Cuts at: 5800 442
Size: 1753
Fragments arranged by size:
2852 1753 958 573 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2755 5771 1239
Size: 942 574 3016 2579
Fragments arranged by size:
3016 2579 942 574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2705 5236 6101 6399 2705
Size: 2531 865 298 3417
Fragments arranged by size:
3417 2531 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5774 882
Size: 617 4275 2219
Fragments arranged by size:
4275 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2588 4408 4902 493
Size: 711 1384 1820 494 2702
Fragments arranged by size:
2702 1820 1384 711 494
BsaBI GATnn'nnATC
Cuts at: 7105 7105
Size: 7111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 4918 5300 5935
Size: 53 83 186 936 498 2887 382 635
Cuts at: 5935 275
Size: 1451
Fragments arranged by size:
2887 1451 936 635 498 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5544 5942 6216 6363 6774 5544
Size: 398 274 147 411 5881
Fragments arranged by size:
5881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 1178
Size: 30 165 30 1350 30 5506
Fragments arranged by size:
5506 1350 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2559 3336 3339 3521 1186
Size: 1373 777 3 182 4776
Fragments arranged by size:
4776 1373 777 182 3
BseYI C'CCAG_C
Cuts at: 6314 6810 6314
Size: 496 6615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4064 1163
Size: 573 2328 4210
Fragments arranged by size:
4210 2328 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 4561 5322 6350 1540
Size: 275 186 60 2500 761 1028 2301
Fragments arranged by size:
2500 2301 1028 761 275 186 60
BsiHKAI G_wGCw'C
Cuts at: 5172 5257 6328 5172
Size: 85 1071 5955
Fragments arranged by size:
5955 1071 85
BsmI GAATG_Cn'
Cuts at: 3065 4622 4715 3065
Size: 1557 93 5461
Fragments arranged by size:
5461 1557 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4430 5172 5257 6328 1391
Size: 399 2640 742 85 1071 2174
Fragments arranged by size:
2640 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6774 6774
Size: 7111
BspHI T'CATG_A
Cuts at: 5000 6730 5000
Size: 1730 5381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3043 4466 844
Size: 2199 1423 3489
Fragments arranged by size:
3489 2199 1423
BsrBI CCG'CTC
Cuts at: 1394 1823 2110 4998 1394
Size: 429 287 2888 3507
Fragments arranged by size:
3507 2888 429 287
BsrDI GCAATG_nn'
Cuts at: 65 1248 3343 4158 5606 5780 65
Size: 1183 2095 815 1448 174 1396
Fragments arranged by size:
2095 1448 1396 1183 815 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5755 5942 1700
Size: 110 3945 187 2869
Fragments arranged by size:
3945 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3137 3663 96
Size: 3041 526 3544
Fragments arranged by size:
3544 3041 526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 5171 6183 5171
Size: 1012 6099
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7111
BstBI TT'CG_AA
Cuts at: 2007 5915 2007
Size: 3908 3203
BstEII G'GTnAC_C
Cuts at: 1184 2431 1184
Size: 1247 5864
BstXI CCAn_nnnn'nTGG
Cuts at: 4136 4136
Size: 7111
BstYI r'GATC_y
Cuts at: 1972 3220 4441 4808 5193 5210 6662 6748 6760
Size: 1248 1221 367 385 17 1452 86 12
Cuts at: 6760 7106 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1248 1221 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3504 3504
Size: 7111
BtgI C'CryG_G
Cuts at: 513 1065 2022 2593 513
Size: 552 957 571 5031
Fragments arranged by size:
5031 957 571 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6372
Fragments arranged by size:
6372 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 4627 5421 5441 3313
Size: 1314 794 20 4983
Fragments arranged by size:
4983 1314 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 4415 4415
Size: 7111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6118 809
Size: 507 4802 1802
Fragments arranged by size:
4802 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 4558 5447
Size: 54 1425 50 275 18 228 2500 889
Cuts at: 5447 7076 8
Size: 1629 43
Fragments arranged by size:
2500 1629 1425 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 4558 1537
Size: 275 246 2500 4090
Fragments arranged by size:
4090 2500 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2391 4395 5041 1353
Size: 681 357 2004 646 3423
Fragments arranged by size:
3423 2004 681 646 357
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 5698 6072 6218 387
Size: 1064 138 453 3656 374 146 1280
Fragments arranged by size:
3656 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2755 3032 3234 3764 5174 5771
Size: 393 549 574 277 202 530 1410 597
Cuts at: 5771 6558 6826 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1410 787 597 574 549 530 393
277 268 202
EcoNI CCTnn'n_nnAGG
Cuts at: 2803 2803
Size: 7111
EcoRI G'AATT_C
Cuts at: 2632 4420 4798 2632
Size: 1788 378 4945
Fragments arranged by size:
4945 1788 378
EcoRV GAT'ATC
Cuts at: 3763 3763
Size: 7111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3905 3905
Size: 7111
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 6258 1678
Size: 4580 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2753 2785 2822 2835 2854
Size: 32 135 32 1181 32 37 13 19
Cuts at: 2854 2867 3389 3421 5752 5784 5826 5858 1373
Size: 13 522 32 2331 32 42 32 2626
Fragments arranged by size:
2626 2331 1181 522 135 42 37 32
32 32 32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4455 4701 669
Size: 988 281 2517 246 3079
Fragments arranged by size:
3079 2517 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2562 3963 748
Size: 1814 1401 3896
Fragments arranged by size:
3896 1814 1401
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KpnI G_GTAC'C
Cuts at: 4436 4436
Size: 7111
MfeI C'AATT_G
Cuts at: 4710 4710
Size: 7111
MluI A'CGCG_T
Cuts at: 6987 6987
Size: 7111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4045 6225 6409 1305
Size: 2740 2180 184 2007
Fragments arranged by size:
2740 2180 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5289
Fragments arranged by size:
5289 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5069 5428 5587 518
Size: 648 3903 359 159 2042
Fragments arranged by size:
3903 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2627 4527 5204 6352 6597 1606
Size: 465 556 1900 677 1148 245 2120
Fragments arranged by size:
2120 1900 1148 677 556 465 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5602
Fragments arranged by size:
5602 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 2237 2342 4126 4199 4469 6014 1132
Size: 97 1008 105 1784 73 270 1545 2229
Fragments arranged by size:
2229 1784 1545 1008 270 105 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7111
PciI A'CATG_T
Cuts at: 2233 6010 2233
Size: 3777 3334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7111
PfoI T'CCnGG_A
Cuts at: 2771 3456 2771
Size: 685 6426
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5180 5212 6693 6725 5180
Size: 32 1481 32 5566
Fragments arranged by size:
5566 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 3128 4681 3128
Size: 1553 5558
PstI C_TGCA'G
Cuts at: 830 3826 3958 4463 830
Size: 2996 132 505 3478
Fragments arranged by size:
3478 2996 505 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
PvuII CAG'CTG
Cuts at: 2627 2627
Size: 7111
SalI G'TCGA_C
Cuts at: 1936 4453 1936
Size: 2517 4594
SbfI CC_TGCA'GG
Cuts at: 4463 4463
Size: 7111
ScaI AGT'ACT
Cuts at: 2785 3533 4321 5359 7071 2785
Size: 748 788 1038 1712 2825
Fragments arranged by size:
2825 1712 1038 788 748
SfcI C'TryA_G
Cuts at: 826 1045 2622 3087 3822 3954 4459 5594 6275
Size: 219 1577 465 735 132 505 1135 681
Cuts at: 6275 6466 826
Size: 191 1471
Fragments arranged by size:
1577 1471 1135 735 681 505 465 219
191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 4438 1872
Size: 2566 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 3563 5215 6116 6378 820
Size: 197 926 741 879 1652 901 262 1553
Fragments arranged by size:
1652 1553 926 901 879 741 262 197
SnaBI TAC'GTA
Cuts at: 493 4408 4902 493
Size: 3915 494 2702
Fragments arranged by size:
3915 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7111
SphI G_CATG'C
Cuts at: 1132 4126 4199 4469 1132
Size: 2994 73 270 3774
Fragments arranged by size:
3774 2994 270 73
SspI AAT'ATT
Cuts at: 5 52 5035 5
Size: 47 4983 2081
Fragments arranged by size:
4983 2081 47
StuI AGG'CCT
Cuts at: 1517 3080 1517
Size: 1563 5548
StyI C'CwwG_G
Cuts at: 513 1065 2022 4550 513
Size: 552 957 2528 3074
Fragments arranged by size:
3074 2528 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2779 5138 5155 5308 5493 5938 325
Size: 1255 1199 2359 17 153 185 445 1498
Fragments arranged by size:
2359 1498 1255 1199 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2783 3137 3254
Size: 274 80 33 51 1443 806 354 117
Cuts at: 3254 3348 3531 3663 3755 4319 5357 7069 96
Size: 94 183 132 92 564 1038 1712 138
Fragments arranged by size:
1712 1443 1038 806 564 354 274 183
138 132 117 94 92 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7071 7103 7071
Size: 32 7079
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7111
XbaI T'CTAG_A
Cuts at: 2821 4447 2821
Size: 1626 5485
XhoI C'TCGA_G
Cuts at: 1943 3563 1943
Size: 1620 5491
XmaI C'CCGG_G
Cuts at: 1870 4436 1870
Size: 2566 4545
XmnI GAAnn'nnTTC
Cuts at: 2163 5240 2163
Size: 3077 4034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4919 276
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI