Restriction map for
pFN21ASDA0764
(Circular) (Six-base) MAPSORT of: pFN21ASDA0764.seq Check: 5817 from: 1 to: 5971
pFN21ASDA0764 5971 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2248 3781 278
Size: 53 83 186 936 712 1533 2468
Fragments arranged by size:
2468 1533 936 712 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2331 2805 4034 5418 5686 1632
Size: 699 474 1229 1384 268 1917
Fragments arranged by size:
1917 1384 1229 699 474 268
AfeI AGC'GCT
Cuts at: 3239 3239
Size: 5971
AflII C'TTAA_G
Cuts at: 820 1017 2594 820
Size: 197 1577 4197
Fragments arranged by size:
4197 1577 197
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AlwNI CAG_nnn'CTG
Cuts at: 2926 3714 5286 2926
Size: 788 1572 3611
Fragments arranged by size:
3611 1572 788
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 1870
Size: 73 1353 4545
Fragments arranged by size:
4545 1353 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 4747 618
Size: 325 217 627 1505 1455 1842
Fragments arranged by size:
1842 1505 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2950 3164 3290 1391
Size: 1559 214 126 4072
Fragments arranged by size:
4072 1559 214 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2240 3106 928
Size: 971 341 866 3793
Fragments arranged by size:
3793 971 866 341
BbvCI CC'TCA_GC
Cuts at: 2149 2149
Size: 5971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2465 2499 4162 4196 1903
Size: 34 528 34 1663 34 3678
Fragments arranged by size:
3678 1663 528 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2339 4032 5188 1790
Size: 549 1693 1156 2573
Fragments arranged by size:
2573 1693 1156 549
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 3702 4660
Size: 416 53 83 399 24 633 1652 958
Cuts at: 4660 442
Size: 1753
Fragments arranged by size:
1753 1652 958 633 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2340 2340
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 2699 2915 2934 2996 4631 1239
Size: 1340 120 216 19 62 1635 2579
Fragments arranged by size:
2579 1635 1340 216 120 62 19
Bpu10I CC'TnA_GC
Cuts at: 2149 2271 2149
Size: 122 5849
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3045 4096 4961 5259 3045
Size: 1051 865 298 3757
Fragments arranged by size:
3757 1051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2555 2910 4634 882
Size: 617 1056 355 1724 2219
Fragments arranged by size:
2219 1724 1056 617 355
BsaAI yAC'GTr
Cuts at: 493 1204 3268 3762 493
Size: 711 2064 494 2702
Fragments arranged by size:
2702 2064 711 494
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2245 3778 4160 4795
Size: 53 83 186 936 712 1533 382 635
Cuts at: 4795 275
Size: 1451
Fragments arranged by size:
1533 1451 936 712 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2337 2367 3113 3143 1178
Size: 30 165 30 934 30 746 30 4006
Fragments arranged by size:
4006 934 746 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2340 2483 2903 3015 3134 1186
Size: 1154 143 420 112 119 4023
Fragments arranged by size:
4023 1154 420 143 119 112
BseYI C'CCAG_C
Cuts at: 2641 3100 3146 5174 5670 2641
Size: 459 46 2028 496 2942
Fragments arranged by size:
2942 2028 496 459 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 3027 1163
Size: 573 387 904 4107
Fragments arranged by size:
4107 904 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2709 4032 4117 5188 2709
Size: 1323 85 1071 3492
Fragments arranged by size:
3492 1323 1071 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2339 2709 2950 3164 3290 4032 4117
Size: 399 549 370 241 214 126 742 85
Cuts at: 4117 5188 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 549 399 370 241 214
126 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2665 3326 844
Size: 1821 661 3489
Fragments arranged by size:
3489 1821 661
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3085 4466 4640 65
Size: 1183 1837 1381 174 1396
Fragments arranged by size:
1837 1396 1381 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 2332 4031 5043 2332
Size: 1699 1012 3260
Fragments arranged by size:
3260 1699 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3097 794
Size: 2303 3668
BstBI TT'CG_AA
Cuts at: 2007 4775 2007
Size: 2768 3203
BstEII G'GTnAC_C
Cuts at: 1184 2343 1184
Size: 1159 4812
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2518 2179
Size: 339 5632
BstYI r'GATC_y
Cuts at: 1972 2406 3301 3668 4053 4070 5522 5608 5620
Size: 434 895 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 895 434 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2099 513
Size: 552 1034 4385
Fragments arranged by size:
4385 1034 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 2707 2924 3112 3487 4281 4301 2511
Size: 196 217 188 375 794 20 4181
Fragments arranged by size:
4181 794 375 217 196 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2508 3418 4307
Size: 54 1425 50 275 18 678 910 889
Cuts at: 4307 5936 8
Size: 1629 43
Fragments arranged by size:
1629 1425 910 889 678 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 2331 3242 3901 1353
Size: 954 24 911 659 3423
Fragments arranged by size:
3423 954 911 659 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2331 2579 2699 2805 2915 2934 2996
Size: 393 699 248 120 106 110 19 62
Cuts at: 2996 4034 4631 5418 5686 1239
Size: 1038 597 787 268 1524
Fragments arranged by size:
1524 1038 787 699 597 393 268 248
120 110 106 62 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 3129 2498
Size: 631 5340
EcoO109I rG'GnC_Cy
Cuts at: 3216 3216
Size: 5971
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 3241 5118 1678
Size: 1563 1877 2531
Fragments arranged by size:
2531 1877 1563
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 2791 2823 3113
Size: 32 135 32 602 32 585 32 290
Cuts at: 3113 3145 4612 4644 4686 4718 1373
Size: 32 1467 32 42 32 2626
Fragments arranged by size:
2626 1467 602 585 290 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2985 748
Size: 2237 3734
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 10
Size: 54 1425 343 678 3471
Fragments arranged by size:
3471 1425 678 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3929 4288 4447 518
Size: 648 2763 359 159 2042
Fragments arranged by size:
2763 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2472 3387 4064 5212 5457 1606
Size: 866 915 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 866 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5419
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2761 3329 4874 1132
Size: 97 1532 568 1545 2229
Fragments arranged by size:
2229 1545 1532 568 97
PasI CC'CwG_GG
Cuts at: 1348 2339 1348
Size: 991 4980
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2506 2689 1108
Size: 1398 183 4390
Fragments arranged by size:
4390 1398 183
PfoI T'CCnGG_A
Cuts at: 2556 2556
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3216 3216
Size: 5971
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3323 830
Size: 2493 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2472 2472
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2762 3319 4454 5135 5326 826
Size: 219 1717 557 1135 681 191 1471
Fragments arranged by size:
1717 1471 1135 681 557 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2594 3060 4075 4976 5238 820
Size: 197 926 651 466 1015 901 262 1553
Fragments arranged by size:
1553 1015 926 901 651 466 262 197
SnaBI TAC'GTA
Cuts at: 493 3268 3762 493
Size: 2775 494 2702
Fragments arranged by size:
2775 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 3410 513
Size: 552 2345 3074
Fragments arranged by size:
3074 2345 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 2337 2369 5931 5963 2091
Size: 32 214 32 3562 32 2099
Fragments arranged by size:
3562 2099 214 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 2325 4100 2325
Size: 1775 4196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2246 3779 276
Size: 53 83 186 936 712 1533 2468
Fragments arranged by size:
2468 1533 936 712 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI