Restriction map for
pFN21ASDA0760
(Circular) (Six-base) MAPSORT of: pFN21ASDA0760.seq Check: 2974 from: 1 to: 8401
pFN21ASDA0760 8401 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2054 3981 4129 4326 4695 5251 2054
Size: 1927 148 197 369 556 5204
Fragments arranged by size:
5204 1927 556 369 197 148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5094 6211 278
Size: 53 83 186 936 3558 1117 2468
Fragments arranged by size:
3558 2468 1117 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2757 2856 3908 5744 1937
Size: 820 99 1052 1836 4594
Fragments arranged by size:
4594 1836 1052 820 99
Acc65I G'GTAC_C
Cuts at: 4375 5722 4375
Size: 1347 7054
AclI AA'CG_TT
Cuts at: 1563 4641 6528 6901 1563
Size: 3078 1887 373 3063
Fragments arranged by size:
3078 3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 2641 3149 3815 4195 6464 7848 8116
Size: 869 140 508 666 380 2269 1384 268
Cuts at: 8116 1632
Size: 1917
Fragments arranged by size:
2269 1917 1384 869 666 508 380 268
140
AfeI AGC'GCT
Cuts at: 4742 4742
Size: 8401
AflII C'TTAA_G
Cuts at: 820 1017 4430 820
Size: 197 3413 4791
Fragments arranged by size:
4791 3413 197
AflIII A'CryG_T
Cuts at: 1203 3167 4482 4719 7300 8277 1203
Size: 1964 1315 237 2581 977 1327
Fragments arranged by size:
2581 1964 1327 1315 977 237
AgeI A'CCGG_T
Cuts at: 7232 7232
Size: 8401
AhdI GACnn_n'nnGTC
Cuts at: 2071 7130 2071
Size: 5059 3342
AleI CACnn'nnGTG
Cuts at: 1166 3465 3992 4220 1166
Size: 2299 527 228 5347
Fragments arranged by size:
5347 2299 527 228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2264 5604 1184
Size: 1080 3340 3981
Fragments arranged by size:
3981 3340 1080
AlwNI CAG_nnn'CTG
Cuts at: 3883 4978 5020 5077 6144 7716 3883
Size: 1095 42 57 1067 1572 4568
Fragments arranged by size:
4568 1572 1095 1067 57 42
ApaI G_GGCC'C
Cuts at: 2104 4706 5301 2104
Size: 2602 595 5204
Fragments arranged by size:
5204 2602 595
ApaLI G'TGCA_C
Cuts at: 2344 2512 4052 4142 5594 6458 7614 2344
Size: 168 1540 90 1452 864 1156 3131
Fragments arranged by size:
3131 1540 1452 1156 864 168 90
ApoI r'AATT_y
Cuts at: 1493 5710 5936 6088 8197 1493
Size: 4217 226 152 2109 1697
Fragments arranged by size:
4217 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6955 160
Size: 872 5923 1606
Fragments arranged by size:
5923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8401
AvaI C'yCGr_G
Cuts at: 1870 1943 3299 5171 5210 5726 1870
Size: 73 1356 1872 39 516 4545
Fragments arranged by size:
4545 1872 1356 516 73 39
BamHI G'GATC_C
Cuts at: 5731 6098 5731
Size: 367 8034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4375 4444 4805 4958 5309
Size: 325 217 627 2588 69 361 153 351
Cuts at: 5309 5522 5722 7177 618
Size: 213 200 1455 1842
Fragments arranged by size:
2588 1842 1455 627 361 351 325 217
213 200 153 69
BanII G_rGCy'C
Cuts at: 1391 2104 3656 4494 4706 4947 5178 5301 5720
Size: 713 1552 838 212 241 231 123 419
Cuts at: 5720 1391
Size: 4072
Fragments arranged by size:
4072 1552 838 713 419 241 231 212
123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2489 2743 3137 3672 4018 5247 5545
Size: 971 590 254 394 535 346 1229 298
Cuts at: 5545 928
Size: 3784
Fragments arranged by size:
3784 1229 971 590 535 394 346 298
254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6592 6626 1903
Size: 34 4655 34 3678
Fragments arranged by size:
4655 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3747 6293 7503 3747
Size: 2546 1210 4645
Fragments arranged by size:
4645 2546 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4164 7012 136
Size: 107 122 71 631 605 2492 2848 1525
Fragments arranged by size:
2848 2492 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3174 5613 8396 3174
Size: 2439 2783 3179
Fragments arranged by size:
3179 2783 2439
BlpI GC'TnA_GC
Cuts at: 4401 4990 5818 4401
Size: 589 828 6984
Fragments arranged by size:
6984 828 589
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2243 2348 2516 2819 2828 3836 4056
Size: 314 139 105 168 303 9 1008 220
Cuts at: 4056 4146 4706 4808 5301 5331 5598 5636 6462
Size: 90 560 102 493 30 267 38 826
Cuts at: 6462 7618 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1008 826 560 493 314 303
267 220 168 139 105 102 90 38
30 9
BmgBI CAC'GTC
Cuts at: 1318 3906 4147 4477 1318
Size: 2588 241 330 5242
Fragments arranged by size:
5242 2588 330 241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2933 3650 3832
Size: 416 53 83 399 24 1516 717 182
Cuts at: 3832 4120 6132 7090 442
Size: 288 2012 958 1753
Fragments arranged by size:
2012 1753 1516 958 717 416 399 288
182 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8401
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 3001 2854
Size: 147 8254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2149 2479 2696 2875 2947 3137 3827
Size: 897 13 330 217 179 72 190 690
Cuts at: 3827 4328 5267 7061 1239
Size: 501 939 1794 2579
Fragments arranged by size:
2579 1794 939 897 690 501 330 217
190 179 72 13
Bpu10I CC'TnA_GC
Cuts at: 2829 2829
Size: 8401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2311 2453 3743 4931 4961 5036 6526 7391 7689
Size: 142 1290 1188 30 75 1490 865 298
Cuts at: 7689 2311
Size: 3023
Fragments arranged by size:
3023 1490 1290 1188 865 298 142 75
30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4199 5282 7064 882
Size: 617 2700 1083 1782 2219
Fragments arranged by size:
2700 2219 1782 1083 617
BsaAI yAC'GTr
Cuts at: 493 1204 2686 5698 6192 493
Size: 711 1482 3012 494 2702
Fragments arranged by size:
3012 2702 1482 711 494
BsaBI GATnn'nnATC
Cuts at: 5393 8395 5393
Size: 3002 5399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4997 5091 6208 6590
Size: 53 83 186 936 3464 94 1117 382
Cuts at: 6590 7225 275
Size: 635 1451
Fragments arranged by size:
3464 1451 1117 936 635 382 186 94
83 53
BsaWI w'CCGG_w
Cuts at: 3493 6834 7232 7506 7653 8064 3493
Size: 3341 398 274 147 411 3830
Fragments arranged by size:
3830 3341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3392 3422 3707 3737 3842
Size: 30 165 30 1989 30 285 30 105
Cuts at: 3842 3872 4217 4247 4481 4511 1178
Size: 30 345 30 234 30 5068
Fragments arranged by size:
5068 1989 345 285 234 165 105 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2392 2527 2684 2830 4856 4859 5471
Size: 1057 149 135 157 146 2026 3 612
Cuts at: 5471 1186
Size: 4116
Fragments arranged by size:
4116 2026 1057 612 157 149 146 135
3
BseYI C'CCAG_C
Cuts at: 3153 7604 8100 3153
Size: 4451 496 3454
Fragments arranged by size:
4451 3454 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3215 3521 3529 3785 3991 4081 5236
Size: 573 1479 306 8 256 206 90 1155
Cuts at: 5236 1163
Size: 4328
Fragments arranged by size:
4328 1479 1155 573 306 256 206 90
8
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2389 4353 4580 5185 5851 6612
Size: 275 186 388 1964 227 605 666 761
Cuts at: 6612 7640 1540
Size: 1028 2301
Fragments arranged by size:
2301 1964 1028 761 666 605 388 275
227 186
BsiHKAI G_wGCw'C
Cuts at: 2348 2516 2691 3219 3336 3410 3447 4056 4146
Size: 168 175 528 117 74 37 609 90
Cuts at: 4146 4686 4860 4947 5502 5598 6462 6547 7618
Size: 540 174 87 555 96 864 85 1071
Cuts at: 7618 2348
Size: 3131
Fragments arranged by size:
3131 1071 864 609 555 540 528 175
174 168 117 96 90 87 85 74
37
BsmI GAATG_Cn'
Cuts at: 2733 5912 6005 2733
Size: 3179 93 5129
Fragments arranged by size:
5129 3179 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2163 3349 2163
Size: 1186 7215
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2243 2348 2516 2691 2819 2828
Size: 399 314 139 105 168 175 128 9
Cuts at: 2828 3219 3336 3410 3447 3656 3836 4056 4146
Size: 391 117 74 37 209 180 220 90
Cuts at: 4146 4494 4686 4706 4808 4860 4947 5178 5301
Size: 348 192 20 102 52 87 231 123
Cuts at: 5301 5331 5502 5598 5636 5720 6462 6547 7618
Size: 30 171 96 38 84 742 85 1071
Cuts at: 7618 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 391 348 314 231
220 209 192 180 175 171 168 139
128 123 117 105 102 96 90 87
85 84 74 52 38 37 30 20
9
BspEI T'CCGG_A
Cuts at: 8064 8064
Size: 8401
BspHI T'CATG_A
Cuts at: 6290 8020 6290
Size: 1730 6671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 2733 3969 3981 4129 4143 4326 4422
Size: 1210 679 1236 12 148 14 183 96
Cuts at: 4422 4695 4887 5251 5756 844
Size: 273 192 364 505 3489
Fragments arranged by size:
3489 1236 1210 679 505 364 273 192
183 148 96 14 12
BsrBI CCG'CTC
Cuts at: 1394 1823 6288 1394
Size: 429 4465 3507
Fragments arranged by size:
4465 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4081 6896 7070 65
Size: 1183 2833 2815 174 1396
Fragments arranged by size:
2833 2815 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2388 2877 4554 5120 5184 5195 7045
Size: 110 578 489 1677 566 64 11 1850
Cuts at: 7045 7232 1700
Size: 187 2869
Fragments arranged by size:
2869 1850 1677 578 566 489 187 110
64 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8401
BssSI C'ACGA_G
Cuts at: 2309 2432 5435 6461 7473 2309
Size: 123 3003 1026 1012 3237
Fragments arranged by size:
3237 3003 1026 1012 123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2352 2520 2739 3294 3405 3750 4104 4423
Size: 1558 168 219 555 111 345 354 319
Cuts at: 4423 5358 794
Size: 935 3837
Fragments arranged by size:
3837 1558 935 555 354 345 319 219
168 111
BstBI TT'CG_AA
Cuts at: 2007 7205 2007
Size: 5198 3203
BstEII G'GTnAC_C
Cuts at: 1184 4777 1184
Size: 3593 4808
BstYI r'GATC_y
Cuts at: 1972 2316 2472 3120 3174 4950 5388 5613 5731
Size: 344 156 648 54 1776 438 225 118
Cuts at: 5731 6098 6483 6500 7952 8038 8050 8396 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1776 1452 648 438 385 367 346
344 225 156 118 86 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 2745 4440 2745
Size: 1695 6706
BtgI C'CryG_G
Cuts at: 513 1065 2022 3411 4530 4954 513
Size: 552 957 1389 1119 424 3960
Fragments arranged by size:
3960 1389 1119 957 552 424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5304 487
Size: 683 56 4078 3584
Fragments arranged by size:
4078 3584 683 56
BtsI GCAGTG_nn'
Cuts at: 2425 2735 2737 2768 3283 3349 3460 3472 3481
Size: 310 2 31 515 66 111 12 9
Cuts at: 3481 4003 4238 4858 5224 5917 6711 6731 2425
Size: 522 235 620 366 693 794 20 4095
Fragments arranged by size:
4095 794 693 620 522 515 366 310
235 111 66 31 20 12 9 2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8366
DraI TTT'AAA
Cuts at: 5705 5705
Size: 8401
DraIII CAC_nnn'GTG
Cuts at: 2520 3919 4014 4336 4777 2520
Size: 1399 95 322 441 6144
Fragments arranged by size:
6144 1399 441 322 95
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7408 809
Size: 507 6092 1802
Fragments arranged by size:
6092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2595 3108 4173
Size: 54 1425 50 275 18 765 513 1065
Cuts at: 4173 4365 4386 4577 5011 5472 5848 6737 8366
Size: 192 21 191 434 461 376 889 1629
Cuts at: 8366 8
Size: 43
Fragments arranged by size:
1629 1425 1065 889 765 513 461 434
376 275 192 191 54 50 43 21
18
EagI C'GGCC_G
Cuts at: 1537 1812 4577 5848 1537
Size: 275 2765 1271 4090
Fragments arranged by size:
4090 2765 1271 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2297 2369 6331 1353
Size: 944 72 3962 3423
Fragments arranged by size:
3962 3423 944 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2735 6988 7362 7508 387
Size: 1064 138 1146 4253 374 146 1280
Fragments arranged by size:
4253 1280 1146 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4945 4945
Size: 8401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2149 2479 2501 2641 2696 2875
Size: 393 504 13 330 22 140 55 179
Cuts at: 2875 2947 3137 3149 3815 3827 4195 4328 5267
Size: 72 190 12 666 12 368 133 939
Cuts at: 5267 6464 7061 7848 8116 1239
Size: 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 939 787 666 597 504 393
368 330 268 190 179 140 133 72
55 22 13 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 4016 2225
Size: 1791 6610
EcoO109I rG'GnC_Cy
Cuts at: 4702 4703 5074 5298 4702
Size: 1 371 224 7805
Fragments arranged by size:
7805 371 224 1
EcoRI G'AATT_C
Cuts at: 5710 6088 5710
Size: 378 8023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2644 3112 4210 5665 2548
Size: 96 468 1098 1455 5284
Fragments arranged by size:
5284 1455 1098 468 96
FspI TGC'GCA
Cuts at: 4883 6907 4883
Size: 2024 6377
HaeII r_GCGC'y
Cuts at: 1678 2952 2970 3714 4744 5142 7548 1678
Size: 1274 18 744 1030 398 2406 2531
Fragments arranged by size:
2531 2406 1274 1030 744 398 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2057 2089 2165
Size: 32 135 32 446 32 7 32 76
Cuts at: 2165 2197 3842 3874 4580 4612 7042 7074 7116
Size: 32 1645 32 706 32 2430 32 42
Cuts at: 7116 7148 1373
Size: 32 2626
Fragments arranged by size:
2626 2430 1645 706 446 135 76 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2758 3909 5745 5991 669
Size: 988 281 820 1151 1836 246 3079
Fragments arranged by size:
3079 1836 1151 988 820 281 246
HindIII A'AGCT_T
Cuts at: 748 5084 748
Size: 4336 4065
HpaI GTT'AAC
Cuts at: 5991 5991
Size: 8401
KpnI G_GTAC'C
Cuts at: 4379 5726 4379
Size: 1347 7054
MfeI C'AATT_G
Cuts at: 6000 6000
Size: 8401
MluI A'CGCG_T
Cuts at: 4482 8277 4482
Size: 3795 4606
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2583 3792 4467 7515 7699 1305
Size: 1278 1209 675 3048 184 2007
Fragments arranged by size:
3048 2007 1278 1209 675 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2597 4388 5013 10
Size: 54 1425 343 765 1791 625 3398
Fragments arranged by size:
3398 1791 1425 765 625 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 3465 3992 4049 4074 4220 4535
Size: 648 1026 1273 527 57 25 146 315
Cuts at: 4535 5261 5347 6359 6718 6877 518
Size: 726 86 1012 359 159 2042
Fragments arranged by size:
2042 1273 1026 1012 726 648 527 359
315 159 146 86 57 25
MspA1I CmG'CkG
Cuts at: 1606 2109 2707 3013 3697 4095 4905 4994 5817
Size: 503 598 306 684 398 810 89 823
Cuts at: 5817 6494 7642 7887 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 810 684 677 598 503
398 306 245 89
NaeI GCC'GGC
Cuts at: 5122 5197 5122
Size: 75 8326
NcoI C'CATG_G
Cuts at: 513 1065 2022 3411 4530 4954 513
Size: 552 957 1389 1119 424 3960
Fragments arranged by size:
3960 1389 1119 957 552 424
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8401
NgoMIV G'CCGG_C
Cuts at: 5120 5195 5120
Size: 75 8326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8401
NotI GC'GGCC_GC
Cuts at: 5848 5848
Size: 8401
NspI r_CATG'y
Cuts at: 1132 1229 2566 3171 3985 4426 4723 4921 4972
Size: 97 1337 605 814 441 297 198 51
Cuts at: 4972 5260 5759 7304 1132
Size: 288 499 1545 2229
Fragments arranged by size:
2229 1545 1337 814 605 499 441 297
288 198 97 51
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8401
PciI A'CATG_T
Cuts at: 3167 4719 7300 3167
Size: 1552 2581 4268
Fragments arranged by size:
4268 2581 1552
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8401
PfoI T'CCnGG_A
Cuts at: 2113 2113
Size: 8401
PmeI GTTT'AAAC
Cuts at: 5705 5705
Size: 8401
PmlI CAC'GTG
Cuts at: 2686 2686
Size: 8401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6470 6502 7983 8015 6470
Size: 32 1481 32 6856
Fragments arranged by size:
6856 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5074 5074
Size: 8401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8401
PsiI TTA'TAA
Cuts at: 5971 5971
Size: 8401
PspOMI G'GGCC_C
Cuts at: 2100 4702 5297 2100
Size: 2602 595 5204
Fragments arranged by size:
5204 2602 595
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 8369
PstI C_TGCA'G
Cuts at: 830 2357 2556 2730 3330 3467 3879 3966 4140
Size: 1527 199 174 600 137 412 87 174
Cuts at: 4140 4233 4280 4419 4548 4692 4836 5025 5667
Size: 93 47 139 129 144 144 189 642
Cuts at: 5667 5753 830
Size: 86 3478
Fragments arranged by size:
3478 1527 642 600 412 199 189 174
174 144 144 139 137 129 93 87
86 47
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8401
PvuII CAG'CTG
Cuts at: 2707 2707
Size: 8401
SacI G_AGCT'C
Cuts at: 4947 4947
Size: 8401
SalI G'TCGA_C
Cuts at: 1936 2756 3907 5743 1936
Size: 820 1151 1836 4594
Fragments arranged by size:
4594 1836 1151 820
SbfI CC_TGCA'GG
Cuts at: 3330 4140 4419 5753 3330
Size: 810 279 1334 5978
Fragments arranged by size:
5978 1334 810 279
ScaI AGT'ACT
Cuts at: 6649 8361 6649
Size: 1712 6689
SexAI A'CCwGG_T
Cuts at: 5076 5076
Size: 8401
SfcI C'TryA_G
Cuts at: 826 1045 2353 2552 2726 3067 3326 3463 3875
Size: 219 1308 199 174 341 259 137 412
Cuts at: 3875 3962 4136 4229 4276 4415 4544 4688 4832
Size: 87 174 93 47 139 129 144 144
Cuts at: 4832 5021 5663 5749 6884 7565 7756 826
Size: 189 642 86 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 681 642 412 341 259
219 199 191 189 174 174 144 144
139 137 129 93 87 86 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8401
SmaI CCC'GGG
Cuts at: 1872 5728 1872
Size: 3856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2290 2468 3758 4430 4940 4946
Size: 197 926 347 178 1290 672 510 6
Cuts at: 4946 5051 6505 7406 7668 820
Size: 105 1454 901 262 1553
Fragments arranged by size:
1553 1454 1290 926 901 672 510 347
262 197 178 105 6
SnaBI TAC'GTA
Cuts at: 493 5698 6192 493
Size: 5205 494 2702
Fragments arranged by size:
5205 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8401
SphI G_CATG'C
Cuts at: 1132 4426 4921 5759 1132
Size: 3294 495 838 3774
Fragments arranged by size:
3774 3294 838 495
SspI AAT'ATT
Cuts at: 5 52 6325 5
Size: 47 6273 2081
Fragments arranged by size:
6273 2081 47
StuI AGG'CCT
Cuts at: 1517 4286 1517
Size: 2769 5632
StyI C'CwwG_G
Cuts at: 513 1065 2022 3057 3411 3639 4035 4176 4530
Size: 552 957 1035 354 228 396 141 354
Cuts at: 4530 4954 5840 513
Size: 424 886 3074
Fragments arranged by size:
3074 1035 957 886 552 424 396 354
354 228 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 2200 4367 5144 5327 5672 6428
Size: 1255 557 63 2167 777 183 345 756
Cuts at: 6428 6445 6598 6783 7228 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2167 1498 1255 777 756 557 445 345
185 183 153 63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2920 6647 8359
Size: 274 80 33 51 1443 943 3727 1712
Cuts at: 8359 96
Size: 138
Fragments arranged by size:
3727 1712 1443 943 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2714 2746 4217 4249 8361 8393 2714
Size: 32 1471 32 4112 32 2722
Fragments arranged by size:
4112 2722 1471 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3904 1316
Size: 2588 5813
XbaI T'CTAG_A
Cuts at: 5737 5737
Size: 8401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2142 2971 4303 5149 2111
Size: 31 829 1332 846 5363
Fragments arranged by size:
5363 1332 846 829 31
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8401
XmaI C'CCGG_G
Cuts at: 1870 5726 1870
Size: 3856 4545
XmnI GAAnn'nnTTC
Cuts at: 5645 6530 5645
Size: 885 7516
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5092 6209 276
Size: 53 83 186 936 3558 1117 2468
Fragments arranged by size:
3558 2468 1117 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI