Restriction map for
pFN21ASDA0759
(Circular) (Six-base) MAPSORT of: pFN21ASDA0759.seq Check: 9684 from: 1 to: 6739
pFN21ASDA0759 6739 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3749 4549 278
Size: 53 83 186 936 2213 800 2468
Fragments arranged by size:
2468 2213 936 800 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4082 1937
Size: 2145 4594
Acc65I G'GTAC_C
Cuts at: 3719 4060 3719
Size: 341 6398
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2319 2747 3954 3966 4802 6186 6454 1632
Size: 687 428 1207 12 836 1384 268 1917
Fragments arranged by size:
1917 1384 1207 836 687 428 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 2099 5638 6615 1203
Size: 896 3539 977 1327
Fragments arranged by size:
3539 1327 977 896
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 2347 5468 2347
Size: 3121 3618
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6739
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2149 2117
Size: 32 6707
AlwNI CAG_nnn'CTG
Cuts at: 2725 2791 3297 4482 6054 2725
Size: 66 506 1185 1572 3410
Fragments arranged by size:
3410 1572 1185 506 66
ApaI G_GGCC'C
Cuts at: 2177 2262 2177
Size: 85 6654
ApaLI G'TGCA_C
Cuts at: 4796 5952 4796
Size: 1156 5583
ApoI r'AATT_y
Cuts at: 1493 2673 2876 3325 4048 4274 4426 6535 1493
Size: 1180 203 449 723 226 152 2109 1697
Fragments arranged by size:
2109 1697 1180 723 449 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 5293 160
Size: 872 4261 1606
Fragments arranged by size:
4261 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 4064 1870
Size: 73 226 1895 4545
Fragments arranged by size:
4545 1895 226 73
BamHI G'GATC_C
Cuts at: 4069 4436 4069
Size: 367 6372
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2522 3719 4060 5515 618
Size: 325 217 627 735 1197 341 1455 1842
Fragments arranged by size:
1842 1455 1197 735 627 341 325 217
BanII G_rGCy'C
Cuts at: 1391 2177 2262 2456 2490 2807 2814 3375 4058
Size: 786 85 194 34 317 7 561 683
Cuts at: 4058 1391
Size: 4072
Fragments arranged by size:
4072 786 683 561 317 194 85 34
7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3423 928
Size: 971 1524 4244
Fragments arranged by size:
4244 1524 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2999 3033 4930 4964 1903
Size: 34 1062 34 1897 34 3678
Fragments arranged by size:
3678 1897 1062 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4631 5841 4631
Size: 1210 5529
BclI T'GATC_A
Cuts at: 1548 3874 1548
Size: 2326 4413
BfrBI ATG'CAT
Cuts at: 3683 3683
Size: 6739
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3242 5350 136
Size: 107 122 71 631 605 1570 2108 1525
Fragments arranged by size:
2108 1570 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6734 6734
Size: 6739
BlpI GC'TnA_GC
Cuts at: 4156 4156
Size: 6739
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2262 2527 2595 4800 5956 1790
Size: 387 85 265 68 2205 1156 2573
Fragments arranged by size:
2573 2205 1156 387 265 85 68
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6739
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2485 2510 2896
Size: 416 53 83 399 24 1068 25 386
Cuts at: 2896 2944 3006 4470 5428 442
Size: 48 62 1464 958 1753
Fragments arranged by size:
1753 1464 1068 958 416 399 386 83
62 53 48 25 24
BmtI G_CTAG'C
Cuts at: 1056 3063 4004 1056
Size: 2007 941 3791
Fragments arranged by size:
3791 2007 941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 6739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 2453 2590 2894 3359 3691 5399 1239
Size: 1098 116 137 304 465 332 1708 2579
Fragments arranged by size:
2579 1708 1098 465 332 304 137 116
Bpu10I CC'TnA_GC
Cuts at: 2178 2583 2718 2784 2178
Size: 405 135 66 6133
Fragments arranged by size:
6133 405 135 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3692 3713 3887 4864 5729 6027 3692
Size: 21 174 977 865 298 4404
Fragments arranged by size:
4404 977 865 298 174 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5402 882
Size: 617 3903 2219
Fragments arranged by size:
3903 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4036 4530 493
Size: 711 2832 494 2702
Fragments arranged by size:
2832 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6733 6733
Size: 6739
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3746 4546 4928 5563
Size: 53 83 186 936 2213 800 382 635
Cuts at: 5563 275
Size: 1451
Fragments arranged by size:
2213 1451 936 800 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5172 5570 5844 5991 6402 5172
Size: 398 274 147 411 5509
Fragments arranged by size:
5509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2477 2507 1178
Size: 30 165 30 1074 30 5410
Fragments arranged by size:
5410 1074 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 3004 1186
Size: 1249 569 4921
Fragments arranged by size:
4921 1249 569
BseYI C'CCAG_C
Cuts at: 2554 2706 3467 3771 5942 6438 2554
Size: 152 761 304 2171 496 2855
Fragments arranged by size:
2855 2171 761 496 304 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6166
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2241 2807 2814 3375 4800 4885 5956 2241
Size: 566 7 561 1425 85 1071 3024
Fragments arranged by size:
3024 1425 1071 566 561 85 7
BsmI GAATG_Cn'
Cuts at: 2332 4250 4343 2332
Size: 1918 93 4728
Fragments arranged by size:
4728 1918 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3526 3923 3526
Size: 397 6342
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2241 2262 2456 2490 2527 2595
Size: 399 387 64 21 194 34 37 68
Cuts at: 2595 2807 2814 3375 4058 4800 4885 5956 1391
Size: 212 7 561 683 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 683 561 399 387 212
194 85 68 64 37 34 21 7
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 2668 4628 6358 2668
Size: 1960 1730 3049
Fragments arranged by size:
3049 1960 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 3655 4094 844
Size: 1211 1600 439 3489
Fragments arranged by size:
3489 1600 1211 439
BsrBI CCG'CTC
Cuts at: 1394 1823 4626 1394
Size: 429 2803 3507
Fragments arranged by size:
3507 2803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2821 5234 5408 65
Size: 1183 1573 2413 174 1396
Fragments arranged by size:
2413 1573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 5383 5570 1700
Size: 110 246 3327 187 2869
Fragments arranged by size:
3327 2869 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 4799 5811 4799
Size: 1012 5727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2504 3297 794
Size: 1710 793 4236
Fragments arranged by size:
4236 1710 793
BstBI TT'CG_AA
Cuts at: 2007 5543 2007
Size: 3536 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstXI CCAn_nnnn'nTGG
Cuts at: 3253 3383 3475 3253
Size: 130 92 6517
Fragments arranged by size:
6517 130 92
BstYI r'GATC_y
Cuts at: 1972 4069 4436 4821 4838 6290 6376 6388 6734
Size: 2097 367 385 17 1452 86 12 346
Cuts at: 6734 1972
Size: 1977
Fragments arranged by size:
2097 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2160 3382 513
Size: 552 1095 1222 3870
Fragments arranged by size:
3870 1222 1095 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3237 3565 487
Size: 683 56 2011 328 3661
Fragments arranged by size:
3661 2011 683 328 56
BtsI GCAGTG_nn'
Cuts at: 2216 2426 2546 2658 3295 3730 4255 5049 5069
Size: 210 120 112 637 435 525 794 20
Cuts at: 5069 2216
Size: 3886
Fragments arranged by size:
3886 794 637 525 435 210 120 112
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 4043 4043
Size: 6739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5746 809
Size: 507 4430 1802
Fragments arranged by size:
4430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2529 3243
Size: 54 1425 50 275 18 228 471 714
Cuts at: 3243 3273 3544 3778 4186 5075 6704 8
Size: 30 271 234 408 889 1629 43
Fragments arranged by size:
1629 1425 889 714 471 408 275 271
234 228 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2316 2541 2595 3930 3985 4669 1353
Size: 722 241 225 54 1335 55 684 3423
Fragments arranged by size:
3423 1335 722 684 241 225 55 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3035 3272 5326 5700 5846 387
Size: 1064 138 1446 237 2054 374 146 1280
Fragments arranged by size:
2054 1446 1280 1064 374 237 146 138
EcoICRI GAG'CTC
Cuts at: 2805 2812 3373 2805
Size: 7 561 6171
Fragments arranged by size:
6171 561 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 2337 2453 2590 2747 2894 3359
Size: 393 687 18 116 137 157 147 465
Cuts at: 3359 3691 3954 3966 4802 5399 6186 6454 1239
Size: 332 263 12 836 597 787 268 1524
Fragments arranged by size:
1524 836 787 687 597 465 393 332
268 263 157 147 137 116 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2783 2870 3706 2783
Size: 87 836 5816
Fragments arranged by size:
5816 836 87
EcoO109I rG'GnC_Cy
Cuts at: 2173 2174 2197 2258 2259 2551 2626 2722 2788
Size: 1 23 61 1 292 75 96 66
Cuts at: 2788 2890 3319 3454 2173
Size: 102 429 135 5458
Fragments arranged by size:
5458 429 292 135 102 96 75 66
61 23 1 1
EcoRI G'AATT_C
Cuts at: 2876 4048 4426 2876
Size: 1172 378 5189
Fragments arranged by size:
5189 1172 378
EcoRV GAT'ATC
Cuts at: 2899 2899
Size: 6739
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 5886 1678
Size: 4208 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 2333 2365 5380
Size: 32 135 32 724 32 5 32 3015
Cuts at: 5380 5412 5454 5486 1373
Size: 32 42 32 2626
Fragments arranged by size:
3015 2626 724 135 42 32 32 32
32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 4083 4329 669
Size: 988 281 2145 246 3079
Fragments arranged by size:
3079 2145 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6739
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 3723 4064 3723
Size: 341 6398
MfeI C'AATT_G
Cuts at: 2842 4338 2842
Size: 1496 5243
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2463 5853 6037 1305
Size: 1158 3390 184 2007
Fragments arranged by size:
3390 2007 1158 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2531 3245 3275 10
Size: 54 1425 343 699 714 30 3474
Fragments arranged by size:
3474 1425 714 699 343 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2762 3797 4697 5056 5215 518
Size: 648 1596 1035 900 359 159 2042
Fragments arranged by size:
2042 1596 1035 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 2558 2944 3178 3845 4155 4832 5980
Size: 756 196 386 234 667 310 677 1148
Cuts at: 5980 6225 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 756 677 667 386 310 245
234 196
NaeI GCC'GGC
Cuts at: 2058 2058
Size: 6739
NcoI C'CATG_G
Cuts at: 513 1065 2160 3382 513
Size: 552 1095 1222 3870
Fragments arranged by size:
3870 1222 1095 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6739
NgoMIV G'CCGG_C
Cuts at: 2056 2056
Size: 6739
NheI G'CTAG_C
Cuts at: 1052 3059 4000 1052
Size: 2007 941 3791
Fragments arranged by size:
3791 2007 941
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NsiI A_TGCA'T
Cuts at: 3685 3685
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 2103 4097 5642 1132
Size: 97 874 1994 1545 2229
Fragments arranged by size:
2229 1994 1545 874 97
PasI CC'CwG_GG
Cuts at: 1348 3475 1348
Size: 2127 4612
PciI A'CATG_T
Cuts at: 2099 5638 2099
Size: 3539 3200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6739
PfoI T'CCnGG_A
Cuts at: 2314 2910 2919 3707 2314
Size: 596 9 788 5346
Fragments arranged by size:
5346 788 596 9
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2551 2788 2890 3319 2551
Size: 237 102 429 5971
Fragments arranged by size:
5971 429 237 102
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6739
PsiI TTA'TAA
Cuts at: 2772 4309 2772
Size: 1537 5202
PspOMI G'GGCC_C
Cuts at: 2173 2258 2173
Size: 85 6654
PstI C_TGCA'G
Cuts at: 830 2208 2383 3453 3578 4091 830
Size: 1378 175 1070 125 513 3478
Fragments arranged by size:
3478 1378 1070 513 175 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
PvuII CAG'CTG
Cuts at: 2362 2944 3845 2362
Size: 582 901 5256
Fragments arranged by size:
5256 901 582
SacI G_AGCT'C
Cuts at: 2807 2814 3375 2807
Size: 7 561 6171
Fragments arranged by size:
6171 561 7
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SanDI GG'GwC_CC
Cuts at: 2890 2890
Size: 6739
SapI GCTCTTCn'nnn_
Cuts at: 3985 3985
Size: 6739
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 3074 4987 6699 3074
Size: 1913 1712 3114
Fragments arranged by size:
3114 1913 1712
SfcI C'TryA_G
Cuts at: 826 1045 2204 2379 3449 3574 3714 4087 5222
Size: 219 1159 175 1070 125 140 373 1135
Cuts at: 5222 5903 6094 826
Size: 681 191 1471
Fragments arranged by size:
1471 1159 1135 1070 681 373 219 191
175 140 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 3242 3242
Size: 6739
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3671 3692 3902 4843 5744 6006
Size: 197 926 1728 21 210 941 901 262
Cuts at: 6006 820
Size: 1553
Fragments arranged by size:
1728 1553 941 926 901 262 210 197
21
SnaBI TAC'GTA
Cuts at: 493 4036 4530 493
Size: 3543 494 2702
Fragments arranged by size:
3543 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 4097 1132
Size: 2965 3774
SspI AAT'ATT
Cuts at: 5 52 4663 5
Size: 47 4611 2081
Fragments arranged by size:
4611 2081 47
StuI AGG'CCT
Cuts at: 1517 3153 1517
Size: 1636 5103
StyI C'CwwG_G
Cuts at: 513 1065 2160 3382 4178 513
Size: 552 1095 1222 796 3074
Fragments arranged by size:
3074 1222 1095 796 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2510 2720 2988 4766 4783 4936 5121
Size: 1255 930 210 268 1778 17 153 185
Cuts at: 5121 5566 325
Size: 445 1498
Fragments arranged by size:
1778 1498 1255 930 445 268 210 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3072 4985 6697
Size: 274 80 33 51 1443 1095 1913 1712
Cuts at: 6697 96
Size: 138
Fragments arranged by size:
1913 1712 1443 1095 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6699 6731 6699
Size: 32 6707
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 4075 4075
Size: 6739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4012 4012
Size: 6739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 4868 4868
Size: 6739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3747 4547 276
Size: 53 83 186 936 2213 800 2468
Fragments arranged by size:
2468 2213 936 800 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI
BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI
NruI PacI PmlI PspXI PsrI RsrII SacII SexAI
SfoI SrfI SwaI