Restriction map for
pFN21ASDA0755
(Circular) (Six-base) MAPSORT of: pFN21ASDA0755.seq Check: 7395 from: 1 to: 7825
pFN21ASDA0755 7825 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3464 5635 278
Size: 53 83 186 936 1928 2171 2468
Fragments arranged by size:
2468 2171 1928 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4795 5168 1937
Size: 2858 373 4594
Fragments arranged by size:
4594 2858 373
Acc65I G'GTAC_C
Cuts at: 5146 5146
Size: 7825
AclI AA'CG_TT
Cuts at: 1563 5952 6325 1563
Size: 4389 373 3063
Fragments arranged by size:
4389 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 3804 4923 5888 7272 7540 1632
Size: 1805 367 1119 965 1384 268 1917
Fragments arranged by size:
1917 1805 1384 1119 965 367 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7628
AflIII A'CryG_T
Cuts at: 1203 3129 3657 4701 4800 6724 7701 1203
Size: 1926 528 1044 99 1924 977 1327
Fragments arranged by size:
1926 1924 1327 1044 977 528 99
AgeI A'CCGG_T
Cuts at: 6656 6656
Size: 7825
AhdI GACnn_n'nnGTC
Cuts at: 4637 6554 4637
Size: 1917 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7825
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 2514
Size: 32 7793
AlwNI CAG_nnn'CTG
Cuts at: 5032 5568 7140 5032
Size: 536 1572 5717
Fragments arranged by size:
5717 1572 536
ApaLI G'TGCA_C
Cuts at: 5882 7038 5882
Size: 1156 6669
ApoI r'AATT_y
Cuts at: 1493 3435 4443 5134 5360 5512 7621 1493
Size: 1942 1008 691 226 152 2109 1697
Fragments arranged by size:
2109 1942 1697 1008 691 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6379 160
Size: 872 5347 1606
Fragments arranged by size:
5347 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
AvaI C'yCGr_G
Cuts at: 1870 1943 5150 1870
Size: 73 3207 4545
Fragments arranged by size:
4545 3207 73
BamHI G'GATC_C
Cuts at: 2113 2800 5155 5522 2113
Size: 687 2355 367 4416
Fragments arranged by size:
4416 2355 687 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2567 5146 6601 618
Size: 325 217 627 780 2579 1455 1842
Fragments arranged by size:
2579 1842 1455 780 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2245 2610 5144 1391
Size: 854 365 2534 4072
Fragments arranged by size:
4072 2534 854 365
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3250 3425 4245 4303 5046 928
Size: 971 1351 175 820 58 743 3707
Fragments arranged by size:
3707 1351 971 820 743 175 58
BbvCI CC'TCA_GC
Cuts at: 2067 3975 2067
Size: 1908 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6016 6050 1903
Size: 34 4079 34 3678
Fragments arranged by size:
4079 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5717 6927 5717
Size: 1210 6615
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7825
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2158 6436 136
Size: 107 122 71 631 605 486 4278 1525
Fragments arranged by size:
4278 1525 631 605 486 122 107 71
BglII A'GATC_T
Cuts at: 4602 5100 7820 4602
Size: 498 2720 4607
Fragments arranged by size:
4607 2720 498
BlpI GC'TnA_GC
Cuts at: 3000 5242 3000
Size: 2242 5583
Bme1580I G_kGCm'C
Cuts at: 1790 2106 3137 5886 7042 1790
Size: 316 1031 2749 1156 2573
Fragments arranged by size:
2749 2573 1156 1031 316
BmgBI CAC'GTC
Cuts at: 1318 2988 3952 1318
Size: 1670 964 5191
Fragments arranged by size:
5191 1670 964
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2629 5556 6514
Size: 416 53 83 399 24 1212 2927 958
Cuts at: 6514 442
Size: 1753
Fragments arranged by size:
2927 1753 1212 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4343 1056
Size: 3287 4538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3345 3345
Size: 7825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2772 4005 6485 1239
Size: 1533 1233 2480 2579
Fragments arranged by size:
2579 2480 1533 1233
Bpu10I CC'TnA_GC
Cuts at: 2067 3975 2067
Size: 1908 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2511 5950 6815 7113 2511
Size: 3439 865 298 3223
Fragments arranged by size:
3439 3223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6488 882
Size: 617 4989 2219
Fragments arranged by size:
4989 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2041 5122 5616 493
Size: 711 837 3081 494 2702
Fragments arranged by size:
3081 2702 837 711 494
BsaBI GATnn'nnATC
Cuts at: 7819 7819
Size: 7825
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3461 5632 6014 6649
Size: 53 83 186 936 1928 2171 382 635
Cuts at: 6649 275
Size: 1451
Fragments arranged by size:
2171 1928 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4482 6258 6656 6930 7077 7488 4482
Size: 1776 398 274 147 411 4819
Fragments arranged by size:
4819 1776 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2116 2146 2850 2880 1178
Size: 30 165 30 713 30 704 30 6123
Fragments arranged by size:
6123 713 704 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2424 2475 2610 2613 2871 1186
Size: 1238 51 135 3 258 6140
Fragments arranged by size:
6140 1238 258 135 51 3
BseYI C'CCAG_C
Cuts at: 2376 2380 3534 3961 7028 7524 2376
Size: 4 1154 427 3067 496 2677
Fragments arranged by size:
3067 2677 1154 496 427 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 2745 1163
Size: 573 580 429 6243
Fragments arranged by size:
6243 580 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5275 6036 7064 1540
Size: 275 186 3274 761 1028 2301
Fragments arranged by size:
3274 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2245 5886 5971 7042 2245
Size: 3641 85 1071 3028
Fragments arranged by size:
3641 3028 1071 85
BsmI GAATG_Cn'
Cuts at: 5336 5429 5336
Size: 93 7732
BsmBI CGTCTCn'nnnn_
Cuts at: 3452 3452
Size: 7825
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2245 2610 3137 5144 5886 5971
Size: 399 316 139 365 527 2007 742 85
Cuts at: 5971 7042 1391
Size: 1071 2174
Fragments arranged by size:
2174 2007 1071 742 527 399 365 316
139 85
BspEI T'CCGG_A
Cuts at: 7488 7488
Size: 7825
BspHI T'CATG_A
Cuts at: 3354 4641 5714 7444 3354
Size: 1287 1073 1730 3735
Fragments arranged by size:
3735 1730 1287 1073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4738 5180 844
Size: 3894 442 3489
Fragments arranged by size:
3894 3489 442
BsrBI CCG'CTC
Cuts at: 1394 1823 5712 1394
Size: 429 3889 3507
Fragments arranged by size:
3889 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2474 4892 6320 6494 65
Size: 1183 1226 2418 1428 174 1396
Fragments arranged by size:
2418 1428 1396 1226 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6469 6656 1700
Size: 110 4659 187 2869
Fragments arranged by size:
4659 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2592 2963 4554 96
Size: 2496 371 1591 3367
Fragments arranged by size:
3367 2496 1591 371
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7825
BssSI C'ACGA_G
Cuts at: 5885 6897 5885
Size: 1012 6813
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3117 794
Size: 2323 5502
BstBI TT'CG_AA
Cuts at: 2007 6629 2007
Size: 4622 3203
BstEII G'GTnAC_C
Cuts at: 1184 2255 1184
Size: 1071 6754
BstXI CCAn_nnnn'nTGG
Cuts at: 2228 4654 2228
Size: 2426 5399
BstYI r'GATC_y
Cuts at: 1972 2113 2522 2800 2892 3281 4602 5100 5155
Size: 141 409 278 92 389 1321 498 55
Cuts at: 5155 5522 5907 5924 7376 7462 7474 7820 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1321 498 409 389 385 367
346 278 141 92 86 55 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4206 4647 513
Size: 552 3141 441 3691
Fragments arranged by size:
3691 3141 552 441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7086
Fragments arranged by size:
7086 683 56
BtsI GCAGTG_nn'
Cuts at: 4206 5341 6135 6155 4206
Size: 1135 794 20 5876
Fragments arranged by size:
5876 1135 794 20
ClaI AT'CG_AT
Cuts at: 1554 4185 1554
Size: 2631 5194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3456 3491 529
Size: 35 2892 35 4863
Fragments arranged by size:
4863 2892 35 35
DraI TTT'AAA
Cuts at: 4405 5129 4405
Size: 724 7101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6832 809
Size: 507 5516 1802
Fragments arranged by size:
5516 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3885 5272 6161
Size: 54 1425 50 275 18 2055 1387 889
Cuts at: 6161 7790 8
Size: 1629 43
Fragments arranged by size:
2055 1629 1425 1387 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5272 1537
Size: 275 3460 4090
Fragments arranged by size:
4090 3460 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2658 3441 3450 3515 3929 5068 5755 1353
Size: 1305 783 9 65 414 1139 687 3423
Fragments arranged by size:
3423 1305 1139 783 687 414 65 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2766 6412 6786 6932 387
Size: 1064 138 1177 3646 374 146 1280
Fragments arranged by size:
3646 1280 1177 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2243 2243
Size: 7825
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2772 3437 3804 4005 4923 5888 6485
Size: 393 1140 665 367 201 918 965 597
Cuts at: 6485 7272 7540 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1140 965 918 787 665 597 393
367 268 201
EcoNI CCTnn'n_nnAGG
Cuts at: 2076 2655 2076
Size: 579 7246
EcoO109I rG'GnC_Cy
Cuts at: 2159 2201 2279 2351 2447 2756 2159
Size: 42 78 72 96 309 7228
Fragments arranged by size:
7228 309 96 78 72 42
EcoRI G'AATT_C
Cuts at: 5134 5512 5134
Size: 378 7447
EcoRV GAT'ATC
Cuts at: 2681 3170 2681
Size: 489 7336
FspI TGC'GCA
Cuts at: 6331 6331
Size: 7825
HaeII r_GCGC'y
Cuts at: 1678 6972 1678
Size: 5294 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2594 2626 2758 2790 2792
Size: 32 135 32 1022 32 132 32 2
Cuts at: 2792 2824 2877 2909 3941 3973 4239 4271 4297
Size: 32 53 32 1032 32 266 32 26
Cuts at: 4297 4329 4357 4389 4587 4619 4623 4655 6466
Size: 32 28 32 198 32 4 32 1811
Cuts at: 6466 6498 6540 6572 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1811 1032 1022 266 198 135 132
53 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
28 26 4 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2030 2293 3604 5169 5415 669
Size: 988 281 92 263 1311 1565 246 3079
Fragments arranged by size:
3079 1565 1311 988 281 263 246 92
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7825
HpaI GTT'AAC
Cuts at: 5415 5415
Size: 7825
KpnI G_GTAC'C
Cuts at: 5150 5150
Size: 7825
MfeI C'AATT_G
Cuts at: 4986 5424 4986
Size: 438 7387
MluI A'CGCG_T
Cuts at: 7701 7701
Size: 7825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2159 3244 3544 3567 3621 4978 5047 6939
Size: 854 1085 300 23 54 1357 69 1892
Cuts at: 6939 7123 1305
Size: 184 2007
Fragments arranged by size:
2007 1892 1357 1085 854 300 184 69
54 23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3887 10
Size: 54 1425 343 2055 3948
Fragments arranged by size:
3948 2055 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4646 4652 4886 5783 6142 6301 518
Size: 648 3480 6 234 897 359 159 2042
Fragments arranged by size:
3480 2042 897 648 359 234 159 6
MspA1I CmG'CkG
Cuts at: 1606 2380 4636 5110 5241 5918 7066 7311 1606
Size: 774 2256 474 131 677 1148 245 2120
Fragments arranged by size:
2256 2120 1148 774 677 474 245 131
NcoI C'CATG_G
Cuts at: 513 1065 4647 513
Size: 552 3582 3691
Fragments arranged by size:
3691 3582 552
NdeI CA'TA_TG
Cuts at: 387 3404 4467 387
Size: 3017 1063 3745
Fragments arranged by size:
3745 3017 1063
NheI G'CTAG_C
Cuts at: 1052 4339 1052
Size: 3287 4538
NotI GC'GGCC_GC
Cuts at: 5272 5272
Size: 7825
NspI r_CATG'y
Cuts at: 1132 1229 2315 3133 3661 4479 4804 5183 6728
Size: 97 1086 818 528 818 325 379 1545
Cuts at: 6728 1132
Size: 2229
Fragments arranged by size:
2229 1545 1086 818 818 528 379 325
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7825
PciI A'CATG_T
Cuts at: 3129 3657 4800 6724 3129
Size: 528 1143 1924 4230
Fragments arranged by size:
4230 1924 1143 528
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2213 2668 3226 4471 1108
Size: 1105 455 558 1245 4462
Fragments arranged by size:
4462 1245 1105 558 455
PfoI T'CCnGG_A
Cuts at: 2212 4437 2212
Size: 2225 5600
PmeI GTTT'AAAC
Cuts at: 5129 5129
Size: 7825
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5894 5926 7407 7439 5894
Size: 32 1481 32 6280
Fragments arranged by size:
6280 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2201 2351 2447 2756 2201
Size: 150 96 309 7270
Fragments arranged by size:
7270 309 150 96
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7825
PsiI TTA'TAA
Cuts at: 4353 5395 4353
Size: 1042 6783
PstI C_TGCA'G
Cuts at: 830 4331 4510 5177 830
Size: 3501 179 667 3478
Fragments arranged by size:
3501 3478 667 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7825
PvuII CAG'CTG
Cuts at: 2380 4636 5110 2380
Size: 2256 474 5095
Fragments arranged by size:
5095 2256 474
SacI G_AGCT'C
Cuts at: 2245 2245
Size: 7825
SalI G'TCGA_C
Cuts at: 1936 5167 1936
Size: 3231 4594
SanDI GG'GwC_CC
Cuts at: 2201 2447 2201
Size: 246 7579
SapI GCTCTTCn'nnn_
Cuts at: 3441 5068 3441
Size: 1627 6198
SbfI CC_TGCA'GG
Cuts at: 5177 5177
Size: 7825
ScaI AGT'ACT
Cuts at: 6073 7785 6073
Size: 1712 6113
SfcI C'TryA_G
Cuts at: 826 1045 2910 4327 4506 5173 6308 6989 7180
Size: 219 1865 1417 179 667 1135 681 191
Cuts at: 7180 826
Size: 1471
Fragments arranged by size:
1865 1471 1417 1135 681 667 219 191
179
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7825
SmaI CCC'GGG
Cuts at: 1872 5152 1872
Size: 3280 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2526 5929 6830 7092 820
Size: 197 926 583 3403 901 262 1553
Fragments arranged by size:
3403 1553 926 901 583 262 197
SnaBI TAC'GTA
Cuts at: 493 5122 5616 493
Size: 4629 494 2702
Fragments arranged by size:
4629 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7825
SphI G_CATG'C
Cuts at: 1132 5183 1132
Size: 4051 3774
SspI AAT'ATT
Cuts at: 5 52 4776 4851 5749 5
Size: 47 4724 75 898 2081
Fragments arranged by size:
4724 2081 898 75 47
StuI AGG'CCT
Cuts at: 1517 2082 3125 1517
Size: 565 1043 6217
Fragments arranged by size:
6217 1043 565
StyI C'CwwG_G
Cuts at: 513 1065 2667 2932 3888 4647 4843 5264 513
Size: 552 1602 265 956 759 196 421 3074
Fragments arranged by size:
3074 1602 956 759 552 421 265 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2390 2560 2660 4640 5852 5869 6022
Size: 1255 810 170 100 1980 1212 17 153
Cuts at: 6022 6207 6652 325
Size: 185 445 1498
Fragments arranged by size:
1980 1498 1255 1212 810 445 185 170
153 100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2592 2963 4554
Size: 274 80 33 51 1443 615 371 1591
Cuts at: 4554 4582 4718 6071 7783 96
Size: 28 136 1353 1712 138
Fragments arranged by size:
1712 1591 1443 1353 615 371 274 138
136 80 51 33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2116 2148 4477 4509 7785 7817 2116
Size: 32 2329 32 3276 32 2124
Fragments arranged by size:
3276 2329 2124 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7825
XbaI T'CTAG_A
Cuts at: 5161 5161
Size: 7825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2450 3895 2450
Size: 1445 6380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7825
XmaI C'CCGG_G
Cuts at: 1870 5150 1870
Size: 3280 4545
XmnI GAAnn'nnTTC
Cuts at: 3435 3752 5954 3435
Size: 317 2202 5306
Fragments arranged by size:
5306 2202 317
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3462 5633 276
Size: 53 83 186 936 1928 2171 2468
Fragments arranged by size:
2468 2171 1928 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI
SwaI