Restriction map for
pFN21ASDA0752
(Circular) (Six-base) MAPSORT of: pFN21ASDA0752.seq Check: 996 from: 1 to: 5659
pFN21ASDA0752 5659 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2617 2719 2827 2617
Size: 102 108 5449
Fragments arranged by size:
5449 108 102
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2313 2459 3722 5106 5374 1632
Size: 681 146 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 681 268 146
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 2225 2399 2482 2597 2699 3402 4974 2225
Size: 174 83 115 102 703 1572 2910
Fragments arranged by size:
2910 1572 703 174 115 102 83
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 2984 1870
Size: 73 252 789 4545
Fragments arranged by size:
4545 789 252 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2339 2980 4435 618
Size: 325 217 627 552 641 1455 1842
Fragments arranged by size:
1842 1455 641 627 552 325 217
BanII G_rGCy'C
Cuts at: 1391 2088 2978 1391
Size: 697 890 4072
Fragments arranged by size:
4072 890 697
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 2370 2531 2568 2670 2769 928
Size: 971 236 235 161 37 102 99 3818
Fragments arranged by size:
3818 971 236 235 161 102 99 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2460 1548
Size: 912 4747
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2645 2747 4270
Size: 107 122 71 631 605 973 102 1523
Cuts at: 4270 136
Size: 1525
Fragments arranged by size:
1525 1523 973 631 605 122 107 102
71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4876 1790
Size: 1930 1156 2573
Fragments arranged by size:
2573 1930 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2221 2221
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 2588 2690 4319 1239
Size: 1151 198 102 1629 2579
Fragments arranged by size:
2579 1629 1151 198 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2956 3450 493
Size: 711 1752 494 2702
Fragments arranged by size:
2702 1752 711 494
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2070 2100 2153 2183 1178
Size: 30 165 30 667 30 53 30 4654
Fragments arranged by size:
4654 667 165 53 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2303 2387 2516 2519 2531 2585 2687
Size: 1096 21 84 129 3 12 54 102
Cuts at: 2687 1186
Size: 4158
Fragments arranged by size:
4158 1096 129 102 84 54 21 12
3
BseYI C'CCAG_C
Cuts at: 2635 2737 2897 4862 5358 2635
Size: 102 160 1965 496 2936
Fragments arranged by size:
2936 1965 496 160 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2799 1163
Size: 573 1063 4023
Fragments arranged by size:
4023 1063 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 3720 3805 4876 2088
Size: 1632 85 1071 2871
Fragments arranged by size:
2871 1632 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
BsmBI CGTCTCn'nnnn_
Cuts at: 2204 2204
Size: 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2978 3720 3805 4876 1391
Size: 399 298 890 742 85 1071 2174
Fragments arranged by size:
2174 1071 890 742 399 298 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2617 2719 2827 3014 844
Size: 1773 102 108 187 3489
Fragments arranged by size:
3489 1773 187 108 102
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4154 4328 65
Size: 1183 2906 174 1396
Fragments arranged by size:
2906 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2910 4303 4490 1700
Size: 110 1100 1393 187 2869
Fragments arranged by size:
2869 1393 1100 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 2007 4463 2007
Size: 2456 3203
BstEII G'GTnAC_C
Cuts at: 1184 2354 2552 1184
Size: 1170 198 4291
Fragments arranged by size:
4291 1170 198
BstYI r'GATC_y
Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654
Size: 1017 367 385 17 1452 86 12 346
Cuts at: 5654 1972
Size: 1977
Fragments arranged by size:
1977 1452 1017 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4150
Fragments arranged by size:
4150 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2138 487
Size: 683 56 912 4008
Fragments arranged by size:
4008 912 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 3969 3989 3175
Size: 794 20 4845
Fragments arranged by size:
4845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DraIII CAC_nnn'GTG
Cuts at: 2153 2549 2153
Size: 396 5263
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2448 3106 3995
Size: 54 1425 50 275 18 618 658 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 658 618 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2847 3589 1353
Size: 1494 742 3423
Fragments arranged by size:
3423 1494 742
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2086 2086
Size: 5659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2313 2390 2459 2588 2690 3722 4319
Size: 393 681 77 69 129 102 1032 597
Cuts at: 4319 5106 5374 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1032 787 681 597 393 268 129
102 77 69
EcoO109I rG'GnC_Cy
Cuts at: 2434 2509 2434
Size: 75 5584
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2548 2152
Size: 396 5263
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 4300 4332 4374
Size: 32 135 32 581 32 2115 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 2115 581 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 2756 3258 2756
Size: 502 5157
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 3617 3976 4135 518
Size: 648 888 1563 359 159 2042
Fragments arranged by size:
2042 1563 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3075 3752 4900 5145 1606
Size: 1469 677 1148 245 2120
Fragments arranged by size:
2120 1469 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4150
Fragments arranged by size:
4150 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2579 2681 3017 4562 1132
Size: 97 1350 102 336 1545 2229
Fragments arranged by size:
2229 1545 1350 336 102 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2549 1108
Size: 1441 4218
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2434 2509 2434
Size: 75 5584
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2824 3011 830
Size: 1994 187 3478
Fragments arranged by size:
3478 1994 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
SacI G_AGCT'C
Cuts at: 2088 2088
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SanDI GG'GwC_CC
Cuts at: 2434 2434
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2820 3007 4142 4823 5014 826
Size: 219 1775 187 1135 681 191 1471
Fragments arranged by size:
1775 1471 1135 681 219 191 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3763 4664 4926 820
Size: 197 926 1820 901 262 1553
Fragments arranged by size:
1820 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2956 3450 493
Size: 2463 494 2702
Fragments arranged by size:
2702 2463 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2022 2136 2172 2532 3098 513
Size: 552 957 114 36 360 566 3074
Fragments arranged by size:
3074 957 566 552 360 114 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 3686 3703 3856 4041 4486 325
Size: 1255 777 1329 17 153 185 445 1498
Fragments arranged by size:
1498 1329 1255 777 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2470 3905 5617
Size: 274 80 33 51 1443 493 1435 1712
Cuts at: 5617 96
Size: 138
Fragments arranged by size:
1712 1443 1435 493 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2263 2995 2263
Size: 732 4927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI