Restriction map for
pFN21ASDA0736
(Circular) (Six-base) MAPSORT of: pFN21ASDA0736.seq Check: 6162 from: 1 to: 6955
pFN21ASDA0736 6955 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4228 4228
Size: 6955
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4765 278
Size: 53 83 186 936 3229 2468
Fragments arranged by size:
3229 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2655 3813 4298 1937
Size: 718 1158 485 4594
Fragments arranged by size:
4594 1158 718 485
Acc65I G'GTAC_C
Cuts at: 3270 4276 3270
Size: 1006 5949
AclI AA'CG_TT
Cuts at: 1563 5082 5455 1563
Size: 3519 373 3063
Fragments arranged by size:
3519 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3164 4235 5018 6402 6670 1632
Size: 1532 1071 783 1384 268 1917
Fragments arranged by size:
1917 1532 1384 1071 783 268
AfeI AGC'GCT
Cuts at: 2599 3278 2599
Size: 679 6276
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6758
AflIII A'CryG_T
Cuts at: 1203 2610 3651 4032 5854 6831 1203
Size: 1407 1041 381 1822 977 1327
Fragments arranged by size:
1822 1407 1327 1041 977 381
AgeI A'CCGG_T
Cuts at: 5786 5786
Size: 6955
AhdI GACnn_n'nnGTC
Cuts at: 5684 5684
Size: 6955
AleI CACnn'nnGTG
Cuts at: 1166 3476 1166
Size: 2310 4645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6955
AlwNI CAG_nnn'CTG
Cuts at: 2339 2725 4243 4698 6270 2339
Size: 386 1518 455 1572 3024
Fragments arranged by size:
3024 1572 1518 455 386
ApaLI G'TGCA_C
Cuts at: 5012 6168 5012
Size: 1156 5799
ApoI r'AATT_y
Cuts at: 1493 3322 4264 4490 4642 6751 1493
Size: 1829 942 226 152 2109 1697
Fragments arranged by size:
2109 1829 1697 942 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5509 160
Size: 872 4477 1606
Fragments arranged by size:
4477 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6955
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 3485 4230 4280 1870
Size: 73 385 1157 745 50 4545
Fragments arranged by size:
4545 1157 745 385 73 50
AvrII C'CTAG_G
Cuts at: 3301 3301
Size: 6955
BamHI G'GATC_C
Cuts at: 4285 4652 4285
Size: 367 6588
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2371 3270 3800 4276 5731
Size: 325 217 627 584 899 530 476 1455
Cuts at: 5731 618
Size: 1842
Fragments arranged by size:
1842 1455 899 627 584 530 476 325
217
BanII G_rGCy'C
Cuts at: 1391 3073 3095 4274 1391
Size: 1682 22 1179 4072
Fragments arranged by size:
4072 1682 1179 22
BbeI G_GCGC'C
Cuts at: 2375 2375
Size: 6955
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2746 2842 3019 928
Size: 971 847 96 177 4864
Fragments arranged by size:
4864 971 847 177 96
BbvCI CC'TCA_GC
Cuts at: 4180 4180
Size: 6955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5146 5180 1903
Size: 34 3209 34 3678
Fragments arranged by size:
3678 3209 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4847 6057 4847
Size: 1210 5745
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6955
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3068 3996 5566
Size: 107 122 71 631 605 1396 928 1570
Cuts at: 5566 136
Size: 1525
Fragments arranged by size:
1570 1525 1396 928 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2298 6950 2298
Size: 4652 2303
BlpI GC'TnA_GC
Cuts at: 4372 4372
Size: 6955
Bme1580I G_kGCm'C
Cuts at: 1790 5016 6172 1790
Size: 3226 1156 2573
Fragments arranged by size:
3226 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3781 4033 1318
Size: 2463 252 4240
Fragments arranged by size:
4240 2463 252
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2199 4686 5644
Size: 416 53 83 399 24 782 2487 958
Cuts at: 5644 442
Size: 1753
Fragments arranged by size:
2487 1753 958 782 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6955
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2699
Size: 6955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 3040 3431 3898 5615 1239
Size: 1164 637 391 467 1717 2579
Fragments arranged by size:
2579 1717 1164 637 467 391
Bpu10I CC'TnA_GC
Cuts at: 2079 3186 4180 2079
Size: 1107 994 4854
Fragments arranged by size:
4854 1107 994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2419 3316 3569 5080 5945 6243 2419
Size: 897 253 1511 865 298 3131
Fragments arranged by size:
3131 1511 897 865 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2034 4220 5618 882
Size: 617 535 2186 1398 2219
Fragments arranged by size:
2219 2186 1398 617 535
BsaAI yAC'GTr
Cuts at: 493 1204 4252 4746 493
Size: 711 3048 494 2702
Fragments arranged by size:
3048 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 3245 4127 6949 3245
Size: 882 2822 3251
Fragments arranged by size:
3251 2822 882
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2372 4762 5144 5779
Size: 53 83 186 936 839 2390 382 635
Cuts at: 5779 275
Size: 1451
Fragments arranged by size:
2390 1451 936 839 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5388 5786 6060 6207 6618 5388
Size: 398 274 147 411 5725
Fragments arranged by size:
5725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2707 2737 1178
Size: 30 165 30 1304 30 5396
Fragments arranged by size:
5396 1304 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2189 2238 2258 1186
Size: 1000 3 49 20 5883
Fragments arranged by size:
5883 1000 49 20 3
BseYI C'CCAG_C
Cuts at: 2594 2707 3279 4117 6158 6654 2594
Size: 113 572 838 2041 496 2895
Fragments arranged by size:
2895 2041 838 572 496 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3652 3737 4146 1163
Size: 573 1916 85 409 3972
Fragments arranged by size:
3972 1916 573 409 85
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2509 4405 5166 6194 1540
Size: 275 186 508 1896 761 1028 2301
Fragments arranged by size:
2301 1896 1028 761 508 275 186
BsiHKAI G_wGCw'C
Cuts at: 5016 5101 6172 5016
Size: 85 1071 5799
Fragments arranged by size:
5799 1071 85
BsmI GAATG_Cn'
Cuts at: 2322 3906 4027 4096 4466 4559 2322
Size: 1584 121 69 370 93 4718
Fragments arranged by size:
4718 1584 370 121 93 69
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3073 3095 4274 5016 5101 6172 1391
Size: 399 1283 22 1179 742 85 1071 2174
Fragments arranged by size:
2174 1283 1179 1071 742 399 85 22
BspEI T'CCGG_A
Cuts at: 6618 6618
Size: 6955
BspHI T'CATG_A
Cuts at: 4844 6574 4844
Size: 1730 5225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3139 3301 4228 4310 844
Size: 2295 162 927 82 3489
Fragments arranged by size:
3489 2295 927 162 82
BsrBI CCG'CTC
Cuts at: 1394 1823 2356 4842 1394
Size: 429 533 2486 3507
Fragments arranged by size:
3507 2486 533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2073 4008 5450 5624 65
Size: 1183 825 1935 1442 174 1396
Fragments arranged by size:
1935 1442 1396 1183 825 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2703 5599 5786 1700
Size: 110 893 2896 187 2869
Fragments arranged by size:
2896 2869 893 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6955
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6955
BssSI C'ACGA_G
Cuts at: 5015 6027 5015
Size: 1012 5943
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2725 4243 794
Size: 1931 1518 3506
Fragments arranged by size:
3506 1931 1518
BstBI TT'CG_AA
Cuts at: 2007 5759 2007
Size: 3752 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6955
BstXI CCAn_nnnn'nTGG
Cuts at: 2110 2110
Size: 6955
BstYI r'GATC_y
Cuts at: 1972 2298 3246 3534 4285 4652 5037 5054 6506
Size: 326 948 288 751 367 385 17 1452
Cuts at: 6506 6592 6604 6950 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 948 751 385 367 346 326
288 86 17 12
BstZ17I GTA'TAC
Cuts at: 3814 3814
Size: 6955
BtgI C'CryG_G
Cuts at: 513 1065 2022 2059 2503 513
Size: 552 957 37 444 4965
Fragments arranged by size:
4965 957 552 444 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6216
Fragments arranged by size:
6216 683 56
BtsI GCAGTG_nn'
Cuts at: 2726 3459 3849 4471 5265 5285 2726
Size: 733 390 622 794 20 4396
Fragments arranged by size:
4396 794 733 622 390 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6955
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4060 4095 529
Size: 35 3496 35 3389
Fragments arranged by size:
3496 3389 35 35
DraI TTT'AAA
Cuts at: 4259 4259
Size: 6955
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5962 809
Size: 507 4646 1802
Fragments arranged by size:
4646 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2500 2506
Size: 54 1425 50 275 18 270 400 6
Cuts at: 2506 3969 4402 5291 6920 8
Size: 1463 433 889 1629 43
Fragments arranged by size:
1629 1463 1425 889 433 400 275 270
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2506 4402 1537
Size: 275 694 1896 4090
Fragments arranged by size:
4090 1896 694 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2022 2804 3612 4885 1353
Size: 669 782 808 1273 3423
Fragments arranged by size:
3423 1273 808 782 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3428 5542 5916 6062 387
Size: 1064 138 1839 2114 374 146 1280
Fragments arranged by size:
2114 1839 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 3040 3164 3431 3898 4235 5018
Size: 393 771 637 124 267 467 337 783
Cuts at: 5018 5615 6402 6670 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 783 771 637 597 467 393
337 268 267 124
EcoO109I rG'GnC_Cy
Cuts at: 2119 2232 2414 3264 2119
Size: 113 182 850 5810
Fragments arranged by size:
5810 850 182 113
EcoRI G'AATT_C
Cuts at: 4264 4642 4264
Size: 378 6577
FspI TGC'GCA
Cuts at: 5461 5461
Size: 6955
HaeII r_GCGC'y
Cuts at: 1678 2375 2549 2601 3280 6102 1678
Size: 697 174 52 679 2822 2531
Fragments arranged by size:
2822 2531 697 679 174 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2707 2739 2823 2855 3208
Size: 32 135 32 1135 32 84 32 353
Cuts at: 3208 3236 3240 3268 3427 3449 3459 3481 3626
Size: 28 4 28 159 22 10 22 145
Cuts at: 3626 3658 5596 5628 5670 5702 1373
Size: 32 1938 32 42 32 2626
Fragments arranged by size:
2626 1938 1135 353 159 145 135 84
42 32 32 32 32 32 32 32
28 28 22 22 10 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2746 4038 4299 4545 669
Size: 988 281 808 1292 261 246 3079
Fragments arranged by size:
3079 1292 988 808 281 261 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6955
HpaI GTT'AAC
Cuts at: 4545 4545
Size: 6955
KasI G'GCGC_C
Cuts at: 2371 2371
Size: 6955
KpnI G_GTAC'C
Cuts at: 3274 4280 3274
Size: 1006 5949
MfeI C'AATT_G
Cuts at: 4554 4554
Size: 6955
MluI A'CGCG_T
Cuts at: 6831 6831
Size: 6955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2646 2652 2693 3234 3504 6069 6253 1305
Size: 1341 6 41 541 270 2565 184 2007
Fragments arranged by size:
2565 2007 1341 541 270 184 41 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2502 10
Size: 54 1425 343 670 4463
Fragments arranged by size:
4463 1425 670 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 3365 3476 4913 5272 5431 518
Size: 648 1503 696 111 1437 359 159 2042
Fragments arranged by size:
2042 1503 1437 696 648 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 2945 3018 4112 4184 4371 5048 6196 6441
Size: 1339 73 1094 72 187 677 1148 245
Cuts at: 6441 1606
Size: 2120
Fragments arranged by size:
2120 1339 1148 1094 677 245 187 73
72
NarI GG'CG_CC
Cuts at: 2372 2372
Size: 6955
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5446
Fragments arranged by size:
5446 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6955
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6955
NotI GC'GGCC_GC
Cuts at: 4402 4402
Size: 6955
NspI r_CATG'y
Cuts at: 1132 1229 2099 2614 2641 2917 3169 3506 4313
Size: 97 870 515 27 276 252 337 807
Cuts at: 4313 5858 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 870 807 515 337 276 252
97 27
PasI CC'CwG_GG
Cuts at: 1348 2420 1348
Size: 1072 5883
PciI A'CATG_T
Cuts at: 2610 5854 2610
Size: 3244 3711
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3454 1108
Size: 2346 4609
PfoI T'CCnGG_A
Cuts at: 2133 4016 2133
Size: 1883 5072
PmeI GTTT'AAAC
Cuts at: 4259 4259
Size: 6955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 5024 5056 6537 6569 3179
Size: 32 1813 32 1481 32 3565
Fragments arranged by size:
3565 1813 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2232 2414 3264 2232
Size: 182 850 5923
Fragments arranged by size:
5923 850 182
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6955
PsiI TTA'TAA
Cuts at: 4525 4525
Size: 6955
PstI C_TGCA'G
Cuts at: 830 4248 4307 830
Size: 3418 59 3478
Fragments arranged by size:
3478 3418 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6955
PvuII CAG'CTG
Cuts at: 2945 3018 4112 4184 2945
Size: 73 1094 72 5716
Fragments arranged by size:
5716 1094 73 72
SalI G'TCGA_C
Cuts at: 1936 4297 1936
Size: 2361 4594
SanDI GG'GwC_CC
Cuts at: 2232 2414 2232
Size: 182 6773
SbfI CC_TGCA'GG
Cuts at: 4307 4307
Size: 6955
ScaI AGT'ACT
Cuts at: 3554 5203 6915 3554
Size: 1649 1712 3594
Fragments arranged by size:
3594 1712 1649
SexAI A'CCwGG_T
Cuts at: 3266 3266
Size: 6955
SfcI C'TryA_G
Cuts at: 826 1045 4244 4303 5438 6119 6310 826
Size: 219 3199 59 1135 681 191 1471
Fragments arranged by size:
3199 1471 1135 681 219 191 59
SfoI GGC'GCC
Cuts at: 2373 2373
Size: 6955
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6955
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6955
SmaI CCC'GGG
Cuts at: 1872 2330 3487 4232 4282 1872
Size: 458 1157 745 50 4545
Fragments arranged by size:
4545 1157 745 458 50
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2398 3295 3584 5059 5960 6222
Size: 197 926 455 897 289 1475 901 262
Cuts at: 6222 820
Size: 1553
Fragments arranged by size:
1553 1475 926 901 897 455 289 262
197
SnaBI TAC'GTA
Cuts at: 493 4252 4746 493
Size: 3759 494 2702
Fragments arranged by size:
3759 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6955
SphI G_CATG'C
Cuts at: 1132 2099 2641 4313 1132
Size: 967 542 1672 3774
Fragments arranged by size:
3774 1672 967 542
SspI AAT'ATT
Cuts at: 5 52 2144 4879 5
Size: 47 2092 2735 2081
Fragments arranged by size:
2735 2092 2081 47
StuI AGG'CCT
Cuts at: 1517 2646 3131 1517
Size: 1129 485 5341
Fragments arranged by size:
5341 1129 485
StyI C'CwwG_G
Cuts at: 513 1065 2022 3301 4155 4195 4394 513
Size: 552 957 1279 854 40 199 3074
Fragments arranged by size:
3074 1279 957 854 552 199 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2967 3365 4180 4982 4999 5152 5337
Size: 1255 1387 398 815 802 17 153 185
Cuts at: 5337 5782 325
Size: 445 1498
Fragments arranged by size:
1498 1387 1255 815 802 445 398 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3552 3711 3743
Size: 274 80 33 51 1443 1575 159 32
Cuts at: 3743 5201 6913 96
Size: 1458 1712 138
Fragments arranged by size:
1712 1575 1458 1443 274 159 138 80
51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2914 2946 6915 6947 2914
Size: 32 3969 32 2922
Fragments arranged by size:
3969 2922 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 1316
Size: 813 6142
XbaI T'CTAG_A
Cuts at: 4291 4291
Size: 6955
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6955
XmaI C'CCGG_G
Cuts at: 1870 2328 3485 4230 4280 1870
Size: 458 1157 745 50 4545
Fragments arranged by size:
4545 1157 745 458 50
XmnI GAAnn'nnTTC
Cuts at: 5084 5084
Size: 6955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4763 276
Size: 53 83 186 936 3229 2468
Fragments arranged by size:
3229 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BfrBI BsiWI BsmBI Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SfiI SrfI SwaI XcmI