Restriction map for
pFN21ASDA0717
(Circular) (Six-base) MAPSORT of: pFN21ASDA0717.seq Check: 5014 from: 1 to: 6910
pFN21ASDA0717 6910 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2670 2670
Size: 6910
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 2826 3858 4720
Size: 53 83 186 936 1083 207 1032 862
Cuts at: 4720 278
Size: 2468
Fragments arranged by size:
2468 1083 1032 936 862 207 186 83
53
AccI GT'mk_AC
Cuts at: 1937 4203 4253 1937
Size: 2266 50 4594
Fragments arranged by size:
4594 2266 50
Acc65I G'GTAC_C
Cuts at: 2388 3153 4032 4231 2388
Size: 765 879 199 5067
Fragments arranged by size:
5067 879 765 199
AclI AA'CG_TT
Cuts at: 1563 5037 5410 1563
Size: 3474 373 3063
Fragments arranged by size:
3474 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4058 4973 6357 6625 1632
Size: 2426 915 1384 268 1917
Fragments arranged by size:
2426 1917 1384 915 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6713
AflIII A'CryG_T
Cuts at: 1203 3060 3945 5809 6786 1203
Size: 1857 885 1864 977 1327
Fragments arranged by size:
1864 1857 1327 977 885
AgeI A'CCGG_T
Cuts at: 5741 5741
Size: 6910
AhdI GACnn_n'nnGTC
Cuts at: 5639 5639
Size: 6910
AleI CACnn'nnGTG
Cuts at: 1166 2168 4027 1166
Size: 1002 1859 4049
Fragments arranged by size:
4049 1859 1002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6910
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 6878
AlwNI CAG_nnn'CTG
Cuts at: 2158 4653 6225 2158
Size: 2495 1572 2843
Fragments arranged by size:
2843 2495 1572
ApaI G_GGCC'C
Cuts at: 2945 3297 2945
Size: 352 6558
ApaLI G'TGCA_C
Cuts at: 4967 6123 4967
Size: 1156 5754
ApoI r'AATT_y
Cuts at: 1493 4219 4445 4597 6706 1493
Size: 2726 226 152 2109 1697
Fragments arranged by size:
2726 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5464 160
Size: 872 4432 1606
Fragments arranged by size:
4432 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 2415 3386 3636 3963 4235 1870
Size: 73 286 186 971 250 327 272 4545
Fragments arranged by size:
4545 971 327 286 272 250 186 73
BamHI G'GATC_C
Cuts at: 4240 4607 4240
Size: 367 6543
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 2713 2755 2960 3153
Size: 325 217 627 601 325 42 205 193
Cuts at: 3153 3512 3548 4032 4231 5686 618
Size: 359 36 484 199 1455 1842
Fragments arranged by size:
1842 1455 627 601 484 359 325 325
217 205 199 193 42 36
BanII G_rGCy'C
Cuts at: 1391 2945 3092 3297 3632 4229 1391
Size: 1554 147 205 335 597 4072
Fragments arranged by size:
4072 1554 597 335 205 147
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3174 4146 928
Size: 971 1275 972 3692
Fragments arranged by size:
3692 1275 972 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5101 5135 1903
Size: 34 3164 34 3678
Fragments arranged by size:
3678 3164 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4802 6012 4802
Size: 1210 5700
BclI T'GATC_A
Cuts at: 1548 2992 1548
Size: 1444 5466
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4117 5521 136
Size: 107 122 71 631 605 2445 1404 1525
Fragments arranged by size:
2445 1525 1404 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2318 2868 6905 2318
Size: 550 4037 2323
Fragments arranged by size:
4037 2323 550
BlpI GC'TnA_GC
Cuts at: 3378 4327 3378
Size: 949 5961
Bme1580I G_kGCm'C
Cuts at: 1790 2122 2175 2760 2789 2945 2963 3297 4066
Size: 332 53 585 29 156 18 334 769
Cuts at: 4066 4971 6127 1790
Size: 905 1156 2573
Fragments arranged by size:
2573 1156 905 769 585 334 332 156
53 29 18
BmgBI CAC'GTC
Cuts at: 1318 2236 3061 3472 1318
Size: 918 825 411 4756
Fragments arranged by size:
4756 918 825 411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2140 2593 4641
Size: 416 53 83 399 24 723 453 2048
Cuts at: 4641 5599 442
Size: 958 1753
Fragments arranged by size:
2048 1753 958 723 453 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6910
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2660
Size: 6910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 2755 2822 3208 3700 5570 1239
Size: 1250 266 67 386 492 1870 2579
Fragments arranged by size:
2579 1870 1250 492 386 266 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3296 4085 5035 5900 6198 3296
Size: 789 950 865 298 4008
Fragments arranged by size:
4008 950 865 789 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2054 2277 2576 5573 882
Size: 617 555 223 299 2997 2219
Fragments arranged by size:
2997 2219 617 555 299 223
BsaAI yAC'GTr
Cuts at: 493 1204 4207 4701 493
Size: 711 3003 494 2702
Fragments arranged by size:
3003 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6904 6904
Size: 6910
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 2823 3855 4717
Size: 53 83 186 936 1083 207 1032 862
Cuts at: 4717 5099 5734 275
Size: 382 635 1451
Fragments arranged by size:
1451 1083 1032 936 862 635 382 207
186 83 53
BsaWI w'CCGG_w
Cuts at: 3092 5343 5741 6015 6162 6573 3092
Size: 2251 398 274 147 411 3429
Fragments arranged by size:
3429 2251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2753 2783 3632 3662 1178
Size: 30 165 30 1350 30 849 30 4426
Fragments arranged by size:
4426 1350 849 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2795 2875 2878 4159 4162 4180 1186
Size: 1609 80 3 1281 3 18 3916
Fragments arranged by size:
3916 1609 1281 80 18 3 3
BseYI C'CCAG_C
Cuts at: 2563 2935 3083 6113 6609 2563
Size: 372 148 3030 496 2864
Fragments arranged by size:
3030 2864 496 372 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2625 2714 3913 1163
Size: 573 889 89 1199 4160
Fragments arranged by size:
4160 1199 889 573 89
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4360 5121 6149 1540
Size: 275 186 2359 761 1028 2301
Fragments arranged by size:
2359 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2422 3632 4971 5056 6127 2422
Size: 1210 1339 85 1071 3205
Fragments arranged by size:
3205 1339 1210 1071 85
BsmI GAATG_Cn'
Cuts at: 4421 4514 4421
Size: 93 6817
BsmBI CGTCTCn'nnnn_
Cuts at: 2226 2260 2226
Size: 34 6876
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2175 2422 2760 2789 2945 2963
Size: 399 332 53 247 338 29 156 18
Cuts at: 2963 3092 3297 3632 4066 4229 4971 5056 6127
Size: 129 205 335 434 163 742 85 1071
Cuts at: 6127 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 434 399 338 335 332
247 205 163 156 129 85 53 29
18
BspEI T'CCGG_A
Cuts at: 6573 6573
Size: 6910
BspHI T'CATG_A
Cuts at: 3441 4799 6529 3441
Size: 1358 1730 3822
Fragments arranged by size:
3822 1730 1358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2460 2670 4265 844
Size: 1616 210 1595 3489
Fragments arranged by size:
3489 1616 1595 210
BsrBI CCG'CTC
Cuts at: 1394 1823 2812 2845 4797 1394
Size: 429 989 33 1952 3507
Fragments arranged by size:
3507 1952 989 429 33
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 2346 2695 3363 3372 5405 5579
Size: 1183 889 209 349 668 9 2033 174
Cuts at: 5579 65
Size: 1396
Fragments arranged by size:
2033 1396 1183 889 668 349 209 174
9
BsrFI r'CCGG_y
Cuts at: 1700 1810 3701 5554 5741 1700
Size: 110 1891 1853 187 2869
Fragments arranged by size:
2869 1891 1853 187 110
BsrGI T'GTAC_A
Cuts at: 96 3321 96
Size: 3225 3685
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6910
BssSI C'ACGA_G
Cuts at: 4970 5982 4970
Size: 1012 5898
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3669 794
Size: 2875 4035
BstBI TT'CG_AA
Cuts at: 2007 5714 2007
Size: 3707 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6910
BstXI CCAn_nnnn'nTGG
Cuts at: 2563 2593 3023 4029 2563
Size: 30 430 1006 5444
Fragments arranged by size:
5444 1006 430 30
BstYI r'GATC_y
Cuts at: 1972 2318 2868 3129 3229 4240 4607 4992 5009
Size: 346 550 261 100 1011 367 385 17
Cuts at: 5009 6461 6547 6559 6905 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1011 550 385 367 346 346
261 100 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2094 3022 513
Size: 552 957 72 928 4401
Fragments arranged by size:
4401 957 928 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6171
Fragments arranged by size:
6171 683 56
BtsI GCAGTG_nn'
Cuts at: 4426 5220 5240 4426
Size: 794 20 6096
Fragments arranged by size:
6096 794 20
ClaI AT'CG_AT
Cuts at: 1554 3846 1554
Size: 2292 4618
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6875
DraI TTT'AAA
Cuts at: 4214 4214
Size: 6910
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5917 809
Size: 507 4601 1802
Fragments arranged by size:
4601 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2553 3414
Size: 54 1425 50 275 18 330 393 861
Cuts at: 3414 3903 4019 4357 5246 6875 8
Size: 489 116 338 889 1629 43
Fragments arranged by size:
1629 1425 889 861 489 393 338 330
275 116 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4357 1537
Size: 275 2545 4090
Fragments arranged by size:
4090 2545 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3210 4133 4840 1353
Size: 1857 923 707 3423
Fragments arranged by size:
3423 1857 923 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3464 5497 5871 6017 387
Size: 1064 138 1875 2033 374 146 1280
Fragments arranged by size:
2033 1875 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3630 3630
Size: 6910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 2755 2822 3208 3700 4058 4973
Size: 393 857 266 67 386 492 358 915
Cuts at: 4973 5570 6357 6625 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 915 857 787 597 492 393 386
358 268 266 67
EcoNI CCTnn'n_nnAGG
Cuts at: 2500 2801 2924 4040 2500
Size: 301 123 1116 5370
Fragments arranged by size:
5370 1116 301 123
EcoO109I rG'GnC_Cy
Cuts at: 2806 2941 2942 3293 3294 2806
Size: 135 1 351 1 6422
Fragments arranged by size:
6422 351 135 1 1
EcoRI G'AATT_C
Cuts at: 4219 4597 4219
Size: 378 6532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 3517 2635
Size: 882 6028
FspI TGC'GCA
Cuts at: 2852 3404 5416 2852
Size: 552 2012 4346
Fragments arranged by size:
4346 2012 552
FspAI rTGC'GCAy
Cuts at: 2852 2852
Size: 6910
HaeII r_GCGC'y
Cuts at: 1678 3561 6057 1678
Size: 1883 2496 2531
Fragments arranged by size:
2531 2496 1883
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2409 2441 2753 2785 5551
Size: 32 135 32 837 32 312 32 2766
Cuts at: 5551 5583 5625 5657 1373
Size: 32 42 32 2626
Fragments arranged by size:
2766 2626 837 312 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2479 4254 4500 669
Size: 988 281 541 1775 246 3079
Fragments arranged by size:
3079 1775 988 541 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6910
HpaI GTT'AAC
Cuts at: 4500 4500
Size: 6910
KpnI G_GTAC'C
Cuts at: 2392 3157 4036 4235 2392
Size: 765 879 199 5067
Fragments arranged by size:
5067 879 765 199
MfeI C'AATT_G
Cuts at: 4509 4509
Size: 6910
MluI A'CGCG_T
Cuts at: 6786 6786
Size: 6910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2427 6024 6208 1305
Size: 1122 3597 184 2007
Fragments arranged by size:
3597 2007 1122 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 2555 3416 4021 10
Size: 54 1425 343 330 393 861 605 2899
Fragments arranged by size:
2899 1425 861 605 393 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2432 2753 2831 3021 3410 3704
Size: 648 1002 264 321 78 190 389 294
Cuts at: 3704 4027 4868 5227 5386 518
Size: 323 841 359 159 2042
Fragments arranged by size:
2042 1002 841 648 389 359 323 321
294 264 190 159 78
MspA1I CmG'CkG
Cuts at: 1606 2155 2698 3585 3901 4060 4326 5003 6151
Size: 549 543 887 316 159 266 677 1148
Cuts at: 6151 6396 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 887 677 549 543 316 266
245 159
NcoI C'CATG_G
Cuts at: 513 1065 2022 3022 513
Size: 552 957 1000 4401
Fragments arranged by size:
4401 1000 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6910
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6910
NotI GC'GGCC_GC
Cuts at: 4357 4357
Size: 6910
NspI r_CATG'y
Cuts at: 1132 1229 2171 3676 3709 4268 5813 1132
Size: 97 942 1505 33 559 1545 2229
Fragments arranged by size:
2229 1545 1505 942 559 97 33
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6910
PciI A'CATG_T
Cuts at: 5809 5809
Size: 6910
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2158 2181 2800 3835 1108
Size: 1050 23 619 1035 4183
Fragments arranged by size:
4183 1050 1035 619 23
PfoI T'CCnGG_A
Cuts at: 2799 3185 3210 3537 3867 2799
Size: 386 25 327 330 5842
Fragments arranged by size:
5842 386 330 327 25
PmeI GTTT'AAAC
Cuts at: 4214 4214
Size: 6910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4979 5011 6492 6524 4979
Size: 32 1481 32 5365
Fragments arranged by size:
5365 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2806 2806
Size: 6910
PshAI GACnn'nnGTC
Cuts at: 1613 3912 1613
Size: 2299 4611
PsiI TTA'TAA
Cuts at: 4480 4480
Size: 6910
PspOMI G'GGCC_C
Cuts at: 2941 3293 2941
Size: 352 6558
PspXI vC'TCGA_Gb
Cuts at: 3386 3386
Size: 6910
PstI C_TGCA'G
Cuts at: 830 2667 2712 2841 4262 830
Size: 1837 45 129 1421 3478
Fragments arranged by size:
3478 1837 1421 129 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6910
PvuII CAG'CTG
Cuts at: 2155 3585 3901 2155
Size: 1430 316 5164
Fragments arranged by size:
5164 1430 316
SacI G_AGCT'C
Cuts at: 3632 3632
Size: 6910
SalI G'TCGA_C
Cuts at: 1936 4252 1936
Size: 2316 4594
SapI GCTCTTCn'nnn_
Cuts at: 4133 4133
Size: 6910
SbfI CC_TGCA'GG
Cuts at: 4262 4262
Size: 6910
ScaI AGT'ACT
Cuts at: 5158 6870 5158
Size: 1712 5198
SexAI A'CCwGG_T
Cuts at: 3777 3777
Size: 6910
SfcI C'TryA_G
Cuts at: 826 1045 2663 2708 2837 4258 5393 6074 6265
Size: 219 1618 45 129 1421 1135 681 191
Cuts at: 6265 826
Size: 1471
Fragments arranged by size:
1618 1471 1421 1135 681 219 191 129
45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6910
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6910
SmaI CCC'GGG
Cuts at: 1872 3638 4237 1872
Size: 1766 599 4545
Fragments arranged by size:
4545 1766 599
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3311 3386 4100 5014 5915 6177
Size: 197 926 1368 75 714 914 901 262
Cuts at: 6177 820
Size: 1553
Fragments arranged by size:
1553 1368 926 914 901 714 262 197
75
SnaBI TAC'GTA
Cuts at: 493 4207 4701 493
Size: 3714 494 2702
Fragments arranged by size:
3714 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6910
SphI G_CATG'C
Cuts at: 1132 3676 4268 1132
Size: 2544 592 3774
Fragments arranged by size:
3774 2544 592
SspI AAT'ATT
Cuts at: 5 52 4834 5
Size: 47 4782 2081
Fragments arranged by size:
4782 2081 47
StuI AGG'CCT
Cuts at: 1517 3437 3464 1517
Size: 1920 27 4963
Fragments arranged by size:
4963 1920 27
StyI C'CwwG_G
Cuts at: 513 1065 2022 2496 2556 3022 3459 4349 513
Size: 552 957 474 60 466 437 890 3074
Fragments arranged by size:
3074 957 890 552 474 466 437 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 2978 2982 4937 4954 5107 5292
Size: 1255 579 819 4 1955 17 153 185
Cuts at: 5292 5737 325
Size: 445 1498
Fragments arranged by size:
1955 1498 1255 819 579 445 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3321 3798 5156
Size: 274 80 33 51 1443 1344 477 1358
Cuts at: 5156 6868 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1358 1344 477 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4060 4092 6870 6902 4060
Size: 32 2778 32 4068
Fragments arranged by size:
4068 2778 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6910
XbaI T'CTAG_A
Cuts at: 4246 4246
Size: 6910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3020 3640 3724 3020
Size: 620 84 6206
Fragments arranged by size:
6206 620 84
XhoI C'TCGA_G
Cuts at: 1943 3386 1943
Size: 1443 5467
XmaI C'CCGG_G
Cuts at: 1870 3636 4235 1870
Size: 1766 599 4545
Fragments arranged by size:
4545 1766 599
XmnI GAAnn'nnTTC
Cuts at: 5039 5039
Size: 6910
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 2824 3856 4718
Size: 53 83 186 936 1083 207 1032 862
Cuts at: 4718 276
Size: 2468
Fragments arranged by size:
2468 1083 1032 936 862 207 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI