Restriction map for
pFN21ASDA0704
(Circular) (Six-base) MAPSORT of: pFN21ASDA0704.seq Check: 9636 from: 1 to: 7390
pFN21ASDA0704 7390 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2757 5200 278
Size: 53 83 186 936 1221 2443 2468
Fragments arranged by size:
2468 2443 1221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2113 3343 4733 1937
Size: 176 1230 1390 4594
Fragments arranged by size:
4594 1390 1230 176
Acc65I G'GTAC_C
Cuts at: 4711 4711
Size: 7390
AclI AA'CG_TT
Cuts at: 1563 5517 5890 1563
Size: 3954 373 3063
Fragments arranged by size:
3954 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 2652 3125 3182 3396 5453 6837 7105
Size: 788 232 473 57 214 2057 1384 268
Cuts at: 7105 1632
Size: 1917
Fragments arranged by size:
2057 1917 1384 788 473 268 232 214
57
AflII C'TTAA_G
Cuts at: 820 1017 4102 820
Size: 197 3085 4108
Fragments arranged by size:
4108 3085 197
AflIII A'CryG_T
Cuts at: 1203 6289 7266 1203
Size: 5086 977 1327
Fragments arranged by size:
5086 1327 977
AgeI A'CCGG_T
Cuts at: 6221 6221
Size: 7390
AhdI GACnn_n'nnGTC
Cuts at: 6119 6119
Size: 7390
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2727 1184
Size: 1543 5847
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4245 4277 4245
Size: 32 7358
AlwNI CAG_nnn'CTG
Cuts at: 2740 5133 6705 2740
Size: 2393 1572 3425
Fragments arranged by size:
3425 2393 1572
ApaLI G'TGCA_C
Cuts at: 5447 6603 5447
Size: 1156 6234
ApoI r'AATT_y
Cuts at: 1493 2593 3952 3989 4183 4699 4925 5077 7186
Size: 1100 1359 37 194 516 226 152 2109
Cuts at: 7186 1493
Size: 1697
Fragments arranged by size:
2109 1697 1359 1100 516 226 194 152
37
AseI AT'TA_AT
Cuts at: 160 1032 5944 160
Size: 872 4912 1606
Fragments arranged by size:
4912 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
AvaI C'yCGr_G
Cuts at: 1870 1943 3583 4715 1870
Size: 73 1640 1132 4545
Fragments arranged by size:
4545 1640 1132 73
BamHI G'GATC_C
Cuts at: 4408 4720 5087 4408
Size: 312 367 6711
Fragments arranged by size:
6711 367 312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2776 3574 4622 4711 6166
Size: 325 217 627 989 798 1048 89 1455
Cuts at: 6166 618
Size: 1842
Fragments arranged by size:
1842 1455 1048 989 798 627 325 217
89
BanII G_rGCy'C
Cuts at: 1391 2331 3316 3678 3726 4709 1391
Size: 940 985 362 48 983 4072
Fragments arranged by size:
4072 985 983 940 362 48
BbeI G_GCGC'C
Cuts at: 3578 3578
Size: 7390
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2658 928
Size: 971 759 5660
Fragments arranged by size:
5660 971 759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2093 2127 2305 2339 4559 4593 5581
Size: 34 156 34 178 34 2220 34 988
Cuts at: 5581 5615 1903
Size: 34 3678
Fragments arranged by size:
3678 2220 988 178 156 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5282 6492 5282
Size: 1210 6180
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7390
BfrBI ATG'CAT
Cuts at: 2364 3803 2364
Size: 1439 5951
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6001 136
Size: 107 122 71 631 605 4329 1525
Fragments arranged by size:
4329 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3025 3199 4645 7385 3025
Size: 174 1446 2740 3030
Fragments arranged by size:
3030 2740 1446 174
BlpI GC'TnA_GC
Cuts at: 4807 4807
Size: 7390
Bme1580I G_kGCm'C
Cuts at: 1790 5451 6607 1790
Size: 3661 1156 2573
Fragments arranged by size:
3661 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2909 5121 6079
Size: 416 53 83 399 24 1492 2212 958
Cuts at: 6079 442
Size: 1753
Fragments arranged by size:
2212 1753 1492 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7390
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3310 3310
Size: 7390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3280 3731 4225 6050 1239
Size: 2041 451 494 1825 2579
Fragments arranged by size:
2579 2041 1825 494 451
Bpu10I CC'TnA_GC
Cuts at: 3147 3384 3147
Size: 237 7153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5515 6380 6678 5515
Size: 865 298 6227
Fragments arranged by size:
6227 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6053 882
Size: 617 4554 2219
Fragments arranged by size:
4554 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4687 5181 493
Size: 711 3483 494 2702
Fragments arranged by size:
3483 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 4142 7384 4142
Size: 3242 4148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2754 3575 5197 5579
Size: 53 83 186 936 1221 821 1622 382
Cuts at: 5579 6214 275
Size: 635 1451
Fragments arranged by size:
1622 1451 1221 936 821 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3841 5823 6221 6495 6642 7053 3841
Size: 1982 398 274 147 411 4178
Fragments arranged by size:
4178 1982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2071 2101 3698 3728 4125
Size: 30 165 30 668 30 1597 30 397
Cuts at: 4125 4155 1178
Size: 30 4413
Fragments arranged by size:
4413 1597 668 397 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2920 3121 3124 3233 3719 4303 1186
Size: 1210 524 201 3 109 486 584 4273
Fragments arranged by size:
4273 1210 584 524 486 201 109 3
BseYI C'CCAG_C
Cuts at: 2124 3749 6593 7089 2124
Size: 1625 2844 496 2425
Fragments arranged by size:
2844 2425 1625 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6817
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4840 5601 6629 1540
Size: 275 186 2839 761 1028 2301
Fragments arranged by size:
2839 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2084 2388 3316 3726 4128 4211 4572 5451 5536
Size: 304 928 410 402 83 361 879 85
Cuts at: 5536 6607 2084
Size: 1071 2867
Fragments arranged by size:
2867 1071 928 879 410 402 361 304
85 83
BsmI GAATG_Cn'
Cuts at: 4901 4994 4901
Size: 93 7297
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 2331 2388 3316 3678 3726 4128
Size: 399 294 247 57 928 362 48 402
Cuts at: 4128 4211 4572 4709 5451 5536 6607 1391
Size: 83 361 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 928 742 402 399 362 361
294 247 137 85 83 57 48
BspEI T'CCGG_A
Cuts at: 7053 7053
Size: 7390
BspHI T'CATG_A
Cuts at: 5279 7009 5279
Size: 1730 5660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2881 4161 4745 844
Size: 2037 1280 584 3489
Fragments arranged by size:
3489 2037 1280 584
BsrBI CCG'CTC
Cuts at: 1394 1823 5277 1394
Size: 429 3454 3507
Fragments arranged by size:
3507 3454 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2620 5885 6059 65
Size: 1183 1372 3265 174 1396
Fragments arranged by size:
3265 1396 1372 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3571 3658 6034 6221 1700
Size: 110 1761 87 2376 187 2869
Fragments arranged by size:
2869 2376 1761 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7390
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7390
BssSI C'ACGA_G
Cuts at: 2643 5450 6462 2643
Size: 2807 1012 3571
Fragments arranged by size:
3571 2807 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3785 794
Size: 2991 4399
BstBI TT'CG_AA
Cuts at: 2007 6194 2007
Size: 4187 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7390
BstXI CCAn_nnnn'nTGG
Cuts at: 2698 3661 3757 2698
Size: 963 96 6331
Fragments arranged by size:
6331 963 96
BstYI r'GATC_y
Cuts at: 1972 2804 3025 3199 4408 4645 4720 5087 5472
Size: 832 221 174 1209 237 75 367 385
Cuts at: 5472 5489 6941 7027 7039 7385 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1209 832 385 367 346 237
221 174 86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 3617 3617
Size: 7390
BtgI C'CryG_G
Cuts at: 513 1065 4005 513
Size: 552 2940 3898
Fragments arranged by size:
3898 2940 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6651
Fragments arranged by size:
6651 683 56
BtsI GCAGTG_nn'
Cuts at: 2885 4906 5700 5720 2885
Size: 2021 794 20 4555
Fragments arranged by size:
4555 2021 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7355
DraI TTT'AAA
Cuts at: 4694 4694
Size: 7390
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3443 4673 6397 809
Size: 507 2127 1230 1724 1802
Fragments arranged by size:
2127 1802 1724 1230 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3651 4837 5726
Size: 54 1425 50 275 18 1821 1186 889
Cuts at: 5726 7355 8
Size: 1629 43
Fragments arranged by size:
1821 1629 1425 1186 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4837 1537
Size: 275 3025 4090
Fragments arranged by size:
4090 3025 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2101 2539 2961 3415 4321 5320 1353
Size: 748 438 422 454 906 999 3423
Fragments arranged by size:
3423 999 906 748 454 438 422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3169 3909 5977 6351 6497 387
Size: 1064 138 1580 740 2068 374 146 1280
Fragments arranged by size:
2068 1580 1280 1064 740 374 146 138
EcoICRI GAG'CTC
Cuts at: 3314 3724 3314
Size: 410 6980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2652 3125 3182 3280 3396 3731
Size: 393 788 232 473 57 98 116 335
Cuts at: 3731 4225 5453 6050 6837 7105 1239
Size: 494 1228 597 787 268 1524
Fragments arranged by size:
1524 1228 788 787 597 494 473 393
335 268 232 116 98 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3644 3731 3644
Size: 87 7303
EcoO109I rG'GnC_Cy
Cuts at: 2893 3518 3637 2893
Size: 625 119 6646
Fragments arranged by size:
6646 625 119
EcoRI G'AATT_C
Cuts at: 4699 5077 4699
Size: 378 7012
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2257 3087 2233
Size: 24 830 6536
Fragments arranged by size:
6536 830 24
FspI TGC'GCA
Cuts at: 5896 5896
Size: 7390
HaeII r_GCGC'y
Cuts at: 1678 3578 6537 1678
Size: 1900 2959 2531
Fragments arranged by size:
2959 2531 1900
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 2522 2554 3116
Size: 32 135 32 848 32 70 32 562
Cuts at: 3116 3148 3336 3368 3422 3454 4125 4157 4324
Size: 32 188 32 54 32 671 32 167
Cuts at: 4324 4356 4400 4432 4589 4621 6031 6063 6105
Size: 32 44 32 157 32 1410 32 42
Cuts at: 6105 6137 1373
Size: 32 2626
Fragments arranged by size:
2626 1410 848 671 562 188 167 157
135 70 54 44 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2114 2500 2949 4734 4980 669
Size: 988 281 176 386 449 1785 246 3079
Fragments arranged by size:
3079 1785 988 449 386 281 246 176
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7390
HpaI GTT'AAC
Cuts at: 2500 4980 2500
Size: 2480 4910
KasI G'GCGC_C
Cuts at: 3574 3574
Size: 7390
KpnI G_GTAC'C
Cuts at: 4715 4715
Size: 7390
MfeI C'AATT_G
Cuts at: 4020 4989 4020
Size: 969 6421
MluI A'CGCG_T
Cuts at: 7266 7266
Size: 7390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4620 6504 6688 1305
Size: 3315 1884 184 2007
Fragments arranged by size:
3315 2007 1884 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3653 10
Size: 54 1425 343 1821 3747
Fragments arranged by size:
3747 1821 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2359 2709 3659 3862 5348 5707 5866
Size: 648 1193 350 950 203 1486 359 159
Cuts at: 5866 518
Size: 2042
Fragments arranged by size:
2042 1486 1193 950 648 359 350 203
159
MspA1I CmG'CkG
Cuts at: 1606 2124 3089 3112 3257 4806 5483 6631 6876
Size: 518 965 23 145 1549 677 1148 245
Cuts at: 6876 1606
Size: 2120
Fragments arranged by size:
2120 1549 1148 965 677 518 245 145
23
NaeI GCC'GGC
Cuts at: 3573 3573
Size: 7390
NarI GG'CG_CC
Cuts at: 3575 3575
Size: 7390
NcoI C'CATG_G
Cuts at: 513 1065 4005 513
Size: 552 2940 3898
Fragments arranged by size:
3898 2940 552
NdeI CA'TA_TG
Cuts at: 387 3442 3859 387
Size: 3055 417 3918
Fragments arranged by size:
3918 3055 417
NgoMIV G'CCGG_C
Cuts at: 3571 3571
Size: 7390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7390
NotI GC'GGCC_GC
Cuts at: 4837 4837
Size: 7390
NsiI A_TGCA'T
Cuts at: 2366 3805 2366
Size: 1439 5951
NspI r_CATG'y
Cuts at: 1132 1229 4748 6293 1132
Size: 97 3519 1545 2229
Fragments arranged by size:
3519 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7390
PciI A'CATG_T
Cuts at: 6289 6289
Size: 7390
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2062 4052 1108
Size: 954 1990 4446
Fragments arranged by size:
4446 1990 954
PfoI T'CCnGG_A
Cuts at: 3551 3726 3551
Size: 175 7215
PmeI GTTT'AAAC
Cuts at: 4694 4694
Size: 7390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3303 3335 5459 5491 6972 7004 3303
Size: 32 2124 32 1481 32 3689
Fragments arranged by size:
3689 2124 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2893 3637 2893
Size: 744 6646
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7390
PsiI TTA'TAA
Cuts at: 4190 4960 4190
Size: 770 6620
PstI C_TGCA'G
Cuts at: 830 2892 3693 4530 4742 830
Size: 2062 801 837 212 3478
Fragments arranged by size:
3478 2062 837 801 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7390
PvuII CAG'CTG
Cuts at: 2124 3112 2124
Size: 988 6402
SacI G_AGCT'C
Cuts at: 3316 3726 3316
Size: 410 6980
SalI G'TCGA_C
Cuts at: 1936 2112 4732 1936
Size: 176 2620 4594
Fragments arranged by size:
4594 2620 176
SapI GCTCTTCn'nnn_
Cuts at: 2101 3415 2101
Size: 1314 6076
SbfI CC_TGCA'GG
Cuts at: 4742 4742
Size: 7390
ScaI AGT'ACT
Cuts at: 5638 7350 5638
Size: 1712 5678
SexAI A'CCwGG_T
Cuts at: 2718 2718
Size: 7390
SfcI C'TryA_G
Cuts at: 826 1045 2888 3689 4369 4526 4738 5873 6554
Size: 219 1843 801 680 157 212 1135 681
Cuts at: 6554 6745 826
Size: 191 1471
Fragments arranged by size:
1843 1471 1135 801 681 680 219 212
191 157
SfoI GGC'GCC
Cuts at: 3576 3576
Size: 7390
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7390
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7390
SmaI CCC'GGG
Cuts at: 1872 4717 1872
Size: 2845 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4102 5494 6395 6657 820
Size: 197 926 2159 1392 901 262 1553
Fragments arranged by size:
2159 1553 1392 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4687 5181 493
Size: 4194 494 2702
Fragments arranged by size:
4194 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7390
SphI G_CATG'C
Cuts at: 1132 4748 1132
Size: 3616 3774
SspI AAT'ATT
Cuts at: 5 52 5314 5
Size: 47 5262 2081
Fragments arranged by size:
5262 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7390
StyI C'CwwG_G
Cuts at: 513 1065 2044 3513 3645 4005 4829 513
Size: 552 979 1469 132 360 824 3074
Fragments arranged by size:
3074 1469 979 824 552 360 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 4600 5417 5434 5587 5772 6217
Size: 1255 600 2420 817 17 153 185 445
Cuts at: 6217 325
Size: 1498
Fragments arranged by size:
2420 1498 1255 817 600 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3714 5636 7348
Size: 274 80 33 51 1443 1737 1922 1712
Cuts at: 7348 96
Size: 138
Fragments arranged by size:
1922 1737 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2877 2909 7350 7382 2877
Size: 32 4441 32 2885
Fragments arranged by size:
4441 2885 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7390
XbaI T'CTAG_A
Cuts at: 3947 4471 4726 3947
Size: 524 255 6611
Fragments arranged by size:
6611 524 255
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7390
XmaI C'CCGG_G
Cuts at: 1870 4715 1870
Size: 2845 4545
XmnI GAAnn'nnTTC
Cuts at: 3867 4014 5519 3867
Size: 147 1505 5738
Fragments arranged by size:
5738 1505 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2755 5198 276
Size: 53 83 186 936 1221 2443 2468
Fragments arranged by size:
2468 2443 1221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI XcmI