Restriction map for
pFN21ASDA0694
(Circular) (Six-base) MAPSORT of: pFN21ASDA0694.seq Check: 4930 from: 1 to: 6508
pFN21ASDA0694 6508 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3851 1937
Size: 1914 4594
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 3778 4635 5008 1563
Size: 2215 857 373 3063
Fragments arranged by size:
3063 2215 857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 2991 3221 3751 4571 5955 6223 1632
Size: 702 657 230 530 820 1384 268 1917
Fragments arranged by size:
1917 1384 820 702 657 530 268 230
AfeI AGC'GCT
Cuts at: 2648 2648
Size: 6508
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 5407 6384 1203
Size: 4204 977 1327
Fragments arranged by size:
4204 1327 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6508
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 6476
AlwNI CAG_nnn'CTG
Cuts at: 2312 4251 5823 2312
Size: 1939 1572 2997
Fragments arranged by size:
2997 1939 1572
ApaI G_GGCC'C
Cuts at: 2910 2910
Size: 6508
ApaLI G'TGCA_C
Cuts at: 4565 5721 4565
Size: 1156 5352
ApoI r'AATT_y
Cuts at: 1493 2338 2363 2596 3488 3817 4043 4195 6304
Size: 845 25 233 892 329 226 152 2109
Cuts at: 6304 1493
Size: 1697
Fragments arranged by size:
2109 1697 892 845 329 233 226 152
25
AseI AT'TA_AT
Cuts at: 160 1032 5062 160
Size: 872 4030 1606
Fragments arranged by size:
4030 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2436 3833 1870
Size: 73 222 271 1397 4545
Fragments arranged by size:
4545 1397 271 222 73
AvrII C'CTAG_G
Cuts at: 2156 2156
Size: 6508
BamHI G'GATC_C
Cuts at: 2865 3838 4205 2865
Size: 973 367 5168
Fragments arranged by size:
5168 973 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3587 3829 5284 618
Size: 325 217 627 1800 242 1455 1842
Fragments arranged by size:
1842 1800 1455 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 2088 2910 2937 3827 1391
Size: 697 822 27 890 4072
Fragments arranged by size:
4072 890 822 697 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2523 928
Size: 971 624 4913
Fragments arranged by size:
4913 971 624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 3168 3207 1548
Size: 1620 39 4849
Fragments arranged by size:
4849 1620 39
BfrBI ATG'CAT
Cuts at: 2736 2736
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2117 5119 136
Size: 107 122 71 631 605 445 3002 1525
Fragments arranged by size:
3002 1525 631 605 445 122 107 71
BglII A'GATC_T
Cuts at: 2233 3145 6503 2233
Size: 912 3358 2238
Fragments arranged by size:
3358 2238 912
BlpI GC'TnA_GC
Cuts at: 3925 3925
Size: 6508
Bme1580I G_kGCm'C
Cuts at: 1790 2910 4569 5725 1790
Size: 1120 1659 1156 2573
Fragments arranged by size:
2573 1659 1156 1120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2244 2371 4239
Size: 416 53 83 399 24 827 127 1868
Cuts at: 4239 5197 442
Size: 958 1753
Fragments arranged by size:
1868 1753 958 827 416 399 127 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2436 5168 1239
Size: 1197 2732 2579
Fragments arranged by size:
2732 2579 1197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4633 5498 5796 4633
Size: 865 298 5345
Fragments arranged by size:
5345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2059 5171 882
Size: 617 560 3112 2219
Fragments arranged by size:
3112 2219 617 560
BsaAI yAC'GTr
Cuts at: 493 1204 3805 4299 493
Size: 711 2601 494 2702
Fragments arranged by size:
2702 2601 711 494
BsaBI GATnn'nnATC
Cuts at: 6502 6502
Size: 6508
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4315 4697 5332 275
Size: 53 83 186 936 2782 382 635 1451
Fragments arranged by size:
2782 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2214 4941 5339 5613 5760 6171 2214
Size: 2727 398 274 147 411 2551
Fragments arranged by size:
2727 2551 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3418 3448 3599 3629 1178
Size: 30 165 30 2015 30 151 30 4057
Fragments arranged by size:
4057 2015 165 151 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2562 2935 2938 1186
Size: 1376 373 3 4756
Fragments arranged by size:
4756 1376 373 3
BseYI C'CCAG_C
Cuts at: 5711 6207 5711
Size: 496 6012
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2786 1163
Size: 573 1050 4885
Fragments arranged by size:
4885 1050 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 3958 4719 5747 1540
Size: 275 186 81 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 2088 2328 2930 2937 4569 4654 5725 2088
Size: 240 602 7 1632 85 1071 2871
Fragments arranged by size:
2871 1632 1071 602 240 85 7
BsmI GAATG_Cn'
Cuts at: 2883 2987 4019 4112 2883
Size: 104 1032 93 5279
Fragments arranged by size:
5279 1032 104 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2328 2910 2930 2937 3827 4569
Size: 399 298 240 582 20 7 890 742
Cuts at: 4569 4654 5725 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 890 742 582 399 298 240
85 20 7
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 3204 4397 6127 3204
Size: 1193 1730 3585
Fragments arranged by size:
3585 1730 1193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 3863 844
Size: 1190 1829 3489
Fragments arranged by size:
3489 1829 1190
BsrBI CCG'CTC
Cuts at: 1394 1823 2035 2552 4395 1394
Size: 429 212 517 1843 3507
Fragments arranged by size:
3507 1843 517 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 3576 5003 5177 65
Size: 1183 2328 1427 174 1396
Fragments arranged by size:
2328 1427 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2130 5152 5339 1700
Size: 110 217 103 3022 187 2869
Fragments arranged by size:
3022 2869 217 187 110 103
BsrGI T'GTAC_A
Cuts at: 96 2753 96
Size: 2657 3851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 4568 5580 4568
Size: 1012 5496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3032 3587 794
Size: 2238 555 3715
Fragments arranged by size:
3715 2238 555
BstBI TT'CG_AA
Cuts at: 2007 5312 2007
Size: 3305 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6508
BstXI CCAn_nnnn'nTGG
Cuts at: 3480 3480
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2233 2865 3109 3145 3355 3838 4205 4590
Size: 261 632 244 36 210 483 367 385
Cuts at: 4590 4607 6059 6145 6157 6503 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 632 483 385 367 346 261
244 210 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2901 2901
Size: 6508
BtgI C'CryG_G
Cuts at: 513 1065 2022 2112 513
Size: 552 957 90 4909
Fragments arranged by size:
4909 957 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 4024 4818 4838 4024
Size: 794 20 5694
Fragments arranged by size:
5694 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2524 2559 529
Size: 35 1960 35 4478
Fragments arranged by size:
4478 1960 35 35
DraI TTT'AAA
Cuts at: 3812 3812
Size: 6508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2079 3546
Size: 54 1425 50 275 18 195 54 1467
Cuts at: 3546 3955 4844 6473 8
Size: 409 889 1629 43
Fragments arranged by size:
1629 1467 1425 889 409 275 195 54
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 3955 1537
Size: 275 267 1876 4090
Fragments arranged by size:
4090 1876 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 4438 1353
Size: 879 2206 3423
Fragments arranged by size:
3423 2206 879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2095 5095 5469 5615 387
Size: 1064 138 506 3000 374 146 1280
Fragments arranged by size:
3000 1280 1064 506 374 146 138
EcoICRI GAG'CTC
Cuts at: 2086 2935 2086
Size: 849 5659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2436 2991 3221 3751 4571 5168
Size: 393 702 102 555 230 530 820 597
Cuts at: 5168 5955 6223 1239
Size: 787 268 1524
Fragments arranged by size:
1524 820 787 702 597 555 530 393
268 230 102
EcoO109I rG'GnC_Cy
Cuts at: 2906 2907 2906
Size: 1 6507
EcoRI G'AATT_C
Cuts at: 3817 4195 3817
Size: 378 6130
EcoRV GAT'ATC
Cuts at: 2287 2287
Size: 6508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 6508
FspI TGC'GCA
Cuts at: 5014 5014
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 2100 2650 5655 1678
Size: 422 550 3005 2531
Fragments arranged by size:
3005 2531 550 422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 3079 3111 3369
Size: 32 135 32 1127 32 348 32 258
Cuts at: 3369 3401 5149 5181 5223 5255 1373
Size: 32 1748 32 42 32 2626
Fragments arranged by size:
2626 1748 1127 348 258 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3715 3852 4098 669
Size: 988 281 1777 137 246 3079
Fragments arranged by size:
3079 1777 988 281 246 137
HindIII A'AGCT_T
Cuts at: 748 2459 3763 748
Size: 1711 1304 3493
Fragments arranged by size:
3493 1711 1304
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 4107 4107
Size: 6508
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3642 3672 5622 5806 1305
Size: 2337 30 1950 184 2007
Fragments arranged by size:
2337 2007 1950 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3548 10
Size: 54 1425 343 1716 2970
Fragments arranged by size:
2970 1716 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2493 4466 4825 4984 518
Size: 648 1327 1973 359 159 2042
Fragments arranged by size:
2042 1973 1327 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2114 2143 2659 2837 3924 4601 5749 5994
Size: 508 29 516 178 1087 677 1148 245
Cuts at: 5994 1606
Size: 2120
Fragments arranged by size:
2120 1148 1087 677 516 508 245 178
29
NaeI GCC'GGC
Cuts at: 2132 2132
Size: 6508
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4999
Fragments arranged by size:
4999 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NgoMIV G'CCGG_C
Cuts at: 2130 2130
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NsiI A_TGCA'T
Cuts at: 2738 2738
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 2387 3866 5411 1132
Size: 97 1158 1479 1545 2229
Fragments arranged by size:
2229 1545 1479 1158 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6508
PciI A'CATG_T
Cuts at: 5407 5407
Size: 6508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6508
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3599 3631 4577 4609 6090 6122 3599
Size: 32 946 32 1481 32 3985
Fragments arranged by size:
3985 1481 946 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6508
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PspOMI G'GGCC_C
Cuts at: 2906 2906
Size: 6508
PstI C_TGCA'G
Cuts at: 830 3665 3860 830
Size: 2835 195 3478
Fragments arranged by size:
3478 2835 195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6508
PvuII CAG'CTG
Cuts at: 2659 2837 2659
Size: 178 6330
SacI G_AGCT'C
Cuts at: 2088 2937 2088
Size: 849 5659
SacII CC_GC'GG
Cuts at: 2115 2115
Size: 6508
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 4756 6468 4756
Size: 1712 4796
SfcI C'TryA_G
Cuts at: 826 1045 3661 3856 4991 5672 5863 826
Size: 219 2616 195 1135 681 191 1471
Fragments arranged by size:
2616 1471 1135 681 219 195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3835 1872
Size: 1963 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 4612 5513 5775 820
Size: 197 926 222 2447 901 262 1553
Fragments arranged by size:
2447 1553 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 3805 4299 493
Size: 3312 494 2702
Fragments arranged by size:
3312 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 2411 3280 4432 5
Size: 47 2359 869 1152 2081
Fragments arranged by size:
2359 2081 1152 869 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6508
StyI C'CwwG_G
Cuts at: 513 1065 2022 2156 2535 2914 3947 513
Size: 552 957 134 379 379 1033 3074
Fragments arranged by size:
3074 1033 957 552 379 379 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2622 4535 4552 4705 4890 5335 325
Size: 1255 1042 1913 17 153 185 445 1498
Fragments arranged by size:
1913 1498 1255 1042 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 2753 4754
Size: 274 80 33 51 1443 321 455 2001
Cuts at: 4754 6466 96
Size: 1712 138
Fragments arranged by size:
2001 1712 1443 455 321 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2516 2548 3418 3450 6468 6500 2516
Size: 32 870 32 3018 32 2524
Fragments arranged by size:
3018 2524 870 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6508
XbaI T'CTAG_A
Cuts at: 3106 3844 3106
Size: 738 5770
XhoI C'TCGA_G
Cuts at: 1943 2165 1943
Size: 222 6286
XmaI C'CCGG_G
Cuts at: 1870 3833 1870
Size: 1963 4545
XmnI GAAnn'nnTTC
Cuts at: 4637 4637
Size: 6508
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NarI
NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI