Restriction map for
pFN21ASDA0688
(Circular) (Six-base) MAPSORT of: pFN21ASDA0688.seq Check: 6148 from: 1 to: 7240
pFN21ASDA0688 7240 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3896 3896
Size: 7240
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5050 278
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4583 1937
Size: 2646 4594
Acc65I G'GTAC_C
Cuts at: 4561 4561
Size: 7240
AclI AA'CG_TT
Cuts at: 1563 5367 5740 1563
Size: 3804 373 3063
Fragments arranged by size:
3804 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4081 4229 5303 6687 6955 1632
Size: 2449 148 1074 1384 268 1917
Fragments arranged by size:
2449 1917 1384 1074 268 148
AfeI AGC'GCT
Cuts at: 2657 2741 2657
Size: 84 7156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7043
AflIII A'CryG_T
Cuts at: 1203 6139 7116 1203
Size: 4936 977 1327
Fragments arranged by size:
4936 1327 977
AgeI A'CCGG_T
Cuts at: 6071 6071
Size: 7240
AhdI GACnn_n'nnGTC
Cuts at: 5969 5969
Size: 7240
AleI CACnn'nnGTG
Cuts at: 1166 3857 4271 1166
Size: 2691 414 4135
Fragments arranged by size:
4135 2691 414
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2252 3538 3801 1184
Size: 1068 1286 263 4623
Fragments arranged by size:
4623 1286 1068 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 3937 3969 2490
Size: 32 1415 32 5761
Fragments arranged by size:
5761 1415 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2356 2393 2515 2887 2898 2983 4495 4983 6555
Size: 37 122 372 11 85 1512 488 1572
Cuts at: 6555 2356
Size: 3041
Fragments arranged by size:
3041 1572 1512 488 372 122 85 37
11
ApaI G_GGCC'C
Cuts at: 2859 2871 3387 3513 3831 2859
Size: 12 516 126 318 6268
Fragments arranged by size:
6268 516 318 126 12
ApaLI G'TGCA_C
Cuts at: 5297 6453 5297
Size: 1156 6084
ApoI r'AATT_y
Cuts at: 1493 4103 4549 4775 4927 7036 1493
Size: 2610 446 226 152 2109 1697
Fragments arranged by size:
2610 2109 1697 446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5794 160
Size: 872 4762 1606
Fragments arranged by size:
4762 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2190 2245 3654 3897 4565 1870
Size: 73 102 145 55 1409 243 668 4545
Fragments arranged by size:
4545 1409 668 243 145 102 73 55
BamHI G'GATC_C
Cuts at: 3217 4570 4937 3217
Size: 1353 367 5520
Fragments arranged by size:
5520 1353 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2365 2396 2411 2525
Size: 325 217 627 462 116 31 15 114
Cuts at: 2525 3029 4512 4561 6016 618
Size: 504 1483 49 1455 1842
Fragments arranged by size:
1842 1483 1455 627 504 462 325 217
116 114 49 31 15
BanII G_rGCy'C
Cuts at: 1391 2083 2467 2557 2859 2871 3081 3231 3387
Size: 692 384 90 302 12 210 150 156
Cuts at: 3387 3513 3831 4559 1391
Size: 126 318 728 4072
Fragments arranged by size:
4072 728 692 384 318 302 210 156
150 126 90 12
BbeI G_GCGC'C
Cuts at: 2369 4516 2369
Size: 2147 5093
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3112 928
Size: 971 1213 5056
Fragments arranged by size:
5056 1213 971
BbvCI CC'TCA_GC
Cuts at: 2164 2164
Size: 7240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5431 5465 1903
Size: 34 3494 34 3678
Fragments arranged by size:
3678 3494 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5132 6342 4109
Size: 1023 1210 5007
Fragments arranged by size:
5007 1210 1023
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7240
BfrBI ATG'CAT
Cuts at: 3153 3993 3153
Size: 840 6400
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 3750 5851
Size: 107 122 71 631 605 502 1576 2101
Cuts at: 5851 136
Size: 1525
Fragments arranged by size:
2101 1576 1525 631 605 502 122 107
71
BglII A'GATC_T
Cuts at: 7235 7235
Size: 7240
BlpI GC'TnA_GC
Cuts at: 3754 4657 3754
Size: 903 6337
Bme1580I G_kGCm'C
Cuts at: 1790 2252 2859 2871 3032 3250 3288 3387 3513
Size: 462 607 12 161 218 38 99 126
Cuts at: 3513 3831 3904 4431 5301 6457 1790
Size: 318 73 527 870 1156 2573
Fragments arranged by size:
2573 1156 870 607 527 462 318 218
161 126 99 73 38 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 2865 3119
Size: 416 53 83 399 24 1044 404 254
Cuts at: 3119 3847 3914 4971 5929 442
Size: 728 67 1057 958 1753
Fragments arranged by size:
1753 1057 1044 958 728 416 404 399
254 83 67 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 3198 2335
Size: 863 6377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2492 2537 3065 3542 3944 4517 5900
Size: 1091 162 45 528 477 402 573 1383
Cuts at: 5900 1239
Size: 2579
Fragments arranged by size:
2579 1383 1091 573 528 477 402 162
45
Bpu10I CC'TnA_GC
Cuts at: 2164 2370 2164
Size: 206 7034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5365 6230 6528 5365
Size: 865 298 6077
Fragments arranged by size:
6077 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5903 882
Size: 617 4404 2219
Fragments arranged by size:
4404 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4537 5031 493
Size: 711 3333 494 2702
Fragments arranged by size:
3333 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7234 7234
Size: 7240
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2366 4513 5047 5429
Size: 53 83 186 936 833 2147 534 382
Cuts at: 5429 6064 275
Size: 635 1451
Fragments arranged by size:
2147 1451 936 833 635 534 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2321 2428 4188 5673 6071 6345 6492 6903 2321
Size: 107 1760 1485 398 274 147 411 2658
Fragments arranged by size:
2658 1760 1485 411 398 274 147 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2076 2106 2192 2222 2490
Size: 30 165 30 673 30 86 30 268
Cuts at: 2490 2520 3218 3248 1178
Size: 30 698 30 5170
Fragments arranged by size:
5170 698 673 268 165 86 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2213 2607 2864 3239 3640 3788 3958
Size: 1024 3 394 257 375 401 148 170
Cuts at: 3958 1186
Size: 4468
Fragments arranged by size:
4468 1024 401 394 375 257 170 148
3
BseYI C'CCAG_C
Cuts at: 2377 2540 2630 3010 3588 4356 6443 6939 2377
Size: 163 90 380 578 768 2087 496 2678
Fragments arranged by size:
2678 2087 768 578 496 380 163 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 2369 2432 3646 4460 1163
Size: 573 612 21 63 1214 814 3943
Fragments arranged by size:
3943 1214 814 612 573 63 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4436 4690 5451 6479 1540
Size: 275 186 2435 254 761 1028 2301
Fragments arranged by size:
2435 2301 1028 761 275 254 186
BsiHKAI G_wGCw'C
Cuts at: 3179 3440 4489 5301 5386 6457 3179
Size: 261 1049 812 85 1071 3962
Fragments arranged by size:
3962 1071 1049 812 261 85
BsmI GAATG_Cn'
Cuts at: 2717 4751 4844 2717
Size: 2034 93 5113
Fragments arranged by size:
5113 2034 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2285 2285
Size: 7240
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2252 2467 2557 2859 2871 3032
Size: 399 293 169 215 90 302 12 161
Cuts at: 3032 3081 3179 3231 3250 3288 3387 3440 3513
Size: 49 98 52 19 38 99 53 73
Cuts at: 3513 3831 3904 4431 4489 4559 5301 5386 6457
Size: 318 73 527 58 70 742 85 1071
Cuts at: 6457 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 527 399 318 302 293
215 169 161 99 98 90 85 73
73 70 58 53 52 49 38 19
12
BspEI T'CCGG_A
Cuts at: 2428 6903 2428
Size: 4475 2765
BspHI T'CATG_A
Cuts at: 3103 5129 6859 3103
Size: 2026 1730 3484
Fragments arranged by size:
3484 2026 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 2754 3896 4595 844
Size: 1476 434 1142 699 3489
Fragments arranged by size:
3489 1476 1142 699 434
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 3723 5127 1394
Size: 429 290 1610 1404 3507
Fragments arranged by size:
3507 1610 1404 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 3314 5735 5909 65
Size: 1183 2066 2421 174 1396
Fragments arranged by size:
2421 2066 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5884 6071 1700
Size: 110 4074 187 2869
Fragments arranged by size:
4074 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7240
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7240
BssSI C'ACGA_G
Cuts at: 3664 5300 6312 3664
Size: 1636 1012 4592
Fragments arranged by size:
4592 1636 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3504 4053 794
Size: 2710 549 3981
Fragments arranged by size:
3981 2710 549
BstBI TT'CG_AA
Cuts at: 2007 6044 2007
Size: 4037 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7240
BstXI CCAn_nnnn'nTGG
Cuts at: 3452 4273 3452
Size: 821 6419
BstYI r'GATC_y
Cuts at: 1972 2425 3217 4500 4570 4937 5322 5339 6791
Size: 453 792 1283 70 367 385 17 1452
Cuts at: 6791 6877 6889 7235 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1283 792 453 385 367 346
86 70 17 12
Bsu36I CC'TnA_GG
Cuts at: 2928 3222 2928
Size: 294 6946
BtgI C'CryG_G
Cuts at: 513 1065 2722 3605 3831 3929 4140 4342 513
Size: 552 1657 883 226 98 211 202 3411
Fragments arranged by size:
3411 1657 883 552 226 211 202 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6501
Fragments arranged by size:
6501 683 56
BtsI GCAGTG_nn'
Cuts at: 3093 3250 3785 4314 4367 4756 5550 5570 3093
Size: 157 535 529 53 389 794 20 4763
Fragments arranged by size:
4763 794 535 529 389 157 53 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7240
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7205
DraI TTT'AAA
Cuts at: 4544 4544
Size: 7240
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6247 809
Size: 507 4931 1802
Fragments arranged by size:
4931 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2712 3442
Size: 54 1425 50 275 18 345 537 730
Cuts at: 3442 3454 4183 4433 4687 5576 7205 8
Size: 12 729 250 254 889 1629 43
Fragments arranged by size:
1629 1425 889 730 729 537 345 275
254 250 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 4433 4687 1537
Size: 275 2621 254 4090
Fragments arranged by size:
4090 2621 275 254
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 2641 3815 4477 5170 1353
Size: 1214 74 1174 662 693 3423
Fragments arranged by size:
3423 1214 1174 693 662 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5827 6201 6347 387
Size: 1064 138 4238 374 146 1280
Fragments arranged by size:
4238 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2492 2537 3065 3542 3944 4081
Size: 393 698 162 45 528 477 402 137
Cuts at: 4081 4229 4517 5303 5900 6687 6955 1239
Size: 148 288 786 597 787 268 1524
Fragments arranged by size:
1524 787 786 698 597 528 477 402
393 288 268 162 148 137 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 7240
EcoO109I rG'GnC_Cy
Cuts at: 2546 2594 2855 2856 2867 2914 2980 3593 3827
Size: 48 261 1 11 47 66 613 234
Cuts at: 3827 3945 2546
Size: 118 5841
Fragments arranged by size:
5841 613 261 234 118 66 48 47
11 1
EcoRI G'AATT_C
Cuts at: 4549 4927 4549
Size: 378 6862
FspI TGC'GCA
Cuts at: 2891 5746 2891
Size: 2855 4385
HaeII r_GCGC'y
Cuts at: 1678 2068 2369 2659 2743 4516 6387 1678
Size: 390 301 290 84 1773 1871 2531
Fragments arranged by size:
2531 1871 1773 390 301 290 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 3218 3250 3953
Size: 32 135 32 845 32 769 32 703
Cuts at: 3953 3985 4319 4351 5881 5913 5955 5987 1373
Size: 32 334 32 1530 32 42 32 2626
Fragments arranged by size:
2626 1530 845 769 703 334 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4584 4830 669
Size: 988 281 2646 246 3079
Fragments arranged by size:
3079 2646 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7240
HpaI GTT'AAC
Cuts at: 4830 4830
Size: 7240
KasI G'GCGC_C
Cuts at: 2365 4512 2365
Size: 2147 5093
KpnI G_GTAC'C
Cuts at: 4565 4565
Size: 7240
MfeI C'AATT_G
Cuts at: 4839 4839
Size: 7240
MluI A'CGCG_T
Cuts at: 7116 7116
Size: 7240
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6354 6538 1305
Size: 5049 184 2007
Fragments arranged by size:
5049 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 3444 3456 4185 10
Size: 54 1425 343 345 1267 12 729 3065
Fragments arranged by size:
3065 1425 1267 729 345 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2454 3450 3857 4253 4271 5198 5557
Size: 648 1288 996 407 396 18 927 359
Cuts at: 5557 5716 518
Size: 159 2042
Fragments arranged by size:
2042 1288 996 927 648 407 396 359
159 18
MspA1I CmG'CkG
Cuts at: 1606 2586 2911 3373 3409 4142 4308 4656 5333
Size: 980 325 462 36 733 166 348 677
Cuts at: 5333 6481 6726 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 980 733 677 462 348 325
245 166 36
NarI GG'CG_CC
Cuts at: 2366 4513 2366
Size: 2147 5093
NcoI C'CATG_G
Cuts at: 513 1065 3605 3831 4342 513
Size: 552 2540 226 511 3411
Fragments arranged by size:
3411 2540 552 511 226
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7240
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7240
NotI GC'GGCC_GC
Cuts at: 4687 4687
Size: 7240
NsiI A_TGCA'T
Cuts at: 3155 3995 3155
Size: 840 6400
NspI r_CATG'y
Cuts at: 1132 1229 3130 4598 6143 1132
Size: 97 1901 1468 1545 2229
Fragments arranged by size:
2229 1901 1545 1468 97
PasI CC'CwG_GG
Cuts at: 1348 3486 3824 4518 1348
Size: 2138 338 694 4070
Fragments arranged by size:
4070 2138 694 338
PciI A'CATG_T
Cuts at: 6139 6139
Size: 7240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2515 3193 3581 3837 1108
Size: 1407 678 388 256 4511
Fragments arranged by size:
4511 1407 678 388 256
PfoI T'CCnGG_A
Cuts at: 3267 3558 3267
Size: 291 6949
PmeI GTTT'AAAC
Cuts at: 4544 4544
Size: 7240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2490 2522 5309 5341 6822 6854 2490
Size: 32 2787 32 1481 32 2876
Fragments arranged by size:
2876 2787 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2546 2594 2980 3593 3945 2546
Size: 48 386 613 352 5841
Fragments arranged by size:
5841 613 386 352 48
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7240
PsiI TTA'TAA
Cuts at: 4810 4810
Size: 7240
PspOMI G'GGCC_C
Cuts at: 2855 2867 3383 3509 3827 2855
Size: 12 516 126 318 6268
Fragments arranged by size:
6268 516 318 126 12
PstI C_TGCA'G
Cuts at: 830 3245 4321 4592 830
Size: 2415 1076 271 3478
Fragments arranged by size:
3478 2415 1076 271
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7240
PvuII CAG'CTG
Cuts at: 3373 3409 3373
Size: 36 7204
SacII CC_GC'GG
Cuts at: 4143 4143
Size: 7240
SalI G'TCGA_C
Cuts at: 1936 4582 1936
Size: 2646 4594
SanDI GG'GwC_CC
Cuts at: 3945 3945
Size: 7240
SapI GCTCTTCn'nnn_
Cuts at: 2641 4477 2641
Size: 1836 5404
SbfI CC_TGCA'GG
Cuts at: 4592 4592
Size: 7240
ScaI AGT'ACT
Cuts at: 3695 5488 7200 3695
Size: 1793 1712 3735
Fragments arranged by size:
3735 1793 1712
SfcI C'TryA_G
Cuts at: 826 1045 3241 4303 4317 4588 5723 6404 6595
Size: 219 2196 1062 14 271 1135 681 191
Cuts at: 6595 826
Size: 1471
Fragments arranged by size:
2196 1471 1135 1062 681 271 219 191
14
SfoI GGC'GCC
Cuts at: 2367 4514 2367
Size: 2147 5093
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7240
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7240
SmaI CCC'GGG
Cuts at: 1872 2047 2192 3899 4567 1872
Size: 175 145 1707 668 4545
Fragments arranged by size:
4545 1707 668 175 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5344 6245 6507 820
Size: 197 926 3401 901 262 1553
Fragments arranged by size:
3401 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4537 5031 493
Size: 4044 494 2702
Fragments arranged by size:
4044 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7240
SphI G_CATG'C
Cuts at: 1132 4598 1132
Size: 3466 3774
SrfI GCCC'GGGC
Cuts at: 3899 3899
Size: 7240
SspI AAT'ATT
Cuts at: 5 52 3571 5164 5
Size: 47 3519 1593 2081
Fragments arranged by size:
3519 2081 1593 47
StuI AGG'CCT
Cuts at: 1517 2282 2777 3521 3670 1517
Size: 765 495 744 149 5087
Fragments arranged by size:
5087 765 744 495 149
StyI C'CwwG_G
Cuts at: 513 1065 2772 3596 3605 3831 4342 4679 513
Size: 552 1707 824 9 226 511 337 3074
Fragments arranged by size:
3074 1707 824 552 511 337 226 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 2802 3518 3961 4166 5267 5284
Size: 1255 789 433 716 443 205 1101 17
Cuts at: 5284 5437 5622 6067 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1101 789 716 445 443 433
205 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3693 5486 7198
Size: 274 80 33 51 1443 1716 1793 1712
Cuts at: 7198 96
Size: 138
Fragments arranged by size:
1793 1716 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4359 4391 7200 7232 4359
Size: 32 2809 32 4367
Fragments arranged by size:
4367 2809 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4333 1316
Size: 3017 4223
XbaI T'CTAG_A
Cuts at: 4576 4576
Size: 7240
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7240
XmaI C'CCGG_G
Cuts at: 1870 2045 2190 3897 4565 1870
Size: 175 145 1707 668 4545
Fragments arranged by size:
4545 1707 668 175 145
XmnI GAAnn'nnTTC
Cuts at: 5369 5369
Size: 7240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5048 276
Size: 53 83 186 936 3514 2468
Fragments arranged by size:
3514 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SexAI SfiI SwaI XcmI