Restriction map for
pFN21ASDA0677
(Circular) (Six-base) MAPSORT of: pFN21ASDA0677.seq Check: 5086 from: 1 to: 7921
pFN21ASDA0677 7921 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5731 278
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2885 5031 5264 1937
Size: 948 2146 233 4594
Fragments arranged by size:
4594 2146 948 233
Acc65I G'GTAC_C
Cuts at: 2435 5242 2435
Size: 2807 5114
AclI AA'CG_TT
Cuts at: 1563 6048 6421 1563
Size: 4485 373 3063
Fragments arranged by size:
4485 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 2610 2792 3197 3423 4083 4317 4816
Size: 454 524 182 405 226 660 234 499
Cuts at: 4816 4917 5984 7368 7636 1632
Size: 101 1067 1384 268 1917
Fragments arranged by size:
1917 1384 1067 660 524 499 454 405
268 234 226 182 101
AflII C'TTAA_G
Cuts at: 820 1017 3083 820
Size: 197 2066 5658
Fragments arranged by size:
5658 2066 197
AflIII A'CryG_T
Cuts at: 1203 3771 6820 7797 1203
Size: 2568 3049 977 1327
Fragments arranged by size:
3049 2568 1327 977
AgeI A'CCGG_T
Cuts at: 6752 6752
Size: 7921
AhdI GACnn_n'nnGTC
Cuts at: 3518 6650 3518
Size: 3132 4789
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3678 1184
Size: 2494 5427
AlwNI CAG_nnn'CTG
Cuts at: 5664 7236 5664
Size: 1572 6349
ApaI G_GGCC'C
Cuts at: 4375 4375
Size: 7921
ApaLI G'TGCA_C
Cuts at: 3653 5978 7134 3653
Size: 2325 1156 4440
Fragments arranged by size:
4440 2325 1156
ApoI r'AATT_y
Cuts at: 1493 2784 3410 3989 4064 5230 5456 5608 7717
Size: 1291 626 579 75 1166 226 152 2109
Cuts at: 7717 1493
Size: 1697
Fragments arranged by size:
2109 1697 1291 1166 626 579 226 152
75
AseI AT'TA_AT
Cuts at: 160 1032 6475 160
Size: 872 5443 1606
Fragments arranged by size:
5443 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
AvaI C'yCGr_G
Cuts at: 1870 1943 2372 5246 1870
Size: 73 429 2874 4545
Fragments arranged by size:
4545 2874 429 73
BamHI G'GATC_C
Cuts at: 5251 5618 5251
Size: 367 7554
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2435 3216 4214 5242 6697
Size: 325 217 627 648 781 998 1028 1455
Cuts at: 6697 618
Size: 1842
Fragments arranged by size:
1842 1455 1028 998 781 648 627 325
217
BanII G_rGCy'C
Cuts at: 1391 2140 2373 3264 3563 3628 4375 5240 1391
Size: 749 233 891 299 65 747 865 4072
Fragments arranged by size:
4072 891 865 749 747 299 233 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2573 2598 3185 3514 4305 4645 928
Size: 971 674 25 587 329 791 340 4204
Fragments arranged by size:
4204 971 791 674 587 340 329 25
BbvCI CC'TCA_GC
Cuts at: 3912 3912
Size: 7921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6112 6146 1903
Size: 34 4175 34 3678
Fragments arranged by size:
4175 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5813 7023 5813
Size: 1210 6711
BclI T'GATC_A
Cuts at: 1548 2826 1548
Size: 1278 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4013 4703 6532
Size: 107 122 71 631 605 2341 690 1829
Cuts at: 6532 136
Size: 1525
Fragments arranged by size:
2341 1829 1525 690 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2964 7916 2964
Size: 4952 2969
BlpI GC'TnA_GC
Cuts at: 5338 5338
Size: 7921
Bme1580I G_kGCm'C
Cuts at: 1790 3131 3314 3657 4375 5982 7138 1790
Size: 1341 183 343 718 1607 1156 2573
Fragments arranged by size:
2573 1607 1341 1156 718 343 183
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 5652 6610
Size: 416 53 83 399 24 629 3606 958
Cuts at: 6610 442
Size: 1753
Fragments arranged by size:
3606 1753 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2156 1056
Size: 1100 6821
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3631 5017 3631
Size: 1386 6535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2835 4862 6581 1239
Size: 1596 2027 1719 2579
Fragments arranged by size:
2579 2027 1719 1596
Bpu10I CC'TnA_GC
Cuts at: 2661 3912 2661
Size: 1251 6670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2790 4223 5146 6046 6911 7209 2790
Size: 1433 923 900 865 298 3502
Fragments arranged by size:
3502 1433 923 900 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3165 3992 4790 6584 882
Size: 617 1666 827 798 1794 2219
Fragments arranged by size:
2219 1794 1666 827 798 617
BsaAI yAC'GTr
Cuts at: 493 1204 5218 5712 493
Size: 711 4014 494 2702
Fragments arranged by size:
4014 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7915 7915
Size: 7921
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3808 5728 6110
Size: 53 83 186 936 1981 294 1920 382
Cuts at: 6110 6745 275
Size: 635 1451
Fragments arranged by size:
1981 1920 1451 936 635 382 294 186
83 53
BsaWI w'CCGG_w
Cuts at: 4465 6354 6752 7026 7173 7584 4465
Size: 1889 398 274 147 411 4802
Fragments arranged by size:
4802 1889 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7696
Fragments arranged by size:
7696 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3134 3137 3149 3458 3467 3929 4220 4352
Size: 1948 3 12 309 9 462 291 132
Cuts at: 4352 4380 1186
Size: 28 4727
Fragments arranged by size:
4727 1948 462 309 291 132 28 12
9 3
BseYI C'CCAG_C
Cuts at: 3023 7124 7620 3023
Size: 4101 496 3324
Fragments arranged by size:
4101 3324 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2442 2606 4264 4577 5175 1163
Size: 573 706 164 1658 313 598 3909
Fragments arranged by size:
3909 1658 706 598 573 313 164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4573 5371 6132 7160 1540
Size: 275 186 2572 798 761 1028 2301
Fragments arranged by size:
2572 2301 1028 798 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 2373 2716 2942 3264 3657 5982 6067 7138
Size: 233 343 226 322 393 2325 85 1071
Cuts at: 7138 2140
Size: 2923
Fragments arranged by size:
2923 2325 1071 393 343 322 233 226
85
BsmI GAATG_Cn'
Cuts at: 5432 5525 5432
Size: 93 7828
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2373 2716 2942 3131 3264 3314
Size: 399 350 233 343 226 189 133 50
Cuts at: 3314 3563 3628 3657 4375 5240 5982 6067 7138
Size: 249 65 29 718 865 742 85 1071
Cuts at: 7138 1391
Size: 2174
Fragments arranged by size:
2174 1071 865 742 718 399 350 343
249 233 226 189 133 85 65 50
29
BspEI T'CCGG_A
Cuts at: 7584 7584
Size: 7921
BspHI T'CATG_A
Cuts at: 5810 7540 5810
Size: 1730 6191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2628 4670 5276 844
Size: 1784 2042 606 3489
Fragments arranged by size:
3489 2042 1784 606
BsrBI CCG'CTC
Cuts at: 1394 1823 2377 3742 5808 1394
Size: 429 554 1365 2066 3507
Fragments arranged by size:
3507 2066 1365 554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2112 4449 6416 6590 65
Size: 1183 864 2337 1967 174 1396
Fragments arranged by size:
2337 1967 1396 1183 864 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2475 2852 4613 6565 6752 1700
Size: 110 665 377 1761 1952 187 2869
Fragments arranged by size:
2869 1952 1761 665 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7921
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7921
BssSI C'ACGA_G
Cuts at: 2187 3703 5981 6993 2187
Size: 1516 2278 1012 3115
Fragments arranged by size:
3115 2278 1516 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2932 3758 4242 794
Size: 2138 826 484 4473
Fragments arranged by size:
4473 2138 826 484
BstBI TT'CG_AA
Cuts at: 2007 6725 2007
Size: 4718 3203
BstEII G'GTnAC_C
Cuts at: 1184 2843 1184
Size: 1659 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 2178 2643 4940 4954 2178
Size: 465 2297 14 5145
Fragments arranged by size:
5145 2297 465 14
BstYI r'GATC_y
Cuts at: 1972 2964 3277 3481 5251 5618 6003 6020 7472
Size: 992 313 204 1770 367 385 17 1452
Cuts at: 7472 7558 7570 7916 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1770 1452 992 385 367 346 313
204 86 17 12
BstZ17I GTA'TAC
Cuts at: 5032 5032
Size: 7921
Bsu36I CC'TnA_GG
Cuts at: 3900 4851 3900
Size: 951 6970
BtgI C'CryG_G
Cuts at: 513 1065 2022 2863 2970 4002 513
Size: 552 957 841 107 1032 4432
Fragments arranged by size:
4432 1032 957 841 552 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7182
Fragments arranged by size:
7182 683 56
BtsI GCAGTG_nn'
Cuts at: 4021 4565 4719 5437 6231 6251 4021
Size: 544 154 718 794 20 5691
Fragments arranged by size:
5691 794 718 544 154 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7886
DraI TTT'AAA
Cuts at: 5225 5225
Size: 7921
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2254 4400 4934 6928 809
Size: 507 938 2146 534 1994 1802
Fragments arranged by size:
2146 1994 1802 938 534 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2473 3189 4570
Size: 54 1425 50 275 18 643 716 1381
Cuts at: 4570 4930 5368 6257 7886 8
Size: 360 438 889 1629 43
Fragments arranged by size:
1629 1425 1381 889 716 643 438 360
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4570 5368 1537
Size: 275 2758 798 4090
Fragments arranged by size:
4090 2758 798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2361 3269 3411 3435 3574 3592 3780
Size: 729 279 908 142 24 139 18 188
Cuts at: 3780 3900 5040 5851 1353
Size: 120 1140 811 3423
Fragments arranged by size:
3423 1140 908 811 729 279 188 142
139 120 24 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2726 6508 6882 7028 387
Size: 1064 138 1137 3782 374 146 1280
Fragments arranged by size:
3782 1280 1137 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2138 2371 3262 2138
Size: 233 891 6797
Fragments arranged by size:
6797 891 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2610 2792 2835 3197 3423 4083
Size: 393 454 524 182 43 362 226 660
Cuts at: 4083 4317 4816 4862 4917 5984 6581 7368 7636
Size: 234 499 46 55 1067 597 787 268
Cuts at: 7636 1239
Size: 1524
Fragments arranged by size:
1524 1067 787 660 597 524 499 454
393 362 268 234 226 182 55 46
43
EcoNI CCTnn'n_nnAGG
Cuts at: 4337 4896 4337
Size: 559 7362
EcoO109I rG'GnC_Cy
Cuts at: 4015 4276 4371 4372 4704 4015
Size: 261 95 1 332 7232
Fragments arranged by size:
7232 332 261 95 1
EcoRI G'AATT_C
Cuts at: 2784 3989 5230 5608 2784
Size: 1205 1241 378 5097
Fragments arranged by size:
5097 1241 1205 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 4723 2590
Size: 2133 5788
FspI TGC'GCA
Cuts at: 6427 6427
Size: 7921
HaeII r_GCGC'y
Cuts at: 1678 3723 4762 7068 1678
Size: 2045 1039 2306 2531
Fragments arranged by size:
2531 2306 2045 1039
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 3504 3536 3569
Size: 32 135 32 487 32 1413 32 33
Cuts at: 3569 3601 3866 3898 4039 4071 4297 4329 4811
Size: 32 265 32 141 32 226 32 482
Cuts at: 4811 4843 6562 6594 6636 6668 1373
Size: 32 1719 32 42 32 2626
Fragments arranged by size:
2626 1719 1413 487 482 265 226 141
135 42 33 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4447 4813 5265 5511 669
Size: 988 281 2509 366 452 246 3079
Fragments arranged by size:
3079 2509 988 452 366 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7921
HpaI GTT'AAC
Cuts at: 5511 5511
Size: 7921
KpnI G_GTAC'C
Cuts at: 2439 5246 2439
Size: 2807 5114
MfeI C'AATT_G
Cuts at: 5520 5520
Size: 7921
MluI A'CGCG_T
Cuts at: 7797 7797
Size: 7921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2102 2658 7035 7219 1305
Size: 797 556 4377 184 2007
Fragments arranged by size:
4377 2007 797 556 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3191 10
Size: 54 1425 343 1359 4740
Fragments arranged by size:
4740 1425 1359 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2457 2975 3660 5879 6238 6397 518
Size: 648 1291 518 685 2219 359 159 2042
Fragments arranged by size:
2219 2042 1291 685 648 518 359 159
MspA1I CmG'CkG
Cuts at: 1606 2242 3074 3473 3892 4575 5337 6014 7162
Size: 636 832 399 419 683 762 677 1148
Cuts at: 7162 7407 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 832 762 683 677 636 419
399 245
NaeI GCC'GGC
Cuts at: 2477 2854 2477
Size: 377 7544
NcoI C'CATG_G
Cuts at: 513 1065 2022 2863 2970 4002 513
Size: 552 957 841 107 1032 4432
Fragments arranged by size:
4432 1032 957 841 552 107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7921
NgoMIV G'CCGG_C
Cuts at: 2475 2852 2475
Size: 377 7544
NheI G'CTAG_C
Cuts at: 1052 2152 1052
Size: 1100 6821
NotI GC'GGCC_GC
Cuts at: 5368 5368
Size: 7921
NspI r_CATG'y
Cuts at: 1132 1229 2456 2563 3775 5095 5279 6824 1132
Size: 97 1227 107 1212 1320 184 1545 2229
Fragments arranged by size:
2229 1545 1320 1227 1212 184 107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7921
PciI A'CATG_T
Cuts at: 3771 6820 3771
Size: 3049 4872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2417 3110 1108
Size: 1309 693 5919
Fragments arranged by size:
5919 1309 693
PfoI T'CCnGG_A
Cuts at: 2490 2490
Size: 7921
PmeI GTTT'AAAC
Cuts at: 5225 5225
Size: 7921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5990 6022 7503 7535 5990
Size: 32 1481 32 6376
Fragments arranged by size:
6376 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4276 4276
Size: 7921
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7921
PsiI TTA'TAA
Cuts at: 5491 5491
Size: 7921
PspOMI G'GGCC_C
Cuts at: 4371 4371
Size: 7921
PspXI vC'TCGA_Gb
Cuts at: 2372 2372
Size: 7921
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2528 2560 2528
Size: 32 7889
PstI C_TGCA'G
Cuts at: 830 3858 4172 4380 4726 5273 830
Size: 3028 314 208 346 547 3478
Fragments arranged by size:
3478 3028 547 346 314 208
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7921
PvuII CAG'CTG
Cuts at: 2242 3074 3473 3892 2242
Size: 832 399 419 6271
Fragments arranged by size:
6271 832 419 399
SacI G_AGCT'C
Cuts at: 2140 2373 3264 2140
Size: 233 891 6797
Fragments arranged by size:
6797 891 233
SalI G'TCGA_C
Cuts at: 1936 5263 1936
Size: 3327 4594
SanDI GG'GwC_CC
Cuts at: 4276 4276
Size: 7921
SapI GCTCTTCn'nnn_
Cuts at: 2361 3269 3411 3574 5040 2361
Size: 908 142 163 1466 5242
Fragments arranged by size:
5242 1466 908 163 142
SbfI CC_TGCA'GG
Cuts at: 5273 5273
Size: 7921
ScaI AGT'ACT
Cuts at: 2339 6169 7881 2339
Size: 3830 1712 2379
Fragments arranged by size:
3830 2379 1712
SexAI A'CCwGG_T
Cuts at: 4980 4980
Size: 7921
SfcI C'TryA_G
Cuts at: 826 1045 2353 2605 2944 3504 3854 4168 4376
Size: 219 1308 252 339 560 350 314 208
Cuts at: 4376 4722 5269 6404 7085 7276 826
Size: 346 547 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 681 560 547 350 346
339 314 252 219 208 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4013 4013
Size: 7921
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7921
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7921
SmaI CCC'GGG
Cuts at: 1872 5248 1872
Size: 3376 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2372 2805 3083 4202 5161 6025
Size: 197 926 429 433 278 1119 959 864
Cuts at: 6025 6926 7188 820
Size: 901 262 1553
Fragments arranged by size:
1553 1119 959 926 901 864 433 429
278 262 197
SnaBI TAC'GTA
Cuts at: 493 5218 5712 493
Size: 4725 494 2702
Fragments arranged by size:
4725 2702 494
SpeI A'CTAG_T
Cuts at: 152 3602 152
Size: 3450 4471
SphI G_CATG'C
Cuts at: 1132 5279 1132
Size: 4147 3774
SspI AAT'ATT
Cuts at: 5 52 2470 5845 5
Size: 47 2418 3375 2081
Fragments arranged by size:
3375 2418 2081 47
StuI AGG'CCT
Cuts at: 1517 3926 1517
Size: 2409 5512
StyI C'CwwG_G
Cuts at: 513 1065 2022 2131 2157 2863 2970 3270 3477
Size: 552 957 109 26 706 107 300 207
Cuts at: 3477 3628 3801 4002 4698 5360 513
Size: 151 173 201 696 662 3074
Fragments arranged by size:
3074 957 706 696 662 552 300 207
201 173 151 109 107 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3356 3858 5948 5965 6118 6303 6748
Size: 1255 1776 502 2090 17 153 185 445
Cuts at: 6748 325
Size: 1498
Fragments arranged by size:
2090 1776 1498 1255 502 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 2337 2550
Size: 274 80 33 51 1443 297 63 213
Cuts at: 2550 6167 7879 96
Size: 3617 1712 138
Fragments arranged by size:
3617 1712 1443 297 274 213 138 80
63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7881 7913 7881
Size: 32 7889
Tth111I GACn'n_nGTC
Cuts at: 1316 3810 1316
Size: 2494 5427
XbaI T'CTAG_A
Cuts at: 5069 5257 5069
Size: 188 7733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2239
Size: 7921
XhoI C'TCGA_G
Cuts at: 1943 2372 1943
Size: 429 7492
XmaI C'CCGG_G
Cuts at: 1870 5246 1870
Size: 3376 4545
XmnI GAAnn'nnTTC
Cuts at: 3989 4096 5049 6050 3989
Size: 107 953 1001 5860
Fragments arranged by size:
5860 1001 953 107
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5729 276
Size: 53 83 186 936 4195 2468
Fragments arranged by size:
4195 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI RsrII SacII SfoI SrfI
SwaI