Restriction map for
pFN21ASDA0669
(Circular) (Six-base) MAPSORT of: pFN21ASDA0669.seq Check: 3687 from: 1 to: 7069
pFN21ASDA0669 7069 bp
With 182 enzymes: SgfI *
January 16, 2009 15:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2154 2175 4879 278
Size: 53 83 186 936 618 21 2704 2468
Fragments arranged by size:
2704 2468 936 618 186 83 53 21
AccI GT'mk_AC
Cuts at: 1937 3587 3736 3795 4412 1937
Size: 1650 149 59 617 4594
Fragments arranged by size:
4594 1650 617 149 59
Acc65I G'GTAC_C
Cuts at: 4390 4390
Size: 7069
AclI AA'CG_TT
Cuts at: 1563 5196 5569 1563
Size: 3633 373 3063
Fragments arranged by size:
3633 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3890 5132 6516 6784 1632
Size: 2258 1242 1384 268 1917
Fragments arranged by size:
2258 1917 1384 1242 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6872
AflIII A'CryG_T
Cuts at: 1203 2492 2564 4020 5968 6945 1203
Size: 1289 72 1456 1948 977 1327
Fragments arranged by size:
1948 1456 1327 1289 977 72
AgeI A'CCGG_T
Cuts at: 5900 5900
Size: 7069
AhdI GACnn_n'nnGTC
Cuts at: 5798 5798
Size: 7069
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7069
AlwNI CAG_nnn'CTG
Cuts at: 2465 2992 4812 6384 2465
Size: 527 1820 1572 3150
Fragments arranged by size:
3150 1820 1572 527
ApaI G_GGCC'C
Cuts at: 2736 2979 3053 2736
Size: 243 74 6752
Fragments arranged by size:
6752 243 74
ApaLI G'TGCA_C
Cuts at: 5126 6282 5126
Size: 1156 5913
ApoI r'AATT_y
Cuts at: 1493 4378 4604 4756 6865 1493
Size: 2885 226 152 2109 1697
Fragments arranged by size:
2885 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5623 160
Size: 872 4591 1606
Fragments arranged by size:
4591 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 2264 2735 2908 2978 4394 1870
Size: 73 235 86 471 173 70 1416 4545
Fragments arranged by size:
4545 1416 471 235 173 86 73 70
BamHI G'GATC_C
Cuts at: 4399 4766 4399
Size: 367 6702
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2678 3062 3221 3263 3529
Size: 325 217 627 891 384 159 42 266
Cuts at: 3529 4390 5845 618
Size: 861 1455 1842
Fragments arranged by size:
1842 1455 891 861 627 384 325 266
217 159 42
BanII G_rGCy'C
Cuts at: 1391 2447 2544 2736 2979 3053 3236 4388 1391
Size: 1056 97 192 243 74 183 1152 4072
Fragments arranged by size:
4072 1152 1056 243 192 183 97 74
BbeI G_GCGC'C
Cuts at: 2682 3066 3225 2682
Size: 384 159 6526
Fragments arranged by size:
6526 384 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3303 3981 928
Size: 971 1404 678 4016
Fragments arranged by size:
4016 1404 971 678
BbvCI CC'TCA_GC
Cuts at: 3102 4357 3102
Size: 1255 5814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3373 3407 5260 5294 1903
Size: 34 1436 34 1853 34 3678
Fragments arranged by size:
3678 1853 1436 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3108 4961 6171 3108
Size: 1853 1210 4006
Fragments arranged by size:
4006 1853 1210
BclI T'GATC_A
Cuts at: 1548 2514 4240 1548
Size: 966 1726 4377
Fragments arranged by size:
4377 1726 966
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2087 2402 3071
Size: 107 122 71 631 605 415 315 669
Cuts at: 3071 3245 5680 136
Size: 174 2435 1525
Fragments arranged by size:
2435 1525 669 631 605 415 315 174
122 107 71
BglII A'GATC_T
Cuts at: 7064 7064
Size: 7069
BlpI GC'TnA_GC
Cuts at: 3644 4486 3644
Size: 842 6227
Bme1580I G_kGCm'C
Cuts at: 1790 2736 2979 3053 3266 3396 3534 3564 5130
Size: 946 243 74 213 130 138 30 1566
Cuts at: 5130 6286 1790
Size: 1156 2573
Fragments arranged by size:
2573 1566 1156 946 243 213 138 130
74 30
BmgBI CAC'GTC
Cuts at: 1318 2565 1318
Size: 1247 5822
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3451 3625 4800
Size: 416 53 83 399 24 2034 174 1175
Cuts at: 4800 5758 442
Size: 958 1753
Fragments arranged by size:
2034 1753 1175 958 416 399 174 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7069
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 2236 2170
Size: 66 7003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3408 5729 1239
Size: 2169 2321 2579
Fragments arranged by size:
2579 2321 2169
Bpu10I CC'TnA_GC
Cuts at: 3102 3703 3752 4357 3102
Size: 601 49 605 5814
Fragments arranged by size:
5814 605 601 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5194 6059 6357 5194
Size: 865 298 5906
Fragments arranged by size:
5906 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5732 882
Size: 617 4233 2219
Fragments arranged by size:
4233 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3175 4366 4860 493
Size: 711 1971 1191 494 2702
Fragments arranged by size:
2702 1971 1191 711 494
BsaBI GATnn'nnATC
Cuts at: 7063 7063
Size: 7069
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 2172 2679 3063
Size: 53 83 186 936 618 21 507 384
Cuts at: 3063 3222 4876 5258 5893 275
Size: 159 1654 382 635 1451
Fragments arranged by size:
1654 1451 936 635 618 507 384 382
186 159 83 53 21
BsaWI w'CCGG_w
Cuts at: 2684 5502 5900 6174 6321 6732 2684
Size: 2818 398 274 147 411 3021
Fragments arranged by size:
3021 2818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2141 2171 2325 2328 2355
Size: 30 165 30 738 30 154 3 27
Cuts at: 2355 2358 2679 2709 3402 3432 4056 4086 4318
Size: 3 321 30 693 30 624 30 232
Cuts at: 4318 4348 1178
Size: 30 3899
Fragments arranged by size:
3899 738 693 624 321 232 165 154
30 30 30 30 30 30 30 27
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2216 2276 2349 2352 4345 1186
Size: 960 70 60 73 3 1993 3910
Fragments arranged by size:
3910 1993 960 73 70 60 3
BseYI C'CCAG_C
Cuts at: 2433 2814 2954 3084 3315 3320 3446 3774 6272
Size: 381 140 130 231 5 126 328 2498
Cuts at: 6272 6768 2433
Size: 496 2734
Fragments arranged by size:
2734 2498 496 381 328 231 140 130
126 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3440 1163
Size: 573 1704 4792
Fragments arranged by size:
4792 1704 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2557 2922 4519 5280 6308 1540
Size: 275 186 556 365 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 556 365 275 186
BsiHKAI G_wGCw'C
Cuts at: 2422 5130 5215 6286 2422
Size: 2708 85 1071 3205
Fragments arranged by size:
3205 2708 1071 85
BsmI GAATG_Cn'
Cuts at: 3280 4580 4673 3280
Size: 1300 93 5676
Fragments arranged by size:
5676 1300 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2158 2179 2225 2278 2158
Size: 21 46 53 6949
Fragments arranged by size:
6949 53 46 21
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2422 2447 2544 2736 2979 3053 3236
Size: 399 632 25 97 192 243 74 183
Cuts at: 3236 3266 3396 3534 3564 4388 5130 5215 6286
Size: 30 130 138 30 824 742 85 1071
Cuts at: 6286 1391
Size: 2174
Fragments arranged by size:
2174 1071 824 742 632 399 243 192
183 138 130 97 85 74 30 30
25
BspEI T'CCGG_A
Cuts at: 6732 6732
Size: 7069
BspHI T'CATG_A
Cuts at: 4958 6688 4958
Size: 1730 5339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3171 3355 4424 844
Size: 2327 184 1069 3489
Fragments arranged by size:
3489 2327 1069 184
BsrBI CCG'CTC
Cuts at: 1394 1823 2535 2662 2972 3566 4956 1394
Size: 429 712 127 310 594 1390 3507
Fragments arranged by size:
3507 1390 712 594 429 310 127
BsrDI GCAATG_nn'
Cuts at: 65 1248 3846 4107 4243 5564 5738 65
Size: 1183 2598 261 136 1321 174 1396
Fragments arranged by size:
2598 1396 1321 1183 261 174 136
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 3022 3179 3218 3266 5713 5900
Size: 110 225 987 157 39 48 2447 187
Cuts at: 5900 1700
Size: 2869
Fragments arranged by size:
2869 2447 987 225 187 157 110 48
39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7069
BssHII G'CGCG_C
Cuts at: 1925 3291 1925
Size: 1366 5703
BssSI C'ACGA_G
Cuts at: 5129 6141 5129
Size: 1012 6057
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7069
BstBI TT'CG_AA
Cuts at: 2007 5873 2007
Size: 3866 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7069
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 3692 2383
Size: 1309 5760
BstYI r'GATC_y
Cuts at: 1972 2612 4105 4399 4766 5151 5168 6620 6706
Size: 640 1493 294 367 385 17 1452 86
Cuts at: 6706 6718 7064 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1493 1452 640 385 367 346 294
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2199 2199
Size: 7069
BtgI C'CryG_G
Cuts at: 513 1065 2076 2184 2527 3069 3240 3258 3330
Size: 552 1011 108 343 542 171 18 72
Cuts at: 3330 3525 513
Size: 195 4057
Fragments arranged by size:
4057 1011 552 542 343 195 171 108
72 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6330
Fragments arranged by size:
6330 683 56
BtsI GCAGTG_nn'
Cuts at: 3063 3493 4308 4585 5379 5399 3063
Size: 430 815 277 794 20 4733
Fragments arranged by size:
4733 815 794 430 277 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7034
DraI TTT'AAA
Cuts at: 4158 4373 4158
Size: 215 6854
DraIII CAC_nnn'GTG
Cuts at: 3526 3526
Size: 7069
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 2767 6076 809
Size: 507 1284 167 3309 1802
Fragments arranged by size:
3309 1802 1284 507 167
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2088 2554
Size: 54 1425 50 275 18 207 51 466
Cuts at: 2554 3148 3177 3246 3268 3930 4516 5405 7034
Size: 594 29 69 22 662 586 889 1629
Cuts at: 7034 8
Size: 43
Fragments arranged by size:
1629 1425 889 662 594 586 466 275
207 69 54 51 50 43 29 22
18
EagI C'GGCC_G
Cuts at: 1537 1812 2554 4516 1537
Size: 275 742 1962 4090
Fragments arranged by size:
4090 1962 742 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2223 2227 4999 1353
Size: 689 181 4 2772 3423
Fragments arranged by size:
3423 2772 689 181 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2440 3459 5656 6030 6176 387
Size: 1064 138 851 1019 2197 374 146 1280
Fragments arranged by size:
2197 1280 1064 1019 851 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3408 3890 5132 5729 6516 6784 1239
Size: 393 1776 482 1242 597 787 268 1524
Fragments arranged by size:
1776 1524 1242 787 597 482 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2975 3050 2975
Size: 75 6994
EcoRI G'AATT_C
Cuts at: 4378 4756 4378
Size: 378 6691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2252 4209 2071
Size: 181 1957 4931
Fragments arranged by size:
4931 1957 181
FseI GG_CCGG'CC
Cuts at: 3183 3183
Size: 7069
FspI TGC'GCA
Cuts at: 5575 5575
Size: 7069
HaeII r_GCGC'y
Cuts at: 1678 2372 2682 3066 3225 3710 6216 1678
Size: 694 310 384 159 485 2506 2531
Fragments arranged by size:
2531 2506 694 485 384 310 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2325 2357 4040
Size: 32 135 32 569 32 152 32 1683
Cuts at: 4040 4072 4232 4264 5710 5742 5784 5816 1373
Size: 32 160 32 1446 32 42 32 2626
Fragments arranged by size:
2626 1683 1446 569 160 152 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3796 3912 4413 4659 669
Size: 988 281 1858 116 501 246 3079
Fragments arranged by size:
3079 1858 988 501 281 246 116
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7069
HpaI GTT'AAC
Cuts at: 3912 4659 3912
Size: 747 6322
KasI G'GCGC_C
Cuts at: 2678 3062 3221 2678
Size: 384 159 6526
Fragments arranged by size:
6526 384 159
KpnI G_GTAC'C
Cuts at: 4394 4394
Size: 7069
MfeI C'AATT_G
Cuts at: 4668 4668
Size: 7069
MluI A'CGCG_T
Cuts at: 6945 6945
Size: 7069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 6183 6367 1305
Size: 1461 3417 184 2007
Fragments arranged by size:
3417 2007 1461 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 3932 10
Size: 54 1425 343 258 1842 3147
Fragments arranged by size:
3147 1842 1425 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5027 5386 5545 518
Size: 648 3861 359 159 2042
Fragments arranged by size:
3861 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2302 2309 2670 2896 3071 3496 3643 4485
Size: 696 7 361 226 175 425 147 842
Cuts at: 4485 5162 6310 6555 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 842 696 677 425 361 245
226 175 147 7
NaeI GCC'GGC
Cuts at: 2037 3024 3181 2037
Size: 987 157 5925
Fragments arranged by size:
5925 987 157
NarI GG'CG_CC
Cuts at: 2679 3063 3222 2679
Size: 384 159 6526
Fragments arranged by size:
6526 384 159
NcoI C'CATG_G
Cuts at: 513 1065 3330 513
Size: 552 2265 4252
Fragments arranged by size:
4252 2265 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7069
NgoMIV G'CCGG_C
Cuts at: 2035 3022 3179 2035
Size: 987 157 5925
Fragments arranged by size:
5925 987 157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7069
NotI GC'GGCC_GC
Cuts at: 4516 4516
Size: 7069
NspI r_CATG'y
Cuts at: 1132 1229 2496 3692 4427 5972 1132
Size: 97 1267 1196 735 1545 2229
Fragments arranged by size:
2229 1545 1267 1196 735 97
PasI CC'CwG_GG
Cuts at: 1348 2965 1348
Size: 1617 5452
PciI A'CATG_T
Cuts at: 2492 5968 2492
Size: 3476 3593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7069
PmeI GTTT'AAAC
Cuts at: 4373 4373
Size: 7069
PmlI CAC'GTG
Cuts at: 3175 3175
Size: 7069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5138 5170 6651 6683 5138
Size: 32 1481 32 5524
Fragments arranged by size:
5524 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7069
PsiI TTA'TAA
Cuts at: 4639 4639
Size: 7069
PspOMI G'GGCC_C
Cuts at: 2732 2975 3049 2732
Size: 243 74 6752
Fragments arranged by size:
6752 243 74
PstI C_TGCA'G
Cuts at: 830 2470 3058 3168 3189 3556 4421 830
Size: 1640 588 110 21 367 865 3478
Fragments arranged by size:
3478 1640 865 588 367 110 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7069
PvuII CAG'CTG
Cuts at: 2302 3643 2302
Size: 1341 5728
SacII CC_GC'GG
Cuts at: 3072 3072
Size: 7069
SalI G'TCGA_C
Cuts at: 1936 3794 4411 1936
Size: 1858 617 4594
Fragments arranged by size:
4594 1858 617
SapI GCTCTTCn'nnn_
Cuts at: 2042 2227 2042
Size: 185 6884
SbfI CC_TGCA'GG
Cuts at: 4421 4421
Size: 7069
ScaI AGT'ACT
Cuts at: 2641 5317 7029 2641
Size: 2676 1712 2681
Fragments arranged by size:
2681 2676 1712
SexAI A'CCwGG_T
Cuts at: 3508 3508
Size: 7069
SfcI C'TryA_G
Cuts at: 826 1045 2466 2545 3054 3164 3185 3552 4417
Size: 219 1421 79 509 110 21 367 865
Cuts at: 4417 5552 6233 6424 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1421 1135 865 681 509 367 219
191 110 79 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2087 2087
Size: 7069
SfoI GGC'GCC
Cuts at: 2680 3064 3223 2680
Size: 384 159 6526
Fragments arranged by size:
6526 384 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
SgrAI Cr'CCGG_yG
Cuts at: 1700 3218 1700
Size: 1518 5551
SmaI CCC'GGG
Cuts at: 1872 2266 2910 2980 4396 1872
Size: 394 644 70 1416 4545
Fragments arranged by size:
4545 1416 644 394 70
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5173 6074 6336 820
Size: 197 926 3230 901 262 1553
Fragments arranged by size:
3230 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4366 4860 493
Size: 3873 494 2702
Fragments arranged by size:
3873 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7069
SphI G_CATG'C
Cuts at: 1132 4427 1132
Size: 3295 3774
SspI AAT'ATT
Cuts at: 5 52 4993 5
Size: 47 4941 2081
Fragments arranged by size:
4941 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7069
StyI C'CwwG_G
Cuts at: 513 1065 3330 4508 513
Size: 552 2265 1178 3074
Fragments arranged by size:
3074 2265 1178 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3422 3470 5096 5113 5266 5451 5896
Size: 1255 1842 48 1626 17 153 185 445
Cuts at: 5896 325
Size: 1498
Fragments arranged by size:
1842 1626 1498 1255 445 185 153 48
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2639 5315 7027
Size: 274 80 33 51 1443 662 2676 1712
Cuts at: 7027 96
Size: 138
Fragments arranged by size:
2676 1712 1443 662 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2679 2711 3402 3434 7029 7061 2679
Size: 32 691 32 3595 32 2687
Fragments arranged by size:
3595 2687 691 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7069
XbaI T'CTAG_A
Cuts at: 4405 4405
Size: 7069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 3256 3328 2083
Size: 1173 72 5824
Fragments arranged by size:
5824 1173 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7069
XmaI C'CCGG_G
Cuts at: 1870 2264 2908 2978 4394 1870
Size: 394 644 70 1416 4545
Fragments arranged by size:
4545 1416 644 394 70
XmnI GAAnn'nnTTC
Cuts at: 5198 5198
Size: 7069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2152 2173 4877 276
Size: 53 83 186 936 618 21 2704 2468
Fragments arranged by size:
2704 2468 936 618 186 83 53 21
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BstZ17I EcoICRI EcoNI EcoRV FspAI NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacI SanDI SrfI
SwaI